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RISS 인기검색어
- 검색키워드
- 저자 : Waqas WAKIL (검색결과 12 건)
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Wakil Waqas,Kavallieratos Nickolas G.,Eleftheriadou Nikoleta,Riasat Tahira,Rasool Khawaja G.,Husain Mureed,Aldawood Abdulrahman S. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
The utilization of diatomaceous earths (DEs) has been considered as a potential alternative to traditional in secticides for controlling grain storage insect pests. In this study, 19 different commercial DEs obtained from various regions of the world were evaluated for their effectiveness to manage Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), in vitro. Two different series of bioassays were carried out, with the first screening all 19 DEs and the second evaluating the eight most effective ones at different doses and exposure intervals for mortality assessment and progeny production. Four enhanced DEs, namely InertPMS, DEA, DiaHerb, and DEBBM were highly effective compared to other DEs tested. InertPMS exhibited complete adult mortality at 50 mg/kg, followed by DEA at 75 mg/kg, DiaHerb at 100 mg/kg, and DEBBM at 125 mg/kg, while GrainGuard exhibited 94.52 % at 125 mg/kg, 14 days post-exposure. Protect-It, SilicoSec, and DiaFil 610 were less effective at 400 mg/ kg, causing 87.3 %, 84.4 %, and 79.2 % mortality after 14 days, respectively. The offspring was suppressed in wheat treated with InertPMS at 75 mg/kg, while DEA, DiaHerb, and DEBBM revealed no progeny emergence at 125 mg/kg. GrainGuard, Protect-It, SilicoSec, and DiaFil 610 were not successful at progeny suspension after 62 days. These findings suggest that the use of specific DEs at low doses could be a viable option to mitigate the use of conventional insecticides and diminish the residues of insecticides on stored grains, making them the most feasible components for integrated pest management (IPM) in stored grain systems.
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Waqas Wakil,M. Usman GHAZANFAR,권용정,Mirza A. QAYYUM,Fozia NASIR 한국곤충학회 2010 Entomological Research Vol.40 No.6
A survey was conducted in tomato fields at different localities to assess the distribution of tomato fruitworm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) in Punjab Province, Pakistan. The effect of weather factors on its population was also investigated. Eleven tomato-growing districts were selected and data for larval population and fruit infestation were recorded. The maximum larval population (5.2 larvae/plant) of H. armigera was recorded in Bahawalpur district with 32.6% fruit infestation. However, the minimum larval population (1.4 larvae/plant) inflicted 14.7% fruit infestation in Rawalpindi district. The temperature was positively correlated while relative humidity showed negative interaction with the larval population and fruit infestation.
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Waqas Wakil,M. Usman GHAZANFAR,Shahbaz Talib Sahi,권용정,M. Abdul Qayyum 한국곤충학회 2011 Entomological Research Vol.41 No.3
The efficacy of one new modified and two old meridic diets on Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) for rearing six successive generations was studied. Duration of larval development for insects fed on the modified diet was considerably shortened as most of them went through only five stadia before pupation, while the per cent pupation and per cent eclosion were relatively higher than on other diets. The lowest pupal mortality (6.33 ± 0.13%) was recorded in the F1 generation reared on the modified diet, whereas the highest pupal mortality (19.49 ± 0.15%) was observed in insects reared on a natural diet in the F6 generation. Blending of chickpea Cicer arietinum L. and red kidney bean Phaseolus vulgaris L. flours with tomato paste proved highly favorable for adult reproduction. These results suggest that the vitality of the tomato fruitworm did not decline obviously after rearing on a modified diet for several generations.
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Occurrence and diversity of entomopathogenic fungi in cultivated and uncultivated soils in Pakistan
Waqas Wakil,M. Usman GHAZANFAR,Tahira RIASAT,권용정,Mirza Abdul QAYYUM,Muhammad YASIN 한국곤충학회 2013 Entomological Research Vol.43 No.1
The distribution of entomopathogenic fungi in various geographical areas of Punjab, Pakistan, is poorly understood. The present study was planned to explore the occurrence and diversity of entomopathogenic fungi from soils collected from cultivated and non‐cultivated habitats. The detailed survey of different habitats (crop fields, fruits, vegetables, forests) was conducted to collect soil samples and the associated fungi were isolated using Galleria bait method. Among 210 soil samples, 168 fungal isolates were recovered and identified, with 98 from forests, 32 from vegetables, 30 from field crops and 8 from fruits. The major entomopathogenic fungi recovered from these samples were Beauveria bassiana, Metarhizium anisopliae, Paecilomyces lilacinus, B. brongniartii, P. chlamydosporia and Lecanicillium attenuatum. The diversity of entomopathogenic fungi was greater in soil samples from forests compared to crop fields, vegetables and fruits, respectively. The geographical attributes (altitude, longitude, latitude) greatly influenced the occurrence of entomopathogenic fungi with the highest number of isolates found from >600 m altitude, 33°–34′N latitude, and 73°74′E longitude from soil samples. The results of the surveys clearly indicated that the entomopathogenic fungi are distributed in the soils which may be used in successful IPM programs in future.
