Colorectal Cancer Concealment Predicts a Poor Survival: A Retrospective Study

Li, Xiao-Pan,Xie, Zhen-Yu,Fu, Yi-Fei,Yang, Chen,Hao, Li-Peng,Yang, Li-Ming,Zhang, Mei-Yu,Li, Xiao-Li,Feng, Li-Li,Yan, Bei,Sun, Qiao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Objectives: Understanding the situation of cancer awareness which doctors give to patients might lead to prognostic prediction in cases of of colorectal cancer (CRC). Methods: Subsets of 10,779 CRC patients were used to screen the risk factors from the Cancer Registry in Pudong New Area in cancer awareness, age, TNM stage, and gender. Survival of the patients was calculated by the Kaplan-Meier method and assessed by Cox regression analysis. The views of cancer awareness in doctors and patients were surveyed by telephone or household. Results: After a median observation time of 1,616 days (ranging from 0 to 4,083 days) of 10,779 available patients, 2,596 of the 4,561 patients with cancer awareness survived, whereas 2,258 of the 5,469 patients without cancer awareness and 406 of the 749 patients without information on cancer awareness died of the disease. All-cause and cancer-specific survival were poorer for the patients without cancer awareness than those with (P < 0.001 for each, log-rank test). Cox multivariate regression analysis showed that cancer concealment cases had significantly lower cancer-specific survival (hazard ratio (HR) = 1.299; 95 % confidence interval (CI): 1.200-1.407)and all-cause survival (HR = 1.324; 95 % CI: 1.227-1.428). Furthermore, attitudes of cancer awareness between doctors and patients were significantly different (P < 0.001). Conclusion: Cancer concealment, not only late-stage tumor and age, is associated with a poor survival of CRC patients.

SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin–Rho GTPase–Hippo Pathways

Zhang, Yan-Li,Li, Qing,Yang, Xiao-Mei,Fang, Fang,Li, Jun,Wang, Ya-Hui,Yang, Qin,Zhu, Lei,Nie, Hui-Zhen,Zhang, Xue-Li,Feng, Ming-Xuan,Jiang, Shu-Heng,Tian, Guang-Ang,Hu, Li-Peng,Lee, Ho-Young,Lee, Su-J American Association for Cancer Research 2018 Cancer research Vol.78 No.9

<P>Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P>Tumor-associated macrophages (TAM) represent key regulators of the complex interplay between cancer and the immune microenvironment. Matricellular protein SPON2 is essential for recruiting lymphocytes and initiating immune responses. Recent studies have shown that SPON2 has complicated roles in cell migration and tumor progression. Here we report that, in the tumor microenvironment of hepatocellular carcinoma (HCC), SPON2 not only promotes infiltration of M1-like macrophages but also inhibits tumor metastasis. SPON2-α4β1 integrin signaling activated RhoA and Rac1, increased F-actin reorganization, and promoted M1-like macrophage recruitment. F-Actin accumulation also activated the Hippo pathway by suppressing LATS1 phosphorylation, promoting YAP nuclear translocation, and initiating downstream gene expression. However, SPON2-α5β1 integrin signaling inactivated RhoA and prevented F-actin assembly, thereby inhibiting HCC cell migration; the Hippo pathway was not noticeably involved in SPON2-mediated HCC cell migration. In HCC patients, SPON2 levels correlated positively with prognosis. Overall, our findings provide evidence that SPON2 is a critical factor in mediating the immune response against tumor cell growth and migration in HCC.</P><P><B>Significance:</B> Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P><B>Graphical Abstract:</B> http://cancerres.aacrjournals.org/content/canres/78/9/2305/F1.large.jpg. <I>Cancer Res; 78(9); 2305–17. ©2018 AACR</I>.</P><P><B>Graphical Abstract</B></P><P> [Figure]</P>

Knockdown of HMGN5 Expression by RNA Interference Induces Cell Cycle Arrest in Human Lung Cancer Cells

