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- 저자 : LiHua Zhao (검색결과 25 건)
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Zhao, Yiping,Li, Bei,Bai, Dongyi,Huang, Jinlong,Shiraigo, Wunierfu,Yang, Lihua,Zhao, Qinan,Ren, Xiujuan,Wu, Jing,Bao, Wuyundalai,Dugarjaviin, Manglai Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.9
The hindgut of horses is an anaerobic fermentative chamber for a complex and dynamic microbial population, which plays a critical role in health and energy requirements. Research on the gut microbiota of Mongolian horses has not been reported until now as far as we know. Mongolian horse is a major local breed in China. We performed high-throughput sequencing of the 16S rRNA genes V4 hypervariable regions from gut fecal material to characterize the gut microbiota of Mongolian horses and compare them to the microbiota in Thoroughbred horses. Fourteen Mongolian and 19 Thoroughbred horses were used in the study. A total of 593,678 sequence reads were obtained from 33 samples analyzed, which were found to belong to 16 phyla and 75 genera. The bacterial community compositions were similar for the two breeds. Firmicutes (56% in Mongolian horses and 53% in Thoroughbred horses) and Bacteroidetes (33% and 32% respectively) were the most abundant and predominant phyla followed by Spirochaete, Verrucomicrobia, Proteobacteria, and Fibrobacteres. Of these 16 phyla, five (Synergistetes, Planctomycetes, Proteobacteria, TM7, and Chloroflexi) were significantly different (p<0.05) between the two breeds. At the genus level, Treponema was the most abundant genus (43% in Mongolian horses vs 29% in Thoroughbred horses), followed by Ruminococcus, Roseburia, Pseudobutyrivibrio, and Anaeroplasma, which were detected in higher distribution proportion in Mongolian horses than in Thoroughbred horses. In contrast, Oscillibacter, Fibrobacter, Methanocorpusculum, and Succinivibrio levels were lower in Mongolian horses. Among 75 genera, 30 genera were significantly different (p<0.05) between the two breeds. We found that the environment was one of very important factors that influenced horse gut microbiota. These findings provide novel information about the gut microbiota of Mongolian horses and a foundation for future investigations of gut bacterial factors that may influence the development and progression of gastrointestinal disease in horses.
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New Results on Finite-time Stabilization for Stochastic Systems with Time-varying Delay
Lihua Zhang,Wenhai Qi,Yonggui Kao,Xianwen Gao,Longjiang Zhao 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.2
The paper deals with the problem of finite-time stabilization for stochastic systems with time-varying delay by defining a new criterion for finite-time stability. Firstly, by use of more appropriate Lyapunov-Krasovskii functional (LKF), the difficulties of finite-time stability confronted in system analysis and synthesis can be overcome. Then, a state feedback controller is constructed to guarantee the closed-loop system finite-time stable. New conditions for finite-time stability analysis as well as controller synthesis are established in terms of linear matrix inequality (LMI). Finally, two practical examples demonstrate the validity of the main results.
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Zhao Chijun,Jia Xinglin,Pan Yanying,Liao Simeng,Zhang Shuo,Ji Chunxiao,Kuang Guangwei,Wu Xin,Liu Quan,Tang Yulong,Fang Lihua 한국미생물학회 2023 The journal of microbiology Vol.61 No.4
Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that can infect humans in contact with infected pigs or their byproducts. It can employ different types of genes to defend against oxidative stress and ensure its survival. The thioredoxin (Trx) system is a key antioxidant system that contributes adversity adaptation and pathogenicity. SS2 has been shown to encode putative thioredoxin genes, but the biological roles, coding sequence, and underlying mechanisms remains uncharacterized. Here, we demonstrated that SSU05_0237-ORF, from a clinical SS2 strain, ZJ081101, encodes a protein of 104 amino acids with a canonical CGPC active motif and an identity 70–85% similar to the thioredoxin A (TrxA) in other microorganisms. Recombinant TrxA efficiently catalyzed the thiol-disulfide oxidoreduction of insulin. The deletion of TrxA led to a significantly slow growth and markedly compromised tolerance of the pathogen to temperature stress, as well as impaired adhesion ability to pig intestinal epithelial cells (IPEC-J2). However, it was not involved in H2O2 and paraquat-induced oxidative stress. Compared with the wild-type strain, the ΔTrxA strain was more susceptible to killing by macrophages through increasing NO production. Treatment with TrxA mutant strain also significantly attenuated cytotoxic effects on RAW 264.7 cells by inhibiting inflammatory response and apoptosis. Knockdown of pentraxin 3 in RAW 264.7 cells was more vulnerable to phagocytic activity, and TrxA promoted SS2 survival in phagocytic cells depending on pentraxin 3 activity compared with the wild-type strain. Moreover, a co-inoculation experiment in mice revealed that TrxA mutant strain is far more easily cleared from the body than the wild type strain in the period from 8–24 h, and exhibits significantly attenuated oxidative stress and liver injury. In summary, we reveal the important role of TrxA in the pathogenesis of SS2.
