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Minh Tam Le,Hiep Tuyet Thi Nguyen,Trung Van Nguyen,Thai Thanh Thi Nguyen,Hong Nhan Thi Dang,Thuan Cong Dang,Quoc Huy Vu Nguyen The Korean Society for Reproductive Medicine 2023 Clinical and Experimental Reproductive Medicine Vol.50 No.2
Objective: This study aimed to compare the efficacy of physiological intracytoplasmic sperm injection (PICSI) and intracytoplasmic sperm injection (ICSI) in terms of the fertilization rate and embryo quality using sibling oocyte cycles. Methods: This prospective, cross-sectional study collected data from 76 couples who underwent their first cycle at the Hue Center for Reproductive Endocrinology and Infertility, Vietnam, between May 2019 and November 2021. The inclusion criteria were cycles with at least eight oocytes and a sperm concentration of 5×10<sup>6</sup>/mL. Sperm parameters, sperm DNA fragmentation (SDF), fertilization, and the quality of cleavage-stage embryos on day 2 and blastocysts on day 5 were examined. Results: From 76 ICSI cycles, 1,196 metaphase II (MII) oocytes were retrieved, half of which were randomly allocated to either the PICSI (n=592) or ICSI (n=604) treatment group. The results showed no significant difference between the two groups in terms of fertilization (72.80% vs. 75.33%, p=0.32), day 2 cleavage rate (95.13% vs. 96.04%, p=0.51), blastulation rate (52.68% vs. 57.89%), and high-quality blastocyst rate (26.10% vs. 31.13%, p=0.13). However, in cases where SDF was low, 59 cycles consisting of 913 MII oocytes produced a considerably higher blastulation rate with PICSI than with ICSI (50.49% vs. 35.65%, p=0.00). There were no significant differences between the pregnancy outcomes of the PICSI and ICSI embryo groups following embryo transfer. Conclusion: Using variable sperm quality provided no benefit for PICSI versus ICSI in terms of embryo outcomes. When SDF is low, PICSI appears to be able to produce more blastocysts.
Minh Tam Le,Vu Quoc Huy Nguyen,Quang Vinh Truong,Dinh Duong Le,Viet Nguyen Sa Le,Ngoc Thanh Cao 대한내분비학회 2018 Endocrinology and metabolism Vol.33 No.4
Background: Polycystic ovarian syndrome (PCOS) is one of the most common endocrinopathies among reproductive-age women. Its metabolic features often overlap with those associated with metabolic syndrome (MS) and insulin resistance syndrome (IRS). Theobjective of this study was to determine the prevalence and predictors of MS and IRS in infertile Vietnamese women with PCOS. Methods: A cross-sectional study was conducted at a tertiary fertility centre at Hue University Hospital from June 2016 to November 2017. A total of 441 infertile women diagnosed with PCOS based on the revised 2003 Rotterdam consensus criteria were enrolled. MS and IRS were defined based on the National Heart, Lung, and Blood Institute/American Heart Association Adult Treatment Panel III 2005 and American College of Endocrinology IRS 2003 criteria, respectively. Complete clinical and biochemicalmeasurements of 318 women were available for analysis. Independent predictors of MS and IRS were identified using multivariatelogistic regression. Results: The overall prevalence of MS and IRS in women with PCOS was 10.4% and 27.0%, respectively. We identified older age(>30 years) and obesity as independent predictors of MS and IRS. Elevated anti-Müllerian hormone levels increased the risk of IRS,but not that of MS. Conclusion: MS and IRS are prevalent disorders among infertile Vietnamese women with PCOS. PCOS is not solely a reproductiveproblem. Screening and early intervention for MS and/or IRS based on anthropometric, metabolic, and reproductive hormone riskfactors should be an integral part of fertility care.
Does conventional freezing affect sperm DNA fragmentation?
Le, Minh Tam,Nguyen, Thai Thanh Thi,Nguyen, Tung Thanh,Nguyen, Trung Van,Nguyen, Tam An Thi,Nguyen, Quoc Huy Vu,Cao, Thanh Ngoc The Korean Society for Reproductive Medicine 2019 Clinical and Experimental Reproductive Medicine Vol.46 No.2
Objective: Sperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in changes in membrane lipid composition and acrosome status, as well as reductions in sperm motility and viability. This study aimed to evaluate sperm DNA fragmentation damage caused by conventional freezing using the sperm chromatin dispersion test. Methods: In total, 120 fresh human semen samples were frozen by conventional methods, using SpermFreeze Solution as a cryoprotectant. Routine semen analysis and a Halosperm test (using the Halosperm kit) were performed on each sample before freezing and after thawing. Semen parameters and sperm DNA fragmentation were compared between these groups. Results: There was a significant decrease in sperm progressive motility, viability, and normal morphology after conventional freezing (32.78%, 79.58%, and 3.87% vs. 16%, 55.99%, and 2.55%, respectively). The sperm head, midpiece, and tail defect rate increased slightly after freezing. Furthermore, the DNA fragmentation index (DFI) was significantly higher after thawing than before freezing (19.21% prior to freezing vs. 22.23% after thawing). Significant increases in the DFI after cryopreservation were observed in samples with both normal and abnormal motility and morphology, as well as in those with normal viability. Conclusion: Conventional freezing seems to damage some sperm parameters, in particular causing a reduction in sperm DNA integrity.
