상담 장면에서의 명리의 활용에 대한 국내 연구 동향 분석

홍성규 ( Sunggyu Hong ),곽희용 ( Hui-yong Kwak ),김종우 ( Jong-woo Kim ),정선용 ( Sun-yong Chung ) 대한한방신경정신과학회 2020 동의신경정신과학회지 Vol.31 No.4

Objectives: The aim of this study is to investigate current research trends of Four Pillars of Destiny and verify its values and potential in the counselling scene, as the Four Pillars of Destiny’s territory has been expanding to counselling, medical and psychiatric realm nowadays. Methods: The studies were searched from psychotherapy to general consultation, directly or indirectly related to counseling and Four Pillars of Destiny. Twenty-one published research studies were selected for analysis. The studies were categorized into 7 groups, meta-analysis, comparison with other personality tests, user’s trend analysis, utilization in job counseling, disease prediction study, utilization in treatment counseling, and use in Korean medicine. Results: The selected studies attempted to expand Four Pillars of Destiny’s usage through combination with other fields such as artificial intelligence, Korean medicine, and personality test. Furthermore by analyzing Four Pillars of Destiny itself to extract its key elements in counseling, such as therapeutic counseling factors and occupational counseling factors. Conclusions: At present, there are no standard use of Four Pillars of Destiny in counseling scene, for no large-scale research has been conducted or completed on this subject. This current status quo leads this paper to end up just understanding the counseling factors and possibilities of Four Pillars of Destiny rather than its psychological theory and clinical effect. However, this research trend analysis will be helpful in preparing future studies investigating Four Pillars of Destiny’s counseling effect, application in the counseling scene and its psychological theory. Also, further studies, including confirmation of the theory through the operational definition, prospective research, control study, statistical technique are required in order to evaluate Four Pillars of Destiny's psychological theory and its effects to verify its use in clinical scenes.

Photoaging protective effects of BIOGF1K, a compound-K-rich fraction prepared from Panax ginseng

Hong, Yo Han,Kim, Donghyun,Nam, Gibaeg,Yoo, Sulgi,Han, Sang Yun,Jeong, Seong-Gu,Kim, Eunji,Jeong, Deok,Yoon, Keejung,Kim, Sunggyu,Park, Junseong,Cho, Jae Youl The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.1

Background: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigated the effects of BIOGF1K on UVB-induced photoaging events. Methods: We analyzed the ability of BIOGF1K to prevent UVB-induced apoptosis, enhance matrix metalloproteinase (MMP) expression, upregulate anti-inflammatory activity, reduce sirtuin 1 expression, and melanin production using reverse transcription-polymerase chain reaction, melanin content assay, tyrosinase assay, and flow cytometry. We also evaluated the effects of BIOGF1K on the activator protein-1 signaling pathway, which plays an important role in photoaging, by immunoblot analysis and luciferase reporter gene assays. Results: Treatment of UVB-irradiated NIH3T3 fibroblasts with BIOGF1K prevented UVB-induced cell death, inhibited apoptosis, suppressed morphological changes, reduced melanin secretion, restored the levels of type I procollagen and sirtuin 1, and prevented mRNA upregulation of MMP-1, MMP-2, and cyclo-oxygenase-2; these effects all occurred in a dose-dependent manner. In addition, BIOGF1K markedly reduced activator-protein-1-mediated luciferase activity and decreased the activity of mitogen-activated protein kinases (extracellular response kinase, p38, and C-Jun N-terminal kinase). Conclusion: Our results strongly suggest that BIOGF1K has anti-photoaging activity and that BIOGF1K could be used in anti-aging cosmeceutical preparations.

띠형 강보강재에 설치된 수동저항부재의 설치 특성에 따른 상호간섭계수 평가

정성규(Sunggyu Jung),홍기권(Kikwon Hong),한중근(Jung-Geun Han),이광우(Kwang-Wu Lee) 한국지반신소재학회 2014 한국지반신소재학회 논문집 Vol.13 No.3