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Control System Design of Anti-rolling Tank Swing Bench Using BP Neural Network PID Based on LabVIEW
Lihua Liang,Mingxiao Sun,Songtao Zhang,Yu Wen,Peng Zhao,Jia Yuan 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.6
The anti-rolling tank swing bench is a typical hydraulic position servo system. Its function is to simulate the ship roll motion and to verify the performance of the anti-rolling tank. Generally, the PID control method can achieve an ideal control effect. But the uncertainties, such as the couplings and the nonlinearty of the swing bench, make it difficult to establish the precise mathematical model of system. During experiments, the different disturbance torques are generated according to the different dimension scales of the anti-rolling tanks. Changes of the natural characteristics of the system are caused by changes of load, and the fixed parameters of PID controller are difficult to continue to ensure a good control effect. In order to reduce the influences caused by the factors mentioned above, a BP neural network PID control system based on virtual instrument LabVIEW is designed. By simulating the responses of the system in real time control, the verification tests using actual system are avoided. Finally, the experiments of swing bench are carried out. The results show that the controller can ensure the control accuracy and stability. The parameters tuning process efficiency is improved. The operation is convenient. The data acquisition process is simple and practical.
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Stochastic Stability, ℒ1-gain and Control Synthesis for Positive Semi-Markov Jump Systems
Longjiang Zhao,Wenhai Qi,Lihua Zhang,Yonggui Kao,Xianwen Gao 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.5
This paper treats the problems of stochastic stability, ℒ1-gain and control synthesis for positive semi- Markov jump systems (S-MJSs). The system under consideration involves semi-Markov stochastic process related to Weibull distribution. The main motivation for this paper is that the positive condition sometimes needs to be considered in S-MJSs and the controller design methods in the existing works have some conservation. To deal with these problems, some sufficient conditions for stochastic stability of positive S-MJSs are established by implying the linear co-positive Lyapunov function. Then, some sufficient conditions for ℒ1-gain constraint are also presented, upon which, a state feedback controller is designed by decomposing the controller gain matrix such that the resulting closed-loop system is positive and stochastically stable with ℒ1-gain performance in the form of standard linear programming (LP). The advantages of the new framework lie in the following facts: (1) the weak infinitesimal operator is derived for S-MJSs under the constraint of positive condition and (2) the less conservative stabilizing controller is designed to achieve the desired control performance. Finally, numerical examples are given to demonstrate the validity of the main results.
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Qiu, Jijun,Weng, Binbin,Zhao, Lihua,Chang, Caleb,Shi, Zhisheng,Li, Xiaomin,Kim, Hyung-Kook,Hwang, Yoon-Hwae Hindawi Limited 2014 Journal of nanomaterials Vol.2014 No.-
<P>Flower-like bundles of ZnO nanosheets have been prepared by using preheating hydrothermal process without any surfactants. The flower-like bundles consist of many thin and uniform hexagonal-structured ZnO nanosheets, with a thickness of 50 nm. The selected area electronic diffraction (SAED) and high-resolution transmission electron microscope (HRTEM) images indicate that the ZnO nanosheets are single crystal in nature. The growth mechanism of the flower-like bundles of ZnO nanosheets is discussed based on the morphology evolution with growth times and reaction conditions. It is believed that the formation of flower-like bundles of ZnO nanosheets is related to the shielding effect of OH<SUP>−</SUP>ions and the self-assembly process, which is dominated by a preheating time. Room temperature photoluminescence spectra results show that the annealing atmosphere strongly affects the visible emission band, which is sensitive to intrinsic and surface defects, especially oxygen interstitials, in flower-like bundles of ZnO nanosheets.</P>
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Yaoyu Wang,Linan Gu,Lihua Zhao 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11
This paper describes research into the effectiveness of using various nanomaterials, admixtures, and fibers as concrete additives to increase the compressive strength of non-dispersible underwater concrete (NUC). The suitable content of nanomaterials, admixtures, and fibers was determined based on slump tests, cement mass loss, and compressive strength tests for specimens. X–ray diffraction (XRD), mercury intrusion porosimetry (MIP), and scanning electron microscopy (SEM) were used to identify the crystalline composition and microstructure characteristics of the modified NUCs. The results showed that the addition of nano-SiO2 or nanometakaolin significantly increased the compressive strength of the NUC. The compressive strength of the NUC containing 3% nano-SiO2 or 5% nano-metakaolin increased by 33.5% or 26.4% at 3 days, respectively, and by 52.1% or 32.6% at 28 days, respectively. XRD, MIP, and SEM showed that the nano-SiO2 and nanometakaolin filled internal pores and initiated a pozzolanic effect, making the NUC more complex and increasing its density. The nanomaterials altered the internal structure of the NUC and thus increased its compressive strength. The recommended additives to NUC will make NUC better able to resist a damaging seawater environment, and they have usage potential for real projects.
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Effects of Submicron Metakaolin on Hydration and Compressive Strength of Portland Cement Slurry
Yao Yu Wang,Lihua Zhao,Jing Zhao 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7
Submicron metakaolin (SMK) is an ultrafine aluminosilicate material, which was used for improving properties of cement slurry in this paper. SMK was added to the slurry mix as a partial substitute for Portland cement in proportions of 1%, 3%, 5% and 7%. To explore the influence of SMK on hydration of cement slurry, the heat flow and pH measurement was used to indicate that SMK significantly increased the rate of heat release and total heat release,increase the rate of calcium hydroxide (CH) generation in the pore solution at initial stage of hydration. The hardened specimens at different ages (3 – 28 days) were measured by X–ray diffraction, Differential Thermal Analysis and Compressive strength. The results showed that:SMK displayed pozzolanic effect which reduced CH content and increased the generation of Calcium Silicate Hydrate (C-S-H) gel at later stages of hydration. The compressive strength tests showed that SMK highest increased compressive strength of the slurry by 67% and 19% at 3d and 28d over plain cement slurry. It was concluded that SMK could improve manyproperties of cement slurry and has the use potential on actual projects.
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