A Study of the Mechanism of Solid-Particle Enhanced Nucleate Boiling Heat Transfer at a Flat Heating Surface

Zhong Liang Liu,Ming Heng Shi,Guo Sheng Dai 대한기계학회 1998 Heat transter conference Vol.5 No.1

Knockdown of Ezrin by RNA Interference Reverses Malignant Behavior of Human Pancreatic Cancer Cells in Vitro

Zhong, Zhi-Qiang,Song, Mao-Min,He, Ying,Cheng, Shi,Yuan, Hui-Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Pancreatic cancer is one of the most aggressive tumors with a dismal prognosis. The membrane cytoskeletal crosslinker Ezrin participates in several functions including cell proliferation, adhesion, motility and survival. There is increasing evidence that Ezrin is overexpressed in vast majority of malignant tumors and regulates tumor progression. However, its roles in pancreatic cancer remain elusive. Methods: Three pairs of specific Ezrin siRNAs were designed and synthetized and screened to determine the most efficient one for construction of a hairpin RNA plasmid targeting Ezrin. After transfection into the Panc-1 pancreatic cancer cell line, real-time quantitative PCR and Western blotting were performed to examine the expression of mRNA and protein. The MTT method was applied to examine the proliferation and the drug sensibility to Gemcitabine. Flow cytometry was used to assess the cycle and apoptosis, while capacity for invasion was determined with transwell chambers. Furthermore, we detected phosphorylated-Erk1/2 protein and phosphorylated-Akt protein by Western blotting. Results: Real-time quantitative PCR and Western blotting revealed that Ezrin expression was notably down-regulated at both mRNA and protein levels by RNA interference (P< 0.01). Proliferation was inhibited and drug resistance to gemcitabine was improved (P< 0.05). Flow cytometry showed that the proportion of cells in the G1/G0 phase increased (P< 0.01), and in G2/M and S phases decreased (P< 0.05), with no apparent differences in apoptosis (P> 0.05). The capacity for invasion was markedly reduced (P< 0.01). In addition, down-regulating Ezrin expression had no effect on phosphorylated-Akt protein (P>0.05), but could decrease the level of phosphorylated-Erk1/2 protein (P< 0.05). Conclusions: RNA interference of Ezrin could inhibit its expression in the pancreatic cancer cells line Panc-1, leading to a potent suppression of malignant behavior in vitro. Assessment of potential as a target for pancreatic cancer treatment is clearly warranted.

Balloon dilation of epidermolysis bullosa-related esophageal strictures: A report of two cases

Wei-Zhong Zhou,Jing-Guo Xia,Zheng-Qiang Yang,Chun-Gao Zhou,Lin-Bo Zhao,Sheng Liu,Hai Bin Shi 소화기인터벤션의학회 2018 Gastrointestinal Intervention Vol.7 No.3

Epidermolysis bullosa (EB) is a very rare inherited disease featured with skin blistering resulting from minor trauma. Sometimes the esophageal mucosa could also be involved, which leads to esophageal strictures. Here we report two cases of EB-related esophageal strictures who were successfully treated with esophageal balloon dilations. The two cases with EB had severe dysphagia. Clinical examination showed signs of malnutrition, skin blisters and loss of toenails due to EB. They underwent careful fluoroscopic balloon dilation with 10- and 16-mm-sized balloon catheters, respectively. They could ingest soft and some solid foods after the procedure and maintained during the 20 months and 16 months follow-up periods.

Balloon dilation of epidermolysis bullosa-related esophageal strictures: A report of two cases

Wei-Zhong Zhou,Jing-Guo Xia,Zheng-Qiang Yang,Chun-Gao Zhou,Lin-Bo Zhao,Sheng Liu,Hai Bin Shi 소화기인터벤션의학회 2018 International journal of gastrointestinal interven Vol.7 No.3

Epidermolysis bullosa (EB) is a very rare inherited disease featured with skin blistering resulting from minor trauma. Sometimes the esophageal mucosa could also be involved, which leads to esophageal strictures. Here we report two cases of EB-related esophageal strictures who were successfully treated with esophageal balloon dilations. The two cases with EB had severe dysphagia. Clinical examination showed signs of malnutrition, skin blisters and loss of toenails due to EB. They underwent careful fluoroscopic balloon dilation with 10- and 16-mm-sized balloon catheters, respectively. They could ingest soft and some solid foods after the procedure and maintained during the 20 months and 16 months follow-up periods.

