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Lee, Ilbok,Jeong, Gyoung Hwa,An, Soyeon,Kim, Sang- Wook,Yoon, Songhun Elsevier BV * North-Holland 2018 Applied Surface Science Vol.429 No.-
<P><B>Abstract</B></P> <P>Herein, MnNi-layered double hydroxides (LDH) were imbibed within the interlayers of graphene nanosheets. The anionic surfactant, sodium dodecyl sulfate played a role of graphite exfoliator adding interaction with metal cations. Using this process, layered MnNi-LDH-graphene nanocomposite was prepared without formation of graphene oxide. When applied into pseudocapacitor electrode, LDH-graphene with optimal ratio between Mn and Ni exhibited very stable cycle with 90% at 1400 cycles and high energy 47.29Whkg<SUP>−1</SUP> at the power density of 7473Wkg<SUP>−1</SUP>, which was attributed to highly stable layered LDH structure within conductive graphene layers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 3D MnNi-layered double hydroxides (LDH)/graphene were synthesized from graphite. </LI> </UL> </P> <P> <ce:list id='lis0010'> <ce:list-item id='lsti0010'> <ce:label>•</ce:label> <ce:para id='par0010' view='all'>Nanocomposite-on-graphene electrode provides fast and efficient diffusion of electrolyte ions to active material surface.</ce:para> </ce:list-item> <ce:list-item id='lsti0015'> <ce:label>•</ce:label> <ce:para id='par0015' view='all'>Mn1Ni3-LDH-G composite electrode shows optimum electrochemical performance.</ce:para> </ce:list-item> </ce:list> </P>
Lee, John Hwa,Chaudhari, Atul A,Oh, In Gyoung,Eo, Seong Kug,Park, Sang-Youel,Jawale, Chetan V Canadian Veterinary Medical Association = Associat 2015 Canadian journal of veterinary research Vol.79 No.3
<P>In this study, the immune responses to and protective efficacy of a live attenuated Salmonella-delivered vaccine candidate secreting the papA, papG, iutA, and clpG antigens of Escherichia coli were evaluated against infection with avian pathogenic E. coli (APEC) in layer chickens. Primary vaccination was done at age 7 d and booster vaccination at age 5 wk. The levels of intestinal secretory immunoglobulin A specific to the 4 antigens were significantly higher in the vaccinated group than in the control group. A potent lymphocyte-proliferation response and increased levels of interferon-gamma, interleukin-2, and interleukin-6 in the plasma and in culture supernatants of antigen-stimulated lymphocytes from the vaccinated group suggested significant induction of the cell-mediated immune response in this group compared with the control group. Upon challenge with a virulent APEC strain at 8 wk of age, the vaccinated group had no deaths, whereas the control group had a 15% mortality rate. In addition, the morbidity rate was significantly higher in the control group (55%) than in the vaccinated group (15%). Thus, giving primary and booster vaccination with the Salmonella-delivered APEC vaccine candidate significantly elevated both mucosal and cellular immune responses, which protected the chickens against colibacillosis.</P>
Sung-Min Seo,조일훈,Joo-Ho Kim,Jin-Woo Jeon,Eun-Gyoung Oh,Hong-Sik Yu,Soon-Bum Shin,Hee-Jung Lee,백세환 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.12
An enzyme-linked immunosorbent assay (ELISA)-on-a-chip (EOC) biosensor combined with cell concentration technology based on immuno-magnetic separation (IMS) was investigated for use as a potential tool for early screening of Listeria monocytogenes (L. monocytogenes) in food products. The target analyte is a well-known pathogenic foodborne microorganism and outbreaks of the food poisoning typically occur due to contamination of normal food products. Thus, the aim of this study was to develop a rapid and reliable sensor that could be utilized on a daily basis to test food products for the presence of this pathogenic microorganism. The sensor was optimized to provide a high detection capability (e.g., 5.9 × 103 cells/mL) and, to eventually minimize cultivation time. The cell density was condensed using IMS prior to analysis. Since the concentration rate of IMS was greater than 100-fold, this combination resulted in a detection limit of 54 cells/mL. The EOC-IMS coupled analytical system was then applied to a real sample test of fish intestines. The system was able to detect L. monocytogenes at a concentration of 2.4 CFU/g after pre-enrichment for 6 h from the onset of cell cultivation. This may allow us to monitor the target analyte at a concentration less than 1 CFU/g within a 9 h-cultivation provided a doubling time of 40 min is typically maintained. Based on this estimation, the EOC-IMS system can screen and detect the presence of this microorganism in food products almost within working hours.
Kerion celsi caused by Trichophyton rubrum in an elderly
( Sang Youl Yu ),( Min Woo Park ),( Moo Kyu Suh ),( Gyoung Yim Ha ),( Jong Im Lee ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1
Kerion celsi is a severe inflammatory type of tinea capitis that presents as a boggy mass studded with broken hairs, oozing purulent material from follicular orifices. This infection is caused most commonly by zoophilic or geophilic pathogens. Trichophyton(T.) rubrum is an anthropophilic dermatophyte that is found all over the world. It has become one of the most important causative agents in tinea unguium and tinea pedis. But, Kerion celsi caused by T. rubrum is rare. Kerion celsi is uncommon in adult. We report a case of kerion celsi caused by T. rubrum in a 72-year-old woman. She presented with localized tender erythematous plaques with pustules with oozing purulent material on the frontal scalp. A fungal culture from tissue of the lesions was grown on Sabouraud``s dextrose agar and showed typical whitish cottony colonies of T. rubrum. The nucleotide sequence of internal transcribed spacer for clinical isolate was identical to that of T. rubrum strain UZ1588_14(GenBank accession number KP326579.1). She was treated with 200mg of oral itraconazole daily for 3 months. The skin lesions improved 1 month after treatment, and recurrence has not been observed.
