Some Desmids from Garhwal Region of Uttarakhand, India

Misra, Pradeep Kumar,Misra, Purnima,Shukla, Madhulika,Prakash, Jai The Korean Society of Phycology 2008 ALGAE Vol.23 No.3

The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’to 29° 59’N latitude and 68° 5’to 68° 30’E longitude covering about 2360 km2 area and Dehradun district is situated between 77° 34’to 78° 18’E longitude and 29° 58’to 30° 58’N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.

Improvement in Clonal Propagation of Hemidesmus indicus R. Br. through Adenine Sulphate

Misra Neeta,Misra Pratibha,Datta S.K.,Mehrotra Shanta The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.4

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.

Helicobacter pylori Infection and a P53 Codon 72 Single Nucleotide Polymorphism: a Reason for an Unexplained Asian Enigma

Pandey, Renu,Misra, Vatsala,Misra, Sri Prakash,Dwivedi, Manisha,Misra, Alok Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Aim: P53, the most commonly mutated tumor suppressor gene in all types of human cancer, is involved in cell cycle arrest and control of apoptosis. Although p53 contains several polymorphic sites, the codon 72 polymorphism is by far more common. There are divergent reports but many studies suggest p53 pro/pro SNP may be associated with susceptibility to developing various cancers in different regions of the world. The present study aimed to find any correlation between H. pylori infection and progression of carcinogenesis, by studying apoptosis and the p53 gene in gastric biopsies from north Indian population. Materials and Methods: A total of 921 biopsies were collected and tested for prevalence of H. pylori by rapid urease test (RUT), imprint cytology and histology. Apoptosis was studied by the TUNEL method. Analysis of p53 gene polymorphism at codon 72 was accomplished by PCR using restriction enzyme BstU1. Observation: Out of 921 samples tested 56.7% (543) were H. pylori positive by the three techniques. The mean apoptotic index (AI) in the normal group was 2.12, while gastritis had the maximum 4.24 followed by gastric ulcer 2.28, gastropathy 2.22 and duodenal ulcer 2.08. Mean AI in cases with gastric cancer (1.72) was less than the normal group. The analysis of p53 72 SNP revealed that p53 (Arg/Arg), (Pro /Arg) variant are higher (40.59% & 33.66%) as compared to p53 pro/pro variant (25.74%) inthe healthy population. Conclusions: The North Indian population harbors Arg or Pro/Arg SNP that is capable of withstanding stress conditions; this may be the reason of low incidence of gastric disease in spite of high infection with H. pylori. There was no significant association with H. pylori infection and AI. However, there is increased apoptosis in gastritis which may occur independent of H. pylori or p53 polymorphism.

Some Desmids from Garhwal Region of Uttarakhand, India

Pradeep Kumar Misra,Madhulika Shukla and Jai Prakash,Purnima Misra 한국조류학회I 2008 ALGAE Vol.23 No.3

The present paper consists of 42 taxa belonging to 7 genera of desmids (green algae) collected from two districts of Garhwal region of Uttarakhand (Western Himalayas). The district Haridwar is located 29° 55’ to 29° 59’ N latitude and 68° 5’ to 68° 30’ E longitude covering about 2360 km2 area and Dehradun district is situated between 77°34’ to 78° 18’ E longitude and 29° 58’ to 30° 58’ N latitude. Seven genera of desmids are (with number of taxa in parenthesis): Closterium Nitzsch. (9), Cosmarium Corda ex Ralfs (25), Euastrum Ehr. (2), Spondylosium Breb. (1), Micrasterias Ag. (1), Staurastrum Meyen (3), Arthrodesmus Ehr. (1). All these taxa constitute new records for the area. The algal localities are relatively cleaner than those of majority of urban areas. A rich assemblage of desmids shows that water bodies of these hilly areas are still undisturbed and need protection for preservation of algal biodiversity.

Image-Guided Fine Needle Cytology with Aspiration Versus Non-Aspiration in Retroperitoneal Masses: Is Aspiration Necessary?

Rajiv Kumar Misra,Shaila Mitra,Rishav Kumar Jain,Shilpa Vahikar,Archana Bundela,Purak Misra 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.2

Background: Although using fine needle cytology with aspiration (FNC-A) for establishing diagnoses in the retroperitoneal region has shown promise, there is scant literature supporting a role of non-aspiration cytology (FNC-NA) for this region. We assessed the accuracy and reliability of FNC-A and FNC-NA as tools for preoperative diagnosis of retroperitoneal masses and compared the results of both techniques with each other and with histopathology. Methods: Fifty-seven patients with retroperitoneal masses were subjected to FNC-A and FNC-NA. Smears were stained with May-Grunwald Giemsa and hematoxylin and eosin stain. An individual slide was objectively analysed using a point scoring system to enable comparison between FNC-A and FNC-NA. Results: By FNC-A, 91.7% accuracy was obtained in cases of retroperitoneal lymph node lesions followed by renal masses (83.3%). The diagnostic accuracy of other sites by FNC-A varied from 75.0%–81.9%. By FNC-NA, 93.4% diagnostically accurate results were obtained in the kidney, followed by 75.0% in adrenal masses. The diagnostic accuracy of other sites by FNC-NA varied from 66.7%–72.8%. Conclusions: Although both techniques have their own advantages and disadvantages, FNC-NA may be a more efficient adjuvant method of sampling in retroperitoneal lesions.

