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- 저자 : Wenxu Zhu (검색결과 3 건)
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Jinle Xiang,Wenxue Zhu,Junqi Han,Zhixi Li,Hanjin Ge,Dehui Lin 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.4
Chinese raisin tree (Hovenia dulcis) peduncle is a wonderful food material for fermented food for high contents of sugars and a variety of organic acids. The organic acids in the fresh peduncle, fermented wine, and vinegar were identified and quantified by HPLC, and changes in biochemical parameters and organic acids in the fermentation process were studied. Skin-on fermentation was carried out in the alcoholic fermentation, and 9identified organic acids fluctuated by physical or biochemical reactions during the alcoholic fermentation. Submerged fermentation was carried out in the acetic fermentation. It took 72 h to finish the 1st cycle of acetification with the titratable acidity 5.42%(w/v), and 30h to finish the 2nd cycle. The contents of acetic acid, formic acid, and α-ketoglutaric acid increased, while the rest identified organic acids decreased in the acetification process. The fermentation developed in this study appeared to be a practical processing method for Chinese raisin tree peduncles.
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Liu Feifei,Li Diancheng,Xin Yuwei,Liu Fang,Li Wenxue,Zhu Jiaan 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.2
Objective: Viscoelasticity is an essential feature of nerves, although little is known about their viscous properties. The discovery of shear wave dispersion (SWD) imaging has presented a new approach for the non-invasive evaluation of tissue viscosity. The present study investigated the feasibility of using SWD imaging to evaluate diabetic neuropathy using the sciatic nerve in a diabetic rat model. Materials and Methods: This study included 11 diabetic rats in the diabetic group and 12 healthy rats in the control group. Bilateral sciatic nerves were evaluated 3 months after treatment with streptozotocin. We measured the nerve cross-sectional area (CSA), nerve stiffness using shear wave elastography (SWE), and nerve viscosity using SWD imaging. The motor nerve conduction velocity (MNCV) was also measured. These four indicators and the histology of the sciatic nerves were then compared between the two groups. The performance of CSA, SWE, and SWD imaging in distinguishing the two groups was assessed using receiver operating characteristic (ROC) analysis. Results: Nerve CSA, stiffness, and viscosity in the diabetic group was significantly higher than those in the control group (all p < 0.05). The results also revealed a significantly lower MNCV in the diabetic group (p = 0.005). Additionally, the density of myelinated fibers was significantly lower in the diabetic group (p = 0.004). The average thickness of the myelin sheath was also lower in the diabetic group (p = 0.012). The area under the ROC curve for distinguishing the diabetic neuropathy group from the control group was 0.876 for SWD imaging, which was significantly greater than 0.677 for CSA (p = 0.030) and 0.705 for SWE (p = 0.035). Conclusion: Sciatic nerve viscosity measured using SWD imaging was significantly higher in diabetic rats. The viscosity measured using SWD imaging performed well in distinguishing the diabetic neuropathy group from the control group. Therefore, SWD imaging may be a promising method for the evaluation of diabetic neuropathy.
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Dan Wang,Xin Wen,Xinyu Zhang,Yadong Hu,Xinru Li,Wenxu Zhu,Tao Wang,Shaowu Yin 한국유전학회 2018 Genes & Genomics Vol.40 No.11
Hypoxia seriously affects the innate immune system of fish. However, the roles of suppressor of cytokine signaling (SOCS), pivotal anti-inflammatory genes, in response to hypoxia/reoxygenation remain largely unexplored. The primary objective of this study was to elucidate the function of SOCS genes under acute hypoxia and reoxygenation in pufferfish (Takifugu fasciatus). In the present study, SOCS1, 2 and 3 were identified in T. fasciatus referred to as TfSOCS1, 2 and 3. Then, qRTPCR and western blot analysis were employed to assess their expressions at both the mRNA and protein levels. Tissue distribution demonstrated that the three SOCS genes were predominantly distributed in gill, brain and liver. Under hypoxia challenge (1.63 ± 0.2 mg/L DO for 2, 4, 6 and 8 h), the expressions of TfSOCS1 and 3 in brain and liver at the mRNA and protein levels were significantly decreased, while their expressions showed an opposite trend in gill. Different from the expressions of TfSOCS1 and 3, the expression of TfSOCS2 was inhibited in gill, along with its increased expression in brain and liver. After normoxic recovery (7.0 ± 0.3 mg/L of DO for 4 and 12 h), most of TfSOCS genes were significantly altered at R4 (reoxygenation for 4 h) and returned to the normal level at R12 (reoxygenation for 12 h). SOCS genes played vital roles in response to hypoxia/reoxygenation challenge. Our findings greatly strengthened the relation between innate immune and hypoxia stress in T. fasciatus.
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