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Panha Pok,오은의,이경욱,강종훈,고부영,김호방,송관정 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.2
This study was conducted to investigate the seed formation potential by the evaluation of microspore development and self- and cross-compatibility through their pollen tube growth response to self and cross pollination in Jeju old local citrus species. Anther and pollen histological analyses were done to observe microspore development for three species including byungkyul, sadoogam, and dangyooza. Pollens of ten citrus species were tested for pollen viability by staining with Lugol’s solution and fluorescein diacetate. Flowers of all species were emasculated and selfand cross-pollinated just before anthesis. Pistils of nine pollinating combinations between the listed three species were collected at 1, 3, 5, 7, and 9 days after pollination for evaluation of a growth pattern of the pollen tube. Pistils of the other combinations were collected at 9 days after pollination for self- and cross-compatibility evaluation. Pistils were prepared by squashing and staining method with aniline blue. Eight pollen developmental stages were recognized and indicated normal development of microspore. These ten citrus species produced high percentage of viable pollens. Pollen tube behavior in compatible and incompatible pollination was distinguished. Pollen tube performance was largely depended on male-female combination. All of these Jeju old local citrus species were male fertile and have the ability to produce enough viable pollens for self- and cross-pollination. All evaluated citrus species were self- and cross-compatible to each other except dangyooza which was self-incompatible.
OTA, HIDETOSHI,HONDA, MASANAO,CHEN, SZU-LUNG,HIKIDA, TSUTOMU,PANHA, SOMSAK,OH, HONG-SHIK,MATSUI, MASAFUMI 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.2
Historical relationships were inferred for the oriental lizards of the genus Takydromus Daudin 1802 (Lacertidae) on the basis of DNA sequences. Of the 17 species currently recognized for the genus, 13 species represented by 42 specimens from 29 localities were examined. Maximum-likelihood and maximum-parsimony analyses of data for 829 aligned sites from parts of the mitochondrial 12S and 16S rRNA gene yielded relationships that, while showing no substantial discrepancy with each other, were strikingly different from a currently prevailing phylogenetic hypothesis from a parsimony analysis of morphological characters. Based on the results of these molecular analyses, supplemented by results of the morphological analysis that offered robust evidence for positions of two additional species (T. khasiensis and T. sylvaticus), the following interrelationships were hypothesized as the most preferred phylogeny : (kuehnei (sexlineatus khasiensis)))tachydromoides ((smaragdinus (sauteri (dorsalis sylvaticus))) (amurensis (((formosanus wolteri) hsuehshanensis)(toyamai (septentrionalis stejnegeri)))))). These interrelationships indicate; (1) invalidity of Platyplacopus Boulenger 1917, which was recently resurrected as a subgenus of Takydromus on the basis of the morphological analysis; (2) homoplasy in states of some morphological characters, such as green dorsal coloration, that were considered as synapomorphs of certain nodes in the morphological analysis; and (3) involvement of the genus in a series of vicariances in both the continental and insular parts of eastern Eurasia. Due to the paucity of available samples, phylogenetic status of T. intermedius and T. haughtonianus remain to be examined in future studies.