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      • SCIESCOPUSKCI등재

        Chemical Composition and Insecticidal Activity of Artemisia scoparia Essential Oil against Three Coleopteran Stored-Product Insects

        Negahban, Maryam,Moharramipour, Saeid,Sefidkon, Fatemeh Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.4

        Chemical composition of the essential oil from Artemisia scoparia Waldst et Kit, and its fumigant and repellent activity were investigated against three stored product insects, Callosobruchus maculatus (Fab.), Sitophilus oryzae (L.), and Tribolium castaneum (Herbst). Dry ground leaves were subjected to hydrodistillation using a modified clevenger-type apparatus and the chemical composition of the volatile oil was studied by GC-MS. Nineteen components (99.51 % of the total composition) were identified. ${\beta}-Pinene$ (19.01%), capillin (17.45%), limonene (15.11 %), myrcene (10.95) were found to be the major constituents of the oil. The mortality of 1-7 day old adults of the insect pests increased with concentration from 37 to $926{\mu}L$ per L air and with exposure time from 3 to 24 h. A concentration of $37{\mu}L$ per L air and exposure time of 24 h was sufficient to obtain 100% kill of the insects. Callosobruchus maculatus was more susceptible than S. oryzae and T. castaneum. A second more detailed bioassay gave estimates for the $LC_{50}$ of C. maculatus as $1.46{\mu}L$ per Lair, S. oryzae $1.87{\mu}L$ per Lair and T. castaneum $2.05{\mu}L$ per L air. Also, the essential oil was significantly more repellent to T. castaneum and S. oryzae than C. maculatus. However, half-life time of the oil for C. maculatus was longer than S. oryzae and T. castaneum. These results show the efficacy of A. scoparia oil for use in organic food protection.

      • KCI등재

        The synergistic effects of Carum copticum essential oil on diatomaceous earth against Sitophilus granarius and Tribolium confusum

        Masumeh Ziaee,Saeid Moharramipour,Jacek Francikowski 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.4

        In this study, synergistic/antagonistic interaction between Carum copticum (L.) powder and essential oil with diatomaceousearths (DEs) was assessed against Tribolium confusum Jacquelin du Val. and Sitophilus granarius (L.)adults. The obtained synergistic interactionwas interpreted by measurement of insects' locomotor activity whenexposed to C. copticum essential oil. Essential oil had strong fumigant toxicity against adult insects; however, toxicitydiminished in the presence of wheat commodity. Plant essential oil synergized the performance of DE samplessuch that they generally became more insecticidal than DE alone. However, plant powder effects wereantagonistic; effects were not as severe as predicted in terms of the insect mortality. Insects' locomotor activityincreased significantlywhen exposed to C. copticumessential oil. These results support the hypothesis that essentialoil can enhance the efficacy of DE, at least by increasing locomotor activity and insect-contact toxicity of DEparticles. Therefore, combination of essential oil with DE could have potential for use in integrated storedproductpest-management programs.

      • SCIESCOPUSKCI등재

        Seasonal Changes of Fatty Acid Compositions in Overwintering Larvae of Rice Stem Borer, Chilo suppressalis (Lepidoptera: Pyralidae)

        Atapour, Maryam,Moharramipour, Saeid,Barzegar, Mohsen Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.1

        Cold acclimation and overwintering state can affect fatty acid compositions of insects. To determine compositional change of fatty acids during nondiapause and diapause stages, an experiment was conducted to investigate fatty acid constituents from whole body of C. suppressalis larvae. Five most abundant fatty acids were found to be palmitoleic (35-58%), palmitic (18-44%), oleic (14-23%), stearic (0.5-2.5%) and linoleic acid (0.4-2%). However, linolenic, erucic, lauric and myristic acid were found at lower level. Saturated fatty acids significantly decreased and conversely unsaturated fatty acids increased from August (pre-diapause) to October (initiation of diapause). The increase in seasonal cold hardiness during cold acclimation, exposed at $-15^{\circ}C$ for 24 h, was related to degree of fatty acid un-saturation. The elevation of palmitoleic acid content at low temperature resulted in an increase in the overall degree of unsaturation in the whole body. These results indicated the importance of unsaturated fatty acids composition to prepare larvae entering diapause phase.

