고등학교 복싱선수의 지구성 훈련이 혈당, 혈청지질 및 지단백의 변화에 미치는 영향

이윤관,이영웅,권판근,이한우 창원대학교 체육과학연구소 2002 경남 체육연구 Vol.7 No.2

트레이닝에 따른 인체의 반응 및 적응성 변화 양상은 운동의 강도, 빈도, 형태 및 기간에 따라서 달라지며, 피검자의 단련수준에 따라서도 달라진다. 이러한 변화 요인 중에서 혈청지질(Serum Lipid) 지단백(Lipoprotein)의 수준 변화는 심장계 질환 및 관상동맥 질환의 예방과 치료에 있어서 의학계는 물론 스포츠 과학분야에 있어서도 매우 많은 관심을 갖고 있으며, 이에 대한 연구가 활발하게 진행되고 있다. 유산소적 운동은 혈청지질 및 지단백, 그리고 혈당에 미치는 영향이 크다는 견해이지만 실제 연습방법에 따른 이러한 변화의 특성에 관한 보고는 거의 없는 실정이다. 따라서 본 연구자는 질병 예방과 운동선수의 훈련 효과를 높이기 위해 복싱선수들을 대상으로 하여 연습방법을 달리한 지구성 훈련을 시켜 혈청지질 및 지단백 그리고 혈당의 변화를 비교, 분석하여 운동선수들의 연습방법에 도움을 줄 수 있는 자료를 제공하고자 한다. 집중적 연습과 분산적 연습에 따른 유산소 지구성 운동을 13주 4일간 실시한 후 혈당, 혈청지질 및 지단백의 변화에 미치는 영향을 알아보기 위해 복싱선수 15명을 대상으로 Total-Cholesterol, HDL-Cholesterol, Triglyceride, Total-protein 및 glucose를 측정하여 비교 분석한 결과 다음과 같은 결론을 얻었다. 1.Total-Cholesterol은 훈련전 보다 13주 4일간 훈련후 모든 집단에서 증가하였으며, 집단간에 유의한(p<.05) 차이를 나타내었다. 2.HDL-Cholesterol은 훈련전 보다 13주 4일간 훈련후 집중적 연습집단에서는 증가하였으며, 집단간 유의한(p<.05)차이를 나타내었다. 3.Triglyceride는 훈련전보다 13주 4일간 훈련후 모든 집단에서 감소하였으며, 집단간 매우 유의한(p<.001) 차이를 나타내었다. 4.Total-Protein은 훈련전 보다 13주 4일간 훈련후 모든 집단에서 증가하였으며, 집단간 매우 유의한(p<.001) 차이를 나타내었다. 5.Glucose는 훈련전보다 13주 4일간 훈련후 모든 집단에서 감소하였으며, 집단간 유의한(p<.05) 차이를 나타내었다.

산업대학 건축공학과 교육과정 개발에 관한 연구

임응찬,한인웅,이승준,박재호,조방현,서형수,정구용,한기원,김진원,임상규,이재윤 三陟大學校 1996 論文集 Vol.29 No.2

By impproving the educational program of the Department of Architectural Engineering and studying the theory of learning and the applied method necessary for the development of nation and society, we should develop a satisfying course of education to meet the demand and skill in the industralized society for the purpose of cultivating men of baility with great personalities and creative minds. Therefore, this study focused on classifying several kinds of occupations in the prart of architectured related to what graduates from the department of Architectural Engineering go with into the society. Accordingly after choosing some works adequate to each occupation by the proposed curricula, we tried to establish the scope of education and courses corresponding to the jobs and thus and develop a satisfying curriculum.

Structural and expression analysis of prolamin genes in Oryza sativa L.

