http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Fangze Li,Shuhui Men,Shiwei Zhang,Juan Huang,Xuehua Puyang,Zhenqing Wu,Zhanbin Huang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.9
Low-quality soil for land reuse is a crucial problem in vegetation quality and especially to waste disposal sites in mining areas. It is necessary to find suitable materials to improve the soil quality and especially to increase soil microbial diversity and activity. In this study, pot experiments were conducted to investigate the effect of a mixed material of humic acid, super absorbent polymer and biochar on low-quality soil indexes and the microbial community response. The indexes included soil physicochemical properties and the corresponding plant growth. The results showed that the mixed material could improve chemical properties and physical structure of soil by increasing the bulk density, porosity, macro aggregate, and promote the mineralization of nutrient elements in soil. The best performance was achieved by adding 3 g·kg-1 super absorbent polymer, 3 g·kg-1 humic acid, and 10 g·kg-1 biochar to soil with plant total nitrogen, dry weight and height increased by 85.18%, 266.41% and 74.06%, respectively. Physicochemical properties caused changes in soil microbial diversity. Acidobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Firmicutes, Nitrospirae, Planctomycetes, and Proteobacteria were significantly positively correlated with most of the physical, chemical and plant indicators. Actinobacteria and Armatimonadetes were significantly negatively correlated with most measurement factors. Therefore, this study can contribute to improving the understanding of low-quality soil and how it affects soil microbial functions and sustainability.
Significant Underestimation of Gaseous Methanesulfonic Acid (MSA) over Southern Ocean
Yan, Jinpei,Jung, Jinyoung,Zhang, Miming,Xu, Suqing,Lin, Qi,Zhao, Shuhui,Chen, Liqi American Chemical Society 2019 Environmental science & technology Vol.53 No.22
<P>Methanesulfonic acid (MSA), derived from the oxidation of dimethylsulfide (DMS), has a significant impact on biogenic sulfur cycle and climate. Gaseous MSA (MSA<SUB>g</SUB>) has been often ignored in previous studies due to its quick conversion to particulate MSA (MSA<SUB>p</SUB>) and low concentrations. MSA<SUB>g</SUB>, MSA<SUB>p</SUB>, and nss-SO<SUB>4</SUB><SUP>2-</SUP> were observed simultaneously for the first time with high-time-resolution (1 h) in the Southern Ocean (SO). The mean MSA<SUB>g</SUB> level reached up to 3.3 ± 1.6 pptv, ranging from ∼24.5 pptv in the SO, contributing to 31% ± 3% to the total MSA (MSA<SUB>T</SUB>). A reduction of the MSA to nss-SO<SUB>4</SUB><SUP>2-</SUP> ratios by about 30% was obtained when MSA<SUB>g</SUB> was not accounted for in the calculation, indicating that MSA<SUB>g</SUB> was very important in the assessment of the biogenic sulfur contributions in the atmosphere. Mass ratios of MSA to nss-SO<SUB>4</SUB><SUP>2-</SUP> increased first and then decreased with the temperature from −10 to 5 °C, with a maximum value at the temperature of −3 °C. Positive correlations between MSA<SUB>g</SUB> to MSA<SUB>T</SUB> ratios and temperature were presented, when the temperature was higher than 5 °C. This study highlights the importance of MSA<SUB>g</SUB> for understanding the atmospheric DMS oxidation mechanism and extends the knowledge of MSA formation in the marine atmosphere.</P> [FIG OMISSION]</BR>
Kai Wen,Baiqing Xiong,Yongan Zhang,Zhihui Li,Xiwu Li,Shuhui Huang,Lizhen Yan,Hongwei Yan,Hongwei Liu 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.3
In the present work, the infl uence of various retrogression treatments on hardness, electrical conductivity and mechanicalproperties of a high Zn-containing Al–Zn–Mg–Cu alloy is investigated and several retrogression regimes subjected to a samestrength level are proposed. The precipitates are qualitatively investigated by means of transmission electron microscopy(TEM) and high-resolution transmission electron microscopy techniques. Based on the matrix precipitate observations, thedistributions of precipitate size and nearest inter-precipitate distance are extracted from bright-fi eld TEM images projectedalong ⟨110⟩ Al orientation with the aid of an imaging analysis and an arithmetic method. The results show that GP zonesand η′ precipitates are the major precipitates and the precipitate size and its distribution range continuously enlarge with theretrogression regime expands to an extent of high temperature. The nearest inter-precipitate distance ranges obtained arequite the same and the average distance of nearest inter-precipitates show a slight increase. The infl uence of precipitates onmechanical properties is discussed through the interaction relationship between precipitates and dislocations.
