Haematological and Immunological Response in Lambs Fed on Raw and Variously Processed Cottonseed Meal

Nagalakshmi, D.,Sastry, V.R.B.,Agrawal, D.K.,Katiyar, R.C. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.1

An experiment was conducted with twenty crossbred male lambs to assess the effect of cotton (Gossypium) seed meal (CSM) on blood constituents and immunity. Lambs were randomly assigned to a reference diet (30% deoiled peanut meal, DPNM) and four test diets containing 40% of either raw, 45 minutes cooked, 1% $Ca(OH)_2$ and iron (1 free gossy-pol, FG : 0.3 Fe) treated CSM (replacing approximately 50%, reference concentrate mixture). These isonitrogenous and isocaloric concentrate mixtures were fed to meet 80% of protein requirements (NRC, 1985) along with ad lib maize hay for 180 days. Blood was collected at 60, 120 and 180 days post feeding. The lambs were sensitized with Brucella abortus S99 antigen after 140 days and were subjected to ELISA and delayed type hypersensitivity. Blood haemoglobin, erythrocyte count, leucocyte count, total protein, total albumin, total globulin, urea, creatinine concentration and aspartate aminotransferase activity in lambs fed on raw or processed CSM were comparable to the values of reference lambs. The higher (p<0.01) blood glucose levels observed in CSM fed lambs at 60 days of feeding was latter reduced to the levels comparable with those on reference diet at 120 and 180 days of feeding. The alanine amino transferase activity was lower in lambs fed raw and cooked CSM containing diets at 120 and 180 days of feeding. A marginal increase in serum iron and alkaline posphatase activity was observed in iron treated group and raw CSM fed lambs, respectively. The humoral immune response and DTH reactivity was lower (p<0.05) in lambs fed raw CSM (consuming 302.83 mg FG/day). Cooking, $Ca(OH)_2$ and iron treatment of raw CSM showed a positive response in alleviating the suppression of immune response owing to the reduced consumption of FG by 40.19, 17.40% and 26.73%, respectively in these diets. The present study thus indicated that consumption of 40% raw CSM (302.83 mg FG/day) though did not affect majority of the haematological and blood biochemical parameters, but markedly suppressed the immune mechanism of lambs.

Influence of Feeding Processed Cottonseed Meal on Meat and Wool Production of Lambs

Nagalakshmi, D.,Sastry, V.R.B.,Rao, V. Kesava Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.1

In order to assess the effect of feeding raw or processed cotton (Gossypium) seed meal (CSM) on meat and wool production, 30 male crossbred lambs (3-4 months) of uniform body weight were assigned equally to five dietary treatments in a completely randomised design. The CSM was processed by three different methods i.e., cooking the meal at $100^{\circ}C$ for 45 minutes, treatment with 1% calcium hydroxide ($Ca(OH)_2$) for 24 h and iron treatment in the ratio of 1 part free gossypol (FG) to 0.3 parts of iron for 30 minutes. The lambs were fed isonitrogenous and isocaloric concentrate mixtures, containing 30% deoiled peanut meal (reference diet) and 40% of either raw, cooked, $Ca(OH)_2$ or iron treated CSM for 180 days. The raw and variously processed CSM replaced about 50% nitrogen of reference concentrate mixture. The concentrate mixtures were fed to meet 80% of the protein requirements (NRC, 1985) along with ad libitum chopped maize (Zea mays) hay. The slaughter weight, empty body weight and carcass weight was higher ($p{\leq}0.01$) in lambs fed cooked CSM incorporated diets, compared to diets containing deoiled peanut meal (DPNM). These parameters were not influenced by feeding diets containing either raw, $Ca(OH)_2$ or iron treated CSM in comparison DPNM diets. The carcass length, loin eye area and edible and inedible portion of carcass and the meat: bone ratio in whole carcass were also not affected by feeding CSM based diets. Among various primal cuts, the yield of legs was lower ($p{\leq}0.05$) from raw CSM fed lambs in comparison to DPNM fed lambs. The fat content in the Longissimus dorsi muscle was reduced ($p{\leq}0.05$) in lambs fed processed CSM based diets compared to those fed DPNM diet. Replacing DPNM with either raw or processed CSM based diets did not influence the sensory attributes and overall acceptability of meat. The wool yield was higher ($p{\leq}0.05$) in iron treated CSM fed lambs. The fibre length and fibre diameter were comparable among lambs on various dietary regimes. Among lambs fed variously processed CSM diets, the feed cost per kg of edible meat production was lower ($p{\leq}0.05$) on $Ca(OH)_2$ treated CSM, followed by cooked CSM diet and then on raw CSM based diets compared to DPNM diet. The CSM after 1% $Ca(OH)_2$ treatment or cooking for 45 minutes appears to be a satisfactory protein supplement in lamb diets for meat and wool production to replace at least 50% nitrogen of scarce and costly peanut meal.

