Laryngopharyngeal Reflux in Children with Chronic Otitis Media with Effusion

( Anna Gorecka-tuteja ),( Izabela Jastrzebska ),( Jacek Skiadzien ),( Krzysztof Fyderek ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2016 Journal of Neurogastroenterology and Motility (JNM Vol.22 No.3

Background/Aims To evaluate the characteristic properties of laryngopharyngeal reflux (LPR) and gastroesophageal reflux (GER) in children with otitis media with effusion (OME) using 24-hour multichannel intraluminal impedance combined with dual-probe (pharyngeal and esophageal) pH-metry. Methods Children aged 7-10 years of age with OME underwent 24-hour multichannel intraluminal impedance pH-metry. The upper pH sensor was situated 1 cm above the upper esophageal sphincter, and the lower pH sensor was placed 3-5 cm above the lower esophageal sphincter. Parents were asked to complete the gastroesophageal reflux assessment of symptoms in a pediatrics questionnaire. Results Twenty-eight children were enrolled; LPR was detected in 19 (67.9%) children. The criteria of the LPR diagnosis was the presence of at least one supraesophageal episode with a pH < 5.0 and a change in the pH value measured from the initial level at the upper sensor of > 0.2. In total, 64 episodes were observed. Assessment of all LPR episodes showed the presence of 246 episodes in the entire study. A considerable predominance of weakly acidic episodes (87.8%) was noted; there were 6.5% acidic episodes, and weakly alkaline episodes reached 5.7%. Pathological GER was noted in 10 (35.7%) subjects. Acid GER was detected in 8 children, 2 of whom demonstrated non-acidic reflux. In the LPR-negative patients, no pathological GER was confirmed with the exception of a single case of non-acidic reflux. Conclusions LPR was frequently noted in the group of children with OME, and it might be an important risk factor in this common disease. (J Neurogastroenterol Motil 2016;22:452-458)

A novel approach for amine derivatization of MoS₂ nanosheets and their application toward label-free immunosensor

Kukkar, Manil,Tuteja, Satish K.,Kumar, Parveen,Kim, Ki-Hyun,Bhadwal, Akhshay Singh,Deep, Akash Elsevier 2018 Analytical Biochemistry Vol.555 No.-

<P><B>Abstract</B></P> <P>The application of molybdenum disulfide (MoS<SUB>2</SUB>) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS<SUB>2</SUB> nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS<SUB>2</SUB> nanosheets. This newly proposed approach helped to immobilize the MoS<SUB>2</SUB> nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS<SUB>2</SUB> nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS<SUB>2</SUB> nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the ‘prostate specific antigen’. The application of the proposed immunosensor was characterized with a low detection limit (10<SUP>−3</SUP> ng/mL) over a very wide quantitation range (10<SUP>−3</SUP> to 200 ng/mL).</P> <P><B>Highlights</B></P> <P> <UL> <LI> The bioconjugation of MoS<SUB>2</SUB> nanosheets has been processed with a novel strategy. </LI> <LI> MoS<SUB>2</SUB> nanosheets were exfoliated and electrochemically modified with 2-aminobenzylamine. </LI> <LI> MoS<SUB>2</SUB> nanosheets have been interfaced with antibodies via facile EDC/NHS cross-linking chemistry. </LI> <LI> MoS<SUB>2</SUB> nanosheets immunosensor for PSA has offered a low detection limit and a very wide range of analysis. </LI> </UL> </P>

A New Electrolytic Synthesis Method for Few-Layered MoS₂ Nanosheets and Their Robust Biointerfacing with Reduced Antibodies

Kukkar, Manil,Tuteja, Satish K.,Sharma, Amit L.,Kumar, Vinod,Paul, Ashok K.,Kim, Ki-Hyun,Sabherwal, Priyanka,Deep, Akash American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.26

