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Heterogeneity of CMA Banding Patterns in Jeju Citrus Landraces
Kyunguk Yi,Chi‐Won Chae,Young‐Chul Park,Ho‐Bang Kim,Kwan‐Jeong Song 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
CMA banding patterns of chromosomes of eleven Jeju citrus landraces were characterized and compared by means of sequential staining using fluorochromes of chromomycin A3 (CMA) and 4’,6-diamidino-2-phenylindole (DAPI). The somatic metaphase chromosomes examined in this study were all diploids (2n = 18). Chromosomes were classified into five types based on the number and distribution of CMA positive bands; A: two telomeric and one proximal bands, B: one telomeric and one proximal bands, C: two telomeric bands, D: one telomeric band, E: no band. Four to five types of chromosomes and unique chromosome compositions were observed from each accession. The CMA banding patterns of Jeju citrus landraces were 1A+1B+1C+9D+6E in jinkyul, 1A+1B+1C+8D+7E in cheongkyul, 1B+1C+10D+6E in hongkyul, 2A+1B+3C+6D+6E in sadoogam, 1A+2B+1C+8D+6E in dangyooza, 1A+1B+3C+7D+6E in dong-geongkyul, 2B+2C+7D+7E in pyunkyul, 2A+2B+2C+6D+6E in gamza, 1A+2B+1C+7D+7E in byungkyul, 1A+1B+1C+9D+6E in jigak, 1A+1C+10D+6E in binkyul. Type D and E chromosomes were predominant in all Jeju citrus landraces. The chromosome composition with an even number distribution in gamza was observed, hence it could be recognized as a non-hybrid species. The results indicated all Jeju citrus landraces except gamza seemed to be hybrids, but might be diverged from species originated or cultivated in Jeju, Korea and other countries.
Comparison of Major Component Contents in Black Teas Manufactured from Korea and Foreign Countries
Tethvoleak Srey,Kyunguk Yi,Eun Ui Oh,In Kwan Song,Bong Chan Kim,Jian-Liang Lu,Min Seok Lee,and Kwan Jeong Song 한국차학회 2014 한국차학회지 Vol.20 No.1
홍차의 핵심 성분의 분석을 통해 국가 및 지역에 따라 제조된 제품의 성분적 품질을 평가하고자 본 연구를 수행하였다. 중국 4개, 인도 6개, 일본 3개, 한국 8개 및 스리랑카 7개를 포함한 총 29개 제품이 이용되었다. 총아미노산, 총폴리페놀 및 데아루비긴의 함량은 비색계를, 그리고 카테킨과 데아플라빈은 고속크로마토그래피를 이용하여 분석하였다. 분석결과 이들 성분들은 제조한 국가 및 지역에 따라 큰 차이가 있었다. 특히, 총폴리페놀, 카테킨, 데아플라빈의 성분에서 함량 차이가 크게 나타났다. 스리랑카산 홍차는 가장 높은 데아플라빈 및 데아루비긴 함량을 나타내었다. 인도산에서 가장 높은 총폴리페놀 및 카테킨 함량이 조사되었다. 본 연구결과 성분적 품질에서 스리랑카 홍차와 인도 홍차 제품들은 중국, 일본 및 한국산 제품들보다 우수하였으며, 한국 홍차 제품들은 중국 및 일본산 제품에 비해 떨어지지 않은 것으로 나타났다. The study evaluated the componential quality of black teas manufactured from different countries and regions by analyzing key chemical components in black tea. A total of 29 black teas consisting of four manufactured in China, six in India, three in Japan, eight in Korea, and seven in Sri Lanka were used. The total amino acids (TAAs), total polyphenols (TPPs) and thearubigins (TRs) contents were measured using a spectrophotometer, and the catechins, caffeine and theaflavins (TFs) were measured by high performance liquid chromatography. The contents of these components differed significantly according to the countries of origin or regions manufacturing black tea. In particular, large differences in the TPPs, total catechins and TFs contents were observed. Sri Lankan black tea contained the highest level of TFs and TRs. Indian black tea contained the highest content of TPPs and total catechins. These results suggest that Sri Lankan and Indian black tea is superior to Chinese, Japanese and Korean tea, but Korean black tea is not inferior to Chinese and Japanese in terms of the componential quality.