Relative Performance of Fattening Lambs on Raw and Processed Cottonseed Meal Incorporated Diets

Nagalakshmi, D.,Sastry, V.R.B.,Agrawal, D.K. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.1

In order to find the feasibility of feeding raw or processed cotton (Gossypium) seed meal (CSM), 30 male crossbred lambs were randomly assigned to 5 dietary treatments with 6 animals each. Lambs were fed each of the isonitrogenous and isocaloric concentrate mixtures containing 30% deoiled groundnut cake (DGNC) (reference diet), 40% raw, 45 minute cooked, 1% $Ca(OH)_2$ or iron (1 part free gossypol:0.3 parts iron) treated CSM (replacing about 50% nitrogen moiety of reference concentrate mixture). The concentrate mixture was fed to meet 80% of CP requirements (NRC, 1985) along with ad libitum maize (Zea mays) hay for 180 days. Incorporation of raw or processed CSM did not affect the palatability of diets as evidenced by higher (p<0.01) or comparable overall daily intakes of DM and protein (CP and Digestible CP) per kg $W^{0.75}$ by lambs in comparison to reference group. Intakes of DE and ME increased (p<0.01) due to inclusion $Ca(OH)_2$ treated CSM in diets, while the intakes were lower (p<0.01) on iron treated CSM based diet when compared with reference diet. The intakes of DM, CP, TDN, DE and ME by reference and experimental lambs were higher than the requirements stipulated by NRC (1985). The growth rate was highest (p<0.01) in lambs fed on diets with cooked CSM followed by those fed raw or $Ca(OH)_2$ treated CSM based diets. The growth of lambs fed on iron treated CSM diets was depressed (p<0.01) in comparison to that of other experimental lambs probably due to increased iron intake (889 mg/kg DM of diet) which was much higher than the toxic levels of 500 ppm (NRC, 1980). The lambs on various diets utilized DM with similar efficiency while the utilization of energy (TDN, DE and ME) was more efficient (p<0.01) when the diets contained raw or cooked CSM in comparison to that of reference diet. The lambs fed raw and $Ca(OH)_2$ treated CSM incorporated diets utilized DCP with higher (p<0.01) efficiency than the lambs fed reference diet. The feed cost per kg weight gain was lower (p<0.01) on raw, cooked and $Ca(OH)_2$ treated CSM based diets in comparison to reference diet. Cooking of CSM for 45 minutes further reduced the feed cost of weight gain.

