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Husaini, Amjad M.,Aquil, Samina,Bhat, Mukhtar,Qadri, Tabassum,Kamaluddin, Kamaluddin,Abdin, Malik Zainul The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.2
A high-efficiency, reproducible somatic embryogenesis system for strawberry cultivar Chandler was developed. Thirty-one somatic embryos per explant(max no.) were recorded in leaf discs which were cultured on medium containing MS salts+$B_5$ vitamins+2% glucose+4.0 mg $1^{-1}$TDZ(Thidiazuron) and incubated at $10{\pm}1^{\circ}C$ under darkness for one week followed by three weeks under 16-h photoperiod. The scanning electron microscopic(SEM) ontogeny revealed the normal development of somatic embryos from globular to heart-shaped and dissection microscopy from torpedo-shaped to cotyledonary-stage embryos. The maximum germination percentage of 48% could be obtained on MS medium containing kinetin(1.0 mg $1^{-1}$) and the maximum survival percentage(79%) of plantlets after four weeks was found to be in the mixture of vermiculite, peatmoss, and soilrite(1:1:1).
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Amjad M. Husaini,Samina Aquil,Mukhtar Bhat,Tabassum Qadri,Kamaluddin,Malik Zainul Abdin 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.2
A high-efficiency, reproducible somatic embryogenesis system for strawberry cultivar Chandler was developed. Thirty-one somatic embryos per explant (max no.) were recorded in leaf discs which were cultured on medium containing MS salts + B5 vitamins + 2% glucose + 4.0 mg l-¹TDZ (Thidiazuron) and incubated at 10 ± 1 ℃ under darkness for one week followed by three weeks under 16-h photoperiod. The scanning electron microscopic (SEM) ontogeny revealed the normal development of somatic embryos from globular to heart-shaped and dissection microscopy from torpedo-shaped to cotyledonary-stage embryos. The maximum germination percentage of 48% could be obtained on MS medium containing kinetin (1.0 mg l-¹) and the maximum survival percentage (79%) of plantlets after four weeks was found to be in the mixture of vermiculite, peatmoss, and soilrite (1:1:1). A high-efficiency, reproducible somatic embryogenesis system for strawberry cultivar Chandler was developed. Thirty-one somatic embryos per explant (max no.) were recorded in leaf discs which were cultured on medium containing MS salts + B5 vitamins + 2% glucose + 4.0 mg l-¹TDZ (Thidiazuron) and incubated at 10 ± 1 ℃ under darkness for one week followed by three weeks under 16-h photoperiod. The scanning electron microscopic (SEM) ontogeny revealed the normal development of somatic embryos from globular to heart-shaped and dissection microscopy from torpedo-shaped to cotyledonary-stage embryos. The maximum germination percentage of 48% could be obtained on MS medium containing kinetin (1.0 mg l-¹) and the maximum survival percentage (79%) of plantlets after four weeks was found to be in the mixture of vermiculite, peatmoss, and soilrite (1:1:1).
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