Effects of different diets on the growth and development of young silkworms

Wang Yuanfei,Shu Qilong,Gu Haoyi,Feng Piao,Dai Minli,Zhu Qingyu,Liu Wei,Dai Yan,Li Fanchi,Li Bing 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

To investigate the impact of different diets on the growth and development of the silkworm Bombyx mori (L.; Lepidoptera: Bombycidae), we conducted an analysis of differentially expressed genes (DEGs) between larvae fed an artificial diet (referred to as the AD group) and those fed mulberry leaves (referred to as the ML group) during the first, second, and third instars using RNA sequencing (RNA-Seq) technology. Our results revealed that the DEGs primarily belonged to pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, the endocrine system, the nervous system, the digestive system, the immune system, and oxida tion–reduction processes. Notably, in the AD group, there was a decrease in transcript levels of genes related to amino acid metabolism synthases, while the transcript levels of antimicrobial peptide genes were up-regulated. Furthermore, genes associated with cytochrome P450 (CYP) enzymes exhibited down-regulation in the AD group. These observed changes likely contributed to the delayed growth and compromised robustness observed in the AD group. Overall, these findings provided valuable insights into the mechanisms underlying the differ ences in growth, development, disease resistance, and adversity resistance between silkworms fed an artificial diet and those fed mulberry leaves.

Exosomal CircPRRX1 Enhances Doxorubicin Resistance in Gastric Cancer by Regulating MiR-3064-5p/PTPN14 Signaling

Shumin Wang,Mei Ping,Bin Song,Yarong Guo,Yuanfei Li,Junmei Jia 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.9

Purpose: Gastric cancer (GC) is a malignant tumor with a high mortality rate. Drug resistance is a major obstacle to GC therapy. This study aimed to investigate the role and mechanism of exosomal circPRRX1 in doxorubicin resistance in GC. Materials and Methods: HGC-27 and AGS cells were exposed to different doses of doxorubicin to construct doxorubicin-resistantcell lines. Levels of circPRRX1, miR-3064-5p, and nonreceptor tyrosine phosphatase 14 (PTPN14) were detected by quantitativereal-time PCR or Western blot assay. Then, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, transwell,and Western blot assays were used to explore the function of circPRRX1 in GC cells. Interactions among circPRRX1, miR-3064-5p,and PTPN14 were confirmed by dual-luciferase reporter assay. The in vivo function of circPRRX1 was analyzed in a xenograft tumormodel. Results: CircPRRX1 was highly expressed in doxorubicin-resistant GC cell lines. Knockdown of circPRRX1 reversed doxorubicinresistance in doxorubicin-resistant GC cells. Additionally, extracellular circPRRX1 was carried by exosomes to spread doxorubicinresistance. CircPRRX1 silencing reduced doxorubicin resistance by targeting miR-3064-5p or regulating PTPN14. In GC patients,high levels of circPRRX1 in serum exosomes were associated with poor responses to doxorubicin treatment. Moreover, depletionof circPRRX1 reduced doxorubicin resistance in vivo. Conclusion: CircPRRX1 strengthened doxorubicin resistance by modulating miR-3064-5p/PTPN14 signaling and might be atherapeutic target for GC patients.

A study of predicting irradiation-induced transition temperature shift for RPV steels with XGBoost modeling

Xu, Chaoliang,Liu, Xiangbing,Wang, Hongke,Li, Yuanfei,Jia, Wenqing,Qian, Wangjie,Quan, Qiwei,Zhang, Huajian,Xue, Fei Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.8

The prediction of irradiation-induced transition temperature shift for RPV steels is an important method for long term operation of nuclear power plant. Based on the irradiation embrittlement data, an irradiation-induced transition temperature shift prediction model is developed with machine learning method XGBoost. Then the residual, standard deviation and predicted value vs. measured value analysis are conducted to analyze the accuracy of this model. At last, Cu content threshold and saturation values analysis, temperature dependence, Ni/Cu dependence and flux effect are given to verify the reliability. Those results show that the prediction model developed with XGBoost has high accuracy for predicting the irradiation embrittlement trend of RPV steel. The prediction results are consistent with the current understanding of RPV embrittlement mechanism.