Effect of Steel Slag on Self-healing Performance of Asphalt Concrete Using Microwave Technique
Tam Minh Phan,Dae-Wook Park,Tri Ho Minh Le,Namyu Kim,Allistair Bliss Tam 한국도로학회 2018 한국도로학회 학술대회 발표논문 초록집 Vol.2018 No.05
This paper evaluates the affection of steel slag in the asphalt mixtures for the self-healing purpose through microwave heating technique. Four different contents of steel wool fibers (SWF) were developed in hot mix asphalt and two types of aggregate were used: steel slag aggregate and normal aggregate. By using the infrared camera, the thermal distribution, as well as the optimum healing time of test samples, were recorded. All mixtures were evaluated their healing performance throughout 8 cycles. The substitution of about 30% normal coarse aggregate by steel slag is prominent due to its effect not only produces better healing performance but also enhances the whole mixture improve load-displacement trend with higher ductile behavior. The application of steel slag in HMA is a promising method which contributes toward the sustainable roadway development.
Le, Minh Tam,Nguyen, Thi Tam An,Nguyen, Thi Thai Thanh,Nguyen, Van Trung,Le, Dinh Duong,Nguyen, Vu Quoc Huy,Cao, Ngoc Thanh,Aints, Alar,Salumets, Andres The Korean Society for Reproductive Medicine 2018 Clinical and Experimental Reproductive Medicine Vol.45 No.3
Objective: In frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET). Methods: Patients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole $40{\mu}m$ in diameter was made in the drilling group. Results: A total of 171 in vitro fertilization/intracytoplasmic sperm injection FET cycles, including 85 cycles with drilling LAH and 86 cycles with thinning LAH, were carried out. The thinning group had a similar ${\beta}$-human chorionic gonadotropin-positive rate (38.4% vs. 29.4%), implantation rate (16.5% vs. 14.4%), clinical pregnancy rate (36.0% vs. 25.9%), miscarriage rate (5.8% vs. 2.4%), ongoing pregnancy rate (30.2% vs. 23.5%), and multiple pregnancy rate (7.0% vs. 10.6%) to the drilling LAH group. There were no significant differences in pregnancy outcomes between subgroups defined based on age (older or younger than 35 years) or ZP thickness (greater or less than $17{\mu}m$) according to the LAH method. Conclusion: The present study demonstrated that partial ZP thinning or drilling resulted in similar outcomes in implantation and pregnancy rates using thawed embryos, irrespective of women's age or ZP thickness.
Crack healing performance of hot mix asphalt containing steel slag by microwaves heating
Phan, Tam Minh,Park, Dae-Wook,Le, Tri Ho Minh Elsevier 2018 Construction and Building Materials Vol.180 No.-
<P><B>Abstract</B></P> <P>This study aims to analyze the applicability of steel slag in the asphalt mixtures for the self-healing purpose using microwave heating technique. Hot mix asphalt (HMA) mixtures were developed from four different levels of steel wool fibers (SWF) as a conductive additive and two types of aggregate were used: steel slag aggregate and conventional aggregate. Then, the thermal distribution, as well as the optimum healing time of test samples, were recorded by the infrared camera. 10 damage-healing cycles were applied in all mixtures to evaluate their healing performance under microwave heat. Test results suggested that adding two percent SWF by weight of asphalt mixture provides the best healing level for both types of aggregate mixtures. At this SWF level, the substitution of 30% normal coarse aggregate by steel slag is very promising due to its presence not only provides better healing results but also helps the whole mixture improve the load-displacement relationship with higher ductile behavior. Overall, the application of steel slag in HMA is a prominent solution which contributes toward the sustainable development.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Steel slag shows better microwave absorption compared to the conventional aggregate. </LI> <LI> Under microwave heating, adding lower than 2% SWF is optimum for healing of HMA. </LI> <LI> Mixtures show ductile behavior at early cycles and brittle characteristics at late times. </LI> <LI> Slag mixture obtain better healing performance compared to the original aggregate. </LI> <LI> The crack formation affects the healing effectiveness. </LI> </UL> </P>
Thi Le Anh,Tan Man Minh,Tung Do Hoang,Tam Do Quang,Hoa Nguyen Minh 한국물리학회 2022 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.80 No.9
A first-principles calculation has investigated the structural, electronic, optical of the CdSe nanocrystal (NC) based on the generalized gradient approximation (GGA) method within the density functional theory. The geometric optimization of the unit cell is in good agreement with our experimental data. The band structure, density of states of the NC were analyzed. The results found that CdSe NC is a direct bandgap with an estimated 1.48 eV. We also report the results of dielectric functions and the refractive index (n) characterized. It is indicated that CdSe materials have excellent photoelectric properties potential applications in optoelectronic devices.