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RISS 인기검색어
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- 저자 : Sila JIN (검색결과 8 건)
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Fu, Cuicui,Jin, Sila,Shi, Wenbing,Oh, Joohee,Cao, Haiyan,Jung, Young Mee American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.22
<P>A novel surface-enhanced Raman spectroscopy (SERS) sensor was proposed for an ultrasensitive detection immunoassay based on tyramine signal amplification (TSA) strategy. In this study, an immune sandwich was prepared with a capture antibody and a horseradish peroxidase (HRP)-conjugated antibody upon the addition of a target antigen. In the presence of H<SUB>2</SUB>O<SUB>2</SUB>, HRP can convert tyramine to a short-lived radical intermediate that forms covalent compounds with aromatic amino acids on the surfaces of proteins. By labeling the tyramine with SERS tags in the form of gold nanoparticles (AuNPs) functionalized with a Raman-active probe (4-mercaptobenzoic acid, 4-MBA), AuNPs@4-MBA was deposited and aggregated near the proteins, so the SERS signal of 4-MBA could be detected and amplified. On the basis of the TSA strategy, the developed SERS-based immunoassay can discriminate concentrations as low as 0.01 ng/mL of the target antigen and exhibited approximately 10 times stronger SERS signal intensity than traditional SERS-based immunoassays. These results demonstrated the application potential of this TSA-based SERS biosensor for the detection of important proteins in biomedical research.</P> [FIG OMISSION]</BR>
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Fu, Cuicui,Jin, Sila,Oh, Joohee,Xu, Shuping,Jung, Young Mee The Royal Society of Chemistry 2017 The Analyst Vol.142 No.16
<P>A facile surface-enhanced Raman scattering (SERS) sensor based on a silver-ion-mediated amplification effect was designed for the determination of glucose concentration. In this approach, 4-aminothiophenol (4-ATP) molecules assembled on the surface of a gold wafer (Au wafer@4-ATP) act not only as Raman tags but also as linkage agents. Silver nanoparticles marked with cysteamine (AgNP@cys) were used as the SERS enhancement components because they could be bound to the Au wafer@4-ATP in the presence of silver ions through the formation of N → Ag<SUP>+</SUP> ← N coordination compounds. Here, the Ag<SUP>+</SUP> ions were obtained by using glucose oxidase to catalyze the oxidation of glucose, producing hydrogen peroxide (H2O2) to etch the AgNPs. Therefore, we recorded the SERS intensity of 4-ATP to determine the concentration of glucose in a phosphate buffer as low as 0.1 mM and further achieved a lowest detection of 0.5 mM glucose in human serum. These results show that the proposed approach has strong potential for practical applications.</P>
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Formation mechanism of BAMLET by 2D Raman correlation analysis
Park, Yeonju,Park, Yujeong,Jin, Sila,Kim, Jung Won,Jung, Young Mee Elsevier 2018 Journal of molecular structure Vol.1171 No.-
<P><B>Abstract</B></P> <P>A partially unfolded bovine α-lactalbumin (ALA) and oleic acid (OA) complex called bovine α-lactalbumin made lethal to tumor cells (BAMLET) is a tumoricidal complex. For the first time, this study investigated the pH-induced formation mechanism of the ALA/OA complex using Raman spectroscopy. For a deeper understanding of the formation mechanism of the ALA/OA complex with decreasing pH, principal component analysis (PCA) and two-dimensional correlation spectroscopy (2D-COS) were applied to pH-dependent Raman spectra of the ALA/OA complex. Based on the results of the PCA and 2D-COS analyses, we successfully elucidated the formation mechanism of the ALA/OA complex at the molecular level. We also confirm that the ALA/OA complex formed below pH 3.0.</P> <P><B>Highlights</B></P> <P> <UL> <LI> For the first time, we investigated the pH-induced formation mechanism of the α-lactalbumin/oleic acid (ALA/OA) complex using Raman spectroscopy. </LI> <LI> We successfully elucidated the formation mechanism of the ALA/OA complex at the molecular level. </LI> <LI> Results of the PCA and 2D-COS analyses showed the ALA/OA complex formed below pH 3.0. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Li, Yali,Li, Qianwen,Sun, Chengbin,Jin, Sila,Park, Yeonju,Zhou, Tieli,Wang, Xu,Zhao, Bing,Ruan, Weidong,Jung, Young Mee Elsevier 2018 APPLIED SURFACE SCIENCE - Vol.427 No.2
<P><B>Abstract</B></P> <P>A new type of surface-enhanced Raman scattering (SERS) substrate was fabricated through the layer-by-layer self-assembly of silver nanoparticles (AgNPs, av. 45nm in diameter) and porous gold nanoclusters/nanoparticles (AuNPs, av. 143nm in diameter). The development of the porosity of the AuNPs was investigated, and successful SERS applications of the porous AuNPs were also examined. As compared with AgNP films, the enhancement factor of Ag-Au compound substrates is increased 6 times at the concentration of 10<SUP>−6</SUP> M. This additional enhancement contributes to the trace-amount-detection of target molecules enormously. The contribution is generated through the increase of the usable surface area arising from the nanoscale pores distributed three-dimensionally in the porous AuNPs, which enrich the adsorption sites and hot spots for the adsorption of probe molecules, making the developed nanofilms highly sensitive SERS substrates. The substrates were used for the detection of a physiological metabolite of urea molecules. The results reached to a very low concentration of 1mM and exhibited good quantitative character over the physiological concentration range (1∼20mM) under mimicking biophysical conditions. These results show that the prepared substrate has great potential in the ultrasensitive SERS-based detection and in SERS-based biosensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new type of SERS substrate, consisting of silver nanoparticles and porous gold nanoparticles was fabricated. </LI> <LI> An additional enhancement contribution generated from the porous characteristic of gold nanoparticles. </LI> <LI> The substrates were used for the detection of a physiological metabolite of urea molecules. </LI> <LI> The results exhibited good quantitative character over all the physiological concentration range (1∼20 mM). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Chen, Lei,Ma, Ning,Park, Yeonju,Jin, Sila,Hwang, Hoon,Jiang, Dayu,Jung, Young Mee Elsevier 2018 Spectrochimica acta. Part A, Molecular and biomole Vol.197 No.-