본 연구에서는 띠형 강보강재에 ‘?’ 형태의 수동저항부재를 설치하는 보강재에 대한 인발시험 결과를 바탕으로 수동저항부재의 간격 및 설치위치에 따른 간섭효과를 분석하였다. 수동저항부재에서 발현되는 최대수동저항력은 수동저항부재의 설치 위치에 따라 차이가 발생하였으며, 전면벽체에 근접하여 설치된 수동저항부재에서 더 큰 수동저항력이 발현되는 것을 확인하였다. 최대인발력을 사용하여 간섭계수(DI)를 평가한 결과, 지지부재 직경과 간격의 비(S/B)가 동일한 경우에는 수동저항부재의 설치 위치에 따른 인발력 차이를 반영하지 못하는 것으로 평가되었다. 그러나 최대수동저항력을 사용하여 간섭계수(F)를 산정할 경우에는 수동저항부재의 설치 간격뿐만 아니라, 수동저항부재의 설치 위치에 따른 최대수동저항력 이를 반영하는 것으로 분석되었다. This paper describes interference effect analysis of transverse member based on large-scale pullout test results of steel strip reinforcement with ‘(?)’ type transverse member. The maximum passive resistance has a difference according to the installed location of transverse member, and the total pullout resistance is increased, when transverse member was closed to the wall facing. The degree of interference confirmed that the install location of transverse member cannot reflect the pullout force differential, if is equal. However, The interference factor based on maximum passive resistance reflected the differential of maximum passive resistance and install location of transverse member.

Newly Identified AhR-Active Compounds in the Sediments of an Industrial Area Using Effect-Directed Analysis

Kim, Jaeseong,Hong, Seongjin,Cha, Jihyun,Lee, Junghyun,Kim, Taewoo,Lee, Sunggyu,Moon, Hyo-Bang,Shin, Kyung-Hoon,Hur, Jin,Lee, Jung-Suk,Giesy, John P.,Khim, Jong Seong American Chemical Society 2019 Environmental science & technology Vol.53 No.17

<P>Effect-directed analysis was used to identify previously unidentified aryl hydrocarbon receptor (AhR) agonists in sediments collected from a highly industrialized area of Ulsan Bay, Korea. The specific objectives were to (i) investigate potent fractions of sediment extracts using the H4IIE-<I>luc</I> bioassay, (ii) determine the concentrations of known AhR agonists (polycyclic aromatic hydrocarbons (PAHs) and styrene oligomers (SOs)), (iii) identify previously unreported AhR agonists in fractions by use of GC-QTOFMS, and (iv) evaluate contributions of individual compounds to overall AhR-mediated potencies, found primarily in fractions containing aromatics with log <I>K</I><SUB>ow</SUB> 5-8. Greater concentrations of PAHs and SOs were also found in those fractions. On the basis of GC-QTOFMS and GC-MSD analyses, 16 candidates for AhR agonists were identified in extracts of sediments. Of these, seven compounds, including 1-methylchrysene, benzo[<I>j</I>]fluoranthene, 3-methylchrysene, 5-methylbenz[<I>a</I>]anthracene, 11<I>H</I>-benzo[<I>b</I>]fluorene, benzo[<I>b</I>]naphtho[2,3-<I>d</I>]furan, and benzo[<I>b</I>]naphtho[2,1-<I>d</I>]thiophene, exhibited significant AhR activity. Relative potency values of newly identified AhR agonists were found to be greater than or comparable to that of benzo[<I>a</I>]pyrene (BaP). The potency balance analysis showed that newly identified AhR agonists explained 0.07-16% of bioassay-derived BaP-EQs. These chemicals were widely distributed in industrial sediments; thus, it is of immediate importance to conduct studies on sources and potential effects of those chemicals.</P> [FIG OMISSION]</BR>

Major AhR-active chemicals in sediments of Lake Sihwa, South Korea: Application of effect-directed analysis combined with full-scan screening analysis

Cha, Jihyun,Hong, Seongjin,Kim, Jaeseong,Lee, Junghyun,Yoon, Seo Joon,Lee, Sunggyu,Moon, Hyo-Bang,Shin, Kyung-Hoon,Hur, Jin,Giesy, John P.,Khim, Jong Seong Elsevier 2019 Environment international Vol.133 No.2

