Growth Inhibitory Nature of Artemisia annua Extract against Culex quinquefasciatus (Say)

Preeti Sharma,Lalit Mohan,C.N. Srivastava 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.4

Petroleum ether (Pee), carbon tetrachloride (Cte) and methanol extract (Mee) of Artemisia annua, Chenopodium album and Sonchus oleraceus were screened for their efficacy against Culex quinquefasciatus larvae. Pee of A. annua, Mee of A. annua and Ch. album, Cte of A. Annua were found effective in descending order after 24 and 48 hrs of treatment. Pee of A. annua, the most potent extract with LC50 78.2 ppm was selected to study its influence on the development and metamorphosis of the culicine mosquito. The extract significantly affected the hatching, larval development, pupal transformation and also lengthened the larval and pupal periods. Growth index was remarkably reduced. Treated culicine eggs, larvae and pupae showed deformities including disruption of the body wall, distorted alimentary canal, damaged tracheal network and arrested histogenesis. The extract has remarkable effect on the metamorphosis and high larvicidal potential, hence, can be used as an effective alternative to the existing synthetic pesticides for the control of Cx. quinquefasciatus.

Role of Photosensitization of Certain Phytotoxins in the Management of Culex quinqueasciatus

Preeti Sharma Rawat,Lalit Mohan,C. N. Srivastava 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

Impact Analysis of Neem Kernel Extracts on the Developmental Profile of Anopheles stephensi

Sharma, Preeti,Mohan, Lalit,Srivastava, C.N. Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.1

Laboratory evaluation of the larvicidal efficacy of Ageratum conyzoides, Argemone mexicana and Azadirachta indica was carned out against Anopheles stephensi to develop an effective ecofriendly insecticide of plant origin. The methanol extract of A. indica was observed remarkably active with $LC_{50}$ values 18.2 and 13.1 ppm after 24 and 48 hours of exposure. Petroleum ether extracts of Ag. conyzoides with $LC_{50}$ 425.6 and 267.9 ppm, carbon tetra chloride extract of Ar. mexicana with $LC_{50}$ 313.2 and 240.3 ppm and petroleum ether extract of Az. indica with $LC_{50}$ 189.1 and 53. 5 ppm after 24 and 48 hours of exposure were also found effective. The impact of Az. indica methanol extract on the life cycle of malaria vector was further observed. Methanol extract of Az. indica causes deformities in different developmental stages of the malaria vector. Total developmental period was also extended from 10 to 15 It remarkably influenced hatching rates, larval-pupal transformation and development, adult emergence and growth index. Larval and pupal periods were also significantly prolonged.

Impact analysis of neem kernel extracts on the developmental profile of Anopheles stephensi

Preeti Sharma,Lalit Mohan,C.N. Srivastava 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.1

evaluation of the larvicidal effi-cacy ofAgeratum conyzoides,Argemone mexicanaand Azadirachta indicawas carried out againstAno-pheles stephensito develop an effective ecofriendlyinsecticide of plant origin. The methanol extract ofA. indicawas observed remarkably active with LC50values 18.2 and 13.1 ppm after 24 and 48 hours ofexposure. Petroleum ether extracts ofAg. conyzoideswith LC50 425.6 and 267.9 pm, carbon tetra chlo-ride extract ofAr. mexicanawith LC50 313.2 and240.3 ppm and petroleum ether extract ofAz. indicawith LC50 189.1 and 53. 5 ppm after 24 and 48hours of exposure were also found effective. Theimpact of Az.indica methanol extract on the lifecycle of malaria vector was further observed. Me-thanol extract of Az.indica causes deformities indifferent developmental stages of the malaria vector.Total developmental period was also extended from10 to 15 It remarkably influenced hatching rates,larval-pupal transformation and development, adultemergence and growth index. Larval and pupalKey words An. stephensi, Ageratum, Argemone,Azadirachta, phytoextract, metamorphosis, larvicide.