Chen, Peng,Wang, Xiu-Li,Ma, Zhong-Sen,Xu, Zhong,Jia, Bo,Ren, Jin,Hu, Yu-Xin,Zhang, Qing-Hua,Ma, Tian-Gang,Yan, Bing-Di,Yan, Qing-Zhu,Li, Yan-Lei,Li, Zhen,Yu, Jin-Yan,Gao, Rong,Fan, Na,Li, Bo,Yang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

HMGN5 is a typical member of the HMGN (high mobility group nucleosome-binding protein) family which may function as a nucleosomal binding and transcriptional activating protein. Overexpression of HMGN5 has been observed in several human tumors but its role in tumorigenesis has not been fully clarified. To investigate its significance for human lung cancer progression, we successfully constructed a shRNA expression lentiviral vector in which sense and antisense sequences targeting the human HMGN5 were linked with a 9-nucleotide loop. Inhibitory effects of siRNA on endogenous HMGN5 gene expression and protein synthesis were demonstrated via real-time RT-PCR and western blotting. We found HMGN5 silencing to significantly inhibit A549 and H1299 cell proliferation assessed by MTT, BrdU incorporation and colony formation assays. Furthermore, flow cytometry analysis showed that specific knockdown of HMGN5 slowed down the cell cycle at the G0/G1 phase and decreased the populations of A549 and H1299 cells at the S and G2/M phases. Taken together, these results suggest that HMGN5 is directly involved in regulation cell proliferation in A549 and H1299 cells by influencing signaling pathways involved in cell cycle progression. Thus, our finding suggests that targeting HMGN5 may be an effective strategy for human lung cancer treatment.

Competitive neutrophil elastase inhibitory isoflavones from the roots of <i>Flemingia philippinensis</i>

Kim, Jeong Yoon,Wang, Yan,Uddin, Zia,Song, Yeong Hun,Li, Zuo Peng,Jenis, Janar,Park, Ki Hun Elsevier 2018 Bioorganic chemistry Vol.78 No.-

<P><B>Abstract</B></P> <P> <I>Flemingia philippinensis</I> has been used throughout history to cure rheumatism associated with neutrophil elastase (NE). In this study, we isolated sixteen NE inhibitory flavonoids (<B>1</B>–<B>16</B>), including the most potent and abundant prenyl isoflavones (<B>1</B>–<B>9</B>), from the <I>F. philippinensis</I> plant. These prenyl isoflavones (<B>2</B>, <B>3</B>, <B>5</B>, <B>7</B>, and <B>9</B>) competitively inhibited NE, with IC<SUB>50</SUB> values of 1.3–12.0 μM. In addition, they were reversible, simple, slow-binding inhibitors according to their respective parameters. Representative compound <B>3</B> had an IC<SUB>50</SUB> = 1.3 μM, <I>k</I> <SUB>3</SUB> = 0.04172 μM<SUP>−1</SUP> min<SUP>−1</SUP>, <I>k</I> <SUB>4</SUB> = 0.0064 min<SUP>−1</SUP>, and <I>K</I> <SUB>i</SUB> <SUP>app</SUP> = 0.1534 μM. The <I>K</I> <SUB>ik</SUB>/<I>K</I> <SUB>iv</SUB> ratios (18.5 ∼ 24.6) for compound <B>3</B> were consistent with typical competitive inhibitors. The prenyl functionality of isoflavones significantly affected inhibitory potencies and mechanistic behavior by shifting the competitive mode to a noncompetitive one. The remaining flavonoids (<B>10</B>–<B>16</B>) were confirmed as mixed type I inhibitors that preferred to bind free enzyme rather than the enzyme-substrate complex. Fluorescence quenching analyses indicated that the inhibitory potency (IC<SUB>50</SUB>) closely followed the binding affinity (<I>K</I> <SUB>SV</SUB>).</P> <P><B>Highlights</B></P> <P> <UL> <LI> Phenolic isolates from <I>Flemingia philippinensis</I>. </LI> <LI> <I>In vitro</I> neutrophil elastase inhibitory activity. </LI> <LI> Characterization of kinetics based on structure. </LI> <LI> Binding affinity between inhibitor and enzyme. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Zeolite-catalyzed Isomerization of 1-Hexene to trans-2-Hexene: An ONIOM Study

