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( Kumar Sanjay ),( Urmil Tuteja ),( Kumari Sarika ),( Dhirendra Kumar Singh ),( Ashok Kumar ),( Om Kumar ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.1
The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratory-acquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.