Actinobacteria from Cow Feces: Isolation, Identification and Screening for Industrially Important Secondary Metabolites

( Preeti Semwal ),( Vinay Rawat ),( Pushpendra Sharma ),( Mamta Baunthiyal ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 한국미생물·생명공학회지 Vol.46 No.1

Actinobacterial strains isolated from Cow feces were studied for their antifungal attributes against phytopathogens and industrially important enzymes. A total of 30 Actinobacterial strains were obtained from 10 samples of cow feces. All the strains were belonging to the genera Streptomyces on the basis of morphological and chemotaxonomic analysis. During preliminary screening, out of 30 strains, 15 strains (50%) showed antifungal activity against five fungal phytopathogens including Aspergillus niger, Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina and Rhizoctonia solani. While, isolate GBTCF-26 was found to be most active against R. solani with 62.2% inhibition of fungal mycelium, GBTCF-09 was prominent against F. solani and F. oxysporum with percent inhibition of 61.1% and 58.8%, respectively. Out of 30 strains, 19 (63.3%), 16 (53.3%), 11 (36.7%), 10 (33.3%), 4 (13.3%) and 8 (26.7%) strains were producing amylase, caseinase, gelatinase, lipase, chitinase and cellulose, respectively. The selected strains, GBTCF-09, GBTCF- 21 and GBTCF-26, were identified as Streptomyces sp. on the basis of their 16S rDNA sequence. The study supports the idea that the Actinobacteria from unique niches (Cow feces) possess the production potential of industrially important enzymes including bioactive molecules.

Three Weekly Versus Weekly Cisplatin as Radiosensitizer in Head and Neck Cancer: a Decision Dilemma

Negi, Preety,Kingsley, Pamela Alice,Srivastava, Himanshu,Sharma, Surender Kumar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4

Cisplatin-based concurrent chemoradiation plays an undisputed key role as definitive treatment in unresectable patients with locally advanced squamous cell carcinoma head and neck or as an organ preservation strategy. Treatment with 100 mg/m2 3-weekly cisplatin is considered the standard of care but is often associated with several adverse events. The optimum drug schedule of administration remains to be defined and presently, there is insufficient data limiting conclusions about the relative tolerability of one regimen over the other. This review addresses regarding the optimal dose schedule of cisplatin focusing mainly on three-weekly and weekly dose of cisplatin based concurrent chemoradiotherapy in locally advanced head and neck cancer with an emphasis on mucositis, dermatitis, systemic toxicity, compliance, and treatment interruptions. To derive a definitive conclusion, large prospective randomized trials are needed directly comparing standard 3-weekly cisplatin ($100mg/m^2$) with weekly schedule ($30-40mg/m^2$) of concurrent cisplatin based chemoradiotherapy in locally advanced squamous cell carcinoma head and neck.

Syndecan-1 (sCD138) levels in chronic lymphocytic leukemia: clinical and hematological correlations

Monica Sharma,Seema Tyagi,Preeti Tripathi,Tulika Seth 대한혈액학회 2018 Blood Research Vol.53 No.3

Background Syndecan-1 (sCD138) has recently been suggested to predict the clinical course of ear-ly-stage chronic lymphocytic leukemia (CLL), but few studies have been reported. This study assessed the role of syndecan-1 in the prognosis of patients with CLL and its correla-tion with other prognostic markers. Methods This prospective study was performed in the hematology department of an Indian tertiary care center, over nineteen months (Jun. 2009‒Jan. 2011). Forty-nine new patients with CLL presented during this period and were included. Twenty age- and gender-matched healthy patients served as controls, and six patients with multiple myeloma were included as positive controls. Baseline serum syndecan-1 concentrations were measured for all patients at presentation using ELISA (Diaclone, Besancon, France). At baseline, patients were divided into low (N=10), intermediate (N=18) and high (N=21) risk cohorts. Serum syndecan-1 levels in these patient subgroups were compared with clinical and laboratory parameters. Results The median syndecan-1 level in patients with CLL (73.32 ng/mL, range, 28.71‒268.0 ng/mL) was marginally higher than that in healthy patients (63.10 ng/mL, range, 55.0‒75.11 ng/mL). At presentation, syndecan-1 levels in patients with CLL correlated strongly with symptomatic disease (cytopenias, P=0.004) and higher clinical stage (Rai stage III and IV, P=0.001) markers and poorly with 2-microglobulin level (P=0.270), diffuse BM infiltration (P=0.882), and surrogate mutation status markers (CD 38, P=0.174 and ZAP-70, P=0.459). Syndecan-1 levels dichotomized by the median value were higher with progressive disease markers, e.g. shorter lymphocyte doubling time (LDT, P=0.015) and increased treatment (P=0.099). ConclusionIn CLL, serum syndecan-1 (sCD138) levels at presentation correlate with disease burden, and higher baseline levels may predict early treatment.