<P>We report an efficient method for the synthesis of few-layered MoS2 nanosheets and demonstrate their application in the label-free detection of the prostate-specific antigen (PSA) cancer marker. As a novel strategy, the electro-dissolution of molybdenum metal sheets in the presence of Na+ and S2- ions led to the formation of Na+ intercalated MoS2. Further exfoliation by ultrasonication yielded the desired formation of few-layered MoS2 nanosheets. After comprehensive characterization, the synthesized MoS2 nanosheets were channeled in a field-effect transistor (FET) microdevice. Chemically reduced anti-PSA antibodies were immobilized on the MoS2 channel above the FET microdevice to construct a specific PSA immunosensor. The antibodies were deliberately reduced to expose the hinge-region disulfide bonds. This approach offered a robust and site-directed immunosensing device through biointerfacing of the sulfhydryl groups (SH) in the reduced antibody with the surface S atoms of MoS2. This device was validated as an effective immunosensor with a low detection limit (10(-5)ng/mL) over a wide linear detection range (10(-5) to 75 ng/mL).</P>

A Control Policy for a Single Sever Queue

Kumar, Ashok,Tuteja, R. K. 한국경영과학회 1984 한국경영과학회지 Vol.9 No.1

This paper deals with Random Idle Policy for a single server queue. If the system is in operation state, server will serve the units as long as there is any unit in the system and server will go to idle state for a random period as the system become empty. Two systems are studied as follows (ⅰ) when the server does not wait on return to empty queue and goes to idle state again of the same distribution random period and (ⅱ) when the server waits for an arrival on return to empty queue. For the first system idle period is taken exponentially distributed and for the second, it is taken with p.d.f. D(χ). Applying linear cost structure, the optimal value of the mean duration of idle period has been obtained. At the end, a comparison is made between these systems.

Detection of Virulence-Associated Genes in Clinical Isolates of Bacillus anthracis by Multiplex PCR and DNA Probes

Sanjay Kumar,Urmil Tuteja 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.11

Rapid Multiplex PCR Assay for the Simultaneous Detection of the Brucella Genus, B. abortus, B. melitensis, and B. suis

( Kumar Sanjay ),( Urmil Tuteja ),( Kumari Sarika ),( Dhirendra Kumar Singh ),( Ashok Kumar ),( Om Kumar ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.1

The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.

Functional Roles of Ca _v 1.3(α _1D ) Calcium Channels in Atria : Insights Gained From Gene-Targeted Null Mutant Mice

Zhang, Zhao,He, Yuxia,Tuteja, Dipika,Xu, Danyan,Timofeyev, Valeriy,Zhang, Qian,Glatter, Kathryn A.,Xu, Yanfang,Shin, Hee-Sup,Low, Reginald,Chiamvimonvat, Nipavan Ovid Technologies Wolters Kluwer -American Heart A 2005 CIRCULATION - Vol.112 No.13

<P>Previous data suggest that L-type Ca2+ channels containing the Cav1.3(alpha(1D)) subunit are expressed mainly in neurons and neuroendocrine cells, whereas those containing the Cav1.2(alpha1C) subunit are found in the brain, vascular smooth muscle, and cardiac tissue. However, our previous report as well as others have shown that Cav1.3 Ca2+ channel-deficient mice (Cav1.3(-/-)) demonstrate sinus bradycardia with a prolonged PR interval. In the present study, we extended our study to examine the role of the Cav1.3(alpha1D) Ca2+ channel in the atria of Cav1.3(-/-) mice.</P>

Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India

Joseph J. Kingston,Kuruvilla Zachariah,Urmil Tuteja,Sanjay Kumar,Harsh Vardhan Batra 한국미생물학회 2009 The journal of microbiology Vol.47 No.1