      • KCI등재

        Seasonal Changes of Fatty Acid Compositions in Overwintering Larvae of Rice Stem Borer, Chilo suppressalis (Lepidoptera: Pyralidae)

        Maryam Atapour,Saeid Moharramipour,Mohsen Barzegar 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.1

        Cold acclimation and overwintering state can affect fatty acid compositions of insects. To determine compositional change of fatty acids during non- diapause and diapause stages, an experiment was conducted to investigate fatty acid constituents from whole body of C. suppressalis larvae. Five most abundant fatty acids were found to be palmitoleic (35-58%), palmitic (18-44%), oleic (14-23%), stearic (0.5-2.5%) and linoleic acid (0.4-2%). However, linolenic, erucic, lauric and myristic acid were found at lower level. Saturated fatty acids significantly decreased and conversely unsaturated fatty acids increased from August (pre-diapause) to October (initiation of diapause). The increase in seasonal cold hardiness during cold acclimation, exposed at -15℃ for 24 h, was related to degree of fatty acid unsaturation. The elevation of palmitoleic acid content at low temperature resulted in an increase in the overall degree of unsaturation in the whole body. These results indicated the importance of unsaturated fatty acids composition to prepare larvae entering diapause phase.

      • SCIESCOPUSKCI등재

        Insecticidal Activity and Chemical Composition of Artemisia sieberi Besser Essential Oil from Karaj, Iran

        Negahban, Maryam,Moharramipour, Saeid,Sefidkon, Fatemeh Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.1

        Atremisia sieberi Besser is a widely distributed plant that grows in many areas of Iran and has strong insecticidal activity against stored product pests, so an experiment was conducted to investigate fumigant toxicity of the A. sieberi oil collected from Karaj region of Iran. The oil was applied against one to seven day old adults of three major stored product insects including: Callosobruchus maculatus (Fab.), Sitophilus oryzae (L.), and Tribollium castaneum (Herbst). The potency of fumigant toxicity of A. sieberi on C. maculatus was higher $(LC_{50}:\;1.64{\mu}L\;per\;L)$ than S. oryzae $(LC_{50}:\;4.41{\mu}L\;per\;L)$ and T. castaneum $(LC_{50}:\;20.31{\mu}L\;per\;L)$ L). The relationships between the time exposure and oil concentration on mortality show that the mortality was increased as oil concentration and exposure time was increased. The concentration of $185{\mu}L$ per L and exposure time of 24h was enough to obtain 100% kill of the insects. It was also found that the regions where A. sieberi grows affect essential oil components of the plant and can play an important role in properties of fumigant toxicity.

      • KCI등재

        Chemical Composition and Insecticidal Activity of Artemisia scoparia Essential Oil against Three Coleopteran Stored-Product Insects

        Maryam Negahban,Saeid Moharramipour,Fatemeh Sefidkon 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.4

        Chemical composition of the essential oil from Artemisia scoparia Waldst et Kit, and its fumigant and repellent activity were investigated against three stored product insects, Callosobruchus maculatus (Fab.), Sitophilus oryzae (L.), and Tribolium castaneum (Herbst). Dry ground leaves were subjected to hydrodistillation using a modified clevenger-type apparatus and the chemical composition of the volatile oil was studied by GC-MS. Nineteen components (99.51% of the total composition) were identified. β-Pinene (19.01%), capillin (17.45%), limonene (15.11%), myrcene (10.95) were found to be the major constituents of the oil. The mortality of 1-7 day old adults of the insect pests increased with concentration from 37 to 926 μL per L air and with exposure time from 3 to 24 h. A concentration of 37 μL per L air and exposure time of 24 h was sufficient to obtain 100% kill of the insects. Callosobruchus maculatus was more susceptible than S. oryzae and T. castaneum. A second more detailed bioassay gave estimates for the LC50 of C. maculatus as 1.46 μL per L air, S. oryzae 1.87 μL per L air and T. castaneum 2.05 μL per L air. Also, the essential oil was significantly more repellent to T. castaneum and S. oryzae than C. maculatus. However, half-life time of the oil for C. maculatus was longer than S. oryzae and T. castaneum. These results show the efficacy of A. scoparia oil for use in organic food protection.

      • KCI등재

        Insecticidal Activity and Chemical Composition of Artemisia sieberi Besser Essential Oil from Karaj, Iran

        Maryam Negahban,Saeid Moharramipour,Fatemeh Sefidkon 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.1

        sieberiBesser is a widely dis-tributed plant that grows in many areas of Iran andhas trong insecticidal activity against stored productpests, so an experiment was conducted to investigatefumigant toxicity of theA. sieberioil collected fromKaraj region of Iran. The oil was applied againstone to seven day old adults of three major storedproduct insects including:Callosobruchus maculatus(Fab.),Sitophilus oryzae(L.), andTribollium cas-taneum(Herbst). The potency of umigant oxicityof A. sieberion C. maculatuswas higher (LC50:1.64 L per L) thanμ S. oryzae(LC50: 4 . 4 1 L p e rμL) andT. castaneum(LC50: 20.31 L per L). Theμrelationships between the time xposure and oilconcentration on mortality show that the mortalitywas increased as oil concentration and exposure timewas increased. The concentration of 185 L per Lμand exposure time of 24h was enough to obtain100% kill of the insects. It was also found that theregions whereA. sieberigrows affect essential oilcomponents of the plant and can play an importantKey words Artemisia sieberi, fumigant toxicity,botanical insecticides, stored product insects, che-motype

      • KCI등재

        Insecticidal activity of Artemisia sieberi, Eucalyptus camaldulensis, Thymus persicus and Eruca sativa oils against German cockroach, Blattella germanica (L.)

