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Catalina Quintero-Quiroz,Natalia Acevedo,Jenniffer Zapata-Giraldo,Luz E. Botero,Julián Quintero,Diana Zárate-Triviño,Jorge Saldarriaga,Vera Z. Pérez 한국생체재료학회 2020 생체재료학회지 Vol.24 No.1
Background: Chemical reduction has become an accessible and useful alternative to obtain silver nanoparticles (AgNPs). However, its toxicity capacity depends on multiple variables that generate differences in the ability to inhibit the growth of microorganisms. Thus, optimazing parameters for the synthesis of AgNPs can increase its antimicrobial capacity by improving its physical-chemical properties. Methods: In this study a Face Centered Central Composite Design (FCCCD) was carried out with four parameters: AgNO3 concentration, sodium citrate (TSC) concentration, NaBH4 concentration and the pH of the reaction with the objective of inhibit the growth of microorganisms. The response variables were the average size of AgNPs, the peak with the greatest intensity in the size distribution, the polydispersity of the nanoparticle size and the yield of the process. AgNPs obtained from the optimization were characterized physically and chemically. The antimicrobial activity of optimized AgNPs was evaluated against Staphylococcus aureus, Escherichia coli, Escherichia coli AmpC resistant, and Candida albicans and compared with AgNPs before optimization. In addition, the cytotoxicity of the optimized AgNPs was evaluated by the colorimetric assay MTT (3- (4,5- Dimethylthiazol- 2- yl)- 2, 5 - Diphenyltetrazolium Bromide). Results: It was found that the four factors studied were significant for the response variables, and a significant model (p <0.05) was obtained for each variable. The optimal conditions were 8 for pH and 0.01 M, 0.0 6M, 0.01 M for the concentration of TSC, AgNO3, and NaBH4, respectively. Optimized AgNPs spherical and hemispherical were obtained, and 67.66% of it had a diameter less than 10.30 nm. A minimum bactericidal concentration (MBC) and minimum fungicidal Concentration (MFC) of optimized AgNPs was found against Staphylococcus aureus, Escherichia coli, Escherichia coli AmpC resistant, and Candida albicans at 19.89, 9.94, 9.94, 2.08 μg/mL, respectively. Furthermore, the lethal concentration 50 (LC50) of optimized AgNPs was found on 19.11 μg/mL and 19.60 μg/mL to Vero and NiH3T3 cells, respectively. Conclusions: It was found that the factors studied were significant for the variable responses and the optimization process used was effective to improve the antimicrobial activity of the AgNPs.
Quintero, Jesus,Juamperez, Javier,Gonzales, Emmanuel,Julio, Ecaterina,Mercadal-Hally, Maria,Collado-Hilly, Mauricette,Marin-Sanchez, Ana,Charco, Ramon The Korean Society of Pediatric Gastroenterology 2020 Pediatric gastroenterology, hepatology & nutrition Vol.23 No.2
We present an 8 years old girl who was diagnosed at 6 months of age of Progressive Familial Intrahepatic Cholestasis type 2. Although liver transplantation (LT) was classically considered curative for these patients, cholestasis recurrence with normal gamma-glutamyl transpeptidase (GGT), mediated by anti-bile salt export pump (BSEP) antibodies after LT (auto-antibody Induced BSEP Deficiency, AIBD) has been recently reported. Our patient underwent LT at 14 months. During her evolution, patient presented three episodes of acute rejection. Seven years after the LT, the patient presented pruritus with cholestasis and elevation of liver enzymes with persistent normal GGT. Liver biopsy showed intrahepatic cholestasis and giant-cell transformation with very low BSEP activity. Auto-antibodies against BSEP were detected therefore an AIBD was diagnosed. She was treated with Rituximab and immunoadsorption with resolution of the AIBD. As a complication of the treatment she developed a pneumocystis infection successfully treated with corticoids, cotrimoxazol and anidulafungin.
