http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Wichanee Bankeeree ),( Pongtharin Lotrakul ),( Sehanat Prasongsuk ),( Seung Wook Kim ),( Hunsa Punnapayak ) 한국화학공학회 2016 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.54 No.6
Our aim was to optimize the production of cellulase-free thermoactive xylanase by Aureobasidium pullu-lans CBS 135684 with statistical methodology based on experimental designs. Among eleven variables, the nutrient sources that had significant effect on xylanase production were corncob, (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, xylose, KH<sub>2</sub>PO<sub>4</sub> and tween 80, identified by the initial screening method of Plackett-Burman. The optimum concentrations of these five components were subsequently investigated using response surface methodology. The optimal concentrations (g·l<sup>-1</sup>) for maximum production of xylanase were corncob, 39.0; (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 3.0; xylose, 1.8; KH<sub>2</sub>PO<sub>4</sub> 1.4; and tween 80, 1.4, respectively. An improved xylanase yield of 8.74 ± 0.84 U·ml<sup>-1</sup> was obtained with optimized medium which is 2.1-fold higher pro-duction than previously obtained results (4.10 ± 0.10 U·ml<sup>-1</sup>) after 48 h of cultivation. In addition, the xylanase produc-tion under optimal condition reached 10.09 ± 0.27 U·ml<sup>-1</sup> after 72 h of cultivation.
유하영,Xiaoguang Yang,김동섭,이수권,Pongtharin Lotrakul,Sehanat Prasongsuk,Hunsa Punnapayak,김승욱 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.6
In this study, dilute sulfuric acid pretreatment was performed to improve the sugars recovery from Korean Miscanthus straw. The effect of pretreatment conditions on solubilized xylose was fundamentally investigated for the efficient removal of xylan. The optimal conditions were determined using a statistical method, and were shown to be a temperature of 121.6°C, an acid concentration of 1.1%, and a reaction time of 12.8 min. The combined severity factor was shown to be 1.1 under the optimum conditions. Following the pretreatment, the solubilized xylose in liquid fraction was found to be 71.2%, and about 72.6% of the solid was recovered. After enzymatic hydrolysis, about 86.4% glucose conversion was achieved when the pretreated biomass was used as a substrate, with the conversion being improved 4-fold compared with the control (untreated). The hydrolysates, approximately 10 g/L glucose, were applied to the fermentation of Saccharomyces cerevisiae K35, and the ethanol yield was about 96%. The overall process was evaluated based on the material balance, and the results show that approximately 172 g bioethanol can be produced when 1,000 g Miscanthus straw is loaded into the process.