추간판 조직에서 연령 및 퇴행성 변화와 신생혈관과 섬유모세포 성장인자발현에 대한 연구

전창훈,김한겸,강신영 대한척추외과학회 1999 대한척추외과학회지 Vol.6 No.3

연구계획 : 추간판 조직에서 신생혈관의 연구는 탈출된 추간판 조직에서 이루어졌다. 본 연구는 퇴행성 척추질환의 추간판 조직에서 신생혈관과 섬유모세포 성장인자의 발현에 대한 연령 및 퇴행성 변화와의 관계를 연구하고자 한다. 연구목적 : 추간판 조직에서 신생혈관과 섬유모세포 성장인자의 발현을 관찰하고 추간판 조직의 연령 및 퇴행성 변화와의 상관관계를 연구하고자 한다. 대상 및 방법 : 주된 증상이 요통인 퇴행성 척추 질환으로 전방 추체유합술을 시행받고 12개월 이상 추시 가능하 였던 환자의 추간판 조직 30례를 연구대상으로 하였다. 조직학적 관찰은 H-E염색과 혈관내피세포 성장인자와 섬유 모세포 성장인자를 이용한 면역 조직화학염색을 실시하였다. 대조군으로 부검시 채취한 신생아의 추간판 조직 12례를 대상으로 하였다. 결과 : 질환군에서 Hematoxylin - eosine염색 및 혈관내피세포 성장인자와 섬유모세포 성장인자를 이용한 면역 조직 화학염색상 신생혈관과 섬유모세포 성장인자의 발현은 전례에서 섬유륜의 바깥 1/2 층에서 관찰되었으나, 수핵에서는 혈관형성이나, 면역염색상 양성반응을 보이는 세포는 관찰되지 않았다. 대조군 12례의 추간판조직에서 Hematoxylin-eosin염색상 혈관형성이 관찰되었으나, 혈관내피세포 성장인자 항체와 섬유모세포 성장인자를 이용한 면역 염색상 양성 반응을 보이지 않는다. 대조군과 질환군 사이의 신생혈관에 대한 혈관내피세포 성장인자와 섬유 모세포 성장인자의 발현의 차이점은 통계학적으로 의미가 있었다( P<0.05). 질환군 사이의 신생혈관에 대한 혈관내 피세포 성장인자 항체 및 섬유모세포 성장인자의 발현의 차이는 없었다( P>0.05 ) . 결론 : 추간판내의 신생혈관과 섬유모세포 성장인자의 발현은 추간판 조직의 연령 및 퇴행성 변화와 관계가 있다 고 사료되었다. Numerous studies have shown neovascularization and basic fibroblast growth factor(bFGF) expression in herniated nucleus pulposus. It is not clear whether the neovascularization and bFGF expression are due to herniated nucleus pulposus with secondary reaction phenomenon or due to disc aging and degeneration. The purpose of this study is to investigate neovascularization and bFGF expression in both nucleus pulposus and anulus fibrosus tissues from aging and degenerated discs and compare it to neonate intervertebral disc. Thirty disc blocks obtained during anterior interbody fusion of the lumbar spine with degenerative spine disease were studied by immunochemical stains to observe neovascularization with vascular endothelial cell growth factor(VEGF) and bFGF expression. There were 8 disc blocks from the patients with degenerative disc disease, 15 with spondylolytic spondylolisthesis, and 7 with spinal stenosis with instability. For the control group, 12 neonate disc blocks obtained at autopsy. The neovascularization and bFGF expression were observed in the outer one half layer of the anulus fibrosus in the disease group but not in the nucleus pulposus. The neovascularization and bFGF expression were not observed in the control group. There was a statistical significant difference of neovascularization and bFGF expression between the control and disease group(p<0.05). There was no statistical significant difference of neovascularization and bFGF expression among the disease group(p>0.05). The results of this study suggest that neovascularization and bFGF expression are inherently related to aging and degeneration of the intervertebral disc.

