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양영헌,Takao Raku,송은정,박성희,유동원,박형연,김병기,김형주,이상현,김형섭,Yutaka Tokiwa 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
To perform the lipase-catalyzed synthesis of Lascorbic acid derivatives from plant-based compounds such as cinnamic and ferulic acid under mild reaction conditions, the activities of immobilized Candida ntarctica lipase with different cinnamic acid esters and substituted cinnamic acids were compared. As a result, immobilized C. ntarctica lipase was found to prefer vinyl cinnamic acid to other esters such as allyl-, ethyl-, and isobutyl cinnamic acids as well as substituted cinnamic acids such as pcoumaric acid, caffeic acid, ferulic acid, and sinapic acid. Based on these results, large-scale synthesis of 6-Ocinnamyl-L-ascorbic acid ester was performed using immobilized C. ntarctica lipase in dry organic solvent,resulting in 68% yield (493 mg) as confirmed by 13CNMR.