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RISS 인기검색어
- 검색키워드
- 저자 : M. Usman GHAZANFAR (검색결과 4 건)
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Occurrence and diversity of entomopathogenic fungi in cultivated and uncultivated soils in Pakistan
Waqas Wakil,M. Usman GHAZANFAR,Tahira RIASAT,권용정,Mirza Abdul QAYYUM,Muhammad YASIN 한국곤충학회 2013 Entomological Research Vol.43 No.1
The distribution of entomopathogenic fungi in various geographical areas of Punjab, Pakistan, is poorly understood. The present study was planned to explore the occurrence and diversity of entomopathogenic fungi from soils collected from cultivated and non‐cultivated habitats. The detailed survey of different habitats (crop fields, fruits, vegetables, forests) was conducted to collect soil samples and the associated fungi were isolated using Galleria bait method. Among 210 soil samples, 168 fungal isolates were recovered and identified, with 98 from forests, 32 from vegetables, 30 from field crops and 8 from fruits. The major entomopathogenic fungi recovered from these samples were Beauveria bassiana, Metarhizium anisopliae, Paecilomyces lilacinus, B. brongniartii, P. chlamydosporia and Lecanicillium attenuatum. The diversity of entomopathogenic fungi was greater in soil samples from forests compared to crop fields, vegetables and fruits, respectively. The geographical attributes (altitude, longitude, latitude) greatly influenced the occurrence of entomopathogenic fungi with the highest number of isolates found from >600 m altitude, 33°–34′N latitude, and 73°74′E longitude from soil samples. The results of the surveys clearly indicated that the entomopathogenic fungi are distributed in the soils which may be used in successful IPM programs in future.
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Waqas Wakil,M. Usman GHAZANFAR,Shahbaz Talib Sahi,권용정,M. Abdul Qayyum 한국곤충학회 2011 Entomological Research Vol.41 No.3
The efficacy of one new modified and two old meridic diets on Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) for rearing six successive generations was studied. Duration of larval development for insects fed on the modified diet was considerably shortened as most of them went through only five stadia before pupation, while the per cent pupation and per cent eclosion were relatively higher than on other diets. The lowest pupal mortality (6.33 ± 0.13%) was recorded in the F1 generation reared on the modified diet, whereas the highest pupal mortality (19.49 ± 0.15%) was observed in insects reared on a natural diet in the F6 generation. Blending of chickpea Cicer arietinum L. and red kidney bean Phaseolus vulgaris L. flours with tomato paste proved highly favorable for adult reproduction. These results suggest that the vitality of the tomato fruitworm did not decline obviously after rearing on a modified diet for several generations.
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Waqas Wakil,M. Usman GHAZANFAR,권용정,Mirza A. QAYYUM,Fozia NASIR 한국곤충학회 2010 Entomological Research Vol.40 No.6
A survey was conducted in tomato fields at different localities to assess the distribution of tomato fruitworm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) in Punjab Province, Pakistan. The effect of weather factors on its population was also investigated. Eleven tomato-growing districts were selected and data for larval population and fruit infestation were recorded. The maximum larval population (5.2 larvae/plant) of H. armigera was recorded in Bahawalpur district with 32.6% fruit infestation. However, the minimum larval population (1.4 larvae/plant) inflicted 14.7% fruit infestation in Rawalpindi district. The temperature was positively correlated while relative humidity showed negative interaction with the larval population and fruit infestation.
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Waqas Wakil,Tahria Riasat,M. Usman GHAZANFAR,권용정,Farid Asif Shaheen 한국곤충학회 2011 Entomological Research Vol.41 No.6
A diatomaceous earth formulation enhanced with bitterbarkomycin (DEBBM)combined with Beauveria bassiana (Balsamo) Vuillemin was evaluated against lesser grain borer Rhyzopertha dominica F. (Coleoptera: Bostrychidae) under laboratory conditions. DEBBM was applied at the rates of 15 and 30 ppm alone as well as in combination with 6.69 × 10^6, 6.69 × 10^8 and 6.69 × 10^10 conidia/kg of wheat. Mortality of treated adults was recorded after 5, 10 and 15 days of exposure. Bioassays were carried out at 20, 25 and 30°C with 55 and 75% relative humidity. The emergence of progeny was also assessed 60 days post exposure. The combined use of DEBBM and B. bassiana considerably increased adult mortality especially at increasing temperatures and longer exposure intervals compared with DEBBM and B. bassiana alone. Progeny production was less in wheat treated with high dose rates of DEBBM + B. bassiana. The per cent mycosis in the cadavers was maximum where B. bassiana was applied at low dose rates. The results of the present study indicated that a combination of DEBBM and B. bassiana may provide effective control of R. dominica.
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