Cruciferous Vegetables, Glutathione S-Transferases, and Implications of Their Interaction to Colorectal Cancer Risk: A Review

Kim, Jae Kyeom,Shin, Dong-Hoon,Park, Hui Gyu,Shin, Eui-Cheol The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4

Globally, colorectal cancer is the third most common type of cancer in men and the second most common in women. As the rate of this cancer increases with the degree of industrialization and urbanization, many researchers emphasize the importance of diets as a decisive factor in the etiology of this cancer. To be specific, the hypothesis that fruit and vegetable intake would act as preventive measurement against colorectal cancer has gained much interest for the general population as well as medical professionals. However, results of epidemiological studies were somewhat inconsistent and showed weak associations in this regard. One possible explanation regarding these controversial results could be due to limited understanding of the interaction between genetic variations and nutrients and their ability to impede cancer development. The objective of this review was to introduce the chemopreventive mechanisms of cruciferous vegetables as well as their active compounds. Furthermore, existing evidence regarding interactions between genetic variations in the key biotransformation enzyme (i.e., glutathione S-transferase) and the effect of the intake of cruciferous vegetables against the risk of colorectal cancer were critically discussed.

Protective Effects of Kaempferol (3,4′,5,7-tetrahydroxyflavone) against Amyloid Beta Peptide (Aβ)-Induced Neurotoxicity in ICR Mice

KIM, Jae Kyeom,CHOI, Soo Jung,CHO, Hong Yon,HWANG, Han-Joon,KIM, Young Jun,LIM, Seung Taik,KIM, Chang-Ju,KIM, Hye Kyung,PETERSON, Sabrina,SHIN, Dong-Hoon Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, biotechnology, and biochemistry Vol.74 No.2

<P>To determine the effects of kaempferol, rat pheochromocytoma cells (PC12) and Institute of Cancer Research (ICR) mice were utilized as neuronal models. Using <I>in vitro</I> assays, kaempferol was shown to have protective effects against oxidative stress-induced cytotoxicity in PC12 cells. Administration of kaempferol also significantly reversed amyloid beta peptide (Aβ)-induced impaired performance in a Y-maze test. Taken altogether, the results reported here suggest that further investigation is warranted of the influence of kaempferol on pathways related to Alzheimer’s disease.</P>

Proteomic analysis reveals overexpression of moesin and cytokeratin 17 proteins in colorectal carcinoma.

Kim, Chan Yong,Jung, Woon Yong,Lee, Hyun Joo,Kim, Han Kyeom,Kim, Aeree,Shin, Bong Kyung National Hellenic Research Foundation 2012 Oncology reports Vol.27 No.3

<P>The study of tumor biomarkers was gradually facilitated by the adoption of proteomic strategies due to less invasiveness and higher sensitivity. Colorectal cancer is one of the most commonly occurring cancers worldwide and its incidence has markedly increased in Korea. While the adoption of proteomic strategies facilitated the study of tumor biomarkers, to date, no common agreement has been derived from proteomic investigations regarding tumor markers of colorectal cancer. This study was designed to find molecules differentially expressed in colorectal cancer compared to non-tumor mucosa. Four colorectal adenocarcinoma and corresponding non-tumor tissue samples were analyzed to find previously unknown proteins via two-dimensional electrophoresis and MALDI-TOF/MS spectrometry. Western blot assays and tissue microarray (TMA) immunohistochemistry were performed to validate the identified proteins. Among the twelve up-regulated and one down-regulated proteins identified, moesin, cytokeratin (KRT) 17 and carbonic anhydrase??I were validated by western blot analysis and/or immunohistochemistry. On immunohistochemistry, both moesin and KRT17 demonstrated a tendency of increased expression as pT stage advanced. Both moesin and KRT17 were not expressed in normal colorectal epithelium. These two proteins may play a role in cancer invasion and/or metastasis in colorectal carcinoma, and could be candidate biomarkers for the diagnosis and prognosis of colorectal cancer.</P>

Responsive Surface Methodology Optimizes Extraction Conditions of Industrial by-products, <i>Camellia japonica</i> Seed Cake

Kim, Jae Kyeom,Lim, Ho-Jeong,Kim, Mi-So,Choi, Soo Jung,Kim, Mi-Jeong,Kim, Cho Rong,Shin, Dong-Hoon,Shin, Eui-Cheol Medknow PublicationsMedia Pvt Ltd 2016 Pharmacognosy magazine Vol.12 No.47