Kou, Geng,Gao, Jie,Wang, Hao,Chen, Huaiwen,Li, Bohua,Zhang, Dapeng,Wang, Shuhui,Hou, Sheng,Qian, Weizhu,Dai, Jianxin,Zhong, Yanqiang,Guo, Yajun Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
The purpose of this study was to develop paclitaxel-loaded poly(lactide-co-glycolide) (PLGA) nanoparticles coated with cationic SM5-1 single-chain antibody (scFv) containing a polylysine (SMFv-polylys). SM5-1 scFv (SMFv) is derived from SM5-1 monoclonal antibody, which binds to a 230 kDa membrane protein specifically expressed on melanoma, hepatocellular carcinoma and breast cancer cells. SMFv-polylys was expressed in Escherichia coli and purified by cation-exchange chromatography. Purified SMFv-polylys was fixed to paclitaxel-loaded PLGA nanoparticles to form paclitaxel-loaded PLGA nanoparticles coated with SMFv-polylys (Ptx-NP-S). Ptx-NP-S was shown to retain the specific antigen-binding affinity of SMFv-polylys to SM5-1 binding protein-positive Ch-hep-3 cells. Finally, the cytotoxicity of Ptx-NP-S was evaluated by a non-radioactive cell proliferation assay. It was demonstrated that Ptx-NP-S had significantly enhanced in vitro cytotoxicity against Ch-hep-3 cells as compared with non-targeted paclitaxel-loaded PLGA nanoparticles. In conclusion, our results suggest that cationic SMFv-polylys has been successfully generated and may be used as targeted ligand for preparing cancer-targeted nanoparticles.
Yu Wang,Zhihui Li,Baiqing Xiong,Kai Wen,Shuhui Huang,Xiwu Li,Yongan Zhang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3
Microstructure evolution of a new high-alloyed Al–Zn–Mg–Cu–Zr–Sc aluminium alloy during two-stage homogenizationprocess was investigated by use of scanning electron microscope, transition electron microscope and high resolution transitionelectron microscope. The results indicate that the morphology and chemical composition of Al 3 (Sc, Zr) particles formedin the fi rst stage were greatly aff ected by heating temperature. With the increase of heating temperature, the morphologyof Al 3 (Sc, Zr) particles transform from cuboidal with evident faceting to spheroidal due to improved Zr diff usivity. MoreZr atoms enrich in the interface of precipitate/matrix forming a thin layer. Moreover, the mean diameter of precipitatesincreases a little bit with the increase of heating temperature, showing very restricted coarsening rate and high thermal stabilityof Al 3 (Sc, Zr) particles. After an appropriate second stage heat treatment (474 °C × 48 h), the intermetallic formed inthe solidifi cation process could dissolve suffi ciently and Al 3 (Sc, Zr) particles still keep very good coherency with Al matrixwithout abnormal growth.
TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins
Linlin Sun,Guohong Yu,Xiaori Han,Shichao Xin,Xiaojing Qiang,Linlin Jiang,Shuhui Zhang,Xian-guo Cheng 한국식물학회 2015 Journal of Plant Biology Vol.58 No.5
Aquaporins (AQPs), a large family of channel proteins in plants, play an important role in regulating the balance of osmotic potential in cells. We isolated an AQP gene, TsMIP6, from the halophyte Thellungiella salsuginea and functionally characterized it in transgenic rice (Oryza sativa). This gene belongs to a subfamily of tonoplast intrinsic proteins and is localized at the plasma membrane. Real-time PCR showed that expression of TsMIP6 in shoots or roots of T. salsuginea was markedly induced by salinity, whereas its ectopic expression in ‘Kitaake’ lines of rice significantly increased plant tolerance to salt stress. Physiological data suggested that TsMIP6 is involved in regulating ion homeostasis and water channel activity in salt-stressed transgenic rice. Heterologous expression analysis indicated that TsMIP6 specifically interacts with a member of the glycoside hydrolase family 64 protein #617 in yeast cells. This suggests that the relationship between TsMIP6 and #617 has a crucial role in mediating osmotic balance in plant cells. Moreover, TsMIP6 might help to modulate the transport of some neutral molecules and may function through a pathway regulating solute equilibrium to maintain osmotic potential.