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Effect of digestion product of royal jelly protein on SGC-7901 gastric cancer cell
Fu Li,Wang Tianshi,Song Jianxin 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1
Royal jelly used for larvae and queens has many health-promoting properties such as spatial memory improvement, antibacterial activity and antioxidant capacity. However, the anticancer ability of the royal jelly is not unknown. In this study, effect of the royal jelly protein on SGC-7901 gastric cancer cell was investigated, and the key factors including the cell morphological, the colony formation, the proliferation, the cycle, and the expression of p53 and poly (ADP-ribose) polymerase-1 (PARP-1) proteins were analyzed. Result showed royal jelly protein was excellent in inhibiting the growth of SGC-7901gastric cancer cell. After treating with the digestion product of royal jelly protein (0.05 mg/mL~0.20 mg/mL), the morphological of gastric cancer cell significantly shrink, and both of density and quantity (1000~491) of gastric cancer cell significantly (p < 0.05) decreased. The proliferation of gastric cancer was strongest inhibited by 62.84 % of 0.20 mg/mL royal jelly protein. The expression of p53 and PARP-1 protein of gastric cancer cell was respectively enhanced (0.29~0.46) and reduced (0.51~0.42). Moreover, the higher content of royal jelly protein (0.05 mg/mL~ 0.2 mg/mL), the stronger inhibit ability of 45 SGC-7901 gastric cancer cell. In conclusion, royal jelly protein can be used as a potential food in adjunctive therapy for gastric cancer.
Effect of digestion product of royal jelly protein on SGC-7901 gastric cancer cell
Fu Li,Wang Tianshi,Song Jianxin 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1
Royal jelly used for larvae and queens has many health-promoting properties such as spatial memory improvement, antibacterial activity and antioxidant capacity. However, the anticancer ability of the royal jelly is not unknown. In this study, effect of the royal jelly protein on SGC-7901 gastric cancer cell was investigated, and the key factors including the cell morphological, the colony formation, the proliferation, the cycle, and the expression of p53 and poly (ADP-ribose) polymerase-1 (PARP-1) proteins were analyzed. Result showed royal jelly protein was excellent in inhibiting the growth of SGC-7901gastric cancer cell. After treating with the digestion product of royal jelly protein (0.05 mg/mL~0.20 mg/mL), the morphological of gastric cancer cell significantly shrink, and both of density and quantity (1000~491) of gastric cancer cell significantly (p < 0.05) decreased. The proliferation of gastric cancer was strongest inhibited by 62.84 % of 0.20 mg/mL royal jelly protein. The expression of p53 and PARP-1 protein of gastric cancer cell was respectively enhanced (0.29~0.46) and reduced (0.51~0.42). Moreover, the higher content of royal jelly protein (0.05 mg/mL~ 0.2 mg/mL), the stronger inhibit ability of 45 SGC-7901 gastric cancer cell. In conclusion, royal jelly protein can be used as a potential food in adjunctive therapy for gastric cancer.
Glycogen Metabolism in Vibrio vulnificus Affected by malP and malQ
Han, Ah-Reum,Lee, Yeon-Ju,Wang, Tianshi,Kim, Jung-Wan The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.1
Vibrio vulnificus needs various responsive mechanisms to survive and transmit successfully in alternative niches of human and marine environments, and to ensure the acquisition of steady energy supply to facilitate such unique life style. The bacterium had genetic constitution very different from that of Escherichia coli regarding metabolism of glycogen, a major energy reserve. V. vulnificus accumulated more glycogen than other bacteria and at various levels according to culture medium and carbon source supplied in excess. Glycogen was accumulated to the highest level in Luria-Bertani (3.08 mg/mg protein) and heart infusion (4.30 mg/mg protein) complex media supplemented with 1% (w/v) maltodextrin at 3 h into the stationary phase. Regarding effect of carbon source, more glycogen was accumulated when maltodextrin (2.34 mg/mg protein) was added than when glucose or maltose (0.78.1-14 mg/mg protein) was added as an excessive carbon source to M9 minimal medium, suggesting that maltodextrin metabolism might affect glycogen metabolism very closely. These results were supported by the analysis using the malP (encoding a maltodextrin phosphorylase) and malQ (encoding a 4-${\alpha}$-glucanotransferase) mutants, which accumulated much less glycogen than wild type when either glucose or maltodextrin was supplied as an excessive carbon source, but at different levels (3.1-80.3% of wild type glycogen). Therefore, multiple pathways for glycogen metabolism were likely to function in V. vulnificus and that responding to maltodextrin might be more efficient in synthesizing glycogen. All of the glycogen samples from 3 V. vulnificus strains under various conditions showed a narrow side chain length distribution with short chains (G4-G6) as major ones. Not only the comparatively large accumulation volume but also the structure of glycogen in V. vulnificus, compared to other bacteria, may explain durability of the bacterium in external environment.