Quintero, Russ,Wei, Jun,Galati, Nestore,Nanni, Antonio Korea Concrete Institute 2007 International Journal of Concrete Structures and M Vol.1 No.1
The purpose of this paper is the assessment of the capacity of the reinforced concrete (RC) elements of an arch bridge when they are subjected to contact and near-contact explosive charges of various amounts, and the estimation of the critical charges for these components. The bridge considered is the Tenza Viaduct, a decommissioned structure south of Naples, Italy. Its primary elements, deck, piers and arches were analyzed. The evaluation was accomplished via numerical analyses that made possible to obtain the elements dynamic response when they are exposed to blast loading conditions. To evaluate the member's capacities, failure criteria for deck, piers and arches were proposed based on concrete damage parameters. Additionally, curves relating the explosive charge to the residual capacity and to damage level of the elements were also developed. The results of this work were taken into account to investigate the progressive collapse of the global structure.
Quintero Noda, C.,Villanueva, G. L.,Katsukawa, Y.,Solanki, S. K.,Orozco Suá,rez, D.,Ruiz Cobo, B.,Shimizu, T.,Oba, T.,Kubo, M.,Anan, T.,Ichimoto, K.,Suematsu, Y. Springer-Verlag 2018 Astronomy and astrophysics Vol.610 No.-
<P>Of the two solar lines, K I<I>D</I>1 and <I>D</I>2, almost all attention so far has been devoted to the <I>D</I>1 line, as <I>D</I>2 is severely affected by an O2 atmospheric band. This, however, makes the latter appealing for balloon and space observations from above (most of) the Earth’s atmosphere. We estimate the residual effect of the O2 band on the K I<I>D</I>2 line at altitudes typical for stratospheric balloons. Our aim is to study the feasibility of observing the 770 nm window. Specifically, this paper serves as a preparation for the third flight of the Sunrise balloon-borne observatory. The results indicate that the absorption by O2 is still present, albeit much weaker, at the expected balloon altitude. We applied the obtained O2 transmittance to K I<I>D</I>2 synthetic polarimetric spectra and found that in the absence of line-of-sight motions, the residual O2 has a negligible effect on the K I<I>D</I>2 line. On the other hand, for Doppler-shifted K I<I>D</I>2 data, the residual O2 might alter the shape of the Stokes profiles. However, the residual O2 absorption is sufficiently weak at stratospheric levels that it can be divided out if appropriate measurements are made, something that is impossible at ground level. Therefore, for the first time with Sunrise III, we will be able to perform polarimetric observations of the K I<I>D</I>2 line and, consequently, we will have improved access to the thermodynamics and magnetic properties of the upper photosphere from observations of the K I lines.</P>
Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices
Quintero Sierra Lindsey Alejandra,Busato Alice,Zingaretti Nicola,Conti Anita,Biswas Reetuparna,Governa Maurizio,Vigato Enrico,Parodi Pier Camillo,Bernardi Paolo,Sbarbati Andrea,Conti Giamaica 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3
BACKGROUND: Breast reconstruction after mastectomy using silicone implants is a surgical procedure that occasionally leads to capsular contracture formation. This phenomenon constitutes an important and persistent cause of morbidity, and no successful therapies are available to date. Recently, the use of acellular membranes as a protective material for silicone prostheses has been gaining attention due to their ability to prevent this adverse outcome. For this reason, the evaluation of the tissue-material integration and the induced biostimulation by acellular membranes results crucial. Evaluation of in vivo tissue integration and biostimulation induced by three different natural acellular collagen membranes. METHODS: Scanning electron microscopy was performed to analyse the membrane porosity and cells-biomaterial interaction in vitro, both in dry and wet conditions. Adipose-derived stem cells were cultured in the presence of membranes, and the colonisation capacity and differentiation potential of cells were assessed. In vivo tests and ex vivo analyses have been performed to evaluate dermal integration, absorption degree and biostimulation induced by the evaluated membrane. RESULTS: Analysis performed in vitro on the three different acellular dermal matrices evidenced that porosity and the morphological structure of membranes influence the liquid swelling ratio, affecting the cell mobility and the colonisation capacity. Moreover, the evaluated membranes influenced in different manner the adipose derived stem cells differentiation and their survival. In vivo investigation indicated that the absorption degree and the fluid accumulation surrounding the implant were membrane-dependent. Finally, ex vivo analysis confirmed the membrane-dependent behavior revealing different degree of tissue integration and biostimulation, such as adipogenic stimulation. CONCLUSION: The physico-chemical characteristics of the membranes play a key role in the biostimulation of the cellular environment inducing the development of well-organized adipose tissue.