Ung-Han Yoon,Tae-Ho Kim,Jeonghwa Lee,JangHo Hahn,Yeon-Ki Kim,Gang-Seob Lee,Hyeon-So Ji,ChangKug Kim,문정환,Young Mi Kim 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.3

Rice is a staple crop with a small genome of 389 Mb. Rice grain is a source of carbohydrates and proteins and has a relatively low protein content compared to other legume seeds. Glutelin and prolamin are the major storage proteins in rice. Prolamins are characterized by high glutamine and proline content and are generally soluble only in strong alcohol solutions. In this study, we obtained a total of 51,383 expressed sequence tags (ESTs)from Ilpumbyeo (Oryza sativa L.), of which 33,201 and 18,182 clones were obtained from immature and germinating seeds, respectively. From the EST clones, 15,148unigenes were identified, and 2,590 genes were expressed in both immature and germinating seeds. Gene expression profiling of rice prolamins indicated that prolamin gene expression increased 5 days after heading and reached maximal expression after 30 days, suggesting a high demand for prolamins during seed development and germination. Phylogenetic analysis grouped 33 prolamin genes based on the abundance of sulfur-containing amino acids methionine and cysteine according to the deduced amino acid sequences. Our results enhance the understanding of the regulation of seed maturation and germination, which can result in improved agricultural traits for the seed industry.

연구논문 : 자연과학 ; 벼 Ac,Ds 삽입 변이체집단의 표현형 분석을 통한 유전자기능 연구

윤웅한 ( Ung Han Yoon ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.1

Rice is one of the world`s most important crops, particularly in Asia, for human consumptions. Rice consumption has been increased due to versatility of nutrients and tastes. In addition, Rice has taken a role of model plant since the small size of the genome was completely sequenced (Jun et al., 2002, Feng et al., 2002). To increase higher yield potentials of crops, the discovery of novel genes and the construction of QTL maps should be essential projects in genomic researches. This study has been carried out to construct the database for the insertional mutant population generated by Ac/Ds transposable element system. In addition, the biological functions of genes useful for agriculture have been studied and the possibility to create new varieties through the biotechnological method have been exploited. The data and information obtained through this study could be used as a basis for intellectual property and be helpful for breeding to select useful gene as analyzing gene function analysis. This project is performed to develop internationally competitive scale of insertional mutagenized population, and to construct databases of molecular information on Ds insertion sites. Ultimate goals are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes.

일본의 식물유전체 연구현황 및 전망

윤웅한 ( Ung Han Yoon ),이정화 ( Jeong Hwa Lee ),이강섭 ( Gang Seob Lee ),김영미 ( Young Mi Kim ),지현소 ( Hyeon So Ji ),김태호 ( Tae Ho Kim ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.5

In Japan, plant genomics research is mainly leaded by the national research institutes. The various structural studies such as rice genome has allowed researchers to analyze useful traits, and to focus their commercialization. With aims to facilitate structural and functional study in plant genome, NIAS (National Institute of Agrobiological Sciences) constructed Poaceae genome DB and RIKEN (Rikagaku Kenkyusho) built DB for Arabidopsis genome and plant full-length cDNA. NIG (National Institute of Genetics) constructed a national biological resources DB (National Bio Resource Project). This compiling DB provides a variety of genome-related research materials for researchers in the field. Recently, as an effort to resolve global issues of food supply and environmental problems, New Agri-genome Project has been performed aiming to develop an innovative agricultural technologies for the quantity, disease resistance and identifying useful genes related to environmental problems. In addition, in order to improve agricultural productivity in developing countries, JIRCAS assisted technical supports for the plant genome research and developed NERICA rice, which is suitable for African area. Such these approaches are expected to contribute to solving the global issues about food, energy and environment in the world.