Development of Appropriate Fibrolytic Enzyme Combination for Maize Stover and Its Effect on Rumen Fermentation in Sheep

Bhasker, T. Vijay,Nagalakshmi, D.,Rao, D. Srinivasa Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.7

In vitro studies were undertaken to develop an appropriate fibrolytic enzymes cocktail comprising of cellulase, xylanase and ${\beta}$-D-glucanase for maize stover with an aim to increase its nutrient utilization in sheep. Cellulase and xylanase added individually to ground maize stover at an increasing dose rates (0, 100, 200, 400, 800, 1,600, 3,200, 6,400, 12,800, 25,600, 32,000, 38,400, and 44,800 IU/g DM), increased (p<0.01) the in vitro dry matter digestibility and in vitro sugar release. The doses selected for studying the combination effect of enzymes were 6,400 to 32,000 IU/g of cellulase and 12,800 to 44,800 IU/g of xylanase. At cellulase concentration of 6,400 IU/g, IVDMD % was higher (p<0.01) at higher xylanase doses (25,600 to 44,800 IU/g). While at cellulase doses (12,800 to 32,000 IU/g), IVDMD % was higher at lower xylanase doses (12,800 and 25,600 IU/g) compared to higher xylanase doses (32,000 to 44,800 IU/g). At cellulase concentration of the 6,400 to 32,000 IU/g, the amount of sugar released increased (p<0.01) with increasing levels of xylanase concentrations except for the concentration of 44,800 IU/g. No effect of ${\beta}$-D-glucanase (100 to 300 IU/g) was observed at lower cellulase-xylanase dose (cellulase-xylanase 12,800 to 12,800 IU/g). Based on the IVDMD, the enzyme combination cellulase-xylanase 12,800 to 12,800 IU/g was selected to study its effect on feed intake and rumen fermentation pattern, conducted on 12 rams (6 to 8 months; $20.34{\pm}2.369$ kg body weight) fed 50% maize stover based TMR. The total volatile fatty acids (p<0.01) and ammonia-N concentration was higher in enzyme supplemented group, while no effect was observed on dry matter intake, ruminal pH and total nitrogen concentration.

Preservers of Gershgorin Set of Jordan Product of Matrices

Joshi, Manoj,Rajeshwari, Kota Nagalakshmi,Santaram, Kilambi,Kanodia, Sandeep Department of Mathematics 2018 Kyungpook mathematical journal Vol.58 No.4

For $A,B{\in}M_2(\mathbb{C})$, let the Jordan product be AB + BA and G(A) the eigenvalue inclusion set, the Gershgorin set of A. Characterization is obtained for maps ${\phi}:M_2(\mathbb{C}){\rightarrow}M_2(\mathbb{C})$ satisfying $$G[{\phi}(A){\phi}(B)+{\phi}(B){\phi}(A)]=G(AB+BA)$$ for all matrices A and B. In fact, it is shown that such a map has the form ${\phi}(A)={\pm}(PD)A(PD)^{-1}$, where P is a permutation matrix and D is a unitary diagonal matrix in $M_2(\mathbb{C})$.

Enhanced membrane fouling control in a hybrid membrane bioreactor with coarse and fine pore sponge pre-filters

S.Mariraj Mohan,S.Nagalakshmi 대한환경공학회 2024 Environmental Engineering Research Vol.29 No.2

Effect of Processing Cotton Straw Based Complete Diet with Expander-extruder on Performance of Crossbred Calves

Kirubanath, K.,Narsimha Reddy, D.,Nagalakshmi, D. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.11

A growth trial of 180 days was conducted on 18 crossbred calves (6-9 months, $73.48{\pm}6.52kg)$ by randomly allotting to two complete diets and a conventional diet (6 in each group). The complete diets were formulated containing 40 per cent cotton straw, one processed in mash form and other subjected to expander-extruder pelletization (EEP). These two complete diets were compared with conventional system of feeding under which concentrate mixture and cotton straw were fed separately in a 60:40 ratio. The calves on EEP complete diet consumed more (p<0.01) DM in comparison to other two groups. The DMI per 100 kg body weight was similar among all the diets. The ADG was significantly (p<0.01) higher in calves fed EEP complete diets (815.4 g) followed by mash (627.0 g) in comparison to conventional diet (464.9 g). The DM intake per kg metabolic body weight was higher (p<0.01) on complete diet than conventional diet. The intakes of DCP (p<0.05), TDN (p<0.01), and ME (p<0.01) per kg metabolic body weight were significantly higher on EEP complete diet in comparison to mash and conventional diet. The water intake per kg DM intake was comparable among all the diets. The efficiency of DM utilisation was higher p (<0.05) on EEP complete diet (5.84) in comparison to conventional diet (7.41), whereas on mash diet it was intermediate (6.68). The efficiency of DCP utilization was similar in mash and EEP complete diet fed groups, which was higher (p<0.05) than that of the conventional diet. Expander-extrusion though increased the cost of production it reduced the cost of feed per unit live weight gain by 12.28% in comparison to its mash form and by 16.76% when concentrate and cotton straw were fed separately. The results indicated that blending of cotton straw along with concentrates in a complete diet increased the palatability of the straw in comparison to conventional system and expander extruder processing of cotton straw based complete diet gave better growth performance and may form an economic ration for growing crossbred calves.