Catalina Quintero-Quiroz,Luz E. Botero,Diana Zárate-Triviño,Natalia Acevedo-Yepes,Jorge Saldarriaga Escobar,Vera Z. Pérez,Luis Javier Cruz Riano 한국생체재료학회 2020 생체재료학회지 Vol.24 No.3
Background: The presence of skin problems in patients using external lower limb prosthesis is recurrent. This has generated the need to develop interfaces for prosthesis with the ability to control microbial growth. Silver nanoparticles (AgNPs) have been implemented in the development of biomaterials because of their high antimicrobial activity. This article discusses the development of an AgNP-containing polymer composite with antimicrobial activity for developing prosthetic liners. Methods: AgNPs were synthesized using a photochemical method and certain physicochemical properties were characterized. Furthermore, the antimicrobial activity of AgNPs against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus (MRSA), was assessed on the basis of their minimum inhibitory concentrations (MICs). AgNPs were incorporated into a silicon elastomer to assess certain physicomechanical properties, antimicrobial activity and cytotoxic effect of the material. Results: Themaximum antimicrobial activity of the material against Staphylococcus aureus ATCC 25923 andMRSA was 41.58% ±2.97% at AgNP concentration of 32.98 μg/mL and 14.85% ±5.94% at AgNP concentration of 16.49 μg/mL, respectively. Additionally, the material exhibited tensile yield strength, rupture tensile strength, and tensile modulus of elasticity of 0.70 - 1.10 MPa, 0.71–1.06 MPa, and 0.20 - 0.30 MPa, respectively. The mechanical characteristics of the material were within the acceptable range for use in external lower limb prosthetic and orthotic interfaces. Conclusions: It was possible to incorporate the AgNPs in a silicone elastomer, finding that the composite developed presented antimicrobial activity against Staphylococcus aureus ATCC 25923 and MRSA when compared to non-AgNP material samples.
Jesús Quintero,Javier Juamperez,Emmanuel Gonzales,Ecaterina Julio,Maria Mercadal-Hally,Mauricette Collado-Hilly,Ana Marín-Sánchez,Ramon Charco 대한소아소화기영양학회 2020 Pediatric gastroenterology, hepatology & nutrition Vol.23 No.2
We present an 8 years old girl who was diagnosed at 6 months of age of Progressive Familial Intrahepatic Cholestasis type 2. Although liver transplantation (LT) was classically considered curative for these patients, cholestasis recurrence with normal gamma-glutamyl transpeptidase (GGT), mediated by anti-bile salt export pump (BSEP) antibodies after LT (auto-antibody Induced BSEP Deficiency, AIBD) has been recently reported. Our patient underwent LT at 14 months. During her evolution, patient presented three episodes of acute rejection. Seven years after the LT, the patient presented pruritus with cholestasis and elevation of liver enzymes with persistent normal GGT. Liver biopsy showed intrahepatic cholestasis and giant-cell transformation with very low BSEP activity. Auto-antibodies against BSEP were detected therefore an AIBD was diagnosed. She was treated with Rituximab and immunoadsorption with resolution of the AIBD. As a complication of the treatment she developed a pneumocystis infection successfully treated with corticoids, cotrimoxazol and anidulafungin.
Luis Quintero-Fong,Dina Orozco-Dávila 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
In the mass rearing of fruit flies, the adult emergence time is gradual, due to factors such as variation in food consumption in the larval phase, the female oviposition behavior and environmental conditions, among others. Here, we investigate the effect of emergence time on sex ratio, fecundity, fertility, body size and mating performance in mass-reared Anastrepha ludens (Diptera: Tephritidae) flies. The results of the study indicated that the emergence of the flies is gradual and lasts approximately 96 h, but the highest proportion of emerged flies was observed at 48 h. The flies emerging at 24 and 48 h showed higher fecundity than those emerging at 72 and 96 h, but there were no significant differences in the sex ratio, fertility and male mating performance. Body size was significant, the flies that emerged at 24, 48 and 72 h were bigger than those emerged at 96 h. The discussion focuses on the effect of emergence time on the rearing processes and release of the Mexican fruit fly.