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      저자 : Mariscal, Marcelo M. (검색결과 5 건)
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        Blockade of cannabinoid 1 receptor improves GLP-1R mediated insulin secretion in mice

        Gonzalez-Mariscal, I.,Krzysik-Walker, S.M.,Kim, W.,Rouse, M.,Egan, J.M. North-Holland 2016 Molecular and cellular endocrinology Vol.423 No.-

        <P>The cannabinoid 1 receptor (CBI) is an important regulator of energy metabolism. Reports of in vivo and in vitro studies give conflicting results regarding its role in insulin secretion, possibly due to circulatory factors, such as incretins. We hypothesized that this receptor may be a regulator of the entero-insular axis. We found that despite lower food consumption and lower body weight postprandial GLP-1 plasma concentrations were increased in CB1(-/-) mice compared to CB1(+/+) mice administered a standard diet or high fat/sugar diet. Upon exogenous GLP-1 treatment, CB1(-/-) mice had increased glucose stimulated insulin secretion. In mouse insulinoma cells, cannabinoids reduced GLP-1R-mediated intracellular CAMP accumulation and subsequent insulin secretion. Importantly, such effects were also evident in human islets, and were prevented by pharmacologic blockade of CB1. Collectively, these findings suggest a novel mechanism in which endocannabinoids are negative modulators of incretin-mediated insulin secretion. (C) 2015 Elsevier Ireland Ltd. All rights reserved.</P>

      • KCI등재

        Anti-Helicobacter pylori Potential of Three Edible Plants Known as Quelites in Mexico

        Erika Gomez-Chang,Guadalupe Vanessa Uribe-Estanislao,Maricruz Martinez-Martinez,Amanda Galvez-Mariscal,Irma Romero 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.11

        “Quelites” are edible plants that are part of the traditional agro-ecosystems in Mexico. These plants, despite their already known nutritional properties, are now considered neglected and underutilized species. With the objective of promoting their reinsertion in the markets and mainly, in daily diets, efforts have been made to study them from multidisciplinary approaches to demonstrate their beneficial properties. To generate evidence of an added health-promoting value that would encourage quelites consumption, in the present work, the anti-Helicobacter pylori activity of three representative quelite species, Anoda cristata (Alache), Cnidoscolus aconitifolius (Chaya), and Crotalaria pumila (Chepil), was tested. H. pylori is considered the etiological agent of gastritis, ulcer, and gastric cancer, and represents a public health problem in Mexico and worldwide. Aqueous (AQ) and dichloromethane–methanol (DM) extracts were obtained from the three species of quelites to investigate their effect on H. pylori growth and on two of its colonization factors (adherence and urease activity). DM extracts from Chaya, Chepil, and Alache exert the best inhibitory effect on bacterial growth, with minimum inhibitory concentrations of 62.5, 125, and 250 μg/mL, respectively. AQ and DM extracts inhibit bacterial adhesion by 30% to 50%. None of them has an effect on urease activity. The two flavonoids present in A. cristata, acacetin and diosmetin, inhibit H. pylori growth by ∼90% with 3.9 μg/mL. These results provide new information about the anti-H. pylori potential of three edible quelites, and give an added value, since their routine consumption may impact on the prevention and/or control of H. pylori-associated diseases.

      • KCI등재

        Glycemic Index and Glycemic Load of Two Dishes Cooked with Alache (Anoda cristata) and Chaya (Cnidoscolus aconitifolius) Plants from the Traditional Mexican Diet

        Josefina Consuelo Morales-Guerrero,Reina Rosas-Romero,Ma Amanda Mariscal-Gálvez,Fabiola Ayala-Alcántara,Héctor Bourges-Rodríguez 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.6