Systematic investigations on the magnetic properties of Gd3Ni2In4 compound

K. Arun,T.P. Rashid,Marian Reiffers,Andrea Dzubinska,R. Nagalakshmi 한국물리학회 2020 Current Applied Physics Vol.20 No.2

We have investigated the structural, magnetic, magnetocaloric, thermodynamic and transport properties of polycrystalline Gd3Ni2In4 compound. X-ray powder diffraction pattern shows that Gd3Ni2In4 crystallizes in hexagonal Lu3Co2In4 – type structure. Magnetization studies reveal the presence of two magnetic transitions, TN and TC at 21 K and 55.5 K, respectively. The maximal value of magnetic entropy change, -ΔSM, computed from isothermal magnetization data in a magnetic field of 9 T is 4.57 J/kg K, which is spread over a wide temperature (ΔT = 61.5 K) and hence yields a relative cooling power (RCP) of 281 J/kg. In addition, the compound shows a significant positive magnetoresistance, MR (T = 2 K) = 44% in B = 9 T. These results on Gd3Ni2In4 compound signify that such materials exhibiting successive reversible magnetic transitions may comprise a distinct class of magnetocaloric materials as they work in a wider temperature range than conventional refrigerant materials.

Enhancement of performance and anti-oxidant variables in broiler chicken fed diets containing sub-optimal methionine level with graded concentrations of sulphur and folic acid

Savaram, Venkata Rama Rao,Mantena, Venkata Lakshmi Narasimha Raju,Paul, Shyam Sunder,Devanaboyina, Nagalakshmi,Thota, Srilatha,Bhukya, Prakash,Ullengala, Rajkumar Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.5

Objective: An experiment was conducted to determine the effects of supplementing graded concentrations of inorganic sulphur (S) without and with folic acid (FA) in maize-soybean meal diets on performance, slaughter and anti-oxidant variables, immune responses and serum protein fractions in broiler chicken. Methods: Inorganic S was supplemented at 0.05%, 0.10%, 0.15%, and 0.20% alone or in combination with FA (4 mg/kg) in basal diet (BD) containing no supplemental methionine (Met) and FA. A control group was fed with the recommended concentration of Met. Each diet was offered to 10 pens of 5 male broiler chicks (Cobb 400) and fed ad libitum from day 1 to 42. Results: The broilers fed the BD had lower body weight gain (BWG), feed efficiency (FE), higher lipid peroxidation (LP), lower activity of glutathione peroxidase (GSHPx), lower lymphocyte proliferation ratio (LPR), and reduced concentrations of total protein, albumin, and globulin in serum. Supplementation of FA and S to the BD improved the BWG (all concentrations of S) and FE (0.20% S) similar to the control group. Similarly, the combination of S and FA significantly improved the concentrations of total protein, albumin, and globulin in serum, reduced the LP and increased the activity of GSHPx and LPR. However, responses in the above parameters were related to the concentration of S in the diet. The slaughter variables and antibody titres against the Newcastle disease were not affected with the treatments. Conclusion: Based on the results, it is concluded that the combination of S (0.2%) and FA (4 mg/kg) improved the BWG and FE, similarly supplementation of these nutrients improved the concentration of protein fractions and reduced the stress (reduced LP and improved GSHPx) variables in serum and improved the cell mediated immune response (LPR) in broilers fed sub-optimal concentrations of Met in diet.

