Biological Properties of (1 → 3)-β-D-Glucan-Based Synthetic Oligosaccharides

Sujata Saraswat-Ohri,Aruna Vashishta,Vaclav Vetvicka,Karine Descroix,Frank Jamois,Jean-Claude Yvin,Vincent Ferrie`res 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.4

Despite the fact that β-glucans are well-established immunomodulators, the problems with batch-to-batch heterogeneity remains problematic. The aim of this study was to prepare and evaluate new type of synthetic oligosaccharides. A new family of oligo-(1 → 3)-β-D-glucans modified on the reducing end was synthesized using a controlled and specific inversion of configuration at C-2 starting from already formed oligo-(1 → 3)-β-D-glucans. The designed glycosides are characterized by the presence of four or five glucopyranose entities and a mannose residue at the reducing end. To study of the impact of well-defined structural modulations, we used murine and human models to evaluate their immunostimulating potential. These novel oligosaccharides showed strong and long-lasting stimulation of phagocytosis and significant potentiation of synthesis and/or secretion of interleukin (IL-2, IL-4, IL-5, IL-6), tumor necrosis factor-α, and vascular endothelial growth factor. In addition, the oligosaccharides tested showed significant effects on expression of several genes in human fibroblasts and breast cancer cells. From our results it is clear that these synthetic oligosaccharides represent a better alternative to natural β-glucans.

Anti-calcification of Bovine Pericardium for Bioprosthetic Heart Valves after Surface Modification with Hyaluronic Acid Derivatives

한세광,Rachit Ohri,Cecilia M. Giachelli 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Surface modification of glutaraldehyde fixed bovine pericardium (GFBP) was successfully carried out with hyaluronic acid (HA) derivatives. At first, HA was chemically modified with adipic dihydrazide (ADH) to introduce hydrazide functional group into the carboxyl group of HA backbone. Then, GFBP was surface modified by grafting HA-ADH to the free aldehyde groups on the tissue and the subsequent HA-ADH hydrogel coating. HA-ADH hydrogels could be prepared through selective crosslinking at low pH between hydrazide groups of HA-ADH and crosslinkers containing succinimmidyl moieties with minimized protein denaturation. When HA-ADH hydrogels were prepared at low pH of 4.8 in the presence of erythropoietin (EPO) as a model protein, EPO release was continued up to 85% of total amount of loaded EPO for 4 days. To the contrary, only 30% of EPO was released from HA-ADH hydrogels prepared at pH=7.4, which might be due to the denaturation of EPO during the crosslinking reaction. Because the carboxyl groups on the glucuronic acid residues are recognition sites for HA degradation by hyaluronidase, the HA-ADH hydrogels degraded more slowly than HA hydrogels prepared by the crosslinking reaction of divinyl sulfone with hydroxyl groups of HA. Following a two-week subcutaneous implantation in osteopontin-null mice, clinically significant levels of calcification were observed for the positive controls without any surface modification. However, the calcification of surface modified GFBP with HA-ADH and HA-ADH hydrogels was drastically reduced by more than 85% of the positive controls. The anti-calcification effect of HA surface modification was also confirmed by microscopic analysis of explanted tissue after staining with Alizarin Red S for calcium, which followed the trend as observed with calcium quantification.

Future Perspective and Current Aspects of Internet of Things Enable Design

Evedeep Kaur Bhatia,Simran Ohri,Gurbani Kaur,Minal Dhankar,Sweety Dabas 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.8

In this work, we are going to survey the latest progress in Internet of Things (IoTs) and also design IoTs enable electronics design like frame buffer, content addressable memory, and key generator for encryption and decryption. We are analyzing future perspective, overall impact, and its role in every corner of life, characteristics features and current aspects of IoTs. Apart from this, we study how this concept came into existence and its emergence changes our lives. In this paper, we have also designed IoTs enable Frame Buffer on FPGA for Object Tracking, IoTs enable Content Addressable Memory for processor and IoTs enable Key Generator for Green Communication. In order to make IOTs enable design, we are embedding a 128-bit Internet Protocol Version 6 (IPv6) address in each and every design that enables.

