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Elicitation and In Situ Recovery of Alkaloids in suspension Cultures of California Poppy
Byun, Sang Yo,Pedersen, Henrik 한국미생물 · 생명공학회 1991 Journal of microbiology and biotechnology Vol.1 No.3
Large and rapid increases in benzophenanthridine alkaloid production occured in suspension cultures of Eschscholtzia californica cells treated with elicitors. Response to different biotic elicitors showed that elicitors prepared from yeast extract, Collectotrichum lindemuthianum and Verticillium dahliae induced alkaloid formation. Highest alkaloid accumulation was obtained with 60㎍ of yeast extract elicitor per gram of fresh cell weight. In time course performance after elicitor addition, more than 40 hours were required to obtain saturated alkaloid accumulation. Compounded silicone fluid, an ideal accumulation phase for two-phase culture of E. californica, accumulated a large amount of alkaloids produced in a specific manner. Elicitation in two-phase culture clearly increased net alkaloid production as well as their concentrations in the accumulation phase.
Re-Elicitation with Methyl Jasmonate in Eschscholtzia californica Cell Suspension Cultures
BYUN, SANG YO 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.1
Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elicitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250㎎/l, which was 2-fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.
A study on using acoustic emission in rock slope with difficult ground – focused on rainfall
Yo-Seph Byun,Myung Sagong,Suk-Chun Kim,Byung Sik Chun,Seong-Yong Park,Hyuk-Sang Jung 한국지질과학협의회 2012 Geosciences Journal Vol.16 No.4
This study attempted to predict slope failures by applying AE techniques to a rock slope with a history of collapse. Damage levels were assessed on the basis of the AE characteristics estimated by conducting laboratory tests on simulated slope failures. The test results showed that the specimen showed a very high degree of failure at the initial stage of loading, which was analyzed using the AE characteristics to assess the damage levels. The variation in the measured number of AE hits and AE events in field tests may indicate minute variations in the slope. The measured values appeared to be insignificant as they were less than the minimum criterion for damage. An analysis of rainfall, which is an important factor in slope behavior, showed that as rainfall increased, the number of AE hits and AE events also increased. In particular, when the cumulative rainfall was constant, the cumulative number of AE hits and AE events appeared to converge, indicating that the cumulative rainfall and the number of AE hits are closely connected. When applying AE techniques to a slope that has a high probability of failure, the failure prediction of the specific location and the early prediction of failure behavior on rock slopes became possible.
( Sang Eun Lee ),( Jung Min Lim ),( Hee Cheol Kang ),( Sang Yo Byun ),( Kyung Real Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Methods to measure the effectiveness ofcleansing products are few and not standardized. Objectives: To develop and compare a new test method to measure the efficacy of cleansing products using a dermoscopy image analysis of cyanoacrylate skin surface stripping (CSSS) and the existing methods including chromameter and mexameter. Methods: 25 subjects were enrolled. The cleaning efficacy of cleansing products was assessed against model dirt by measuring lightness using a chromameter and melanin index using a mexameter before and after wash. CSSS samples of the washed spot were imaged using a dermoscopy and analyzed by digital image analysis. Residual dirt was determined as the pixels numbers calculated for the area of the image and cleansing efficacy(%) was reported as 100-%area measured by Image J. Intraclass correlation coefficient (ICC) was used to evaluate the agreement between methods. The reproducibility of each method was also assessed. Results: The ICC between the cleansing efficacy obtained from a dermoscopy image analysis of CSSS and the existing methods (0.826 with chromameter and 0.731 with mexameter) was high (>0.6). The coefficients of variability of cleansing efficacy measured by a dermoscopy image analysis of CSSS, chromameter, and mexameter were 4.87, 14.34, and 17.86%, respectively. Conclusion: This new method shows high agreement with existing methods and shows a higher reproducibility than existing methods.
델파이 기법을 활용한 성능중심의 공동구 평가 항목 개발
변요셉 ( Byun Yo-seph ),성주현 ( Seong Joo-hyun ),서정은 ( Seo Jeong-eun ),박상필 ( Park Sang-pil ) 한국구조물진단유지관리공학회 2022 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.26 No.2
최근 기반시설관리법이 시행(’20.10)되면서 지하시설물 통합관리로 인한 공동구의 중요성이 높아진 실정이다. 공동구는 전기, 통신, 상수도, 난방 시설과 같은 필수적인 Life-Line을 지하에 공동 수용하는 기반시설물로서 대규모 토지개발사업과 신도시 개발 등에 따라 지속적으로 증가하고 있으나 공동구와 같은 지하시설물 특성상 개보수가 쉽지 않기 때문에 성능중심의 관리를 통한 효율적인 장수명화가 필요하다.