<P><B>Abstract</B></P> <P>In this paper, we introduced Raman spectroscopy techniques that were based on the traditional Fe<SUP>3+</SUP> determination method with phenanthroline as a probe. Interestingly, surface-enhanced Raman spectroscopy (SERS)-based approach exhibited excellent sensitivities to phenanthroline. Different detection mechanisms were observed for the RR and SERS techniques, in which the RR intensity increased with increasing Fe<SUP>3+</SUP> concentration due to the observation of the RR effect of the phenanthroline-Fe<SUP>2+</SUP> complex, whereas the SERS intensity increased with decreasing Fe<SUP>3+</SUP> concentration due to the observation of the SERS effect of the uncomplexed phenanthroline. More importantly, the determination sensitivity was substantially improved in the presence of a SERS-active substrate, giving a detection limit as low as 0.001μg/mL, which is 20 times lower than the limit of the UV–vis and RR methods. Furthermore, the proposed SERS method was free from other ions interference and can be used quality and sensitivity for the determination of the city tap water.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We designed the RR and SERS spectroscopic techniques based determination methods for Iron (III). </LI> <LI> Different detection mechanisms were observed for the RR and SERS techniques for the proposed system. </LI> <LI> The determination sensitivity was substantially improved in the presence of a SERS-active substrate. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>Different detection mechanisms were observed for the RR and SERS techniques, in which the RR intensity increased with increasing Fe<SUP>3+</SUP> concentration due to the observation of the RR effect of the phenanthroline-Fe<SUP>2+</SUP> complex, whereas the SERS intensity increased with decreasing Fe<SUP>3+</SUP> concentration due to the observation of the SERS effect of the uncomplexed phenanthroline.</P> <P>[DISPLAY OMISSION]</P>
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A reagent-assisted method in SERS detection of methyl salicylate
Li, Yali,Li, Qianwen,Wang, Yanan,Oh, Joohee,Jin, Sila,Park, Yeonju,Zhou, Tieli,Zhao, Bing,Ruan, Weidong,Jung, Young Mee Elsevier 2018 Spectrochimica acta. Part A, Molecular and biomole Vol.195 No.-
<P><B>Abstract</B></P> <P>With the explosive application of methyl salicylate (MS) molecules in food and cosmetics, the further detection of MS molecules becomes particularly important. Here we investigated the detection of MS molecules based on surface-enhanced Raman scattering (SERS) in a novel molecule/assistant/metal system constructed with MS, 4,4′-(hexafluoroisopropylidene) bis (benzoic acid) and Ag nanoparticles (AgNPs). The minimum detection concentration is 10<SUP>−4</SUP> M. To explore the function of assisted reagent, we also referred another system without assistant molecules. The result demonstrates that SERS signals were not acquired, which proves that the assistant molecules are critical for the capture of MS molecules. Two possible mechanisms of MS/assistant/AgNPs system were speculated through two patterns of hydrogen bonds. The linker molecules acted as the role of the bridge between metallic substrates and target molecules through the molecular recognition. This strategy is very beneficial to the expanding of MS detection techniques and other hydrogen bond based coupling detections with SERS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel molecule/assistant/metal system was constructed to achieve the SERS detection of methyl salicylate. </LI> <LI> The intermolecular hydrogen bond was employed as the driving force to accomplish the assembly. </LI> <LI> The obstacle of non-adsorption of target molecules on substrates was solved through the hydrogen-bond-based assembly method. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Kim, Sangsik,Yoo, Hee Young,Huang, Jun,Lee, Yongjin,Park, Sohee,Park, Yeonju,Jin, Sila,Jung, Young Mee,Zeng, Hongbo,Hwang, Dong Soo,Jho, YongSeok American Chemical Society 2017 ACS NANO Vol.11 No.7
<P>Adhesive systems in many marine organisms are postulated to form complex coacervates (liquid liquid phase separation) through a process involving oppositely charged polyelectrolytes. Despite this ubiquitous speculation, most well characterized mussel adhesive proteins are cationic and polyphenolic, and the pursuit of the negatively charged proteins required for bulk complex coacervation formation internally remains elusive. In this study, we provide a clue for unraveling this paradox by showing the bulky fluid/fluid separation of a single cationic recombinant mussel foot protein, rmfp-1, with no additional anionic proteins or artificial molecules, that is triggered by a strong cation-pi interaction in natural seawater conditions. With the similar condition of salt concentration at seawater level (>0.7 M), the electrostatic repulsion between positively charged residues of mfp-1 is screened significantly, whereas the strong cation-pi interaction remains unaffected, which leads to the macroscopic phase separation (i.e., bulky coacervate formation). The single polyelectrolyte coacervate shows interesting mechanical properties including low friction, which facilitates the secretion process of the mussel. Our findings reveal that the cation-pi interaction modulated by salt is a key mechanism in the mussel adhesion process, providing new insights into the basic understanding of wet adhesion, self-assembly processes, and biological phenomena that are mediated by strong short-range attractive forces in water.</P>
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