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Waqas Wakil,Tahria Riasat,M. Usman GHAZANFAR,권용정,Farid Asif Shaheen 한국곤충학회 2011 Entomological Research Vol.41 No.6
A diatomaceous earth formulation enhanced with bitterbarkomycin (DEBBM)combined with Beauveria bassiana (Balsamo) Vuillemin was evaluated against lesser grain borer Rhyzopertha dominica F. (Coleoptera: Bostrychidae) under laboratory conditions. DEBBM was applied at the rates of 15 and 30 ppm alone as well as in combination with 6.69 × 10^6, 6.69 × 10^8 and 6.69 × 10^10 conidia/kg of wheat. Mortality of treated adults was recorded after 5, 10 and 15 days of exposure. Bioassays were carried out at 20, 25 and 30°C with 55 and 75% relative humidity. The emergence of progeny was also assessed 60 days post exposure. The combined use of DEBBM and B. bassiana considerably increased adult mortality especially at increasing temperatures and longer exposure intervals compared with DEBBM and B. bassiana alone. Progeny production was less in wheat treated with high dose rates of DEBBM + B. bassiana. The per cent mycosis in the cadavers was maximum where B. bassiana was applied at low dose rates. The results of the present study indicated that a combination of DEBBM and B. bassiana may provide effective control of R. dominica.
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Waqas WAKIL,Muhammad ASHFAQ,권용정,M. U. GHAZANFAR 한국곤충학회 2009 Entomological Research Vol.39 No.1
A study was carried out at the University of Arid Agriculture, Rawalpindi, Pakistan to integrate different control measures against Helicoverpa armigera (Hübner) on chickpea, to minimize the use of hazardous insecticides and develop an eco-friendly management program. Cultural (weeding), mechanical (hand picking), biological (release of Trichogramma chilonis), microbial (spraying of Bacillus thruingiensis) and chemical (Steward) control practices were applied three times at 10 day intervals individually and also in various combinations. The application of Steward proved most effective when applied alone, with 0.41 larvae/plant, pod infestation of 9.31% and the highest grain yield (1203.66 g/plot); however, the integration of weeding, hand picking and Steward proved to be the most effective in reducing the larval population (0.12 larvae/plant) with minimum pod infestation (5.45%) on variety CM-2000, which resulted in the maximum grain yield (1260.33 g/plot). The cost–enefit ratio in the treatment where Steward was applied alone was 1:2.20, and it was 1:3.53 where weeding and hand picking practices were integrated.
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Tahira Riasat,Waqas Wakil,Muhammad YASIN,권용정 한국곤충학회 2013 Entomological Research Vol.43 No.4
The virulence of Isaria fumosorosea alone and integrated with an enhanced formulation of diatomaceous earth (diatomaceous earth + bitterbarkomycin, DEBBM) was tested against the lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) under laboratory conditions on stored wheat. The fungus was applied to wheat grains at various concentrations of 3.60 × 106, 3.60 × 108, and 3.60 × 1010 conidia/kg alone, or in combination with 15 and 30 ppm of DEBBM. R. dominica was held at 20, 25 and 30°C and two RH regimes (56 and 75%) and mortality was registered after 5, 10 and 15 d. Generally, I. fumosorosea was most effective at 25°C caused the highest mortality of R. dominica at 56% RH. The increase of temperature, concentration and exposure interval increased the insecticidal effect of DEBBM at low RH level. The DE alone suppressed the progeny emergence at higher dose rate as compared I. fumosorosea alone, but their simultaneous use further reduced the progeny production of R. dominica. The integrated use of both tested bio control agents synergized the effect of each other and caused the highest mortality at 25°C and 56% RH. The results clearly demonstrated that I. fumosorosea and new enhanced DEBBM can be integrated to be an effective control measure for R. dominica in stored wheat.
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Sohail AHMED1,Waqas WAKIL,David MANTLE,Richard M. WILKINS,권용정 한국곤충학회 2008 Entomological Research Vol.38 No.3
To investigate the high activity of intracellular proteases in insecticide resistant strains of Musca domestica L., purification by anion-exchange chromatography and gel filtration of one of the enzymes, alanyl aminopeptidase (Ala AP), in three strains of Musca domestica was carried out. The fractions collected by gel filtration of soluble homogenates of the three strains (571ab, 17bb and Cooper) showed a single peak of Ala AP activity. Partially purified Ala AP of the three strains showed high activity at pH 7.5. The presence or absence of Ca2+ in the assay medium did not produce any difference in activity of Ala AP in the 571ab and Cooper strains, but there was a significant difference in the 17bb strain. The activity of Ala AP in all three strains was essentially unaltered in the presence of inhibitors of serine (PMSF), cysteine (E-64) proteases and carboxypeptidases (pepstatin). Ala AP hydrolyzed alanine amino methylcoumarin (Ala-AMC) maximally, followed by phenyl alanine amino methylcoumarin (Phe-AMC), leucyl amino methylcoumarin (Leu-AMC) and ornithine amino methylcoumarin (Orn-AMC). Ala AP from the three strains showed differential activity towards various substrates. The comparison of alanyl aminopeptidase’'s activity from different sources is discussed.
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Muhammad Yasin,Waqas Wakil,Hamadttu A.F. EL-SHAFIE,Geoffrey O. BEDFORD,Thomas A. Miller 한국곤충학회 2017 Entomological Research Vol.47 No.4
The invasive Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae), is one of the most destructive pests of ornamental and economically important palms globally. It has been found in 50 % of date‐growing and 15 % of coconut‐producing countries in the world. Synthetic organic insecticides have been the default method to combat this pest, but they are clearly inefficient due to the secretive nature of the insect and there is concern about non‐target effects from blanket spraying. For this reason, there is increasing interest in biological control methods such as the possible use of microbial entomopathogens, which might be incorporated into IPM approaches. In this review we summarize research work on microbial control agents, their effectiveness against RPW and their integration with other control measures.
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