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- 저자 : Siew Ling Hii (검색결과 3 건)
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Hii Siew-Ling,Ling Yok-Siong,Koh Chen-Chung 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
Bee pollen, as one of the products of bees, is often claimed as nature’s most complete food. The present work aimed to evaluate and compare the physicochemical properties, proximate composition, and phytochemical content of stingless bee pollen Heterotrigona itama from eight different geographical origins in Sarawak, Malaysia. Storage studies of the stingless bee pollen samples at four different temperatures were also conducted. The pH and water activity of the pollen samples were in the range of 3.45 to 3.69 and 0.39 to 0.51, respectively. Pollen samples contained 51.96% carbohydrates, 16.31% protein, 4.85% lipid, and 2.24% minerals on average. In addition, the total phenolic content (TPC) and antioxidant activity of pollen extracts ranged from 27.99 to 63.17 mg GAE/g and 66.73 to 94.03% radical scavenging activity, respectively. The phytochemical screening test revealed that saponin, terpenoid, and flavonoids were present in all the pollen extracts under investigation. Processed bee pollen is relatively stable at a storage temperature of or below 40 ◦ C. In addition, no obvious degradation in terms of protein content, TPC or antioxidant scavenging activity was observed, even at an in cubation temperature of 50 ◦ C. The results of the present study indicated that processed stingless bee pollen of Sarawak origin is a natural product that could last a long time at an ambient temperature of 25 ◦ C in addition to its good source of nutritional and bioactive compounds.
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Chien Wei Ooi,Beng Ti Tey,Siew Ling Hii,Arbakariya Ariff,Ho Shing Wu,John Chi Wei Lan,Ruey Shin Juang,Siti Mazlina Mustapa Kamal,Tau Chuan Ling 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6
An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study
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Improvements in Engineering Properties of Soils through Microbial-Induced Calcite Precipitation
Ng Wei Soon,Lee Min Lee,Tan Chew Khun,Hii Siew Ling 대한토목학회 2013 KSCE JOURNAL OF CIVIL ENGINEERING Vol.17 No.4
Microbial-Induced Calcite Precipitation (MICP) has recently emerged as a sustainable technique for soil improvement. This paper aims to study the effectiveness of MICP in improving the shear strength and reducing the hydraulic conductivity of soils. A species of Bacillus group, B. megaterium was used to trigger the calcite precipitation. The experimental variables included soil types (tropical residual soil and sand), soil densities (85%, 90%, and 95% of their respective maximum densities), and treatment conditions (untreated, treated with cementation reagents only, treated with B. megaterium only, and treated with B. megaterium and cementation reagents). The results showed that MICP could effectively improve shear strength and reduce hydraulic conductivity for both residual soil and sand. The improvements, however, varied with soil densities, soil types, and treatment conditions. With MICP treatment, the improvement ratios in shear strength of the residual soil specimens were significantly higher (1.41-2.64) than those of the sand specimens (1.14-1.25). On the contrary, the sand specimens resulted in greater hydraulic conductivity reduction ratios (0.09-0.15)than those of the residual soil specimens (0.26-0.45). These observations can be explained by the particle-particle contacts per unit volume and pore spaces in the soil specimens. Both soil specimens when treated with cementation reagents only exhibited slight alterations in the shear strength (ranging from 1.06-1.33) and hydraulic conductivity (ranging from 0.69-0.95). The results implied that natural calcite forming microorganisms only exist for insignificant amount. The amount of calcite precipitated in the treated residual soil specimens ranged from 1.080% to 1.889%. The increments of calcite content in the treated sand specimens were comparatively higher, ranging from 2.661% to 6.102%. The results from Scanning Electron Microscope (SEM) analysis confirmed the experimental findings.
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