Lee, Young Sun,Kim, Gyoung Hee,Koh, Young Jin,Zhuang, Qiguo,Jung, Jae Sung The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.2
Pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit, can be divided into three biovars (biovars 1, 2, and 3). Strains belonging to biovar 1 produce phaseolotoxin and were isolated in Japan and Italy before 2008. Strains of biovar 2 produce coronatine instead of phaseolotoxin and have been isolated only in Korea. Strains belonging to biovar 3 produce neither phaseolotoxin nor coronatine and are responsible for the global outbreak of bacterial canker of kiwifruit in recent years. The biovar 3-specific primer set was developed in a previous work. In this study, two sets of PCR primers specific to strains of biovars 1 and 2, respectively, were developed based on random amplified polymorphic DNA analyses. Primers PsaJ-F and PsaJ-R produced a 481-bp region with genomic DNA of biovar 1 strains, whereas primers PsaK-F and PsaK-R amplified a 413-bp region present only in the genome of biovar 2 strains.
Causal Agents of Blossom Blight of Kiwifruit in Korea
Lee, Young-Sun,Han, Hyo-Shim,Kim, Gyoung-Hee,Koh, Young-Jin,Hur, Jae-Seoun,Jung, Jae-Sung The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.3
The causal agents of bacterial blossom blight in kiwifruit were isolated from flowers displaying symptoms in Korea. The pathogens were characterized by biochemical and physiological tests, and identified on the basis of 16S rDNA and 16S-23S internal transcribed spacer (ITS) sequences. Pathogenicity tests demonstrated that the blossom blight of kiwifruit in Korea is caused by two pathogens, Pseudomonas syringae pv. syringae and P. fluorescens. Carbon source utilization and DNA-DNA hybridization experiments confirmed P. fluorescens as one of the causal agents of blossom blight of kiwifruit. P. syringae pv. syringae and P. fluorescens can be distinguished from each other by the symptoms they produce in flowers. P. syringae pv. syringae primarily affected the stamen, while P. fluorescens caused rotting of all internal tissues of buds or flowers.
Lee, Young Mok,Jung, Jin Gyoung,Kim, Jin Nam,Park, Tae Sub,Kim, Tae Min,Shin, Sang Su,Kang, Dae Kyung,Lim, Jeong Mook,Han, Jae Yong Society for the Study of Reproduction [etc.] 2006 BIOLOGY OF REPRODUCTION Vol.75 No.3
<P>In this study, we proposed a testis-mediated germline chimera production system based on the transplantation of testicular cells directly into heterologous testes. The testicular cells of juvenile (4-wk-old) or adult (24-wk-old) Korean Ogol chickens with a recessive pigmentation inhibitory gene, with or without prior culture, were injected (2 x 10(7) cells/head) into the seminiferous tubules of juvenile or adult recipients with White Leghorn with a dominant pigmentation inhibitory gene in a 2 x 2 factorial arrangement. The localization of transplanted cells into the inner space of the seminiferous tubules was confirmed within 24 h after injection. Subsequent testcross analyses showed that 7.8% (5/64) of the recipients had chimeric status in their testes. The periods of time from transfer to hatching of the first progeny with black feathers were 38 and 45 days for adult cells transplanted into an adult recipient, 188 days for adult cells into a juvenile recipient, and 137 days for juvenile cells into a juvenile recipient. Culture of the testicular cells derived both colony-forming and monolayer-forming cells. The colony-forming cells were stained positively for periodic acid Schiff solution, and further reacted with anti-SSEA-1, anti-SSEA-3, and anti-SSEA-4 antibodies both before and after culture for 15 days. In conclusion, it may be possible to develop the testis-mediated germline chimera production technique, which extends the feasibility of genetic manipulations in avian species.</P>
< 전시-P-45 > Accelerated Weathering of Virgin WPC/Waste WPC: Mechanical and Morphological Properties
( Sang-u Bae ),( Young-rok Seo ),( Jae-gyoung Gwon ),( Sun-young Lee ),( Birm-june Kim ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1
Wood-plastic composites (WPCs) have attracted much attention in recent decades due to their competitive cost, great processability and excellent physical performance. Also, some of their main applications are represented by outdoor decking and furnishing. Since they are mainly applied to outdoor places, it is very important to evaluate the degradation behavior of WPCs under UV irradiation and moisture exposure, which are the major natural weathering factors. The chief aim of this research is to recycle waste-WPC(W-WPC) collected after use. However, such a W-WPC is inevitably deteriorated properties. In order to overcome these problems, virgin-WPC(V-WPC) was prepared with the same recipe as that of the original WPC. Five different blends were designed with different proportions of W-WPC and V-WPC. Each of the blends was prepared using extrusion and injection moulding, and then it was subjected to analyze various characterizations. The evaluation of weathering behavior by accelerated artificial weathering was carried out according to ASTM D2565 and total 1000 hours of exposure time was applied to test specimens using the Weather-O-Meter(WOM) equipment. Tensile, flexural properties, and impact strength of the specimens were tested at each 250, 500, and 1000 hours after accelerated weathering. The morphology of the weathered surface was observed by scanning electron microscope(SEM). From this study, a certain corelation between mechanical and morphological properties that occurred during accelerated weathering of W-WPC/V-WPC blends were established.