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Wei Yang,Toshishige Shibamoto,Yuhichi Kuda,Tao Zhang,Mamoru Tanida,Yasutaka Kurata 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.1
Purpose: Patients treated with propranolol, a nonselective β-adrenoceptor antagonist, develop severe anaphylaxis, but the mechanism remains unknown. We determined effects of β1- and β2-adrenoceptor antagonists on the anaphylaxis-induced increase in vascular permeability in mice. Methods: In anesthetized ovalbumin-sensitized C57BL mice, mean arterial blood pressure (MBP) was measured, and Evans blue dye extravasation and hematocrit (Hct) were assessed at 20 minutes after antigen injection. The following pretreatment groups (n=7/group) were studied: (1) sensitized control (non-pretreatment), (2) propranolol, (3) the selective β2-adrenoceptor antagonist ICI 118,551, (4) the selective β1-adrenoceptor antagonist atenolol, (5) adrenalectomy, (6) the selective β2-adrenoceptor agonist terbutaline, and (7) non-sensitized groups. Results: The antigen injection decreased MBP, and increased Hct and vascular permeability in the kidney, lung, mesentery, and intestine, but not in the liver or spleen. Pretreatment with ICI 118,551, propranolol and adrenalectomy, but not atenolol, reduced the survival rate and augmented the increases in Hct and vascular permeability in the kidney, intestine, and lung as compared with the sensitized control group. Pretreatment with terbutaline abolished the antigen-induced alterations. Plasma epinephrine levels were increased significantly in the sensitize control mice. Conclusions: Blockade of β2-adrenoceptor can deteriorate systemic anaphylaxis by augmenting hyperpermeability-induced increase in plasma extravasation by inhibiting beneficial effects of epinephrine released from the adrenal glands in anesthetized mice.
Yohei Okada,Chiharu Shibamoto,Yukari Osumi,Chihiro Asano,Riho Takeuchi,Sachio Nabeshima,Shu Morioka,Koji Shomoto 대한파킨슨병및이상운동질환학회 2018 Journal Of Movement Disorders Vol.11 No.2
We present a 47-year-old right-handed woman with a 15-year history of writer's cramp provided with six sessions of cathodal transcranial direct current stimulation (tDCS) combined with her observation of the writing actions performed by a healthy subject plus electromyographic (EMG) biofeedback training to decrease EMG activities of her right forehand muscles during writing for 30 min, 1 or 2/week for 4 weeks. She showed the improvement of dystonic posture and writing speed while writing after the intervention. The writing movement and writing speed scores on a writer's cramp rating scale and writing time decreased. Our findings demonstrated that cathodal tDCS combined with action observation and EMG biofeedback training might improve dystonic writing movements in a writer's cramp patient.
Yeon Park, Jun,Young Kim, Hyun,Shibamoto, Takayuki,Su Jang, Tae,Cheon Lee, Sang,Suk Shim, Jae,Hahm, Dae-Hyun,Lee, Hae-Jeung,Lee, Sanghyun,Sung Kang, Ki Pergamon Press 2017 Bioorganic & medicinal chemistry letters Vol.27 No.17
<P><B>Abstract</B></P> <P>The biological activities of the ethanol extract from <I>Cirsium japonicum</I> var. <I>maackii</I> (ICF-1) and its major component, polyphenol cirsimaritin, were investigated as part of the search for possible alternative drugs for breast cancer. Three <I>in vitro</I> cell-based assays were used: the cell proliferation assay, tube-formation assay, and Western blot analysis. Both the ICF-1 extract and cirsimaritin inhibited the viability of HUVECs in a dose-dependent manner. The inhibition achieved was 36.89% at a level of 200μg/ml by the ICF-1 extract and 62.04% at a level of 100μM by cirsimaritin. The ICF-1 extract and cirsimaritin reduced tube formation by 12.69% at level of 25μg/ml and 32.18% at the levels of 6.25μM, respectively. Cirsimaritin inhibited angiogenesis by downregulation of VEGF, p-Akt and p-ERK in MDA-MB-231 cells, suggesting that cirsimaritin is potentially useful as an anti-metastatic agent. The present study demonstrated that <I>Cirsium japonicum</I> extract and its active component cirsimaritin is an excellent candidate as an alternative anti-breast cancer drug.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Isolation and Identification of Headspace Volatiles Formed in Heated Butter
Lee, Su-Rae,Macku, Carlos,Shibamoto, Takayuki 이화여자대학교 생명과학연구소 1991 생명과학연구논문집 Vol.2 No.-
Unsalted sweet butter was heated at 100,150,or 200℃ for 5h. The headspace volatiles formed were collected during heating using a simultaneous purging and solvent extraction apparatus. Among 140 peaks on a gas chromatogram of the headspace sample from butter heated at 200℃, 77 were positively identified by gas chromatography and gas chromatograpy/ mass spectrometry. Major compounds identified were 21 aldehydes, 12 fatty acids, 11 ketones, 10 nitrogen-and /or sulfur-containing compounds, 7 alkanes, 6ð-lactones, and 4 furans, which constituted over 85% of total volatiles recovered. The number of volatiles formed in a headspace of butter heated at 100 or 150℃ was much less than that formed at 200℃. However, all volatiles formed at 100 and 150℃ was much less than that formed at 200℃. However, all volatiles formed at 100 and 150℃ were found in a sample heated at 200℃.
