Molecular organization of the B mating type locus of a Lentinula edodes monokaryon strain SUP2

Bao Da-Peng Chen Ming-Jie,Song Wen-Hua,Song Chun-yan,Zhang Mei-Yan,Chen Xiang,Lin Nan 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-

Molecular organization of the B mating type locus of a Lentinula edodes monokaryon strain SUP2

Bao Da-Peng Chen Ming-Jie,Song Wen-Hua,Song Chun-yan,Zhang Mei-Yan,Chen Xiang,Lin Nan 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Lentinula edodes is an important cultivated mushroom in China. The development of Lentinula edodes production promotes more studies on it. In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the whole genome sequencing of the protoplast monokaryon of Lentinula edodes strain 135, to amplify STE3-like pheromone receptor gene and its flanking conserved genes in the protoplast monokaryon strain SUP2 derived from Lentinula edodes strain Suxiang and 33655bp DNA sequence was obtained. By BlastX search, seven putative genes were identified, and three of them are pheromone receptor encoded genes. Furthermore, near to two pheromone receptor genes, four genes encoding proteins with conserved motifs of pheromone precursors were found. This study firstly reveals the molecular organization of the B mating type locus of Lentinula edodes.

H-형태 양친매성 펜타블록 공중합체의 화학효소적 합성과 자기회합거동 평가

Peng Chen,Ya Peng Li,Can Jin Li,Xin Lei Meng,Bao Zhang,Ming Zhu,Yan-jing Liu,Jing Yuan Wang 한국고분자학회 2013 폴리머 Vol.37 No.3

H-shaped amphiphilic pentablock copolymers (PSt)2-b-PCL-b-PEO-b-PCL-b-(PSt)2 was synthesized via chemoenzymatic method by combining enzyme-catalyzed ring-opening polymerization (eROP) of ε-caprolactone (ε-CL) and atom transfer radical polymerization (ATRP) of styrene. By this process, we obtained copolymers with controlled molecular weight and low polydispersity. The structure and composition of the obtained copolymers were characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC) and infrared spectroscopy analysis (IR). The crystallization behavior of the copolymers was analyzed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). The crystallization behavior of the H-shaped block copolymers demonstrated a PCL dominate crystallization. The self-assembly behavior of the copolymers was investigated in aqueous media. The hydrodynamic diameters of the copolymer micelles in aqueous solution were measured by dynamic light scattering (DLS). The morphology of the copolymer micelles was observed by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hydrodynamic diameters of spherical micelles declined gradually with the increase of the hydrophobic chain lengths of the copolymers. The critical micelle concentration (CMC) values were determined from fluorescence emission, and it was found that the CMCs decreased with an increase of PSt hydrophobic block lengths.

Novel DNAH1 Mutation Loci Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Literature Review

Zhuang Bao-Jun,Xu Su-Yun,Dong Liang,Zhang Pei-Hai,Zhuang Bao-Lin,Huang Xiao-Peng,Li Guang-Sen,You Yao-Dong,Chen Di'Ang,Yu Xu-Jun,Chang De-Gui 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.4

The protein encoded by dynein axonemal heavy chain 1 (DNAH1) is a part of dynein, which regulates the function of cilia and sperm flagella. The mutant of DNAH1 causes the deletion of inner dynein arm 3 in the flagellum, leading to multiple morphological abnormalities of the sperm flagella (MMAF) and severe asthenozoospermia. However, instead of asthenozoospermia and MMAF, the result caused by the mutation of DNAH1 remains unknown. Here we report a male infertility patient with severe asthenozoospermia and teratozoospermia. We found two heterozygous mutations in DNAH1 (c.6912C>A and c.7076G>T) and which were reported to be associated with MMAF for the first time. We next collected and analyzed 65 cases of DNAH1 mutation and found that the proportion of short flagella is the largest, while the bent flagella account for the smallest, and the incidence of head deformity is not high in the sperm of these patients. Finally, we also analyzed 31 DNAH1 mutation patients who were treated with intracytoplasmic sperm injection (ICSI) and achieved beneficial outcomes. We hope our research will be helpful in the diagnosis and treatment of male infertility caused by DNAH1 mutation.

중국의 해외직구사이트특성이 구매의도에 미치는 영향

포붕(Peng Bao),이종호(Jong-Ho Lee) 국제e-비즈니스학회 2018 e-비즈니스 연구 Vol.19 No.3

Research on Three Dimensional Reconstruction of Plant Root Based on spatial Geometry Structure and Morphology Architecture Parameters

Wu Peng,Wang Bao-kun,Leng Xin,Xie Peng-cheng 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.6

The root plays an important role in the ecosystem of plants. In order to solve the problem that to measure the root is difficult, this article proposes the three dimensional morphology model of plant root based on spatial geometry structure and morphology architecture parameters, and realize the three dimensional visualization of root. First, constructing three dimensional display model including root axis growing model, branch geometry model and root axis curve model; then combining topological structure of root, we confirm corresponding pixel, using morphology characteristic parameters outputted by root morphology to conduct the three dimensional reconstruction of root. Finally, we realize the visualization expression of plant root, basing on the VTK simulation software. The result of simulation, for establishing the whole visualization plant growing root further, lays technical foundation.

