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RISS 인기검색어
- 검색키워드
- 저자 : W.S. Hancock (검색결과 5 건)
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The Use of Plasma Glycoproteomics for the Early Detection of Disease
W.S. Hancock,M. Hincapie,M. Kullolli,T. Plavina 한국당과학회 2008 한국당과학회 학술대회 Vol.2008 No.1
Mechanisms underlying disease pathogenesis are not well understood in the context of common etiological factors such as microbial infection, inflammation, malignancy or tissue breakdown. Such processes may be elucidated by identifying disease-related molecular markers, such as acute phase proteins, cytokines, cytoskeletal fragments and autoantigens. In an attempt to identify such markers, we have developed a novel platform, namely multiple lectin affinity chromatography (M-LAC) to study the plasma glycoproteome in patients and healthy donors in a series of studies ranging from cancer to autoimmune disease. The M-LAC platform allowed the evaluation of changes in concentration of glycoproteins, and to comprehensively survey the plasma proteome. We then used a second method, intact peptidomics, to assess changes in endogenous proteolytic activity by analyzing the low molecular weight (LMW) component of blood. The integrated proteomic and peptidomic analysis of plasma samples identified a number of cytoskeletal and Ca2+-binding proteins and their proteolytic fragments in the disease samples. The measurements were compared to healthy donors and several of the observed differential quantitations were independently verified by ELISA. The identified changes in plasma proteome and peptidome, and the underlying changes in glycosylation together with altered endogenous protease activity may result in the generation of novel autoantigens. We and others have confirmed this hypothesis by the observation of autoantibodies in patients and upon extension of these studies to larger populations of patient; we may gain additional understanding of the role of etiological factors in different disease pathways.
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Evidence from the northwestern Venezuelan Andes for extraterrestrial impact: The black mat enigma
Mahaney, W.C.,Kalm, V.,Krinsley, D.H.,Tricart, P.,Schwartz, S.,Dohm, J.,Kim, K.J.,Kapran, B.,Milner, M.W.,Beukens, R.,Boccia, S.,Hancock, R.G.V.,Hart, K.M.,Kelleher, B. Elsevier 2010 Geomorphology Vol.116 No.1
<P><B>Abstract</B></P><P>A carbon-rich black layer encrusted on a sandy pebbly bed of outwash in the northern Venezuelan Andes, previously considered the result of an alpine grass fire, is now recognized as a ‘black mat’ candidate correlative with Clovis Age sites in North America, falling within the range of ‘black mat’ dated sites (~12.9ka cal BP). As such, the bed at site MUM7B, which dates to <11.8ka <SUP>14</SUP>C years BP (raw dates) and appears to be contemporaneous with the Younger Dryas (YD) cooling event, marks a possibly much more extensive occurrence than previously identified. No fossils (megafauna) or tool assemblages were observed at this newly identified candidate site (3800a.m.s.l.), as in the case of the North American sites. Here, evidence is presented for an extraterrestrial impact event at ~12.9ka. The impact-related Andean bed, located ~20cm above 13.7–13.3ka cal BP alluvial and glaciolacustrine deposits, falls within the sediment characteristics and age range of ‘black mat’ dated sites (~12.9ka cal BP) in North America. Site sediment characteristics include: carbon, glassy spherules, magnetic microspherules, carbon mat ‘welded’ onto coarse granular material, occasional presence of platinum group metals (Rh and Ru), planar deformation features (pdfs) in fine silt-size fragmental grains of quartz, as well as orthoclase, and monazite (with an abundance of Rare Earth Elements—REEs). If the candidate site is ‘black mat’, correlative with the ‘black mat’ sites of North America, such an extensive occurrence may support the hypothesized airburst/impact over the Laurentide Glacier, which led to a reversal of Allerød warming and the onset of YD cooling and readvance of glaciers. While this finding does not confirm such, it merits further investigation, which includes the reconnaissance for additional sites in South America. Furthermore, if confirmed, such an extensive occurrence may corroborate an impact origin.</P>
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Effects of Amylase and Cellulase Supplementation in Sorghum-based Diets for Finishing Pigs
Park, J.S.,Kim, I.H.,Hancock, J.D.,Hines, R.H.,Cobb, C.,Cao, H.,Hong, J.W.,Kwon, O.S. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.1
Three experiments were conducted to determine the effects of a sorghum-specific enzyme system, derived from an Aspergillus niger and Bacillus subtilis fermentation extract (carbohydrase activity of 1,650 $\alpha$-amylase units and cellulase activity of 30 fibrinolytic units/mL), on growth performance of finishing pigs. In Exp. 1,192 pigs (average initial BW of 46.1 kg) were fed sorghum-based diets without or with 360 mL of enzyme system per ton of sorghum in a 78 d growth assay. For d 0 to 39, gain/feed was improved (p<0.03) with enzyme supplementation, but ADG was not affected (p>0.15). For d 39 to 78 and overall (d 0 to 78), ADG, gain/feed, and digestibilities of DM and N were not affected (p>0.13) by enzyme supplementation. Backfat thickness, fat-free lean index, and scores for stomach keratinization and ulcers also were not affected (p>0.15) by the dietary treatments. In Exp. 2,168 pigs (average initial BW of 58.4 kg) were fed diets without or with 150, 300, or 450 mL/ton of the same enzyme system used in Exp. 1. Adding as much as 450 mL enzyme system / ton of sorghum did not affect (p>0.15) ADG or gain/feed for d 0 to 29 of the growth assay. However, during d 29 to 63, ADG increased by 11% (linear effect, p<0.02) and gain/feed increased by 10% (linear effect, p<0.06) as enzyme concentration was increased from none to 450 mL/ton of sorghum. For the overall period (d 0 to 63), ADG tended to increase (p<0.08) with enzyme supplementation, but gain/feed and digestibilities of DM and N were not affected (p>0.14). Carcass characteristics (dressing percentage, backfat thickness, and fat free lean index) also were not affected (p>0.20) by addition of the enzyme system. In Exp. 3,176 pigs (average initial BW of 46.7 kg) were fed diets without or with 450, 900, or 1,350 mL/ton of the same enzyme system used in Exp. 1 and 2 in a 71 d growth assay. Adding up to 1,350 mL/ton of enzyme had no effects (p>0.15) on ADG, gain/feed, digestibilities of DM and N, and carcass characteristics (dressing percentage, backfat thickness, and fat-free lean index). In conclusion, finishing pigs fed diets with a sorghum-specific enzyme system showed some positive trends for improved growth performance, but those effects were not large and (or) consistent.
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