<P><B>Background:</B></P><P>The central nervous system is easily damaged by oxidative stress due to high oxygen consumption and poor defensive capacity. Hence, multiple studies have demonstrated that inhibiting oxidative stress-induced damage, through an antioxidant-rich diet, might be a reasonable approach to prevent neurodegenerative disease.</P><P><B>Objective:</B></P><P>In the present study, response surface methodology was utilized to optimize the extraction for neuro-protective constituents of <I>Camellia japonica</I> byproducts.</P><P><B>Materials and Methods:</B></P><P>Rat pheochromocytoma cells were used to evaluate protective potential of <I>Camellia japonica</I> byproducts.</P><P><B>Results:</B></P><P>Optimum conditions were 33.84 min, 75.24%, and 75.82°C for time, ethanol concentration and temperature. Further, we demonstrated that major organic acid contents were significantly impacted by the extraction conditions, which may explain varying magnitude of protective potential between fractions.</P><P><B>Conclusions:</B></P><P>Given the paucity of information in regards to defatted <I>C. japonica</I> seed cake and their health promoting potential, our results herein provide interesting preliminary data for utilization of this byproduct from oil processing in both academic and industrial applications.</P><P><B>SUMMARY</B></P><P><P>Neuro-protective potential of <I>C. japonica</I> seed cake on cell viability was affected by extraction conditions</P><P>Extraction conditions effectively influenced on active constituents of <I>C. japonica</I> seed cake</P><P>Biological activity of <I>C. japonica</I> seed cake was optimized by the responsive surface methodology.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviations used:</B> GC-MS: Gas chromatography-mass spectrometer, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, PC12 cells: Pheochromocytoma, RSM: Response surface methodology.</P>

Chemical Characterization and Oxidative Stability of Medium- and Long-Chain Fatty Acid Profiles in Tree-Borne Seed Oils

Kim, Da-Som,Kim, Hoe-Sung,Lee, Kyoung-Tae,Hong, Dong-Lee,Cho, Sung-Rae,Pan, Jeong Hoon,Park, Yong Bae,Lee, Yang-Bong,Kim, Jae Kyeom,Shin, Eui-Cheol Hindawi 2018 Journal of analytical methods in chemistry Vol.2018 No.-

<P>This study was undertaken to evaluate chemical characteristics and oxidative stability of tree-borne seed oils. A total of 15 different fatty acids were identified in six tree-borne seed oils, which included seven types of saturated fatty acids, four types of monounsaturated fatty acids, and four types of polyunsaturated fatty acids. Japanese camphor tree (JCT) had a high content of medium-chain fatty acids (97.94 ± 0.04%), in which fatty acid composition was distinct from those of the other five plant seed oils. Overall, contents of tocopherols, a type of fat-soluble vitamin, ranged between 3.82 ± 0.04 mg/100 g and 101.98 ± 1.34 mg/100 g, respectively. Phytosterol contents ranged from 117.77 ± 1.32 mg/100 g to 479.45 ± 4.27 mg/100 g, respectively. Of all tree-borne seed oils, <I>β</I>-sitosterol was the phytosterol at the highest concentration. Contents of unsaponifiables were between 0.13 ± 0.08 and 2.01 ± 0.02, and values of acid, peroxide, and <I>p</I>-anisidine were between 0.79 ± 0.01 and 38.94 ± 0.24 mg KOH/g, 3.53 ± 0.21 and 127.67 ± 1.79 meq/kg, and 2.07 ± 0.51 and 9.67 ± 0.25, respectively. Oxidative stability of tree-borne seed oils was assessed through measurement of oxidation-induction periods. These results should serve as a foundation to identify the potential of tree-borne seed oils in industrial application as well as in providing fundamental data.</P>

Inhibitory Effect of <i>Poncirus trifoliate</i> on Acetylcholinesterase and Attenuating Activity against Trimethyltin-Induced Learning and Memory Impairment

KIM, Jae Kyeom,BAE, Heyri,KIM, Mi-Jeong,CHOI, Soo Jung,CHO, Hong Yon,HWANG, Han-Joon,KIM, Young Jun,LIM, Seung Taik,KIM, Eun Ki,KIM, Hye Kyung,KIM, Bok Yong,SHIN, Dong-Hoon Japan Society for Bioscience, Biotechnology, and A 2009 Bioscience, biotechnology, and biochemistry Vol.73 No.5