Vibrio cholerae isolates recovered from cholera outbreaks in Bhind district of Madhya Pradesh and Delhi, Northern India were characterized. The O1 serogroup isolates from Bhind outbreak were of Inaba serotype whereas both Ogawa and Inaba serotypes were recovered from Delhi. PCR analysis revealed that only O1 serogroup V. cholerae isolates carried the virulence-associated genes like ctxA, tcpA, ace, and zot. Molecular typing by repetitive sequence based ERIC, VCR1, and VC1 PCR’s revealed similar DNA profile for both Inaba and Ogawa serotypes. A discrete VC1-PCR band identified among the El Tor strains had greater similarity (>97%) to the V. cholerae genome sequence and therefore has the potential to be used as a marker for the identification of the V. cholerae strains. Non-O1 strains recovered from Bhind region differed among themselves as well as from that of the O1 isolates. All the O1 serogroup isolates possessed SXT element and were uniformly resistant to the antibiotics nalidixic acid, polymyxin-B, furazolidone, cloxacilin, trimethoprim-sulfamethaxazole, and vibriostatic agent O129. Inaba strains from both Delhi and Bhind differed from Ogawa strains by their resistance to streptomycin despite sharing similar DNA patterns in all the three rep-PCRs. Though Delhi and Bhind are separate geographical regions in Northern India, Inaba strains from both these places appear to be closely related owing to their similarity in antibiogram and genetic profile.

A comprehensive review on nano-molybdenum disulfide/DNA interfaces as emerging biosensing platforms

Kukkar, Manil,Mohanta, Girish C.,Tuteja, Satish K.,Kumar, Parveen,Bhadwal, Akhshay Singh,Samaddar, Pallabi,Kim, Ki-Hyun,Deep, Akash Elsevier 2018 Biosensors & Bioelectronics Vol.107 No.-

<P><B>Abstract</B></P> <P>The development of nucleic acid-based portable platforms for the real-time analysis of diseases has attracted considerable scientific and commercial interest. Recently, 2D layered molybdenum sulfide (2D MoS<SUB>2</SUB> from here on) nanosheets have shown great potential for the development of next-generation platforms for efficient signal transduction. Through combination with DNA as a biorecognition medium, MoS<SUB>2</SUB> nanostructures have opened new opportunities to design and construct highly sensitive, specific, and commercially viable sensing devices. The use of specific short ssDNA sequences like aptamers has been proven to bind well with the unique transduction properties of 2D MoS<SUB>2</SUB> nanosheets to realize aptasensing devices. Such sensors can be operated on the principles of fluorescence, electro-cheumuluminescence, and electrochemistry with many advantageous features (e.g., robust biointerfacing through various conjugation chemistries, facile sensor assembly, high stability with regard to temperature/pH, and high affinity to target). This review encompasses the state of the art information on various design tactics and working principles of MoS<SUB>2</SUB>/DNA sensor technology which is emerging as one of the most sought-after and valuable fields with the advent of nucleic acid inspired devices. To help achieve a new milestone in biosensing applications, great potential of this emerging technique is described further with regard to sensitivity, specificity, operational convenience, and versatility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In this review, an outlook of 2-D nanomaterial inspired biosensors is described. </LI> <LI> This article focused on MoS<SUB>2</SUB>-DNA/aptamer biosensors and their practical applications. </LI> <LI> 2-D MoS<SUB>2</SUB>-DNA interfaces are recognized as the next-generation biosensors. </LI> </UL> </P>

Graphene quantum dot modified screen printed immunosensor for the determination of parathion

Mehta, J.,Bhardwaj, N.,Bhardwaj, S.K.,Tuteja, S.K.,Vinayak, P.,Paul, A.K.,Kim, K.H.,Deep, A. Academic Press 2017 Analytical biochemistry Vol.523 No.-

<P>The widespread use of pesticides has immense effect on increased crop productions. However, they are also responsible for posing detrimental health hazards and/or for contaminating the environment with chemical residues. A routine and an on-field detection of pesticide residues in different food, water, and soil samples has become a need of the hour for which biosensors can offer a viable alternative. The present work reports a functionalized graphene quantum dot (GQD) based screen printed electro-chemical immunosensor for the detection of parathion. The application of GQDs has permitted the realization of a sensitive, robust, and reproducible sensor unlike those carried out earlier for the similar purposes. This immunosensor exhibited a dynamic linear response for parathion within the range of 0.01 -10(6) ng/L with a very low detection limit of 46 pg/L. According to the analysis of potential interferences, the proposed sensor was specifically detecting parathion even in the presence of its metabolite, paraoxon. The investigations of the proposed sensing approach with respect to stability, response reproducibility, and regeneration have fully supported its potential practical applicability. (C) 2017 Elsevier Inc. All rights reserved.</P>