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유영복,유창현,Peberdy, John F. 한국균학회 1985 韓國菌學會誌 Vol.13 No.1
This experiment was undertaken to investigate proper conditions for protoplast isolation from strains of Agaricus bisporus, Flammulina velutipes, Lentinus edodes, Pleurotus florida, Pleurotus ostreatus, Pleurotus sajor-caju and Volvariella volvacea. Combination of β-D-glucanase, novozym 234 and snail enzyme with 0.6M MgSO₄ in 0.05M Na-maleate buffer, pH 5.8 was the most effective for isolation of protoplasts. High yields of protoplasts were obtained from 4∼5 days old mycelia of P. florida and P. ostreatus on mushroom complete agar medium. Protoplasts were also obtained in good yield from other fungi but A. bisporus and V. volvacea. In the latter two cases protoplast release was observed; however, the yield was much lower than those of the other fungi.
느타리버섯과 사철느타리버섯의 종간 원형질체융합 균주의 특성
박용환,유영복,유창현,고승주,변명옥,Peberdy, John F. 한국균학회 1984 韓國菌學會誌 Vol.12 No.4
Interspecific heterokaryons were obtained between auxotrophic mutants of Pieurotus ostreatus and Pleurotus florida by polyethylene glycol-Ca^(++) induced fusion of somatic protuplasts. The fusion products produced colonies of dense growing mycelium after 10-14 days culture on hypertonic Mushroom Minimal Medium. When they were transferred to Mushroom Minimal Medium plates, the sectors showed normal vegetative morphology but the colonies of irregular shape varied in growth rate. All of the colonies produced fruit body of normal pilei. Some heterokaryon colonies bearing none or only a small amounts of basidiospores were isolated. Fusion products, generally, gave higher basidiocarp yields than the parents.
느타리버섯과 사철느타리버섯의 원형질체 재생 및 환원에 관한 연구
유영복,차동열,죤페버디 한국균학회 1985 韓國菌學會誌 Vol.13 No.2
The experiment of protoplast regeneration and reversion were undertaken to provide a basic techniques for protoplast manipulation. Protoplasts of Pleurotus florida and Pleurotus ostreatus were reverted to normal hyphal growth and the reversion frequency of both fungal protoplasts were 0.24∼3.19%. Reversion medium stabilized with 0.6 M potassium chloride and sucrose was better than the other stabilized one. The protoplast reversion frequency was increased when various amino acid and vitamin compounds were added to the hypertonic mushroom complete agar medium.
자외선 조사에 의한 느타리버섯과 사철느타리버섯 원형질체의 영양요구성 균주 선발에 관한 연구
박용환,유영복,죤페버디 한국균학회 1985 韓國菌學會誌 Vol.13 No.2
Mutagenesis of protoplast could serve a great potential tool for improvement of strains and genetics in higher fungi. For the isolation of auxotrophic mutants from protoplasts of Pleurotus ostreatus and Pleurotus florida, viability levels of ultraviolet lights were determined. Seven auxotrophs were obtained from protoplasts irradiating UV to give 0.83∼15% survival. The mutants showed a single requirement for each of Arg, Ribo-1, Ribo-2 or Phen for growth. Some of them showed two or three kinds of requirements, Gly Ser, Ade Hypo or Ala Orn Tryp for growth.
원형질 체융합에 의한 느타리버섯과 사철느타리버섯 체세포 잡종의 유전 분석
박용환,유영복,유창현,죤페버디 한국균학회 1985 韓國菌學會誌 Vol.14 No.1
Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.
박용환,장권열,유영복,유창현,이연희,존폐버디 한국균학회 1987 韓國菌學會誌 Vol.15 No.3
Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplaste from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopias protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.
YOO, YOUNG BOK,Go, Seung Joo,Park, Yong Hwan,Byun, Myung Ok,You, Chang Hyun,Peberdy, John F. 한국균학회 1985 韓國菌學會誌 Vol.13 No.2
Interspecific heterokaryons were obtained between auxotrophic mutants of Pleurotus ostreatus and Pleurotus florida by polyethylene glycol-Ca^(2+) induced fusion of somatic protoplasts. The fusion products produced colonies of dense growing mycelium after 10∼14 days culture on hypertonic Mushroom Minimal Medium. When they were transferred to Mushroom Minimal Medium plates, the sectors showed normal vegetative morphology but the colonies of irregular shape varied in growth rate. All of the colonies produced fruit body of normal pilei. Some heterokaryon colonies bearing none or only a small amounts of basidiospores were isolated. Fusion products, generally, gave higher basidiocarp yields than the parents.