Analysis before and after Cooking Processes of a Trace Chlorpyrifos Spiked in Polished Rice
Lee, Su-Rae,Mourer, Charles R.,Shibamoto, Takayuki 이화여자대학교 생명과학연구소 1991 생명과학연구논문집 Vol.2 No.-
Polished rice grains were fortified with 500 ppb of chlorpyrifos. The fortified residues were analyzed by gas chromatography with a nitrogen-phosphorus detector or a Hall detector. Chlorpyrifos and its breakdown product 3,5,6-trichloro-2-pyridinol were recovered from fortified rice grains in the levels of 456 and 3.4 ppb, respectively. Washing rice grains with water removed approximately 60% of the chlorpyrifos residues. Fortified chlorpyrifos was found in cooked rice at a level of 130 ppb, which was approximately 30% of the original quantity. Acid treatment was required to remove chlorpyrifos bound to proteins or carbohydrates in the cooked rice.
Ngan, Luong Thi My,Moon, Joon-Kwan,Shibamoto, Takayuki,Ahn, Young-Joon American Chemical Society 2012 Journal of agricultural and food chemistry Vol.60 No.36
<P>An assessment was made of the growth-inhibiting, bactericidal, and urease inhibitory activities of paeonol (PA), benzoic acid (BA), methyl gallate (MG), and 1,2,3,4,6-penta-<I>O</I>-galloyl-β-<SMALL>d</SMALL>-glucopyranose (PGG) identified in Paeonia lactiflora root, structurally related compounds, and four antibiotics toward three reference strains and four clinical isolates of Helicobacter pylori using broth dilution bioassay and Western blot. BA and PA showed strong bactericidal effect at pH 4, while MG and PGG were effective at pH 7. These constituents exhibited strong growth-inhibiting and bactericidal activity toward the five strains resistant to amoxicillin (minimal inhibitory concentration (MIC) 12.5 mg/L), clarithromycin (64 mg/L), metronidazole (64 mg/L), or tetracycline (15 mg/L), indicating that these constituents and the antibiotics do not share a common mode of action. Structural characteristics, such as types of functional groups and carbon skeleton, and hydrophobicity appear to play a role in determining the anti-H. pylori activity. H. pylori urease inhibitory activity of PGG was comparable to that of acetohydroxamic acid, while MG was less potent at inhibiting urease than thiourea. The UreB band disappeared at 250 mg/L PGG on Western blot, while the UreA bands were faintly visible at 1000 mg/L PGG. These constituents showed no significant cytotoxicity. Global efforts to reduce the level of antibiotics justify further studies on P. lactiflora root-derived materials containing MG, PA, and PGG as potential antibacterial products or lead molecules for the prevention or eradication from humans from diseases caused by H. pylori.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2012/jafcau.2012.60.issue-36/jf3035034/production/images/medium/jf-2012-035034_0009.gif'></P>
MRI Reveals Edema in Larynx (But Not in Brain) During Anaphylactic Hypotension in Anesthetized Rats
Ichiro Toyota,Mamoru Tanida,Toshishige Shibamoto,Mofei Wang,Yasutaka Kurata,Hisao Tonami 대한천식알레르기학회 2013 Allergy, Asthma & Immunology Research Vol.5 No.6
Purpose: Anaphylactic shock is sometimes accompanied by local interstitial edema due to increased vascular permeability. We performed magneticresonance imaging (MRI) to compare edema in the larynx and brain of anesthetized rats during anaphylactic hypotension versus vasodilator-inducedhypotension. Methods: Male Sprague Dawley rats were subjected to hypotension induced by the ovalbumin antigen (n=7) or a vasodilator sodiumnitroprusside (SNP; n=7). Apparent diffusion coefficient (ADC) and T2-relaxation time (T2RT) were quantified on MRI performed repeatedly for upto 68 min after the injection of either agent. The presence of laryngeal edema was also examined by histological examination. Separately, the occurrenceof brain edema was assessed by measuring brain water content using the wet/dry method in rats with anaphylaxis (n=5) or SNP (n=5) andthe non-hypotensive control rats (n=5). Mast cells in hypothalamus were morphologically examined. Results: Mean arterial blood pressure similarlydecreased to 35 mmHg after an injection of the antigen or SNP. Hyperintensity on T2-weighted images (as reflected by elevated T2RT) was foundin the larynx as early as 13 min after an injection of the antigen, but not SNP. A postmortem histological examination revealed epiglottic edema inthe rats with anaphylaxis, but not SNP. In contrast, no significant changes in T2RT or ADC were detectable in the brains of any rats studied. In separateexperiments, the quantified brain water content did not increase in either anaphylaxis or SNP rats, as compared with the non-hypotensive controlrats. The numbers of mast cells with metachromatic granules in the hypothalamus were not different between rats with anaphylaxis and SNP,suggesting the absence of anaphylactic reaction in hypothalamus. Conclusion: Edema was detected using the MRI technique in the larynx duringrat anaphylaxis, but not in the brain.