Development and application of rock breaking platform with variable cross section extrusion pulsed water jet

Yangkai Zhang,Yiyu Lu,Jiren Tang,Yuanfei Ling,Lei Wang,Qi Yao,Zhidan Zhu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.6

Based on the theory of hydraulic self-reversing and pressurization, a variable cross-section squeeze pulse water jet rock breaking platform was built. When the nozzle diameter is 0.5 mm, the granite erosion and crushing experiments under different driving pressures are carried out, and the depth, volume and specific energy consumption of the erosion pit are analyzed. The results show that the device can achieve intermittent injection after pressurizing the input fluid at a rate of 5.8 times. Meanwhile, the pressurization process of fluid is divided into ascending section, stable section and descending section. Compared with the continuous water jet with the same peak output pressure, the depth of destroyed granite is increased by 129 %-288 %, the volume of broken granite is increased by 37 %-121 %, and the specific energy consumption per unit of crushing volume is reduced by 21 %-74 %. The device can use low-pressure fluid to effectively crush granite.

Efficacy of Different Number of XELOX or SOX Chemotherapy Cycles After D₂ Resection for Stage III Gastric Cancer

Yu, Yuanyuan,Zhang, Zicheng,Meng, Qianhao,Wang, Ke,Li, Qingwei,Ma, Yue,Yao, Yuanfei,Sun, Jie,Wang, Guangyu The Korean Gastric Cancer Association 2022 Journal of gastric cancer Vol.22 No.2

Purpose: We aimed to explore whether the prognosis of patients treated with capecitabine and oxaliplatin (XELOX) or S-1 and oxaliplatin (SOX) regimens who received fewer cycles of chemotherapy after D2 radical resection for gastric cancer (GC) would be non-inferior to that of patients who received the standard number of cycles of chemotherapy. Materials and Methods: Data on patients who received XELOX or SOX chemotherapy after undergoing D2 radical resection at Harbin Medical University Cancer Hospital between January 2011 and May 2016 were collected. Results: In patients who received 4, 6, and 8 cycles of chemotherapy, the 5-year overall survival (OS) rates were 59.4%, 64.8%, and 62.7%, respectively. Compared to patients who received 4 cycles of chemotherapy, those who received 6 cycles (hazard ratio [HR], 0.882; 95% confidence interval [CI], 0.599-1.299; P=0.52) or 8 cycles (HR, 0.882; 95% CI, 0.533-1.458; P=0.62) of chemotherapy did not exhibit significantly prolonged OS. The 3-year disease-free survival (DFS) rate of patients who received 4, 6, and 8 cycles of chemotherapy was 62.1%, 67.2%, and 60.8%, respectively. Compared to patients who received 4 cycles of chemotherapy, those who received 6 cycles (HR, 0.835; 95% CI, 0.572-1.221; P=0.35) or 8 cycles (HR, 0.972; 95% CI, 0.606-1.558; P=0.91) of chemotherapy did not show significantly prolonged DFS. However, the 3-year DFS and 5-year OS rates of patients who received 6 cycles of chemotherapy appeared to be superior to those of patients who received 4 and 8 cycles of chemotherapy. Conclusions: For patients with stage III GC, 4 to 6 cycles of XELOX or SOX chemotherapy may be a favorable option. This study provides a rationale for further randomized clinical trials.

Strain-induced islands and nanostructures shape transition's chronology on InAs (100) surface

Gambaryan, Karen M.,Aroutiounian, Vladimir M.,Simonyan, Arpine K.,Ai, Yuanfei,Ashalley, Eric,Wang, Zhiming M. Techno-Press 2014 Advances in nano research Vol.2 No.4

The self-assembled strain-induced sub-micrometric islands and nanostructures are grown from In-As-Sb-P quaternary liquid phase on InAs (100) substrates in Stranski-Krastanow growth mode. Two samples are under consideration. The first sample consists of unencapsulated islands and lens-shape quantum dots (QDs) grown from expressly inhomogeneous liquid phase. The second sample is an n-InAs/p-InAsSbP heterostructure with QDs embedded in the p-n junction interface. The morphology, size and shape of the structures are investigated by high-resolution scanning electron (SEM) and transmission electron (TEM) microscopy. It is shown that islands, as they decrease in size, undergo shape transitions. Particularly, as the volume decreases, the following succession of shape transitions are detected: sub-micrometric truncated pyramid, {111} facetted pyramid, {111} and partially {105} facetted pyramid, completely unfacetted "pre-pyramid", hemisphere, lens-shaped QD, which then evolves again to nano-pyramid. A critical size of $5{\pm}2nm$ for the shape transformation of InAsSbP-based lens-shaped QD to nano-pyramid is experimentally measured and theoretically evaluated.