Effect of methyl donors supplementation on performance, immune responses and anti-oxidant variables in broiler chicken fed diet without supplemental methionine

Savaram Venkata Rama Rao,Raju Mantena Venkata Lakshmi Narasimha,Bhukya Prakash,Paul Shyam Sunder,Devanaboyina Nagalakshmi 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.3

Objective: Methionine (Met) is involved in methyl group transfer besides protein synthesis. As the availability is limited and cost is high for synthetic Met, reductions in its inclusion in broiler diet may be possible by supplementing the low Met diets with methyl donors (MD) like betaine (Bet), folic acid (FA), vitamin B12 (B12), and biotin (Bio). An experiment was conducted to study the effects of supplementing the MD on performance (average daily gain [ADG], daily feed intake, feed efficiency [FE]), anti-oxidant variables, immune responses and serum protein concentration in broilers fed sub-optimal concentrations of dietary Met. Methods: Maize-soybean meal diet was used as control (CD). Different MD like Bet (0.2%), B12 (0.1 mg), FA (4 mg), or Bio (1.5 mg/kg) were supplemented to basal diet (BD) having no supplemental Met. The BD without MD was kept for comparison. Each diet was fed ad libitum to 10 replicates of 25 chicks in each from 1 to 42 d of age. Results: At the end of experiment, the ADG in MD group was higher than BD and lower than CD. The FE improved with FA or Bet compared to the BD. Breast meat weight was higher in Bet compared to the BD, while it was intermediate between BD and CD in other groups. The lipid peroxidation reduced with Bio, B12, or Bet, while the glutathione peroxidase activity improved with Bio or B12 compared to the BD. Lymphocyte proliferation improved with Bet compared to the BD. The serum protein concentrations increased with FA, Bio, or Bet compared to those fed BD. Conclusion: It can be concluded that the ADG can be improved partially with supplementation of MD while the FE improved with FA or Bet. Some MD also reduced the stress indices and improved immune responses compared to the BD fed broilers. Objective: Methionine (Met) is involved in methyl group transfer besides protein synthesis. As the availability is limited and cost is high for synthetic Met, reductions in its inclusion in broiler diet may be possible by supplementing the low Met diets with methyl donors (MD) like betaine (Bet), folic acid (FA), vitamin B12 (B12), and biotin (Bio). An experiment was conducted to study the effects of supplementing the MD on performance (average daily gain [ADG], daily feed intake, feed efficiency [FE]), anti-oxidant variables, immune responses and serum protein concentration in broilers fed sub-optimal concentrations of dietary Met.Methods: Maize-soybean meal diet was used as control (CD). Different MD like Bet (0.2%), B12 (0.1 mg), FA (4 mg), or Bio (1.5 mg/kg) were supplemented to basal diet (BD) having no supplemental Met. The BD without MD was kept for comparison. Each diet was fed ad libitum to 10 replicates of 25 chicks in each from 1 to 42 d of age.Results: At the end of experiment, the ADG in MD group was higher than BD and lower than CD. The FE improved with FA or Bet compared to the BD. Breast meat weight was higher in Bet compared to the BD, while it was intermediate between BD and CD in other groups. The lipid peroxidation reduced with Bio, B12, or Bet, while the glutathione peroxidase activity improved with Bio or B12 compared to the BD. Lymphocyte proliferation improved with Bet compared to the BD. The serum protein concentrations increased with FA, Bio, or Bet compared to those fed BD.Conclusion: It can be concluded that the ADG can be improved partially with supplementation of MD while the FE improved with FA or Bet. Some MD also reduced the stress indices and improved immune responses compared to the BD fed broilers.