A NOTE ON TREE-LIKE SPACES AND THE REGULAR WALLMAN PROPERTY

Misra, K. Department of Mathematics 1982 Kyungpook mathematical journal Vol.22 No.1

Atmospheric pressure cold plasma improves viscosifying and emulsion stabilizing properties of xanthan gum

Misra, N.N.,Yong, Hae In,Phalak, Rohit,Jo, Cheorun Elsevier 2018 Food hydrocolloids Vol.82 No.-

<P><B>Abstract</B></P> <P>In this work, we report the potential application of cold plasma technology for tailoring the functional properties of hydrocolloids. On treating xanthan gum in solid phase with atmospheric air cold plasma, we observed an increase in the viscosifying ability at low shear rates, and emulsion stabilizing activity of the gum, without affecting the colour or the basic polysaccharide backbone. The observed changes have potential benefits for salad-dressing and instant dry soup formulations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effect of cold plasma on properties of xanthan gum is evaluated. </LI> <LI> Plasma treatment of xanthan powder increases low-shear rate viscosity. </LI> <LI> The resulting xanthan has improved emulsion stabilizing capacity. </LI> <LI> The polysaccharide structure remains intact after plasma treatment. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Optimization of Solid State Fermentation of Mustard (Brassica campestris) Straw for Production of Animal Feed by White Rot Fungi (Ganoderma lucidum)

Misra, A.K.,Mishra, A.S.,Tripathi, M.K.,Prasad, R.,Vaithiyanathan, S.,Jakhmola, R.C. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.2

The objective of the experiment was to determine the optimum cultural [moisture levels (55, 60 and 70%), days of fermentation (7, 14 and 21), temperature (25 and $35^{\circ}C$) of incubation)] and nutritional parameters (urea addition (0 and 2%) and variable levels of single super phosphate (0.25 and 0.50% SSP)) for bio-processing of the mustard (Brassica campestris) straw (MS) under solid-state fermentation (SSF) system. The performance of SSF was assessed in terms of favorable changes in cell wall constituents, protein content and in vitro DM digestibility of the MS. Sorghum based inoculum (seed culture) of Ganoderma lucidum to treat the MS was prepared. The 50 g DM of MS taken in autoclavable polypropylene bags was mixed with a pre-calculated amount of water and the particular nutrient in the straw to attained the desired levels of water and nutrient concentration in the substrate. A significant progressive increase in biodegradation of DM (p<0.001), NDF (p<0.01) and ADF (p<0.05) was observed with increasing levels of moisture. Among the cell wall constituents the loss of ADF fraction was greatest compared to that of NDF. The loss of DM increased progressively as the fermentation proceeded and maximum DM losses occurred at 28 days after incubation. The protein content of the treated MS samples increased linearly up to the day $21^{th}$ of the incubation and thereafter declined at day $28^{th}$, whereas the improvement in in vitro DM digestibility were apparent only up to the day $14^{th}$ of the incubation under SSF and there after it declined. The acid detergent lignin (ADL) degradation was slower during the first 7 days of SSF and thereafter increased progressively and maximum ADL losses were observed at the day $28^{th}$ of the SSF. The biodegradation of DM and ADL was not affected by the variation in incubation temperature. Addition of urea was found to have inhibitory effect on fungal growth. The effect of both the levels (0.25 and 0.50) of SSP addition in the substrate, on DM, NDF, ADF, cellulose and ADL biodegradation was similar. Similarly, the protein content and the in vitro DM digestibility remain unaffected affected due to variable levels of the SSP inclusion in the substrate. From the results it may be concluded that the incubation of MS with 60 percent moisture for 21 days at $35^{\circ}C$ with 0.25 percent SSP was most suitable for MS treatment with Ganoderma lucidum. Maximum delignification, enrichment in the protein content and improvement in in vitro DM digestibility were achieved by adopting this protocol of bioprocessing of MS.

Isolation and Characterization of Steroids of Nutraceutical Value in Physalis minima

Misra Laxmi N.,Lal Pyare,Kumar Devinder The Korean Society of Food Science and Nutrition 2006 Preventive Nutrition and Food Science Vol.11 No.2

There is great demand of nutraceutical secondary metabolites in the world so as to feed the population by improving agricultural production with new varieties of food crops but what is still more important is to add nutrition into the food. Physalis minima, plant of Solanaceae family, contain certain secondary metabolites which enhance the over all metabolic functions of the body. Withasteroids are one of such phytochemicals that are generally regarded as safe. These chemicals are almost monopoly of the plants of Solanaceae. The leaves, stem and roots of P. minima have been examined to yield several steroids, identification of which has been discussed in this paper. These withasteroids contribute to the potential nutraceutical and health function value since incorporation of withanolides in the diet may prevent or decrease the growth of tumors in humans.

Eco-friendly method of fabricating indium-tin-oxide thin films using pure aqueous sol-gel

Misra, Mrinmoy,Hwang, Deuk-Kyu,Kim, Yoon Cheol,Myoung, Jae-Min,Lee, Tae Il Elsevier 2018 CERAMICS INTERNATIONAL Vol.44 No.3

<P><B>Abstract</B></P> <P>An indium-tin-oxide (ITO) thin film with approximately 50 nm thickness was successfully synthesized on glass substrates by using a fully aqueous sol-gel process. The sol was prepared from indium nitrate hydrate and tin fluoride as a precursor. Thermogravimetric analysis confirmed that the sol converted into crystalline ITO at 286 °C. The optical band gap and transmittance of the thin film were observed to increase with annealing temperature and plasma treatment time. X-ray photoelectron spectroscopy and transmittance studies established that the number of oxygen vacancies in the thin film drastically increased with increasing temperature and plasma treatment. The annealing temperature and argon plasma treatment time appear to be key factors in reducing resistivity and increasing the transmittance of the thin film. A considerable decrease in the resistivity of the ITO thin film was observed after Ar plasma treatment. This eco-friendly sol-gel ITO thin film may find potential applications in n-type ohmic electrodes for ink-jet printable electronics.</P>