The evolved kurtogram: a novel repetitive transients extraction method for bearing fault diagnosis
Bin Pang,Yuzhi Hu,Heng Zhang,Bocheng Wang,Tianshi Cheng,Zhenli Xu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12
Kurtogram, a classic repetitive transients extraction method, plays an important role in bearing fault diagnosis. However, its performance is unstable since its index used for optimal sub-band component selection is sensitive to random pulse. Moreover, its sub-band component extraction is characterized by over-decomposition and under-decomposition defects. In this paper, an evolved Kurtogram (Evkurtogram) is proposed by designing a new index called the Gaussian distribution assigned Gini index (GDAG) for optimal sub-band component identification. In addition, a multi-scale empirical Fourier decomposition (MSEFD) for signal separation is proposed. GDAG is more suitable for quantifying the fault features of the signal due to its robustness of accidental pulses. MSEFD can achieve multi-scale decomposition of the signal reasonably and adaptively. The proposed Evkurtogram is compared with some relevant state-of-art algorithms by processing simulated and experimental bearing fault signals. It is demonstrated that the proposed Evkurtogram is effective and superior when compared to other approaches.
Glycogen Metabolism in Vibrio vulnificus Affected by malP and malQ
( Ah-reum Han ),( Yeon-ju Lee ),( Tianshi Wang ),( Jung-wan Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 한국미생물·생명공학회지 Vol.46 No.1
Vibrio vulnificus needs various responsive mechanisms to survive and transmit successfully in alternative niches of human and marine environments, and to ensure the acquisition of steady energy supply to facilitate such unique life style. The bacterium had genetic constitution very different from that of Escherichia coli regarding metabolism of glycogen, a major energy reserve. V. vulnificus accumulated more glycogen than other bacteria and at various levels according to culture medium and carbon source supplied in excess. Glycogen was accumulated to the highest level in Luria-Bertani (3.08 mg/mg protein) and heart infusion (4.30 mg/mg protein) complex media supplemented with 1% (w/v) maltodextrin at 3 h into the stationary phase. Regarding effect of carbon source, more glycogen was accumulated when maltodextrin (2.34 mg/mg protein) was added than when glucose or maltose (0.78-1.14 mg/mg protein) was added as an excessive carbon source to M9 minimal medium, suggesting that maltodextrin metabolism might affect glycogen metabolism very closely. These results were supported by the analysis using the malP (encoding a maltodextrin phosphorylase) and malQ (encoding a 4-α-glucanotransferase) mutants, which accumulated much less glycogen than wild type when either glucose or maltodextrin was supplied as an excessive carbon source, but at different levels (3.1-80.3% of wild type glycogen). Therefore, multiple pathways for glycogen metabolism were likely to function in V. vulnificus and that responding to maltodextrin might be more efficient in synthesizing glycogen. All of the glycogen samples from 3 V. vulnificus strains under various conditions showed a narrow side chain length distribution with short chains (G4-G6) as major ones. Not only the comparatively large accumulation volume but also the structure of glycogen in V. vulnificus, compared to other bacteria, may explain durability of the bacterium in external environment.