        Correct nutrition is important for keeping good health; to attain that, the diet has to include vegetables such as quelites. The objective of this study was to determine the glycemic index (GI) and glycemic load (GL) of rice and a tamal prepared with and without two species of quelites: “alache” (Anoda cristata) and “chaya” (Cnidoscolus aconitifolius). The GI was measured in 10 healthy subjects, 7 women and 3 men, with the following mean metrics: age, 23 years old; body weight, 61.3 kg; height, 1.65 m; body mass index, 22.7 kg/m2; and basal glycemia, 77.4 mg/dL. Capillary blood samples were collected within 2 h after the meal. White rice (rice with no quelites) had a GI of 75.35 ± 15.6 and a GL of 36.17 ± 7.8; rice with alache had a GI of 33.74 ± 5.85 and a GL 33.74 ± 1.85. White tamal had a GI of 57.33 ± 10.23 and a GC of 26.65 ± 5.12; tamal with chaya had a GI of 46.73 ± 22.1 and a GL of 23.36 ± 11. The GI and GL values recorded for the combination of quelites with rice and tamal confirmed that quelites could be a good alternative for healthy diets.

      • Resveratrol Prevents β-Cell Dedifferentiation in Nonhuman Primates Given a High-Fat/High-Sugar Diet

        Fiori, Jennifer L.,Shin, Yu-Kyong,Kim, Wook,Krzysik-Walker, Susan M.,Gonzá,lez-Mariscal, Isabel,Carlson, Olga D.,Sanghvi, Mitesh,Moaddel, Ruin,Farhang, Kathleen,Gadkaree, Shekhar K.,Doyle, Maire American Diabetes Association 2013 Diabetes Vol.62 No.10

        <P>Eating a “Westernized” diet high in fat and sugar leads to weight gain and numerous health problems, including the development of type 2 diabetes mellitus (T2DM). Rodent studies have shown that resveratrol supplementation reduces blood glucose levels, preserves β-cells in islets of Langerhans, and improves insulin action. Although rodent models are helpful for understanding β-cell biology and certain aspects of T2DM pathology, they fail to reproduce the complexity of the human disease as well as that of nonhuman primates. Rhesus monkeys were fed a standard diet (SD), or a high-fat/high-sugar diet in combination with either placebo (HFS) or resveratrol (HFS+Resv) for 24 months, and pancreata were examined before overt dysglycemia occurred. Increased glucose-stimulated insulin secretion and insulin resistance occurred in both HFS and HFS+Resv diets compared with SD. Although islet size was unaffected, there was a significant decrease in β-cells and an increase in α-cells containing glucagon and glucagon-like peptide 1 with HFS diets. Islets from HFS+Resv monkeys were morphologically similar to SD. HFS diets also resulted in decreased expression of essential β-cell transcription factors forkhead box O1 (FOXO1), NKX6–1, NKX2–2, and <I>PDX1</I>, which did not occur with resveratrol supplementation. Similar changes were observed in human islets where the effects of resveratrol were mediated through Sirtuin 1. These findings have implications for the management of humans with insulin resistance, prediabetes, and diabetes.</P>

      • Ultrasmooth, Highly Spherical Monocrystalline Gold Particles for Precision Plasmonics

        Lee, You-Jin,Schade, Nicholas B.,Sun, Li,Fan, Jonathan A.,Bae, Doo Ri,Mariscal, Marcelo M.,Lee, Gaehang,Capasso, Federico,Sacanna, Stefano,Manoharan, Vinothan N.,Yi, Gi-Ra American Chemical Society 2013 ACS NANO Vol.7 No.12

        <P>Ultrasmooth, highly spherical monocrystalline gold particles were prepared by a cyclic process of slow growth followed by slow chemical etching, which selectively removes edges and vertices. The etching process effectively makes the surface tension isotropic, so that spheres are favored under quasi-static conditions. It is scalable up to particle sizes of 200 nm or more. The resulting spherical crystals display uniform scattering spectra and consistent optical coupling at small separations, even showing Fano-like resonances in small clusters. The high monodispersity of the particles we demonstrate should facilitate the self-assembly of nanoparticle clusters with uniform optical resonances, which could in turn be used to fabricate optical metafluids. Narrow size distributions are required to control not only the spectral features but also the morphology and yield of clusters in certain assembly schemes.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-12/nn404765w/production/images/medium/nn-2013-04765w_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn404765w'>ACS Electronic Supporting Info</A></P>

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