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      • Remedial efficiency study of microbial agents onto contaminated bay sediment of the southeast coast of Korea

        Md. Mahabub Alam,Md. Niamul Haque,Sung-hyun Kwon 한국해양환경·에너지학회 2015 한국해양환경공학회 학술대회논문집 Vol.2015 No.5

        The purposes of this study are to measure environmental condition of the contaminated area and to evaluate remedial efficiency of microbial agents in the contaminated sediment. For this purpose sediments were collected from two different locations, dangdong and danghangpho in the wonmun bay, southeast coast of Korea. Three microbial agents such as Bacillus Bacteria (A), mixture of montmorillonite (B) and biogranula (C) were applied onto contaminated bay sediment to activate microbial population. Geochemical parameters such as chemical oxygen demand (CODMn), total phosphorus (T-P), total nitrogen (T-N), and acid volatile sulfide (AVS) were measured to know environmental condition of the contaminated area. The remedial efficiency of microbial agents was calculated with compared to control sediment within 28 days. The bacterial growth of microbial agents was observed by the total viable count (TVC) method. Those microbial agents were found to reduce chemical oxygen demand (CODMn), total phosphorus (T-P), total nitrogen (T-N), and acid volatile sulfide (AVS) value. Pollutant removal efficiency order of microbial agents were found as microbial agent A > microbial agent B > microbial agent C. These results showed that microbial agent A can reduce chemical oxygen demand (CODMn), total phosphorus (T-P), total nitrogen (T-N), and acid volatile sulfide (AVS) value in the contaminated sediments. Microbial agent A is available in market, easy to spray and comparatively low cost. Therefore the Bacillus Bacteria is a promising agent for remediation of contaminated sediments.

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        Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species

        Md. Mahabub Alam,Masayuki Fujita,Kamrun Nahar,Mirza Hasanuzzaman 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.3

        This study examined the ability of jasmonicacid (JA) to enhance drought tolerance in different Brassicaspecies in terms of physiological parameters, antioxidantsdefense, and glyoxalase system. Ten-day-oldseedlings were exposed to drought (15 % polyethyleneglycol, PEG-6000) either alone or in combination with0.5 mM JA. Drought significantly increased lipoxygenaseactivity and oxidative stress, levels of malondialdehyde andH2O2. Drought reduced seedling biomass, chlorophyll (chl)content, and leaf relative water content (RWC). Droughtincreased proline, oxidized ascorbate (DHA) and glutathionedisulfide (GSSG) levels. Drought affected differentspecies differently: in B. napus, catalase (CAT) and glyoxalaseII (Gly II) activities were decreased, while glutathione-S-transferase (GST) and glutathione peroxidase(GPX) activities were increased in drought-stressed comparedto unstressed plants; in B. campestris, activities ofglutathione reductase (GR), glyoxalase I (Gly I), GST, andGPX were increased, monodehydroascorbate reductase(MDHAR), dehydroascorbate reductase (DHAR), CAT andother enzymes were decreased; in B. juncea, activities ofascorbate peroxidase, GR, GPX, Gly I were increased; GlyII activity was decreased and other enzymes did notchange. Spraying drought-stressed seedlings with JAincreased GR and Gly I activities in B. napus; increasedMDHAR activity in B. campestris; and increased DHAR,GR, GPX, Gly I and Gly II activities in B. juncea. JAimproved fresh weight, chl, RWC in all species, dry weightincreased only in B. juncea. Brassica juncea had the lowestoxidative stress under drought, indicating its naturaldrought tolerance capacity. The JA improved drought toleranceof B. juncea to the highest level among studiedspecies.

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