<P><B>Abstract</B></P> <P>This study utilized effect-directed analysis (EDA) combined with full-scan screening analysis (FSA) to identify aryl hydrocarbon receptor (AhR)-active compounds in sediments of inland creeks flowing into Lake Sihwa, South Korea. The specific objectives were to (i) investigate the major AhR-active fractions of organic extracts of sediments by using H4IIE-<I>luc in vitro</I> bioassay (4 h and 72 h exposures), (ii) quantify known AhR agonists, such as polycyclic aromatic hydrocarbons (PAHs) and styrene oligomers (SOs), (iii) identify unknown AhR agonists by use of gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOFMS), and (iv) determine contributions of AhR agonists to total potencies measured by use of the bioassay. FSA was conducted on fractions F2.6 and F2.7 (aromatics with log K<SUB>ow</SUB> 5–7) in extracts of sediment from Siheung Creek (industrial area). Those fractions exhibited significant AhR-mediated potency as well as relatively great concentrations of PAHs and SOs. FSA detected 461 and 449 compounds in F2.6 and F2.7, respectively. Of these, five tentative candidates of AhR agonist were selected based on NIST library matching, aromatic structures and numbers of rings, and available standards. Benz[<I>b</I>]anthracene, 11H-benzo[<I>a</I>]fluorene, and 4,5-methanochrysene exhibited significant AhR-mediated potency in the H4IIE-<I>luc</I> bioassay, and relative potencies of these compounds were determined. Potency balance analysis demonstrated that these three newly identified AhR agonists explained 1.1% to 67% of total induced AhR-mediated potencies of samples, which were particularly great for industrial sediments. Follow-up studies on sources and ecotoxicological effects of these compounds in coastal environments would be required.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EDA combined with FSA identified unknown toxicants in industrial sediments. </LI> <LI> Benz[<I>b</I>]anthracene, 11H-benzo[<I>a</I>]fluorene, and 4,5-methanochrysene are novel AhR agonists. </LI> <LI> Benz[<I>b</I>]anthracene showed 10-fold greater AhR potency compared to benzo[<I>a</I>]pyrene. </LI> <LI> Newly identified AhR agonists significantly contributed to AhR potencies from sediments. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Managing Mental Health during the COVID-19 Pandemic: Recommendations from the Korean Medicine Mental Health Center

Hyo-Weon Suh,Sunggyu Hong,Hyun Woo Lee,Seok-In Yoon,Misun Lee,Sun-Yong Chung,Jong Woo Kim 대한한의학회 2022 대한한의학회지 Vol.43 No.4

생물영향동정평가 기법을 이용한 포항 산업지역 퇴적물의 독성원인물질 검색 : 유세포분석기를 이용한 세포건강성 평가의 적용

안성아(Seong-Ah An),홍성진(Seongjin Hong),이정현(Junghyun Lee),차지현(Jihyun Cha),이성규(Sunggyu Lee),문효방(Hyo-Bang Moon),김종성(Jong Seong Khim) 한국해양환경·에너지학회 2020 한국해양환경·에너지학회 학술대회논문집 Vol.2020 No.7

<i>Artemisia asiatica</i> ethanol extract exhibits anti-photoaging activity

Jeong, Deok,Lee, Jongsung,Jeong, Seong-Gu,Hong, Yo Han,Yoo, Sulgi,Han, Sang Yun,Kim, Ji Hye,Kim, Sunggyu,Kim, Jin Sic,Chung, Young Soo,Kim, Jong-Hoon,Yi, Young-Su,Cho, Jae Youl Elsevier 2018 Journal of Ethnopharmacology Vol.220 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P> <I>Artemisia asiatica</I> Nakai is a traditional herbal plant that has long been used in anti-inflammatory, anti-infective and skin protective remedies.</P> <P><B>Aim of the study</B></P> <P>In this study, traditionally known skin-protective activity of <I>Artemisia asiatica</I> Nakai was examined with its ethanol extract (Aa-EE) under various photoaging conditions using skin-originated cells, and the underlying mechanism was also examined using various types of cells.</P> <P><B>Materials and methods</B></P> <P>Effects of Aa-EE on cell viability, photocytotoxicity, and expression of matrix metalloproteinases (MMPs), cyclooxygenase (COX)-2, and moisturizing factors were measured in B16F10, HEK293, NIH3T3, and HaCaT cells under untreated and ultraviolet B (UVB)-irradiation conditions. Anti-melanogenic effect of Aa-EE was also examined by measuring both melanin content in B16F10 cells and tyrosinase activity. Anti-photoaging mechanism of Aa-EE was explored by determining the activation levels of signaling molecules by immunoblotting analysis.</P> <P><B>Results</B></P> <P>Aa-EE protected HaCaT cells from UVB irradiation-induced death. Aa-EE increased the expression of a type 1 pro-collagen gene and decreased the expression of matrix metalloproteinases, and COX-2 in NIH3T3 cells induced by UVB. Aa-EE increased the expression of transglutamase-1, hyaluronic acid synthase (HAS)-2, and HAS-3 in HaCaT cells and decreased the production of melanin in α-melanocyte-stimulating hormone-stimulated B16F10 cells by suppressing tyrosinase activity and the expression of tyrosinase, microphthalmia-associated transcription factor, tyrosinase-related protein (TRP)-1 and TRP-2.</P> <P><B>Conclusion</B></P> <P>The results suggest that Aa-EE could be skin-protective remedy with anti-photoaging, anti-apoptotic, skin remodeling, moisturizing, and anti-melanogenesis properties.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Anti-inflammatory functions of the CDC25 phosphatase inhibitor BN82002 via targeting AKT2

