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        Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., Isolated from Soil

        ( Shehzad Abid Khan ),( Hyung Min Kim ),( Ju Hye Baek ),( Hye Su Jung ),( Che Ok Jeon ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9

        Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242<sup>T</sup> and B156<sup>T</sup>, were isolated from soil in South Korea. Cells of strain H242<sup>T</sup> were oxidase-positive and non-motile short rods, while those of strain B156<sup>T</sup> were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C<sub>16:0</sub>, cyclo-C<sub>17:0</sub>, and summed feature 3 (C<sub>16:1</sub> ω7c and/or C<sub>16:1</sub> ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242<sup>T</sup> and B156<sup>T</sup> were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242<sup>T</sup> and B156<sup>T</sup> formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242<sup>T</sup> and B156<sup>T</sup> was 24.6%. Strains H242<sup>T</sup> and B156<sup>T</sup> were most closely related to Ramlibacter ginsenosidimutans BXN5-27<sup>T</sup> and Ramlibacter monticola G-3-2<sup>T</sup> with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242<sup>T</sup> and R. ginsenosidimutans and between strain B156<sup>T</sup> and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242<sup>T</sup> and B156<sup>T</sup> represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242<sup>T</sup> (=KACC 21667 <sup>T</sup> =JCM 33922<sup>T</sup>) and B156T (=KACC 21665 <sup>T</sup> =JCM 33920<sup>T</sup>), respectively.

      • KCI등재

        Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil

        Kim Kyeong Ryeol,Kim Kyung Hyun,Khan Shehzad Abid,Kim Hyung Min,Han Dong Min,Jeon Che Ok 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

        Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria, designated strains R7T and R19T, were isolated from sandy and forest soil, South Korea, respectively. Both strains were non-motile rods showing catalase- and oxidase-positive activities. Both strains were shown to grow at 10–37°C and pH 6.0–9.0, and in the presence of 0–1.5% (w/v) NaCl. Strain R7T contained iso-C14:0, iso-C15:0, iso-C16:0, and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1 ω9c), whereas strain R19T contained iso-C11:0 3-OH, C16:1 ω7c alcohol, iso-C11:0, iso-C15:0, iso-C16:0, and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1 ω9c) as major cellular fatty acids (> 5%). Both strains contained ubiquinone- 8 as the sole isoprenoid quinone and phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid as the major polar lipids. The DNA G + C contents of strains R7T and R19T calculated from their genomes were 66.9 mol% and 68.9 mol%, respectively. Strains R7T and R19T were most closely related to Lysobacter panacisoli C8-1T and Lysobacter niabensis GH34-4T with 98.7% and 97.8% 16S rRNA sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains R7T and R19T formed distinct phylogenetic lineages within the genus Lysobacter. Based on phenotypic, chemotaxonomic, and molecular features, strains R7T and R19T represent novel species of the genus Lysobacter, for which the names Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. are proposed. The type strains of L. arenosi and L. solisilvae are R7T (= KACC 21663T = JCM 34257T) and R19T (= KACC 21767T = JCM 34258T), respectively.

      • KCI등재

        Preliminary Study on Effect of Lactiplantibacillus plantarum on Osteoporosis in the Ovariectomized Rat

        진은선,김지연,민중기,전상룡,최경효,Shehzad Abid Khan,문기성,정제훈 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.4

        Osteoporosis is a growing global health concern primarily associated with decreased estrogen in postmenopausal women. Recently, some strains of probiotics were examined for potential anti-osteoporotic effects. This study intended to evaluate the impacts of Lactiplantibacillus plantarum MGE 3038 strain (MGE 3038) in ovariectomized rats. For this purpose, twelve weeks old female Wistar rats (n=21; 250–300 g) were divided into 3 groups; ovariectomy (OVX) group, OVX/MGE 3038 group and Sham group (control). In these groups; two went through respective OVX and one had daily MGE 3038 administration through oral gavage. Prior to 16 weeks after OVX, we collected blood samples and extracted the tibiae. We scanned the extracted tibiae by invivo micro-computed tomography (micro-CT) and evaluated pathology by hematoxylin and eosin (H&E) and Masson’s trichrome staining. The serum levels of C-telopeptide of type I collagen (CTX), osteocalcin (OC), and the receptor activator of nuclear factor-ĸB ligand (RANKL) were examined. The OVX/MGE 3038 group showed increases in bone mineral density, trabecular bone volume, trabecular number, and trabecular thickness (Tb.Th), and a decrease in trabecular spacing than the OVX group. However, OVX/MGE 3038 group and control group were measurably comparable in Tb.Th. Micro-CT, H&E, and Masson’s trichrome findings exhibited increased preservation and maintenance of trabecular bone structure in the OVX/MGE 3038 group in comparison to the OVX group. In serum, the levels of CTX, OC and RANKL were significantly different between the OVX and OVX/MGE 3038 groups. Taken together, L. plantarum MGE 3038 could be helpful for the treatment of osteoporosis.

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