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      • Effect of Media Composition on Mass Cultur of Plant Growth Promoting Methlyobacterium oryzae

        Insso Hong, Hari P. Deka Boruah, Puneet S. Chauhan, Minkyoung Lee, Sungman Woo, Tongmin Sa 忠北大學校 農業科學硏究所 2010 農業科學硏究 Vol.26 No.1

        Mass culture of plant growth promoting Methvlobacterium oryzae CBMB20 for its utilization as biofertilizer for different crops has been carried out. This study was conducted to examine the effect of media composition on mass culture of M oryzae CBMB20. From different carbon sources used, the result demonstrated that M oryzae CBMB20 had better growth in sodium succinate than in methanol. The effect of 1 and 2% inoculum size on the growth rate of M oryzea CBMB20 has been checked for mass culture. Results have shown higher biomass production when 2% inoculum was used to the medium. M oryzae CBMB20 produced higher indole-3-acetic acid(IAA) in minimal mineral salt (MMS) media and have higher 1-aminocyclopropane-1-carboxylate deaminate (ACCd) activity in yeast and malt extract peptone (YMEP) media. The comparison of MMS and YMEP media for the growth of M oryzae CBMC20 was carried out and results showed better growth in YMEP medium. These results demon-started that sodium succinate is the best carbon source for M oryzae CBMB20. Moreover, M oryzae CBMB20 is best grown in YMEP medium for mass culture (since M oryzae CBMB20 has been reported as plant growth promoting bacteria for many crops such as red pepper, rice, tomato etc.). These results may be useful in the further exploration of M oryzae CBMC20 under field conditions.

      • KCI등재

        Effects of Metal and Metalloid Contamination on Microbial Diversity and Activity in Agricultural Soils

        Tipayno, Sherlyn C.,Chauhan, Puneet S.,Woo, Sung-Man,Hong, Bo-Hee,Park, Kee-Woong,Chung, Jong-Bae,Sa, Tong-Min Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1

        The continuous increase in the production of metals and their subsequent release into the environment has lead to increased concentration of these elements in agricultural soils. Because microbes are involved in almost every chemical transformations taking place in the soil, considerable attention has been given to assessing their responses to metal contaminants. Short-term and long-term exposures to toxic metals have been shown to reduce microbial diversity, biomass and activities in the soil. Several studies show that microbial parameters like basal respiration, metabolic quotient, and enzymatic activities, including those of oxidoreductases and those involved in the cycle of C, N, P and other elements, exhibit sensitivity to soil metal concentrations. These have been therefore, regarded as good indices for assessing the impact of metal contaminants to the soil. Metal contamination has also been extensively shown to decrease species diversity and cause shifts in microbial community structure. Biochemical and molecular techniques that are currently being employed to detect these changes are continuously challenged by several limiting factors, although showing some degree of sensitivity and efficiency. Variations and inconsistencies in the responses of bioindicators to metal stress in the soil can also be explained by differences in bioavailability of the metal to the microorganisms. This, in turn, is influenced by soil characteristics such as CEC, pH, soil particles and other factors. Therefore, aside from selecting the appropriate techniques to better understand microbial responses to metals, it is also important to understand the prevalent environmental conditions that interplay to bring about observed changes in any given soil parameter.

      • KCI등재

        Exploring the Potential of Bacteria-Assisted Phytoremediation of Arsenic-Contaminated Soils

        Shagol, Charlotte C.,Chauhan, Puneet S.,Kim, Ki-Yoon,Lee, Sun-Mi,Chung, Jong-Bae,Park, Kee-Woong,Sa, Tong-Min Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1

        Arsenic pollution is a serious global concern which affects all life forms. Being a toxic metalloid, the continued search for appropriate technologies for its remediation is needed. Phytoremediation, the use of green plants, is not only a low cost but also an environmentally friendly approach for metal uptake and stabilization. However, its application is limited by slow plant growth which is further aggravated by the phytotoxic effect of the pollutant. Attempts to address these constraints were done by exploiting plant-microbe interactions which offers more advantages for phytoremediation. Several bacterial mechanisms that can increase the efficiency of phytoremediation of As are nitrogen fixation, phosphate solubilization, siderophore production, ACC deaminase activity and growth regulator production. Many have been reported for other metals, but few for arsenic. This mini-review attempts to present what has been done so far in exploring plants and their rhizosphere microbiota and some genetic manipulations to increase the efficiency of arsenic soil phytoremediation.

      • KCI등재

        Comparison of Plant Growth Promoting Methylobacterium spp. and Exogenous Indole-3-Acetic Acid Application on Red Pepper and Tomato Seedling Development

        Hari P. Deka Boruah,Puneet S. Chauhan,Woo-Jong Yim(임우종),Gwang-Hyun Han(한광현),Tong-Min Sa(사동민) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.1