연구보문 : 자연과학 ; 자포니카 벼의 종자 저장단백질 유전자 구조 및 발현분석 연구 현황

윤웅한 ( Ung Han Yoon ),이정화 ( Jeong Hwa Lee ),이강섭 ( Gang Seob Lee ),김영미 ( Young Mi Kim ),한장호 ( Jang Ho Hahn ),지현소 ( Hyeon So Ji ),윤성원 ( Sung Won Yoon ),이종렬 ( Jong Yeol Lee ),김태호 ( Tae Ho Kim ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.3

Based on the outcome of the rice genome sequencing, a lot of researches for identification of genes underlying useful traits and their utilization are being carried out recently. Especially, rice seed storage proteins are used as a main nutrition source for human while they are used as the nitrogen source for plant growth during seed germination. Rice seed storage proteins are divided by solubility in solvent into weak acid and alkali soluble glutelin, alcohol soluble prolamin, and salt soluble globulin. The rice genome contains 11 glutelin genes and 33 prolamin genes. In the case of prolamin genes, 15 genes are located in 15 Mb region on the chromosome 5. Clustering of seed storage protein genes on the same chromosomal region indicates that they are evolved from the same chromosome or they share similar gene expression regulatory region. Only single globulin gene existed on the chromosome 5. As a result of tissue- specific expression pattern analysis of seed storage protein genes using microarray, glutelin and most of prolamin genes were expressed only in seed endosperm, while a few prolamin and the globulin genes are expressed in seed endosperm and germinating seed. As a result of isolating promoter regions of the seed storage protein genes and analyzing their expression pattern in seed, glutelin type C1 promoter showed expression in whole seed, and glutelin type B5 and α-globulin promoter showed strong expression. This paper reviewed on the structure and expression profile of rice seed storage protein genes, and will provide information on the recent research status to the researchers who are studying on the improvement of seed traits and production of substances.

Identification of genetic diversity and DNA polymorphisms in Korean rice accessions through resequencing

In-Seon Jeong,Ung-Han Yoon,Gang-Seob Lee,Hyeon-So Ji,Hyun-Ju Lee,Tae-Ho Kim 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

With the rapid development of sequencing technologies, next-generation sequencing is widely utilized for molecular breeding in several crops including rice. We performed whole genome resequencing of ten Korean rice accessions including six cultivars and four mutant lines. In total, 2,448 million raw reads were generated with over 58x coverage of Nipponbare genome. We mapped the reads from each of the ten accessions onto genomic sequence of japonica rice cultivar, Nipponbare. We detected 3,144,016 SNPs, which estimated to be one per 2.2kb on average. We found SNPs in genes that have been reported to be involved in rice flowering time regulation and bacterial blight resistance among ten rice accessions. Unmapped region against Nipponbare genome occupied about 1 ~ 2% in each accession. Over 50% of the unmapped region were found in the repeat region. The minimum length of gap in all accessions were 1bp and the maximum length of gap was 45,967bp in Ilpum. We also identified 3,497 possible gene loss events within these unmapped regions. The frequency of gene loss in each chromosome ranged from 33 on chromosome 5 to 913 on chromosome 11. The genetic variations we detected among ten rice accessions will provide invaluable resources for identification of genes associated with diverse traits of agronomical importance for molecular breeding.

Wound-induced expression of the OsDof1 gene promoter in the Ds insertion mutant and transgenic plants

Hyemin Lim,Sung Han Park,A-Ram Kim,Hyeon-So Ji,Ung-Han Yoon,Tae-Ho Kim,Soo-Chul Park,Jang-Ho Hahn,Gang-Seob Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

