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기술 교육에 있어서 학습 장소에 따른 교수 방법의 효율성에 관한 이론적 고찰
권현진,류창열 충남대학교 공업교육연구소 2000 論文集 Vol.23 No.1
This study is to find the effective ways of achieving learning goals by applying appropriate teaching methods in various learning conditions. The study areas are as follows: 1. surveying various characteristics in accordance to the learning places which affect teaching methods, 2. investigating teaching methods suitable to the industrial education and workplaces, 3. researching the teaching method of the school in detail. This study is adopted literature review and analytic research in order to obtain the object. The major findings of this study are as follows: 1. The learning place can be divided into the workplace and school because of the characteristic of technology education. We should try to use these places effectively. 2. In the workplace, several teaching methods like as work observation, general work experience program, work-study, cooperative education system, internship are applied to general education and vocational education 3. Teaching methods of school are classified with the specific property of learning contents, the learning purpose, the ability of student and so on.
Jeon, Hyunjin,Lee, Euiyeon,Kim, Dahee,Lee, Minhyung,Ryu, Jeahee,Kang, Chungwon,Kim, Soyoun,Kwon, Youngeun American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.16
<P>Live-cell-based biosensors have emerged as a useful tool for biotechnology and chemical biology. Genetically encoded sensor cells often use bimolecular fluorescence complementation or fluorescence resonance energy transfer to build a reporter unit that suffers from nonspecific signal activation at high concentrations. Here, we designed genetically encoded sensor cells that can report the presence of biologically active molecules via fluorescence-translocation based on split intein-mediated conditional protein trans-splicing (PTS) and conditional protein trans-cleavage (PTC) reactions. In this work, the target molecules or the external stimuli activated intein-mediated reactions, which resulted in activation of the fluorophore-conjugated signal peptide. This approach fully valued the bond-making and bond-breaking features of intein-mediated reactions in sensor construction and thus eliminated the interference of false-positive signals resulting from the mere binding of fragmented reporters. We could also avoid the necessity of designing split reporters to refold into active structures upon reconstitution. These live-cell-based sensors were able to detect biologically active signaling molecules, such as Ca<SUP>2+</SUP> and cortisol, as well as relevant biological stimuli, such as histamine-induced Ca<SUP>2+</SUP> stimuli and the glucocorticoid receptor agonist, dexamethasone. These live-cell-based sensing systems hold large potential for applications such as drug screening and toxicology studies, which require functional information about targets.</P> [FIG OMISSION]</BR>
Yoon, Hyunjin,Yun, Jiae,Lim, Jeong-A,Roh, Eunjung,Jung, Kyu-Seok,Chang, Yoonjee,Ryu, Sangryeol,Heu, Sunggi Society for General Microbiology 2013 The Journal of general virology Vol.94 No.11
<P><I>Staphylococcus aureus</I> is one of the most important pathogens, causing various diseases in humans and animals. As methicillin-resistant <I>S. aureus</I> (MRSA) has become increasingly prevalent, controlling this pathogen with standard antibiotic treatment has become challenging. Bacteriophages (phages) have attracted interest as alternative antibacterial agents to control MRSA. In this study, we isolated six <I>S. aureus</I> phages from soils of poultry/livestock farms. Based on the results of host range determination with 150 <I>S. aureus</I> strains and restriction enzyme treatment of phage DNA, two phages, designated SP5 and SP6, were selected for further characterization and genome sequencing. Both SP5 and SP6 were classified as members of the family <I>Siphoviridae</I>. The genome of SP5 comprises 43 305 bp and contains 63 ORFs, while the SP6 genome comprises 42 902 bp and contains 61 ORFs. Although they have different host spectra, the phage genomes exhibit high nucleotide similarity to each other. Adsorption assay results suggested that the host range determinants of the two phages are involved in both adsorption and infection. Comparative genomic analyses of the two phages provided evidence that the lysogenic/lytic control module and tail proteins may be important for host specificity.</P>
Noh, Hyunjin,Kim, Hyun J,Yu, Mi R,Kim, Wan-Young,Kim, Jin,Ryu, Jung H,Kwon, Soon H,Jeon, Jin S,Han, Dong C,Ziyadeh, Fuad United States and Canadian Academy of Pathology [e 2012 Laboratory investigation Vol.92 No.11
<P>The accumulation of extracellular matrix proteins in the interstitial area is the final common feature of chronic kidney diseases. Accumulating evidence suggests that transforming growth factor (TGF)-관1 promotes the development of renal fibrosis. Heat shock protein (Hsp) 90 inhibitors have been shown to repress TGF-관1 signaling, but whether they inhibit renal fibrosis is unknown. The purpose of this study is to determine the therapeutic efficacy of Hsp90 inhibitor on renal fibrosis. In TGF-관1-treated HK2 cells and unilateral ureteral obstruction (UUO) kidneys, we found that 17-allylamino-17-demethoxygeldanamycin (17AAG), an Hsp90 inhibitor, decreased the expression of 관-smooth muscle actin, fibronectin, and collagen I and largely restored the expression of E-cadherin. 17AAG inhibited TGF-관1-mediated phosphorylation of Smad2, Akt, glycogen synthase kinase-3관, and extracellular signal-regulated kinase in HK2 cells. Inhibition of Hsp90 also blocked TGF-관1-mediated induction of snail1. This 17AAG-induced reduction was completely restored by simultaneous treatment with proteasome inhibitor MG132. Furthermore, 17AAG blocked the interaction between Hsp90 and TGF-관 type II receptor (T관RII) and promoted ubiquitination of T관RII, leading to the decreased availability of T관RII. Smurf2-specific siRNA reversed the ability of 17AAG to inhibit TGF-관1 signaling. The effect of 17AAG on T관RII expression and renal fibrosis was confirmed in UUO kidneys. These findings suggest that Hsp90 inhibitor prevents the development of renal fibrosis via a mechanism dependent on Smurf2-mediated degradation of T관RII.</P>
톳 열수 추출물이 마우스 비장세포 증식증과 염증성 사이토카인 (IL-1β, IL-6, TNF-α) 분비에 미치는 영향
박현진(Hyunjin Park),류혜숙(HyeSook Ryu) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.12
Hizikia fusiforme (seaweed fusiforme) has long been used as a food source mainly in Korea and Japan. This study was performed to evaluate the immunomodulative effects of Hizikia fusiforme in mice. Hizikia fusiforme water extracts (0, 50, and 500 mg/kg b.w.) were orally administrated into the mice every other day, for four weeks. The proliferation of splenocytes, as well as the levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) secreted by activated macrophages were measured. Splenocyte proliferation was enhanced in the experimental groups compared to that of the control group. Also, the mice with Hizikia fusiforme water extracts supplementation in both concentrations showed increased levels of cytokine production by activated peritoneal macrophages compared to those in the control group. The highest levels of cytokine (IL-1β, IL-6, TNF-α) production were observed in the 50 mg/kg b.w. supplementation group stimulated by LPS for all three cytokines. The results of this study showed that the supplementation of Hizikia fusiforme water extracts may enhance the immune function by regulating the splenocytes proliferation and the cytokine production by activated macrophages. Further studies are needed to identify the stimulative and immunomodulating components of Hizikia fusiforme.