        Mehran Rezaei,Ramin Khaghani,Saeid Moharramipour 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4

        The German cockroach, Blattella germanica (L.) (Blattodea: Blattellidae), has been recognized as a serious health problem because of its potential to harbor and transmit human disease-causing pathogens. The development of new and environmentally friendly control agents is essential as alternative tool to conventional chemical insecticides. The aim of the current study was to determine the fumigant and contact toxicity of essential oils (EOs), Artemisia sieberi Besser, Eucalyptus camaldulensis Dehn. and Thymus persicus (Ronniger ex Rech. f.) and cold press oil of Eruca sativa (Miller) (ESO) against first instar nymphs and adults. According to results, the LC 50 values of EOs were between 15.01 and 28.80 μl/l air for fumigant toxicity, 2202.8 and 5600.2 mg/l for dipping toxicity and 9484.2 and 34,421.1 mg/l for applied surface toxicity. Fumigant activity of the oils were significantly more toxic than other methods. In general, the toxicity of A. sieberi was higher than other EOs. Also, the first instar nymphs were more susceptible than adults. Additionally, the results showed that the EOs were significantly more toxic than ESO in both fumigant and dipping methods. In addition to contact effects, the fumigant activity of ESO was proved, although the toxicity was lower than the EOs. Hence, it is important to consider the production cost benefit of ESO in comparison with the EOs. So, the ESO could be considered as a good candidate in control of the cockroach but in enclosed spaces such as inaccessible cockroach hiding places, the EO of A. sieberi can be useful.

      • KCI등재

        Fumigant toxicity of essential oil from Vitex pseudo-negundo against Tribolium castaneum (Herbst) and Sitophilus oryzae (L.)

        Bibi Zahra Sahaf,Saeid Moharramipour,Mohammad Hadi Meshkatalsadat 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.4

        The objective of the current study was to determine the chemical constituents and fumigant toxicity of an essential oil that was isolated via hydrodistillation from dry leaves of Vitex pseudo-negundo (Hausskn.) Hand.-Mzz. The chemical composition of the essential oil was assessed via GC and GC-MS. 1, 8-Cineol (18.23%), α-Pinene (16.20%) and Sabinene (5.67%) were determined to be the major constituents of the oil. The fumigant toxicity of the essential oil was tested against 1–7 day-old adults of Tribolium castaneum (Herbst) and Sitophilus oryzae (L.) at 27±1 °C and 60±5% r.h. in darkness. The mortality of adults was tested at different concentrations ranging from 37.0 to 925.9 μL/L air and different exposure times (1–30 h). The results demonstrated that the mortality increased with increases in concentration and exposure time. At concentrations higher than 185.2 μL/L air, the mortality was recorded at more than 50% after 10 h, and reached 100% after 12–16 h. Data probit analysis demonstrated that S. oryzae (LC50=31.96 μL/L air) was more susceptible than T. castaneum (LC50= 47.27 μL/L air). These results showed that the essential oil from V. pseudo-negundo could be applicable to the management of populations of stored-product insects. The objective of the current study was to determine the chemical constituents and fumigant toxicity of an essential oil that was isolated via hydrodistillation from dry leaves of Vitex pseudo-negundo (Hausskn.) Hand.-Mzz. The chemical composition of the essential oil was assessed via GC and GC-MS. 1, 8-Cineol (18.23%), α-Pinene (16.20%) and Sabinene (5.67%) were determined to be the major constituents of the oil. The fumigant toxicity of the essential oil was tested against 1–7 day-old adults of Tribolium castaneum (Herbst) and Sitophilus oryzae (L.) at 27±1 °C and 60±5% r.h. in darkness. The mortality of adults was tested at different concentrations ranging from 37.0 to 925.9 μL/L air and different exposure times (1–30 h). The results demonstrated that the mortality increased with increases in concentration and exposure time. At concentrations higher than 185.2 μL/L air, the mortality was recorded at more than 50% after 10 h, and reached 100% after 12–16 h. Data probit analysis demonstrated that S. oryzae (LC50=31.96 μL/L air) was more susceptible than T. castaneum (LC50= 47.27 μL/L air). These results showed that the essential oil from V. pseudo-negundo could be applicable to the management of populations of stored-product insects.

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