In plants, the Dof (DNA binding with One Finger) proteins are plant-specific transcription factors with a particular class of zinc-finger DNA-binding domain. The Dof genes have been predicted 30 different Dof genes in the rice Oryza sativa genome by phylogenetic analysis. The mostly Dof proteins contain a conserved region of 50 amino acids with a C2-C2 zinc finger motifs that binds a cis-regulatory element sequence 5’-T/AAAAG-3’. We found that a member of the DOF transcription factor family, Dof1 gene of rice, was expressed to wound from Ds insertion mutant population. Sequencing of the flanking regions of the transposon insertion site indicated that the gene-trap had been inserted near the front of the second exon of OsDof1 gene in chromosome 7. Genomic southern analysis revealed that mutant line contained a single copy of Ds gene trap. The Ds tagged rice mutant line, OsDof1::Ds, wound-inducible GUS expression was identified. To analyze the cis-acting elements, we constructed fusion genes with the OsDof1 promoter fused to the β-glucuronidase (GUS) reporter gene and transformed Arabidopsis and rice plants with these constructs. Wound-induced GUS expression was observed in the leaves of transgenic OsDof1::GUS rice and Arabidospsis plants. These results showed that, OsDof1 protein might be involved in stress responses and growth regulation in plant, might plays a role as a transcription regulator in stress response signal transduction pathways of plant.

Exploring Rice DNA Polymorphisms by Whole-Genome Resequencing

In-Seon Jeong,Ung-Han Yoon,Gang-Seob Lee,Hyeon-So Ji,Hyun-Ju Lee,Tae-Ho Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Progress in next-generation sequencing technologies have enabled discovery of massive amount of genome-wide DNA polymorphisms, single nucleotide polymorphisms (SNPs) and insertion-deletion (InDels), which are an invaluable resource to analyze genetic diversity in a population. We performed whole-genome resequencing of ten Korean rice accessions including six cultivars and four mutant lines. A total of 2,448 million raw reads was generated with 58-fold coverage and uniquely mapped to 87.5% of the Nipponbare as a reference genome. We identified 3,240,025 DNA polymorphisms including 2,867,878 SNPs, 151,845 insertions and 220,302 deletions between the Korean rice accessions and Nipponbare. We observed that in ten Korean rice accessions, the frequency of potential SNPs was estimated to be one per 2.1kb on Nipponbare (382Mb). According to annotation of DNA polymorphisms, 634,617 SNPs were found in gene region, and only 169,738 SNPs were occurred in coding region. Altogether, 86,251 non-synonymous SNPs were located on 76,891 genes. We also examined the cultivar-specific SNPs to select candidate SNPs which would have possibility of being associated with unique phenotype or agronomical trait of each cultivar. It was estimated that the portion of cultivar specific SNPs is 1~12% of the total SNPs. These DNA polymorphisms obtained from our result will provide an invaluable resource to identify molecular markers and genes associated with diverse traits of agronomical importance.

Identification of genetic polymorphisms in Korean rice accessions by Whole-genome sequencing

In-Seon Jeong,Ung-Han Yoon,Gang-Seob Lee,Hyeon-So Ji,Hyun-Ju Lee,Tae-Ho Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Advances in genome sequencing technologies have aided discovery of millions of genome-wide DNA polymorphisms, single nucleotide polymorphisms (SNPs) and insertion-deletion (InDels), which are an invaluable resource to analyze genetic diversity in a population. We performed whole-genome resequencing of ten Korean rice accessions including six cultivars and four mutant lines. A total of 2,447 million raw reads were generated with over 58x coverage and detected 3,240,025 DNA polymorphisms between the Korean rice accessions and Nipponbare as reference genome. We observed that in ten Korean rice accessions, the frequency of potential SNPs was estimated to be one per 2.1kb on Nipponbare (382Mb). Potential SNPs were classified into two types, homozygous SNP and heterozygous SNP, which approximately 87% of the total was homozygous SNPs from ten accessions and heterozygous SNPs accounted for 13%. According to annotation of DNA polymorphisms, 634,620 SNPs were found in gene region, and only 169,738 SNPs were occurred in coding region. Altogether, 86,251 non-synonymous SNPs were located on 76,891 genes. We also examined genes which had at least one SNP in all ten accessions. It was estimated that the total of 290 genes had one or more non-synonymous SNPs and 25 genes had only synonymous SNPs. These genes were functionally classified based on gene ontology (GO). These DNA polymorphisms obtained from our result will provide an invaluable resource to identify molecular markers and genes associated with diverse traits of agronomical importance.