Kim, Han Gyung,Yang, Woo Seok,Hong, Yo Han,Kweon, Dae-Hyuk,Lee, Jongsung,Kim, Sunggyu,Cho, Jae Youl Elsevier 2019 Biochemical pharmacology Vol.164 No.-

<P><B>Abstract</B></P> <P>This study presents BN82002 as an anti-inflammatory drug candidate. It was found that BN82002 inhibited the production of nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in RAW 264.7 cells and peritoneal macrophages that were activated by toll-like receptor (TLR) 4 ligand, lipopolysaccharide (LPS). BN82002 dose-dependently down-regulated mRNA levels of nitric oxide synthase, tumor necrosis factor-α, and cyclooxygenase-2. The nuclear translocation of nuclear factor (NF)-κB (p65 and p50) was also blocked by BN82002 in RAW265.7 cells stimulated by LPS. According to reporter gene assay performed with NF-κB construct, BN82002 clearly reduced increased level of luciferase activity mediated by transcription factor NF-κB in LPS-treated RAW264.7 cells and in MyD88- and AKT2-overexpressing HEK293 cells. However, BN82002 did not inhibit NF-κB activity in AKT1- or IKKβ-overexpressing HEK293 cells. NF-κB upstream signaling events specifically targeted AKT2 but had no effect on AKT1. The specific target of BN82002 was Tyr-178 in AKT2. BN82002 bound to Tyr-178 and interrupted the kinase activity of AKT2, according to a cellular thermal shift assay analysis of the interaction of BN82002 with AKT2 and an AKT2 mutant (Tyr-178 mutated to Ala; AKT2 Y178A). These results suggest that BN82002 could reduce inflammatory pathway by controlling NF-κB pathway and specifically targeting AKT2.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Anti-Inflammatory Effect of <i> Piper attenuatum</i> Methanol Extract in LPS-Stimulated Inflammatory Responses

Kim, You Jin,Deok, Jeong,Kim, Sunggyu,Yoon, Deok Hyo,Sung, Gi-Ho,Aravinthan, Adithan,Lee, Seungihm,Lee, Mi-nam,Hong, Suntaek,Kim, Jong-Hoon,Son, Young-Jin,Cho, Jae Youl Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-

<P><I>Piper attenuatum </I>is used as a traditional medicinal plant in India. One of the substances in<I> P. attenuatum</I> has been suggested to have anti-inflammatory effects. However, there is insufficient research about the anti-inflammatory mechanisms of action of<I> P. attenuatum</I>. The effects of<I> P. attenuatum</I> methanol extract (Pa-ME) on the production of inflammatory mediators nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>), the expression of proinflammatory genes, the translocation level of transcription factors, and intracellular signaling activities were investigated using macrophages. Pa-ME suppressed the production of NO and PGE<SUB>2</SUB> in lipopolysaccharide- (LPS-), pam3CSK4-, and poly(I:C)-stimulated RAW264.7 cells without displaying cytotoxicity. The mRNA expression levels of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2) were decreased by Pa-ME. P-ME reduced the translocation of p50/NF-<I>κ</I>B and AP-1 (c-Jun and c-Fos), as well as the activity of their upstream enzymes Src, Syk, and TAK1. Immunoprecipitation analysis showed failure of binding between their substrates, phospho- (p-) p85 and p-MKK3/6. p-p85 and p-MKK3/6, which were induced by overexpression of Src, Syk, and TAK1, were also reduced by Pa-ME. Therefore, these results suggest that Pa-ME exerts its anti-inflammatory effects by targeting Src and Syk in the NF-<I>κ</I>B signaling pathway and TAK1 in the AP-1 signaling pathway. </P>