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DNA nanotechnology: a future perspective
Zahid, Muniza,Kim, Byeonghoon,Hussain, Rafaqat,Amin, Rashid,Park, Sung Ha Springer 2013 Nanoscale research letters Vol.8 No.1
<P>In addition to its genetic function, DNA is one of the most distinct and smart self-assembling nanomaterials. DNA nanotechnology exploits the predictable self-assembly of DNA oligonucleotides to design and assemble innovative and highly discrete nanostructures. Highly ordered DNA motifs are capable of providing an ultra-fine framework for the next generation of nanofabrications. The majority of these applications are based upon the complementarity of DNA base pairing: adenine with thymine, and guanine with cytosine. DNA provides an intelligent route for the creation of nanoarchitectures with programmable and predictable patterns. DNA strands twist along one helix for a number of bases before switching to the other helix by passing through a crossover junction. The association of two crossovers keeps the helices parallel and holds them tightly together, allowing the assembly of bigger structures. Because of the DNA molecule's unique and novel characteristics, it can easily be applied in a vast variety of multidisciplinary research areas like biomedicine, computer science, nano/optoelectronics, and bionanotechnology.</P>
IN VITRO THROMBIN DOSE RESPONSE ON MADIN DARBY CANINE KIDNEY CELL MONOLAYER
RASHID AMIN,IMRAN SHAKIR,ISHRAT SULTANA,박성하,RAFAQAT HUSSAIN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.4
Epithelial cells are known to play an important role in sustaining the airway barrier that may be impaired in certain inflammatory conditions. Recently, the use of thrombin has been reported to open the airway of patients with asthma as well as enhance the permeability of endothelial cell monolayers. We designed an in vitro model of Madin Darby Canine Kidney (MDCK) cells and the physiological functions of this model were evaluated by measuring the transepithelial resistance (TER). The epithelial cytoskeletal organization was observed by staining with Bisbenzimide and Rodamine-Phalloidin (BBZ-Phalloidin) and confirmed by fluorescence microscopy. Measurements of the TER generated values up to 2020 Ω/cm2. A dose response of thrombin was observed, showing the permeability changes in the MDCK monolayer and subsequent recovery. A relationship between TER values and cytoskeletal organization was also observed and discussed.
Faheem Shah,Naeemullah,Tasneem Gul Kazi,Rafaqat Ali Khan,Murtaza Sayed,Hassan Imran Afridi,Khizar Hussain Shah,Jan Nisar 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.48 No.-
A novel restricted access sorbents based micro solid phase extraction (RAS-mSPE) for the extraction ofheavy metal from biological samples has been developed. Cadmium (Cd) and manganese (Mn) weredirectly extracted skipping the tedious, time consuming and expensive sample preparation stepexcluding all of the existing proteins. Sorbent’s (activated carbon cloths) surface was modified withbovine serum albumin through glutaraldehyde to make restricted access sorbent (RAS). Differentvariables affecting the extraction efficiency were selected for optimization. The limits of detectionobtained for Cd and Mn were 0.252 and 0.214 mg L 1, respectively. Analyte recoveries in fortified humanwhole blood serum and milk samples were found in the range of 90.3–103.9%. The procedure waseffectively used for Cd and Mn extraction in real samples devoid of any pretreatment step.
Electrophoretic deposition of PVA coated hydroxyapatite on 316L stainless steel
Nida Iqbal,Rabia Nazir,Anila Asif,Aqif Anwar Chaudhry,Muhammad Akram,Goh Yi Fan,Aftab Akram,Rashid Amin,박성하,Rafaqat Hussain 한국물리학회 2012 Current Applied Physics Vol.12 No.3
Polyvinyl alcohol (PVA) coated hydroxyapatite was deposited onto a 316L stainless steel substrate by electrophoretic deposition. Deposition was carried out in a methanol suspension at pH 5.5 using a graphite rod as an anode. Parameters such as PVA concentration, deposition voltage and time were optimized to achieve a homogeneous, crack-free adhesive coating. Techniques such as X-ray diffraction and Fourier transform infrared spectroscopy were used to study the phase composition of the coated materials and the stability of hydroxyapatite in the presence of PVA.