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        The Effects of Mortality and Influence of Pheromone Trapping on the Infestation of Oryctes rhinoceros in an Oil Palm Plantation

        Kamarudin, Norman Hj,Wahid, Mohd Basri,Moslim, Ramle,Ali, Siti Ramlah Ahmad Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.3

        The infestation levels of O. rhinoceros were investigated in the oil palm trunk heaps in three replanting blocks (Blocks A, B and C) at Sepang, Selangor, Malaysia. These blocks had different densities of pheromone trapping: high (11 traps per ha, Block B), normal (1 trap per 2 ha, Block C), while no pheromone trapping was conducted in (Block A. Without pheromone trapping, the infestation levels of O. rhinoceros can reach a maximum density of between 25 to 50 individuals per $m^2$ (IPMS). The O. rhinoceros population was still detected in the heaps up to more than two years (at 26 months after replanting). With high density trapping, infestation levels were maintained below 10 IPMS and was not detected in the heaps after a period of 16 months of replanting. In the normal trapping density, the population was also maintained below 10 IPMS but had sustained itself in the heaps for up to 24 months. The mortality factors associated with the larval stages sampled within these replanting blocks was determined by visual symptoms with subsequent verification with microbial analysis. The analysis was narrowed down to detect only bacteria (Bacillus thuringiensis and B. popillae), virus (Oryctes virus) and fungus (Metarhizium). In Block A, the population of O. rhinoceros in the trunks was reduced drastically at 14 months after replanting (MAR), which could be due to the high mortality (88%) of the third instar larvae, 3 months earlier. In Block B, the percentage mortality for the larval stage was always highest at the third ins tars (between 21-82%). In Block C however, the mortality of the third instars was low, never exceeding 35%. In these sampling blocks, the infection of Metarhizium seem to be less dominant compared to virus or bacteria The k factor analysis indicates density dependence among the third instar larvae had contributed to the change in its population density in Block A. Therefore, with proper manipulation of pheromone traps and targeted application of disease agents (i.e virus, fungus and bacteria), the population of O. rhinoceros can be managed more effectively in an oil palm replanting.

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        An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense

        Lee Pei Lee Angel,Mohd Termizi Yusof,Intan Safinar Ismail,Bonnie Tay Yen Ping,Intan Nur Ainni Mohamed Azni,Norman Hj Kamarudin,Shamala Sundram 한국미생물학회 2016 The journal of microbiology Vol.54 No.11

        Ganoderma boninense is the causal agent of a devastating disease affecting oil palm in Southeast Asian countries. Basal stem rot (BSR) disease slowly rots the base of palms, which radically reduces productive lifespan of this lucrative crop. Previous reports have indicated the successful use of Trichoderma as biological control agent (BCA) against G. boninense and isolate T. virens 7b was selected based on its initial screening. This study attempts to decipher the mechanisms responsible for the inhibition of G. boninense by identifying and characterizing the chemical compounds as well as the physical mechanisms by T. virens 7b. Hexane extract of the isolate gave 62.60% ± 6.41 inhibition against G. boninense and observation under scanning electron microscope (SEM) detected severe mycelial deformation of the pathogen at the region of inhibition. Similar mycelia deformation of G. boninense was observed with a fungicide treatment, Benlate® indicating comparable fungicidal effect by T. virens 7b. Fraction 4 and 5 of hexane active fractions through preparative thin layer chromatography (P-TLC) was identified giving the best inhibition of the pathogen. These fractions comprised of ketones, alcohols, aldehydes, lactones, sesquiterpenes, monoterpenes, sulphides, and free fatty acids profiled through gas chromatography mass spectrometry detector (GC/MSD). A novel antifungal compound discovery of phenylethyl alcohol (PEA) by T. virens 7b is reported through this study. T. virens 7b also proved to be an active siderophore producer through chrome azurol S (CAS) agar assay. The study demonstrated the possible mechanisms involved and responsible in the successful inhibition of G. boninense.

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