A STUDY OF SLANT WEIGHTED TOEPLITZ OPERATORS IN CALKIN ALGEBRA

Gopal Datt,NEELIMA OHRI 장전수학회 2018 Proceedings of the Jangjeon mathematical society Vol.21 No.1

We describe some structural properties of a kth-order slant weighted Toeplitz operator Uβκ,Φ(k≥ 2) on L²(β) with Φ∈ L∞(β). The study of this operator and its counterpart on H²(β) is also extended in reference to the Calkin algebra.

Anti-calcification of Bovine Pericardium for Bioprosthetic Heart Valves after Surface Modification with Hyaluronic Acid Derivatives

Hahn Sei Kwang,Ohri Rachit,Giachelli Cecilia M. The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Surface modification of glutaraldehyde fixed bovine pericardium (GFBP) was success­fully carried out with hyaluronic acid (HA) derivatives. At first, HA was chemically modified with adipic dihydrazide (ADH) to introduce hydrazide functional group into the carboxyl group of HA backbone. Then, GFBP was surface modified by grafting HA-ADH to the free aldehyde groups on the tissue and the subsequent HA-ADH hydrogel coating. HA-ADH hydrogels could be prepared through selective crosslinking at low pH between hydrazide groups of HA-ADH and crosslinkers containing succinimmidyl moieties with minimized protein denaturation. When HA­ADH hydrogels were prepared at low pH of 4.8 in the presence of erythropoietin (EPO) as a model protein, EPO release was continued up to $85\%$ of total amount of loaded EPO for 4 days. To the contrary, only $30\%$ of EPO was released from HA-ADH hydrogels prepared at pH=7.4, which might be due to the denaturation of EPO during the crosslinking reaction. Because the carboxyl groups on the glucuronic acid residues are recognition sites for HA degradation by hyaluronidase, the HA-ADH hydrogels degraded more slowly than HA hydrogels prepared by the crosslinking reaction of divinyl sulfone with hydroxyl groups of HA. Following a two-week subcutaneous implantation in osteopontin-null mice, clinically significant levels of calcification were observed for the positive controls without any surface modification. However, the calcification of surface modified GFBP with HA-ADH and HA-ADH hydrogels was drastically reduced by more than $85\%$ of the positive controls. The anti-calcification effect of HA surface modification was also confirmed by microscopic analysis of explanted tissue after staining with Alizarin Red S for calcium, which followed the trend as observed with calcium quantification.

INFLUENCE OF DAILY REPEATED COLD EXPOSURES ON THE TCA CYCLE IN THE SHEEP LIVER

Seto, K.,Kaba, H.,Ohri, A.,Saito, H.,Kimura, F.,Kawakami, M.,Black, A.L. Asian Australasian Association of Animal Productio 1989 Animal Bioscience Vol.2 No.3

Immunological Effects of Yeast- and Mushroom-Derived β-Glucans

Vaclav Vetvicka,Aruna Vashishta,Sujata Saraswat-Ohri,Jana Vetvickova 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.4

Glucans have a long history as nonspecific biological modulators. We compared the effects of three different glucans on immune reactions. Using two different administrations (intraperitoneal and oral) and two different animal models, we showed that yeast-derived Betamune™ (Biorigin, São Paulo, Brazil) caused significant stimulation of phagocytic activity as well as potentiation of synthesis and release of interleukin (IL)-1, IL-2, IL-4, IL-6, IL-8, IL-13, and tumor necrosis factor-α. In addition, Betamune inhibited growth of tumor cells in vivo and affected expression of several important genes in breast cancer cells. Compared to adult mice, young animals showed different sensitivity to glucan action.

Management of symptomatic radiation necrosis after stereotactic radiosurgery and clinical factors for treatment response

Mutlay Sayan,Teuta Zoto Mustafayev,Aykut Balmuk,Swati Mamidanna,Erva Seyma Sare Kefelioglu,Gorkem Gungor,Anupama Chundury,Nisha Ohri,Ercan Karaarslan,Enis Ozyar,Banu Atalar 대한방사선종양학회 2020 Radiation Oncology Journal Vol.38 No.3