Syndecan-1 (sCD138) levels in chronic lymphocytic leukemia: clinical and hematological correlations

Monica Sharma,Seema Tyagi,Preeti Tripathi,Tulika Seth 대한혈액학회 2018 Blood Research Vol.53 No.3

Background Syndecan-1 (sCD138) has recently been suggested to predict the clinical course of ear-ly-stage chronic lymphocytic leukemia (CLL), but few studies have been reported. This study assessed the role of syndecan-1 in the prognosis of patients with CLL and its correla-tion with other prognostic markers. Methods This prospective study was performed in the hematology department of an Indian tertiary care center, over nineteen months (Jun. 2009‒Jan. 2011). Forty-nine new patients with CLL presented during this period and were included. Twenty age- and gender-matched healthy patients served as controls, and six patients with multiple myeloma were included as positive controls. Baseline serum syndecan-1 concentrations were measured for all patients at presentation using ELISA (Diaclone, Besancon, France). At baseline, patients were divided into low (N=10), intermediate (N=18) and high (N=21) risk cohorts. Serum syndecan-1 levels in these patient subgroups were compared with clinical and laboratory parameters. Results The median syndecan-1 level in patients with CLL (73.32 ng/mL, range, 28.71‒268.0 ng/mL) was marginally higher than that in healthy patients (63.10 ng/mL, range, 55.0‒75.11 ng/mL). At presentation, syndecan-1 levels in patients with CLL correlated strongly with symptomatic disease (cytopenias, P=0.004) and higher clinical stage (Rai stage III and IV, P=0.001) markers and poorly with 2-microglobulin level (P=0.270), diffuse BM infiltration (P=0.882), and surrogate mutation status markers (CD 38, P=0.174 and ZAP-70, P=0.459). Syndecan-1 levels dichotomized by the median value were higher with progressive disease markers, e.g. shorter lymphocyte doubling time (LDT, P=0.015) and increased treatment (P=0.099). ConclusionIn CLL, serum syndecan-1 (sCD138) levels at presentation correlate with disease burden, and higher baseline levels may predict early treatment.

Actinobacteria from Cow Feces: Isolation, Identification and Screening for Industrially Important Secondary Metabolites

Semwal, Preeti,Rawat, Vinay,Sharma, Pushpendra,Baunthiyal, Mamta The Korean Society for Microbiology and Biotechnol 2018 한국미생물·생명공학회지 Vol.46 No.1

Actinobacterial strains isolated from Cow feces were studied for their antifungal attributes against phytopathogens and industrially important enzymes. A total of 30 Actinobacterial strains were obtained from 10 samples of cow feces. All the strains were belonging to the genera Streptomyces on the basis of morphological and chemotaxonomic analysis. During preliminary screening, out of 30 strains, 15 strains (50%) showed antifungal activity against five fungal phytopathogens including Aspergillus niger, Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina and Rhizoctonia solani. While, isolate GBTCF-26 was found to be most active against R. solani with 62.2% inhibition of fungal mycelium, GBTCF-09 was prominent against F. solani and F. oxysporum with percent inhibition of 61.1% and 58.8%, respectively. Out of 30 strains, 19 (63.3%), 16 (53.3%), 11 (36.7%), 10 (33.3%), 4 (13.3%) and 8 (26.7%) strains were producing amylase, caseinase, gelatinase, lipase, chitinase and cellulose, respectively. The selected strains, GBTCF-09, GBTCF-21 and GBTCF-26, were identified as Streptomyces sp. on the basis of their 16S rDNA sequence. The study supports the idea that the Actinobacteria from unique niches (Cow feces) possess the production potential of industrially important enzymes including bioactive molecules.

Larvicidal activity of Pseudocalymma alliaceum and Allium sativum against Culex quinquefasciatus (Say)

Shrankhla,Preeti Sharma,Lalit Mohan,Chand Narayan SRIVASTAVA 한국곤충학회 2011 Entomological Research Vol.41 No.6

The larvicidal activity of the plant extracts Pseudocalymma alliaceum and Allium sativum were determined against Culex quinquefasciatus. The hexane extract of P. alliaceum and the petroleum ether extract of A. sativum exhibited larvicidal efficacy against Cx. quinquefasciatus larvae. Extracts of P. alliaceum resulted in concentrations that produced 50% mortality LC_50 and LC_90 values of 2.49 and 15.06 ppm, respectively, after 24 h and 1.16 and 8.45 ppm after 48 h. Extracts of A. sativum resulted in LC_50 and LC_90 values of 8.38 and 29.15 ppm after 24 h and 7.28 and 44.19 ppm after 48 h of exposure, respectively. The results indicate that the plant extract component(s) present in the hexane extract of P. alliaceum leaves demonstrated greater potential as an efficient larvicide than A. sativum against Cx. quinquefasciatus.