        무균 및 온실조건에서 indole-3-acetic acid (IAA)의 처리와 1-aminocyclopropane-1-carboxylate deaminase (ACCD) 및 IAA 활성을 갖는 Methylobacterium 균주 접종 시 토마토와 고추의 생장을 비교 평가하였다. 무균조건에서 1.0 ㎍ mL?¹의 IAA는 고추와 토마토의 뿌리생장을 촉진시키는데 비해 10.0 ㎍ mL?¹ 이상의 높은 농도에서는 뿌리생장이 억제되었다. 그러나 높은 ACCD 활성을 갖고, IAA 활성은 낮거나 가지고 있지 않은 Methylobacterium 균주들을 접종하였을 때에는 고추와 토마토 모두 IAA 처리구 보다 뿌리생장이 증진되는 것을 확인하였다. 마찬가지로 온실조건에서 Methylobacterium 균주들을 접종했을 때, 마디길이와 잎의 크기 그리고 단위 면적당 잎의 무게(SLW)에서 유의성 있는 증진효과를 보였다. 전반적인 식물 생장에서 저농도의 IAA 처리 효과는 Methylobacterium의 효과와 비슷한 경향을 나타냈다. 유묘의 지상부 길이는 ACCD 활성과 IAA 생산능을 갖는 Methylobacterium 균주 처리구에서 유의성 있는 증가를 확인할 수 있었으며, 전체 건물중 또한 Methylobacterium 처리 시 유의성 있는 증진 효과를 확인 할 수 있었다. 하지만 고농도의 IAA는 고추와 토마토의 생물량을 억제시켰다. 이러한 결과는 접종 균주의 IAA와 ACCD가 고추와 토마토 유묘의 생장 증진에 영향을 끼친다는 것을 증명한다. A comparative study was performed in gnotobiotic and greenhouse conditions to evaluate the effect of exogenous application of indole-3-acetic acid (IAA) and inoculation of Methylobacterium spp. possessing 1-aminocyclopropane-1-carboxylate deaminase (ACCD) and IAA activity on red pepper and tomato seedling growth and development. Application of 1.0 ㎍ mL?¹ IAA positively influenced root growth while high concentrations (>10.0 ㎍ mL?¹) suppressed root growth of red pepper and tomato under gnotobiotic condition. On the other hand, inoculation of Methylobacterium strains with ACCD activity and IAA or without IAA enhanced root growth in both plants. Similarly, under greenhouse condition the inoculation of Methylobacterium sp. with ACCD activity and IAA enhanced plant fitness recorded as average nodal length and specific leaf weight (SLW) but the effect is comparable with the application of low concentrations of IAA. Seedling length was significantly increased by Methylobacterium strains while total biomass was enhanced by Methylobacterium spp. and exogenous applications of < 10.0 ㎍ mL?¹ IAA. High concentrations of IAA retard biomass accumulation in red pepper and tomato. These results confirm that bacterial strains with plant growth promoting characters such as IAA and ACCD have characteristic effects on different aspects of growth of red pepper and tomato seedlings which is comparable or better than exogenous applications of synthetic IAA.

      • KCI등재

        Exploring the Potential of Bacteria-Assisted Phytoremediation of Arsenic-Contaminated Soils

        Charlotte C. Shagol,Puneet S. Chauhan,Kiyoon Kim,Sunmi Lee,Jongbae Chung,Keewoong Park,Tongmin Sa 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.1

        Arsenic pollution is a serious global concern which affects all life forms. Being a toxic metalloid, the continued search for appropriate technologies for its remediation is needed. Phytoremediation, the use of green plants, is not only a low cost but also an environmentally friendly approach for metal uptake and stabilization. However, its application is limited by slow plant growth which is further aggravated by the phytotoxic effect of the pollutant. Attempts to address these constraints were done by exploiting plant-microbe interactions which offers more advantages for phytoremediation. Several bacterial mechanisms that can increase the efficiency of phytoremediation of As are nitrogen fixation, phosphate solubilization, siderophore production, ACC deaminase activity and growth regulator production. Many have been reported for other metals, but few for arsenic. This mini-review attempts to present what has been done so far in exploring plants and their rhizosphere microbiota and some genetic manipulations to increase the efficiency of arsenic soil phytoremediation.

      • KCI등재

        Effects of Metal and Metalloid Contamination on Microbial Diversity and Activity in Agricultural Soils

        Sherlyn C. Tipayno,Puneet S. Chauhan,Sungman Woo,Bohee Hong,Keewoong Park,Jongbae Chung,Tongmin Sa 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.1

        The continuous increase in the production of metals and their subsequent release into the environment has lead to increased concentration of these elements in agricultural soils. Because microbes are involved in almost every chemical transformations taking place in the soil, considerable attention has been given to assessing their responses to metal contaminants. Short-term and long-term exposures to toxic metals have been shown to reduce microbial diversity, biomass and activities in the soil. Several studies show that microbial parameters like basal respiration, metabolic quotient, and enzymatic activities, including those of oxidoreductases and those involved in the cycle of C, N, P and other elements, exhibit sensitivity to soil metal concentrations. These have been therefore, regarded as good indices for assessing the impact of metal contaminants to the soil. Metal contamination has also been extensively shown to decrease species diversity and cause shifts in microbial community structure. Biochemical and molecular techniques that are currently being employed to detect these changes are continuously challenged by several limiting factors, although showing some degree of sensitivity and efficiency. Variations and inconsistencies in the responses of bioindicators to metal stress in the soil can also be explained by differences in bioavailability of the metal to the microorganisms. This, in turn, is influenced by soil characteristics such as CEC, pH, soil particles and other factors. Therefore, aside from selecting the appropriate techniques to better understand microbial responses to metals, it is also important to understand the prevalent environmental conditions that interplay to bring about observed changes in any given soil parameter.

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