유영복,유창현,박용환,죤 페버디,Yoo, Young-Bok,You, Chang-Hyun,Park, Yong-Hwan,Peberdy, John F. The Korean Society of Mycology 1986 韓國菌學會誌 Vol.14 No.1
느타리버섯과 사철느타리버섯의 영양요구성 균주를 원형질체융합에 의하여 이종간융합균주를 선발하였다. 이 체세포잡종을 재배하여 자실체에서 담자포자를 분리하여 life cycle에 대한 유전분석을 한 결과를 요약하면 다음과 같다. 1. $Arg{\times}Ribo-1,\;Arg{\times}Ribo-2,\;Gly\;Ser{\times}Ribo-1$ 그리고 $Gly\;Ser{\times}Ribo-2$ 4개의 융합조합에서 7개의 융합균주를 분석한 결과 모두 유전형질이 분리되었으며 또한 유전자 조환이 일어났다. $Arg{\times}Ribo-1$과 $Arg{\times}Ribo-2$에서는 4종류로 분리되었으며 $Gly\;Ser{\times}Ribo-1$, $Gly\;Ser{\times}Ribo-2$와 같이 대립유전자가 3인자 일때는 8종류로 기대되나 단지 2내지 6종류로만 분리 되었다. 2. 대림유전자의 비에 었어서 $Arg{\times}Ribo-1$과 $Arg{\times}Ribo-2$에서 1:1로 기대되나 Ribo의 분리수가 많은 3:1 으로 분리되었으며, $Gly\;Ser{\times}Ribo-1$과 $Gly\;Ser{\times}Ribo-2$에서는 1:1:1과는 아주 상이한 분리비를 나타내었다. 3. 4개의 융합조합 모두 연관에 속하지 않았으며 조환가는 $38.7{\sim}67.6%$였다. 4. 분리되는 prototrophic recombinants의 균사체에서 clamp connection이 형성되었는데 4개의 융합조합에서 모두 나타났으며 융합균주에 따라 차이가 났는데 $Arg{\times}Ribo-1$의 p5균주는 곁정된 수의 78.5%가 clamp connection을 형성하였다. 또한 이들 균주의 자실체는 정상이었으며 자실체의 색은 느타리버섯과 사철느타리버섯의 중간이었다. Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.
Can a Point-of-Care Troponin I Assay be as Good as a Central Laboratory Assay? A MIDAS Investigation
W. Frank Peacock,Deborah Diercks,Robert Birkhahn,Adam J. Singer,Judd E. Hollander,Richard Nowak,Basmah Safdar,Chadwick D. Miller,Mary Peberdy,Francis Counselman,Abhinav Chandra,Joshua Kosowsky,James N 대한진단검사의학회 2016 Annals of Laboratory Medicine Vol.36 No.5
Background: We aimed to compare the diagnostic accuracy of the Alere Triage Cardio3 Tropinin I (TnI) assay (Alere, Inc., USA) and the PathFast cTnI-II (Mitsubishi Chemical Medience Corporation, Japan) against the central laboratory assay Singulex Erenna TnI assay (Singulex, USA). Methods: Using the Markers in the Diagnosis of Acute Coronary Syndromes (MIDAS) study population, we evaluated the ability of three different assays to identify patients with acute myocardial infarction (AMI). The MIDAS dataset, described elsewhere, is a prospective multicenter dataset of emergency department (ED) patients with suspected acute coronary syndrome (ACS) and a planned objective myocardial perfusion evaluation. Myocardial infarction (MI) was diagnosed by central adjudication. Results: The C-statistic with 95% confidence intervals (CI) for diagnosing MI by using a common population (n=241) was 0.95 (0.91-0.99), 0.95 (0.91-0.99), and 0.93 (0.89-0.97) for the Triage, Singulex, and PathFast assays, respectively. Of samples with detectable troponin, the absolute values had high Pearson (RP) and Spearman (RS) correlations and were RP =0.94 and RS=0.94 for Triage vs Singulex, RP =0.93 and RS=0.85 for Triage vs PathFast, and RP =0.89 and RS=0.73 for PathFast vs Singulex. Conclusions: In a single comparative population of ED patients with suspected ACS, the Triage Cardio3 TnI, PathFast, and Singulex TnI assays provided similar diagnostic performance for MI.