ON THE ENERGY OF MAXIMUM PRODUCTION OF INTERMEDIATE MASS FRAGMENTS IN HEAVY-ION COLLISIONS USING MICROSCOPIC MODEL

SAKSHI SHARMA,ROHIT KUMAR,RAJEEV K. PURI 장전수학회 2021 Proceedings of the Jangjeon mathematical society Vol.24 No.4

The present study is aimed to show the role played by impact parameter on the peak production of intermediate mass fragments (IMFs) in heavy-ion collisions. The study is conducted for both mass symmetric and mass asymmetric colliding nuclei. Here, the reactions are simulated using a Monte-Carlo based computer program developed utilizing the dynamical equations. Our present analysis shows that the impact parameter influences the reaction results on peak IMF production more drastically for mass asymmetric reactions than mass symmetric reactions.

Single Digit Hash Boyer Moore Horspool Pattern Matching Algorithm for Intrusion Detection System

Sakshi Sharma,Manish Dixit 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.9

During the past time information on internet increasing enormously which greed the attacker and invite them for attack. In order to provide protection from illegal access the concept of Intrusion Detection System (IDS) is introduced. Intrusion detection system recognized as a powerful tool for identifying malicious attacks over the network. IDS works on the concept of string matching with the help of Detection engine. Detection engine of IDS uses String matching algorithm for comparing against malicious activities. This comparison takes enough processing time nearly 70% of the whole IDS processing time by improving performance of searching algorithm. In this paper, we proposed an enhanced version of Hash-Boyer-Moore-Horspool string matching algorithm by adding a single digit hash function for reduced the number of character comparisons and improve the efficiency of IDS by reducing the number of false positive match.

A Review on Network Intrusion Detection System Using Open Source Snort

Sakshi Sharma,Manish Dixit 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.4

In the present scenario most of the organization depends on the internet for their communication, storage and protection of their valuable data and other internal resources from the unauthorized access as the importance of internet is increasing rapidly the chances of attacks also increases in the ratio. Intrusion Detection System plays a very important role in network security. Its main role in the network is to help computer system to create and deal with network attacks. An IDS acts as a key component to ensuring the safety of any network or system on which it runs. IDS works on the concept of investigating all the incoming packets for the detection of any malicious activity. This is a survey paper on the various enhancements over the decades on IDS. Snort is a lightweight and open source software which used signature based IDS. In the survey, we used BASE for providing graphical interface for displaying the result. Its used world widely in Intrusion Detection and Prevention.

Composition and Diversity of Gut Bacteria Associated with the Eri Silk Moth, Samia ricini, (Lepidoptera: Saturniidae) as Revealed by Culture-Dependent and Metagenomics Analysis

Kondwani MsangoSoko,Sakshi Gandotra,Rahul Kumar Chandel,Kirti Sharma,Balasubramanian Ramakrishinan,Sabtharishi Subramanian 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.9

The polyphagous eri silk moth, Samia ricini, is associated with various symbiotic gut bacteria believed to provide several benefits to the host. The larvae of S. ricini were subjected to isolation of gut bacteria using culture-dependent 16S rRNA generic characterization, metagenomics analysis and qualitative enzymatic assays. Sixty culturable aerobic gut bacterial isolates comprising Firmicutes (54%) and Proteobacteria (46%); and twelve culturable facultative anaerobic bacteria comprising Proteobacteria (92%) and Firmicutes (8%) were identified inhabiting the gut of S. ricini. The results of metagenomics analysis revealed the presence of a diverse community of both culturable and un-culturable gut bacteria belonging to Proteobacteria (60%) and Firmicutes (20%) associated with seven orders. An analysis of the results of culturable isolation indicates that these bacterial isolates inhabited all the three compartments of the gut. Investigation on persistence of bacteria coupled with metagenomics analysis of the fifth instar suggested that bacteria persist in the gut across the different instar stages. In addition, enzymatic assays indicated that 48 and 75% of culturable aerobic, and 75% of anaerobic gut bacterial isolates had cellulolytic, lipolytic and nitrate reductase activities, thus suggesting that they may be involved in food digestion and nutritional provision to the host. These bacterial isolates may be good sources for profiling novel genes and biomolecules for biotechnological application.