중국 관세행정구제제도의 발전방안에 관한 비교연구

포붕(Peng Bao),한상현(Sang-Hyun Han) 한국관세학회 2014 관세학회지 Vol.15 No.1

Now, China is South Korea’s foreign investor and trading partner in the world while South Korea is China’s third largest trading partner. The trade relation over the Korea-China is consistently deepening and increasing. That’s the main cause of the development of future for the two countries The trade growth between the two countries is that be common to South Korea and China. Naturally, there will be a administration fight between tax payer and tax government office is expected to rise. In there circumstances, we make a judgment thats very important to understand the Customs Administrative Relief System. Customs Administrative Relief System is lexical relief system of administrative measure that is unfair violation of law on tariff. The name is some different in Chinese situation, but the contents have gone Customs Administrative Relief System similar Korea. Customs Administrative Relief System in China is objection system, reexamination system of objection, administrative litigation system, and Administration reparation system. For this reason, in this study, I searched Customs Administrative Relief System of two countries and compared the difference and something in common also we found out which one is more better in a way and which one is more beneficial than others for severing taxpayer. Especially, I made a comparative study between Korea and Chinas Customs Administrative Relief System and locate the problem of China also suggest the improvement alternative of it. So, I hope this study can help the people to recognize the system also can help the policy maker to make amendments to tax-related legislations.

A Substation-Oriented Approach to Optimal Phasor Measurement Units Placement

Bao, Wei,Guo, Rui-Peng,Han, Zhen-Xiang,Chen, Li-Yue,Lu, Min The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.1

State Estimation (SE) is the basis of a variety of advanced applications used in most modern power systems. An SE problem formed with enough phasor measurement units (PMUs) data is simply a linear weighted least squares problem requiring no iterations. Thus, designing a minimum-cost placement of PMUs that guarantees observability of a power system becomes a worthy challenge. This paper proposes an equivalent integer linear programming method for substation-oriented optimal PMU placement (SOOPP). The proposed method uses an exhaustive search to determine a globally optimal solution representing the best PMU placement for that particular power system. To obtain a more comprehensive model, contingencies and the limitation of the number of PMU measurement channels are considered and embodied in the model as changes to the original constraints and as additional constraints. The proposed method is examined for applicability using the IEEE 14-bus, 118-bus and 300-bus test systems. The comparison between SOOPP results and results obtained by other methods reveals the excellence of SOOPP. Furthermore, practical large-scale power systems are also successfully analyzed using SOOPP.

Mesh Generation Based on Virtual Geometry

Peng Wei,Chen Wenliang,Bao Yidong 한국소성가공학회 2010 기타자료 Vol.2010 No.6

Finite element mesh generation algorithm often requires the ability to abstract and modify the topology of a boundary representation model. A method based on virtual geometry for mesh generation is proposed to modify the model topology without changing the geometry of the model. By this method, a tool is given to adjust the model topology for optimal meshing, while leaving the geometry or shape of the model, and the accuracy of the resulting mesh in representing the model, unchanged. Our practical examples show that the proposed method is effective and reliable for mesh generation in molding analysis field.

Anti-adherence of Antibacterial Peptides and Oligosaccharides and Promotion of Growth and Disease Resistance in Tilapia

Peng, K.S.,She, R.P.,Yang, Y.R.,Zhou, X.M.,Liu, W.,Wu, J.,Bao, H.H.,Liu, T.L. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.4

Four hundred and fifty tilapias ($6.77{\pm}0.23$ g) were assigned randomly to six groups to evaluate the feasibility of the tested antibacterial peptides (ABPs) and oligosaccharides as substitutes for antibiotics. The control group was fed with a commercial tilapia diet; other five groups were fed with the same commercial diet supplemented with konjac glucomannan (KGLM), cluster bean galactomannan (CBGAM), and three animal intestinal ABPs derived from chicken, pig and rabbit at 100 mg/kg respectively. After 21 days of feeding, growth, disease resistance, and in vivo anti-adherence were determined. Furthermore, the inhibitory effect of tested agents on adhesion of Aeromonas veronii biovar sobria (A.vbs) strain BJCP-5 to tilapia enteric epithelia in vitro was assessed by cell-ELISA system. As a result, the tested agents supplemented at 100 mg/kg show significant benefit to tilapia growth and disease resistance (p<0.05), and the benefit may be correlated with their interfering in the contact of bacteria with host mucosal surface. Although none of the tested agents did inhibit the growth of BJCP-5 in tryptic soy broth at $100{\mu}g/ml$, all of them did inhibit the adhesion of A.vbs to tilapia enteric epithelia in vivo and in vitro. In vitro mimic assays show that three ABPs at low concentrations of $25{\mu}g/ml$ and $2.5{\mu}g/ml$ have the reciprocal dose-dependent anti-adherence effect. The inhibition of ABPs may be correlated with a cation bridging and/or receptor-ligand binding, but not with hydrophobicity. The KGLM and CBGAM inhibited the adherence of BJCP-5 to tilapia enteric epithelia with dose-dependent manner in vitro, and this may be through altering bacterial hydrophobicity and interfering with receptor-ligand binding. Our results indicate that the anti-adherence of the tested ABPs and oligosaccharides may be one of the mechanisms in promoting tilapia growth and resistance to A.vbs.