Protozoa population and carbohydrate fermentation in sheep fed diet with different plant additives

Majewska, Malgorzata P.,Miltko, Renata,Belzecki, Grzegorz,Kedzierska, Aneta,Kowalik, Barbara Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: The aim of the study was to compare the effect of two plant additives, rich in polyphenolic compounds, supplemented to sheep diets on microorganisms and carbohydrate fermentation in rumen. Methods: In the experiment, 6 ewes of the Polish Mountain breed were fitted with ruminal cannulas. Sheep were divided into three feeding groups. The study was performed in a cross-over design of two animals in each group, with three experimental periods (n = 6 per each group). The animals were fed a control diet (CON) or additionally received 3 g of dry and milled lingonberry leaves (VVI) or oak bark (QUE). Additionally, plant material was analyzed for tannins concentration. Results: Regardless of sampling time, QUE diet increased the number of total protozoa, as well as Entodinium spp., Diplodinium spp. and Isotrichidae family, while decreased bacterial mass. In turn, a reduced number of Diplodinium spp. and increased Ophryoscolex spp. population were noted in VVI fed sheep. During whole sampling time (0, 2, 4, and 8 h), the number of protozoa in ruminal fluid of QUE sheep was gradually reduced as opposed to animals receiving CON and VVI diet, where rapid shifts in the protozoa number were observed. Moreover, supplementing sheep with QUE diet increased molar proportions of butyrate and isoacids in ruminal fluid. Unfortunately, none of the tested additives affected gas production. Conclusion: The addition of VVI or QUE in a small dose to sheep diets differently affected rumen microorganisms and fermentation parameters, probably because of various contribution of catechins in tested plant materials. However, it is stated that QUE diet seems to create more favorable conditions for growth and development of ciliates. Nonetheless, the results of the present study showed that VVI and QUE additives could serve as potential natural modulators of microorganism populations and, consequently, carbohydrate digestion in ruminants.

Determination of the botanical origin of honeybee honeys based on the analysis of their selected physicochemical parameters coupled with chemometric assays

Ewa Majewska,Beata Dru_zyn´ska,Rafał Wołosiak 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.5

The aim of this paper was to study the selectphysicochemical parameters of 58 honey samples of 4different botanical origins (buckwheat, linden, rape andacacia) using multivariate methods in order to classifyhoneys according to the botanical origin. Five standardphysicochemical parameters were determined according tothe international legislation: water content, electrical conductivity,total ash content, free acidity and pH. The resultsobtained were mostly in agreement with internationalregulations. Then, the results obtained were analysed byprincipal components analysis and cluster analysis. Thechemometric analysis of results of determinations of thephysicochemical parameters demonstrated such markers aselectrical conductivity and ash content (i.e. parameterslinked with minerals content) to be the most reliablemarkers in determining the botanical origin of linden andbuckwheat honeys. Unfortunately, they appear insufficientfor reliable identification of acacia and rapeseed honeys.

Localisation of embedded water drop in glass composite using THz spectroscopy

Mieloszyk, Magdalena,Majewska, Katarzyna,Ostachowicz, Wieslaw Techno-Press 2018 Smart Structures and Systems, An International Jou Vol.21 No.6

Glass fibre reinforced polymers (GFRP) are widely exploited in many industrial branches. Due to this Structural Health Monitoring systems containing embedded fibre optics sensors are applied. One of the problems that can influence on composite element durability is water contamination that can be introduced into material structure during manufacturing. Such inclusion can be a damage origin significantly decreasing mechanical properties of an element. A non-destructive method that can be applied for inspection of an internal structure of elements is THz spectroscopy. It can be used for identifications of material discontinuities that results in changes of absorption, refractive index or scattering of propagating THz waves. The limitations of THz propagation through water makes this technique a promising solution for detection of a water inclusion. The paper presents an application of THz spectroscopy for detection and localisation of a water drop inclusion embedded in a GFRP material between two fibre optics with fibre Bragg grating sensors. The proposed filtering method allowed to determine a 3D shape of the water drop.

Growth performance, carcass and meat quality of lambs supplemented different vegetable oils

Renata Miltko,Malgorzata Paulina Majewska,Grzegorz Belzecki,Katarzyna Kula,Barbara Kowalik 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6

Objective: The purpose of this study was to evaluate the effect of rapeseed and linseed oil supplementations on performance and meat quality of lambs. Methods: The experiment was conducted on 18 growing (100-day-old) lambs of 19.7±1.9 kg live weight, assigned to 3 groups of 6 animals each. Control lambs were fed meadow hay and concentrate alone. Experimental animals additionally received rapeseed or linseed oils at a dose of 50 g/d. The lambs were slaughtered at an average body weight of 35.7±0.5 kg. Results: The dressing percentage was higher in lambs fed rapeseed oil. Total saturated fatty acids (SFA) and C15:0, C16:0, C17:0, C21:0, C24:0 were lower in longissimus dorsi muscle (MLD) in lambs fed linseed oil. Supplementation of diet with linseed oil decreased concentrations of total monounsaturated fatty acids and C16:1, C17:1, C18:1 cis-9 in MLD. The concentrations of n-3 polyunsaturated fatty acids (PUFA) and C18:3 n-3, C20:5 n-3 in MLD were higher in lambs fed linseed oil than in other groups. Oils supplementation to diets resulted in increased concentration of C22:6 n-3 in MLD. The inclusion of linseed oil into the diet increased the contents of total PUFA, n-3 PUFA and C18:3 n-3, C20:5 n-3, C22:6 n-3 in semitendinosus muscle in comparison to control. A tendency towards a lower n:6/n:3 ratio in MLD was observed when lambs were supplemented linseed oil. Conclusion: The supplementation of linseed oil to diets seems to reduce the concentration of SFA and increase the concentration of n-3 PUFA. The n-6/n-3 ratio is an important nutritional factor, and its value has been favorably decreased below 2, thereby achieving an important target related to human health. Due to these changes carcass fatty acid profile was improved, and so enhanced lamb meat healthy propertie

Growth Performance, Carcass Traits and Meat Quality of Slower-growing and Fast-growing Chickens Raised with and without Outdoor Access

Mikulski, Dariusz,Celej, Joanna,Jankowski, Jan,Majewska, Teresa,Mikulska, Marzena Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.10

The objective of this study was to determine the effect of genotype (slower-growing vs. fast-growing) and production system (access to outdoors vs. indoor) on the growth performance, carcass yield and meat quality (chemical composition, physicochemical and sensory properties) of chickens. The experiment was performed on 1,040 day-old hybrid male chickens of two genotypes. Slower-growing chickens (Hubbard JA957, certified) and fast-growing chickens (Hubbard F15) were fed identical diets until 65 days of age. Both genotypes (each represented by 520 birds) were divided into two subgroups and were raised in pens on litter with outdoor access or in indoor confinement without outdoor access (four replications per subgroup, each of 65 birds). Until day 21, the birds stayed in the indoor facility, in deep-litter pens. The birds could forage on pasture 12 h daily, commencing at three weeks of age. Stocking density was 0.13 $m^2$ floor space per bird in pens on litter, and 0.8 $m^2$ per bird in grassy yards. Compared with fast-growing, slower-growing chickens were significantly lighter (by 17%), had a lower breast and thigh muscle yield and a higher abdominal fat content, but they were characterized by higher survival rates at 65 days, a higher protein content and a lower fat content of breast meat. Outdoor access had no negative effects on the growth performance, muscle yield, the fatty acid profile and oxidative status of meat lipids. The meat of free-range chickens was darker in color, it had a higher protein content and a better water-holding capacity, but it was less juicy than the meat of birds raised indoors.