<P>Various native Korean plants were screened to find an effective acetylcholinesterase (AChE) inhibitor for the treatment of Alzheimer’s disease (AD). Among these plants, the ethanol extract of <I>Poncirus trifoliate</I> was selected for isolating the AChE inhibitor because it exhibited the highest inhibitory activity (47.31%). To separate the active compound from <I>Poncirus trifoliate</I>, solvent partition, open column chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were utilized. The putative chemical structure of the AChE inhibitor was identified as <I>methoxsalen</I> by successive analysis with electron ionization mass spectrometry (EI-MS) and <SUP>13</SUP>C/<SUP>1</SUP>H-nuclear magnetic resonance (NMR). To confirm the attenuating effect of the <I>Poncirus trifoliate</I> extract against trimethyltin (TMT)-induced neurotoxicity, <I>in vivo</I> behavior tests were carried out. Our findings suggest that the <I>Poncirus trifoliate</I> extract significantly reversed TMT-induced learning and memory impairment. These results demonstrate that the <I>Poncirus trifoliate</I> extract could possess a wide range of beneficial activities for neurodegenerative disorders, notably AD.</P>

Comparison of nutritional quality and thermal stability between peanut oil and common frying oils

Kim, Jae Kyeom,Lim, Ho-Jeong,Shin, Dong-Hoon,Shin, Eui-Cheol The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4

The aim of this work was to compare the performances and nutritional characteristics of peanut oil to common frying oils during repetitive frying. The peanut oil had the highest color stability, the lowest initial, and final total polar compounds (TPC) levels among all other oil samples throughout the frying of potato slices. The TPC levels increased in all oils over the course of frying, being impacted more by the initial quality rather than the type of oils. In tocopherol analysis, as a consequence of frying, ${\gamma}$-tocopherol decreased most among the other six tocopherol isomers. Owing to the lack of data on the repetitive frying of peanut oil, this work provides basic information for peanut oil to use for frying purposes.

Quality Evaluation on Use of Camellia Oil as an Alternative Method in Dried Seaweed Preparation

Kim, Jae Kyeom,Park, Hui Gyu,Kim, Cho Rong,Lim, Ho-Jeong,Cho, Kye Man,Choi, Jine Shang,Shin, Dong-Hoon,Shin, Eui-Cheol The Korean Society of Food Science and Nutrition 2014 Preventive Nutrition and Food Science Vol.19 No.3

The fatty acid and volatile compound compositions of camellia oil were analyzed in this study. The impacts of the replacement of conventional vegetable oil with camellia oil on the sensory attributes of dried seaweed were also determined. C18:1 (83.59%), followed by C16:0 and C18:2, were the most abundant fatty acids in camellia oil. A total of 11 and 32 volatile compounds were identified in camellia oil and sesame oil, respectively. In the preference test, the camellia oil samples received a higher, although insignificant, liking rating in overall acceptability of appearance. Overall, there were no differences between the sensory attributes of camellia oil and sesame oil. This finding, combined with the unique fatty acid composition, thermal stability, and health benefits of camellia oil indicate that further study into the use of camellia oil in foods is warranted.

Effects of Methoxsalen from <i>Poncirus trifoliata</i> on Acetylcholinesterase and Trimethyltin-Induced Learning and Memory Impairment

KIM, Jae Kyeom,CHOI, Soo Jung,BAE, Hyeri,KIM, Cho Rong,CHO, Hong-Yon,KIM, Young Jun,LIM, Seung Taik,KIM, Chang-Ju,KIM, Hye Kyung,PETERSON, Sabrina,SHIN, Dong Hoon Japan Society for Bioscience, Biotechnology, and A 2011 Bioscience, Biotechnology, and Biochemistry Vol.75 No.10

<P>Previously, we identified methoxsalen (8-methoxy-2′,3′,6,7-furocoumarin) as the bioactive compound probably responsible for acetylcholinesterase (AchE) inhibition achieved by feeding crude extract of <I>Poncirus trifoliate.</I> To confirm the activity of methoxsalen, Institute of Cancer Research (ICR) mice were fed a control or a methoxsalen-supplemented diet for 4 weeks, and then learning and memory enhancing effects with respect to trimethyltin (TMT)-induced neurotoxicity were evaluated. The brain tissues of ICR mice were dissected after completion of the behavioral tests for biochemical analysis. Methoxsalen effectively reversed TMT-induced memory impairment on both Y-maze and passive avoidance tests. Brain AchE activity was inhibited by the oral consumption of all concentrations of methoxsalen. Moreover, the level of oxidative stress was significantly ameliorated in the groups on methodsalen containing diets. This is the first <I>in vivo</I> study conducted with methoxsalen in the field of AD research, and it indicates that further investigation of methoxsalen is warranted.</P>