A Bacterium Belonging to the Burkholderia cepacia Complex Associated with Pleurotus ostreatus

Ricardo Yara,Walter Maccheroni Jr,Jorge Horii,Joao Lucio Azevedo 한국미생물학회 2006 The journal of microbiology Vol.44 No.3

Pleurotus ostreatus is a widely cultivated white-rot fungus. Owing to its considerable enzymatic versatility P. ostreatus has become the focus of increasing attention for its possible utility in biobleaching and bioremediation applications. Interactions between microorganisms can be an important factor in those processes. In this study, we describe the presence of a bacterial species associated with P. ostreatus strain G2. This bacterial species grew slowly (approximately 30 days) in the liquid and semi-solid media tested. When P. ostreatus was inoculated in solid media containing Tween 80 or Tween 20, bacterial microcolonies were detected proximal to the fungal colonies, and the relevant bacterium was identified via the analysis of a partial 16S rDNA sequence; it was determined to belong to the Burkholderia cepacia complex, but was not closely related to other fungus-isolated Burkholderiaceae. New specific primers were designed, and confirmed the presence of in vitro P. ostreatus cultures. This is the first time that a bacterial species belonging to the B. cepacia complex has been found associated with P. ostreatus.

A Bacterium Belonging to the Burkholderia cepacia Complex Associated with Pleurotus ostreatus

Yara Ricardo,Maccheroni Junior Walter,Horii Jorge,Azevedo Joao Lucio The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.3

Pleurotus ostreatus is a widely cultivated white-rot fungus. Owing to its considerable enzymatic versatility p. ostreatus has become the focus of increasing attention for its possible utility in biobleaching and bioremediation applications. Interactions between microorganisms can be an important factor in those processes. In this study, we describe the presence of a bacterial species associated with P. ostreatus strain G2. This bacterial species grew slowly (approximately 30 days) in the liquid and semi-solid media tested. When p. ostreatus was inoculated in solid media containing Tween 80 or Tween 20, bacterial microcolonies were detected proximal to the fungal colonies, and the relevant bacterium was identified via the analysis of a partial 16S rDNA sequence; it was determined to belong to the Burkholderia cepacia complex, but was not closely related to other fungus-isolated Burkholderiaceae. New specific primers were designed, and confirmed the presence of in vitro P. ostreatus cultures. This is the first time that a bacterial species belonging to the B. cepacia complex has been found associated with P. ostreatus.

Effects of endodontic tri-antibiotic paste on bond strengths of dentin adhesives to coronal dentin

Mirzakoucheki, Parvin,Walter, Ricardo,Khalighinejad, Navid,Jahromi, Maryam Zare,Mirsattari, Sanaz,Akbarzadeh, Navid The Korean Academy of Conservative Dentistry 2015 Restorative Dentistry & Endodontics Vol.40 No.2

Objectives: The aim of this study was to evaluate the effects of tri-antibiotic paste (TAP) on microtensile bond strengths (MTBS) of dental adhesives to dentin. Materials and Methods: Sixty extracted molars had their occlusal surfaces flattened to expose dentin. They were divided into two groups, i.e., control group with no dentin treatment and experimental group with dentin treatment with TAP. After 10 days, specimens were bonded using self-etch (Filtek P90 adhesive) or etch-and-rinse (Adper Single Bond Plus) adhesives and restored with composite resin. Teeth were sectioned into beams, and the specimens were subjected to MTBS test. Data were analyzed using two-way ANOVA and post hoc Tukey tests. Results: There was a statistically significant interaction between dentin treatment and adhesive on MTBS to coronal dentin (p = 0.003). Despite a trend towards worse MTBS being noticed in the experimental groups, TAP application showed no significant effect on MTBS (p = 0.064). Conclusions: The etch-and-rinse adhesive Adper Single Bond Plus presented higher mean bond strengths than the self-etch adhesive Filtek P90, irrespective of the group. The superior bond performance for Adper Single Bond when compared to Filtek P90 adhesive was confirmed by a fewer number of adhesive failures. The influence of TAP in bond strength is insignificant.