Purpose: Approximately 10% of patients who received brain stereotactic radiosurgery (SRS) develop symptomatic radiation necrosis (RN). We sought to determine the effectiveness of treatment options for symptomatic RN, based on patient-reported outcomes. Materials and Methods: We conducted a retrospective review of 217 patients with 414 brain metastases treated with SRS from 2009 to 2018 at our institution. Symptomatic RN was determined by appearance on serial magnetic resonance images (MRIs), MR spectroscopy, requirement of therapy, and development of new neurological complaints without evidence of disease progression. Therapeutic interventions for symptomatic RN included corticosteroids, bevacizumab and/or surgical resection. Patient-reported therapeutic outcomes were graded as complete response (CR), partial response (PR), and no response. Results: Twenty-six patients experienced symptomatic RN after treatment of 50 separate lesions. The mean prescription dose was 22 Gy (range, 15 to 30 Gy) in 1 to 5 fractions (median, 1 fraction). Of the 12 patients managed with corticosteroids, 6 patients (50%) reported CR and 4 patients (33%) PR. Of the 6 patients managed with bevacizumab, 3 patients (50%) reported CR and 1 patient (18%) PR. Of the 8 patients treated with surgical resection, all reported CR (100%). Other than surgical resection, age ≥54 years (median, 54 years; range, 35 to 81 years) was associated with CR (odds ratio = 8.40; 95% confidence interval, 1.27-15.39; p = 0.027). Conclusion: Corticosteroids and bevacizumab are commonly utilized treatment modalities with excellent response rate. Our results suggest that patient’s age is associated with response rate and could help guide treatment decisions for unresectable symptomatic RN.

Clinical management of uveal melanoma: a comprehensive review with a treatment algorithm

Mutlay Sayan,Swati Mamidanna,Damla Oncel,Imraan Jan,Irina Vergalasova,Joseph Weiner,Nisha Ohri,Banu Acikalin,Anupama Chundury 대한방사선종양학회 2020 Radiation Oncology Journal Vol.38 No.3

Uveal melanoma (UM), the most frequently occurring non-cutaneous melanoma and most common primary intraocular malignancy in adults, arises from the melanocytes of the choroid in approximately 95% of cases. Prompt diagnosis and treatment is vital as primary tumor size is one of the key factors associated with survival. Despite recent advances in management, more than half of the patients develop metastatic disease which portends poor survival. Currently, treatment options for UM include local resection, enucleation, plaque brachytherapy, and/or particle beam radiotherapy (RT). Enucleation was initially the standard of care in the management of UM, but a shift towards eye-preserving therapeutic choices such as RT and local resection has been noted in recent decades. Plaque brachytherapy, a form of localized RT, is the most popular option and is now the preferred treatment modality for a majority of UM cases. In this review we discuss the etiopathogenesis, clinical presentation and diagnosis of UM and place a special emphasis on its therapeutic options. Furthermore, we review the current literature on UM management and propose a functional treatment algorithm for non-metastatic disease.

Evaluation of response to stereotactic radiosurgery in patients with radioresistant brain metastases

Mutlay Sayan,Teuta Zoto Mustafayev,Bilgehan Sahin,Erva Seyma Sare Kefelioglu,Shang-Jui Wang,Varsha Kurup,Aykut Balmuk,Gorkem Gungor,Nisha Ohri,Joseph Weiner,Enis Ozyar,Banu Atalar 대한방사선종양학회 2019 Radiation Oncology Journal Vol.37 No.4

Purpose: Renal cell carcinoma (RCC) and melanoma have been considered ‘radioresistant’ due to the fact that they do not respond to conventionally fractionated radiation therapy. Stereotactic radiosurgery (SRS) provides high-dose radiation to a defined target volume and a limited number of studies have suggested the potential effectiveness of SRS in radioresistant histologies. We sought to determine the effectiveness of SRS for the treatment of patients with radioresistant brain metastases. Materials and Methods: We performed a retrospective review of our institutional database to identify patients with RCC or melanoma brain metastases treated with SRS. Treatment response were determined in accordance with the Response Evaluation Criteria in Solid Tumors. Results: We identified 53 radioresistant brain metastases (28% RCC and 72% melanoma) treated in 18 patients. The mean target volume and coverage was 6.2 ± 9.5 mL and 95.5% ± 2.9%, respectively. The mean prescription dose was 20 ± 4.9 Gy. Forty lesions (75%) demonstrated a complete/partial response and 13 lesions (24%) with progressive/stable disease. Smaller target volume (p < 0.001), larger SRS dose (p < 0.001), and coverage (p = 0.008) were found to be positive predictors of complete response to SRS. Conclusion: SRS is an effective management option with up to 75% response rate for radioresistant brain metastases. Tumor volume and radiation dose are predictors of response and can be used to guide the decision-making for patients with radioresistant brain metastases.