Self-assembly and Mechanism of L-Alanine-based Dihydrazide Derivative as Excellent Gelator of Organic Solvents

Wang, Chuan-Sheng,Wang, Xiao-Hong,Li, Zhi-Yuan,Wei, Wei,Shi, Zhong-Liang,Sui, Zhi-Tong Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

A new organogelator, L-Alanine dihydrazide derivative can self-assemble in various organic solvents and turned them into thermally reversible physical supramolecular organogels at extremely low concentrations (< 2 wt %). The gel-sol phase transition temperatures ($T_{GS}$) were determined as a function of gelator concentration and the corresponding enthalpies (${\Delta}H_g$) were extracted. Scanning electron microscopy (SEM) measurements revealed that the interspaces of fiber-like network structures were diminished with the increasing of the LMOG concentration. FT-IR spectroscopy studies revealed that hydrogen-bonding and hydrophobic interaction were the driving forces for the formation of the gels. Based on the data of XRD and molecular modeling, the possible packing modes for the formation of organogelator aggregates were proposed.

Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton

Ming-feng Jiang,Sheng-wei Li,Min Chen,Ying-fan Cai,Yong-fang Xie,Biao Li,Quan Sun,Huai-zhong Jiang,Zheng Pan,Yun-ling Gao,You-Lu Yuan,Yu-zheng Shi 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5

A cDNA encoding a novel cysteine proteinase inhibitor (CPI) was isolated from a gland mutant Xiangmian-18 of upland cotton during the pigments gland forming stage. The cDNA comprises 378 bp and encodes 125 amino acid residues with molecular mass of 13.8 kDa. It contains the conserved motif of cysteine protease inhibitors and belongs to the cystatin superfamily (Gln- Val-Val-Ala-Gly). The deduced amino acid sequences of the domains are highly similar to the normal upland cotton (96.8%). SDS-PAGE and western hybridization analysis showed that the expressed recombinant protein was recombinant CPI. The inhibitory activity of recombinant CPI was 46 u/μg which was measured by inhibiting the protease activity of papain. RT-PCR results indicated that the expression level of developing gland stage was higher than that of undeveloped gland stage.

Double-sided collaborative machining for propeller blade based on XYZ-3RPS hybrid kinematic machine

Xiangyu Guo,Rui Wang,Shi-Sheng Zhong 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.10

At present, propeller blades are machined single-sided with low efficiency. Thus, a propeller needs to be turned over after completion of the machining of the first side of the blade, which requires second clamping and causes a decline in accuracy. Therefore, a doublesided collaborative machining method for propeller blades is proposed herein. Two XYZ-3RPS hybrid kinematics machines were symmetrically distributed to machine both sides of a propeller blade simultaneously; therefore, the blade could be machined in clamping once, improving the machining efficiency and eliminating the accuracy decline caused by repetitive clamping. Moreover, a supporting device with rigid-flexible switching capability was developed to reduce the cantilever length of the blade, thereby eliminating the substantial deformation and vibration of the blade during the double-sided collaborative machining process to ensure machining accuracy. Additionally, the inverse kinematics formula for XYZ-3RPS hybrid kinematics machines was deduced and the elongation of each drive shaft through the cutter location point and cutter orientation was solved in this study. Thereafter, the decomposition method of the blade shift data was applied to obtain the deformation and vibration amplitude of the blade so that the performance of the double-sided cooperative machining could be quantitatively analyzed. Finally, experiments were conducted on the self-developed prototype, and the results verified the effectiveness of the double-sided collaborative machining method in reducing the deformation and vibration of a blade and improving machining efficiency.

Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model

Ya-Dong Zhang,Zhong-Sheng Zhu,Dong Zhang,Zhen Zhang,Bin Ma,Shi-Chang Zhao,Feng Xue 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

This study aimed to investigate the effect of Patched-1 (PTC1) and PTC2 silencing in a rat model, on Hedgehog (Hh) pathwaymediated recovery from spinal cord injury (SCI). An analytical emphasis on the relationship between the sonic hedgehog (Shh) pathway and nerve regeneration was explored. A total of 126 rats were divided into normal, sham, SCI, negative control (NC), PTC1-RNAi, PTC2-RNAi and PTC1/PTC2-RNAi groups. The Basso, Beattie and Bresnahan (BBB) scale was employed to assess hind limb motor function. Quantitative real-time polymerase chain reaction and western blotting were performed to examine the mRNA and protein levels of PTC1, PTC2, Shh, glioma-associated oncogene homolog 1 (Gli-1), Smo and Nestin. Tissue morphology was analyzed using immunohistochemistry, and immunofluorescent staining was conducted to detect neurofilament protein 200 (NF-200) and glial fibrillary acidic protein (GFAP). The PTC1/PTC2-RNAi group displayed higher BBB scores than the SCI and NC groups. Shh, Gli-1, Smo and Nestin expression levels were elevated in the PTC1/PTC2-RNAi group. PTC1 and PTC2 mRNA and protein expression was lower in the PTC1/PTC2-RNAi group than in the normal, sham and SCI groups. Among the seven groups, the PTC1/PTC2-RNAi group had the largest positive area of NF-200 staining, whereas the SCI group exhibited a larger GFAP-positive area than both the normal and the sham groups. The Shh pathway may provide new insights into therapeutic indications and regenerative recovery tools for the treatment of SCI. Activation of the Hh signaling pathway by silencing PTC1 and PTC2 may reduce inflammation and may ultimately promote SCI recovery.

Development of a Novel Spawn (Block Spawn) of an Edible Mushroom, Pleurotus ostreatus, in Liquid Culture and its Cultivation Evaluation

( Wei-rui Zhang ),( Sheng-rong Liu ),( Yun-bo Kuang ),( Shi-zhong Zheng ) 한국균학회 2019 Mycobiology Vol.47 No.1

Mushroom cultivation has gained increased attention in recent years. Currently, only four types of spawn, including sawdust spawn, grain spawn, liquid spawn, and stick spawn, are commonly available for mushroom cultivation. This limited spawn diversity has led to difficulty in selecting suitable inoculum materials in some cultivation. In this study, three small blocks of lignocellulosic agro-wastes and one block of a synthetic matrix were prepared as support for growing Pleurotus ostreatus in liquid medium. Mycelium-adsorbed blocks were then evaluated for their potential as block spawn for fructification. Our results indicated that the edible fungus was adsorbed and abundantly grew internally and externally on loofah sponge and synthetic polyurethane foam (PUF) supports and also has the ability to attach and grow on the surface of sugarcane bagasse and corncob supports. The mycelia of P. ostreatus adhered on corncob exhibited the highest metabolic activity, while those on the PUF showed the least activity. Mycelial extension rates of block spawns made of agro-waste materials were comparable to that of sawdust spawn, but the block spawn of PUF showed a significantly lower rate. No significant differences in cropping time and yield were observed among cultivations between experimental block spawns and sawdust spawns. Moreover, the corncob block spawn maintained its fruiting potential during an examined period of 6-month storage. The developed block spawn could be practically applied in mushroom cultivation.

Aero-engine Gas Path Performance Degradation Assessment Based on a Multi-objective Optimized Discrete Feddback Network

Zhi-Quan Cui,Shi-Sheng Zhong,Zhi-Qi Yan 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.6

In order to solve the problem of neural network algorithm for aero-engine’s gas path performance evaluation under high-dimensional evaluation index with non-equal weights, the trend analysis method and fault fingerprints are used to mine engine’s gas path performance characteristic parameters. A comprehensive weighting method based on game theory is proposed to optimize the weight value of each gas path performance characteristic parameter. A discrete feedback neural network with single-layer and binary output is established. The original gas path performance evaluation index is equivalently expanded according to the weight ratio, and the gas path state evaluation indexes with different weights are mapped into higher-dimensional equivalent evaluation indexes with equal weights. The network attractor is designed according to the engine gas path performance evaluation levels, and the design of discrete feedback neural network weights is transformed into multi-objective programming problem, and a particle swarm optimization algorithm with adaptive inertia weight is used to improve the efficiency and global search ability of particle swarm optimization. The experimental results shows that the proposed model and algorithm can provide a scientific and reasonable machine learning method for the evaluation of high-dimensional evaluation index with non-equal weights.