Effects of endodontic tri-antibiotic paste on bond strengths of dentin adhesives to coronal dentin

Parvin Mirzakoucheki,Ricardo Walter,Navid Khalighinejad,Maryam Zare Jahromi,Sanaz Mirsattari,Navid Akbarzadeh 대한치과보존학회 2015 Restorative Dentistry & Endodontics Vol.40 No.2

Objectives: The aim of this study was to evaluate the effects of tri-antibiotic paste (TAP) on microtensile bond strengths (MTBS) of dental adhesives to dentin. Materials and Methods: Sixty extracted molars had their occlusal surfaces flattened to expose dentin. They were divided into two groups, i.e., control group with no dentin treatment and experimental group with dentin treatment with TAP. After 10 days, specimens were bonded using self-etch (Filtek P90 adhesive) or etch-and-rinse (Adper Single Bond Plus) adhesives and restored with composite resin. Teeth were sectioned into beams, and the specimens were subjected to MTBS test. Data were analyzed using two-way ANOVA and post hoc Tukey tests. Results: There was a statistically significant interaction between dentin treatment and adhesive on MTBS to coronal dentin (p = 0.003). Despite a trend towards worse MTBS being noticed in the experimental groups, TAP application showed no significant effect on MTBS (p = 0.064). Conclusions: The etch-and-rinse adhesive Adper Single Bond Plus presented higher mean bond strengths than the self-etch adhesive Filtek P90, irrespective of the group. The superior bond performance for Adper Single Bond when compared to Filtek P90 adhesive was confirmed by a fewer number of adhesive failures. The influence of TAP in bond strength is insignificant.

A taxonomic account of non-geniculate coralline algae (Corallinophycidae, Rhodophyta) from shallow reefs of the Abrolhos Bank, Brazil

Michel B. Jesionek,Ricardo G. Bahia,Jazmín J. Hernández-Kantún,Walter H. Adey,Yocie Yoneshigue-Valentin,Leila L. Longo,Gilberto M. Amado-Filho 한국조류학회I 2016 ALGAE Vol.31 No.4

The Abrolhos Continental Shelf (ACS) encompasses the largest and richest coral reefs in the southern Atlantic Ocean. A taxonomic study of non-geniculate coralline algae (NGCA) from the region was undertaken using both morpho-anatomicaland molecular data. Specimens of NGCA were collected in 2012 and 2014 from shallow reefs of the ACS. Phylogeneticanalysis was performed using dataset of psbA DNA sequences from 16 specimens collected in the ACS and additionalGenBank sequences of related NGCA species. Nine common tropical reef-building NGCA species were identifiedand described: Hydrolithon boergesenii, Lithophyllum kaiseri, Lithophyllum sp., Lithothamnion crispatum, Melyvonneaerubescens, Pneophyllum conicum, Porolithon onkodes, Sporolithon ptychoides, and Titanoderma prototypum. A key forspecies identification is also provided in this study. Our molecular phylogenetic analyses suggest that Lithophyllum sp. corresponds to a new species. Our study also confirms that Lithophyllum kaiseri is a new record in Brazil. The psbA sequencesof Lithophyllum kaiseri and Melyvonnea erubescens matched with type specimens indirectly. The taxonomicidentification of the remaining species was supported by morpho-anatomical evidences as DNA sequences of their typesor topotypes remain unavailable.

A taxonomic account of non-geniculate coralline algae (Corallinophycidae, Rhodophyta) from shallow reefs of the Abrolhos Bank, Brazil

Jesionek, Michel B.,Bahia, Ricardo G.,Hernandez-Kantun, Jazmin J.,Adey, Walter H.,Yoneshigue-Valentin, Yocie,Longo, Leila L.,Amado-Filho, Gilberto M. The Korean Society of Phycology 2016 ALGAE Vol.31 No.4

The Abrolhos Continental Shelf (ACS) encompasses the largest and richest coral reefs in the southern Atlantic Ocean. A taxonomic study of non-geniculate coralline algae (NGCA) from the region was undertaken using both morpho-anatomical and molecular data. Specimens of NGCA were collected in 2012 and 2014 from shallow reefs of the ACS. Phylogenetic analysis was performed using dataset of psbA DNA sequences from 16 specimens collected in the ACS and additional GenBank sequences of related NGCA species. Nine common tropical reef-building NGCA species were identified and described: Hydrolithon boergesenii, Lithophyllum kaiseri, Lithophyllum sp., Lithothamnion crispatum, Melyvonnea erubescens, Pneophyllum conicum, Porolithon onkodes, Sporolithon ptychoides, and Titanoderma prototypum. A key for species identification is also provided in this study. Our molecular phylogenetic analyses suggest that Lithophyllum sp. corresponds to a new species. Our study also confirms that Lithophyllum kaiseri is a new record in Brazil. The psbA sequences of Lithophyllum kaiseri and Melyvonnea erubescens matched with type specimens indirectly. The taxonomic identification of the remaining species was supported by morpho-anatomical evidences as DNA sequences of their types or topotypes remain unavailable.