Vimentin as a potential therapeutic target in sorafenib resistant HepG2, a HCC model cell line

Ankita Makol,Harpreet Kaur,Sakshi Sharma,Shruthi Kanthaje,Ramanpreet Kaur,Anuradha Chakraborti 대한간학회 2020 Clinical and Molecular Hepatology(대한간학회지) Vol.26 No.1

Background/Aims: Hepatocellular carcinoma (HCC) is the most common liver cancer with high mortality rate in patients suffering from liver diseases. The drug of choice used in advanced-stage of HCC is sorafenib. However, adaptive resistance has been observed in HCC patients undergoing long-term sorafenib treatment, lowering its effectiveness. Hence, it is important to overcome drug resistance to improve overall management of HCC. Here, we have identified a candidate biomarker for sorafenib resistance in a HCC model cell line, HepG2. Methods: Initially, comparative proteomic profiling of parental HepG2 [HepG2 (P)] and sorafenib-resistant HepG2 [HepG2 (R)] cells was performed via MALDI (matrix-assisted laser desorption/ionization) which revealed the deregulation of vimentin in HepG2 (R) cells. Gene and protein level expression of vimentin was also observed through quantitative real-time polymerase chain reaction (qRT PCR) and fluorescence-activated cell sorting (FACS), respectively. Furthermore, withaferin A was used to study regulation of vimentin expression and its significance in sorafenib resistance. Results: Both gene and protein level of vimentin expression was found to be downregulated in HepG2 (R) in comparison to HepG2 (P). Interestingly, the study demonstrated that withaferin A further lowered the expression of vimentin in HepG2 (R) cells in a dose-dependent manner. Also, inhibition of vimentin lowered ABCG2 expression and decreased cell viability in parental as well as sorafenib resistant HepG2 cells. Conclusions: Hence, our study for the first time highlighted the probable therapeutic potential of vimentin in sorafenib resistant HepG2, a HCC model cell line.

Screening and characterization of lipase producing bacteria isolated from the gut of a lepidopteran larvae, Samia ricini

MsangoSoko Kondwani,Gandotra Sakshi,Bhattacharya Ramcharan,Ramakrishnan Balasubramanian,Sharma Kirti,Subramanian Sabtharishi 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

Lipolytic enzymes are an important group of hydrolases that have found immense industrial application in biotechnology. In this study, the ability of gut bacteria isolated from the gut of the Eri silkworm, Samia ricini, to produce lipolytic enzymes was evaluated through qualitative and quantitative assays. The results of lipase screening showed that 28 isolates had lipolytic activity. The results of 16S ribosomal RNA sequencing indicated that the genus Bacillus comprised majority of the lipolytic bacterial isolates (71%) followed by Pseudomonas (15%); whilst Acinetobacter, Enterobacter and Enterococcus comprised 11%. Lipolytic activity was found in bac teria isolates identified from all the three gut compartments of S. ricini larvae with significant activity from isolates extracted from the foregut and midgut. The lipolytic index among the bacterial isolates ranged between 0.63 and 2.81 on Rhodamine B medium, and all isolates exhibited significant lipolytic activity with p-nitrophenyl butyrate (PNPB) with specific activity ranging from 0.52 to 0.82 μmol/min/mg. The effect of pH and temperature showed that lipase activity was optimum at 37 ◦ C and pH 7–9. A phylogenetic relationship of lipase producing gut bacteria indicated high cluster stability for isolates from different stages (>50%) suggesting that the isolates persist across developmental stages of the host. The Eri silkworm is reared for its silk and the knowledge of its gut bacteria with the ability to produce lipases lies in the significance as far as boosting production of this insect via development of probiotics to enhance commercial Eri rearing. In addition, this insect may be a good resource for profiling novel lipolytic microbes for commercial production of lipases as lipases from microbial origin have assumed a great deal of importance as industrial enzymes due to their potential for use in biotechnology.