Li, Yan-Feng,Zhu, Ji-Qin,Liu, Hui,He, Peng,Wang, Peng,Tian, Hui-Ping Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.6

Details of the double-bond isomerization of 1-hexene over H-ZSM-5 were clarified using density functional theory. It is found that the reaction proceeds by a mechanism which involves the Br${\o}$nsted acid part of the zeolite solely. According to this mechanism, 1-hexene is first physically adsorbed on the acidic site, and then, the acidic proton transfers to one carbon atom of the double bond, while the other carbon atom of the double bond bonds with the Br${\o}$nsted host oxygen, yielding a stable alkoxy intermediate. Thereafter, the Br${\o}$nsted host oxygen abstracts a hydrogen atom from the $C_6H_{13}$ fragment and the C-O bond is broken, restoring the acidic site and yielding trans-2-hexene. The calculated activation barrier is 12.65 kcal/mol, which is in good agreement with the experimental value. These results well explain the energetic aspects during the course of double-bond isomerization and extend the understanding of the nature of the zeolite active sites.

Effect of stacking conditions on performance of a centrifugal pump

Peng Yan,Shiyang Li,Shuai Yang,Peng Wu,Dazhuan Wu 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.2

In this study, the effect of stacking condition of centrifugal pump impeller was investigated by Computational fluid dynamics (CFD). The performance curves of impellers with three different stacking conditions were compared. The Local Euler head distributions (LEH distribution) at blade Leading edge (LE) and Trailing edge (TE) were obtained to evaluate the contribution of different spanwise location to the total work. The positive stacking characterised by the hub leading the shroud was considered inappropriate in centrifugal pump impellers as it deteriorated the performance of the pump in whole flow rate range. The negative stacking characterised by the shroud leading the hub could improve flow stability and efficiency in low flow rates, it was manufactured and tested. The test results showed good agreement with CFD calculations. A hump zone was observed in zero stacking impeller between 0.9Q d and Q d . It was closely related to the drastic change in pattern of LEH distribution at the two flow rates. The internal flow was analyzed to reveal the reasons responsible for the hump in head curve. It is found that the tremendous extension of low streamwise velocity region and its instant movement from the corner of shroud and blade suction surface to the midspan near blade suction surface when the flow rate decreases from Q dto 0.9Q d is the main reason for flow instability in zero stacking impeller.

Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening

Yan Fu,Jingchao Lan,Xuhang Wu,Deying Yang,Zhihe Zhang,Huaming Nie,Rong Hou,Runhui Zhang,Wanpeng Zheng,Yue Xie,Ning Yan,Zhi Yang,Chengdong Wang,Li Luo,Li Liu,Xiaobin Gu,Shuxian Wang,Xuerong Peng,Guangy 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.1

H-형태 양친매성 펜타블록 공중합체의 화학효소적 합성과 자기회합거동 평가

Peng Chen,Ya Peng Li,Can Jin Li,Xin Lei Meng,Bao Zhang,Ming Zhu,Yan-jing Liu,Jing Yuan Wang 한국고분자학회 2013 폴리머 Vol.37 No.3

H-shaped amphiphilic pentablock copolymers (PSt)2-b-PCL-b-PEO-b-PCL-b-(PSt)2 was synthesized via chemoenzymatic method by combining enzyme-catalyzed ring-opening polymerization (eROP) of ε-caprolactone (ε-CL) and atom transfer radical polymerization (ATRP) of styrene. By this process, we obtained copolymers with controlled molecular weight and low polydispersity. The structure and composition of the obtained copolymers were characterized by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC) and infrared spectroscopy analysis (IR). The crystallization behavior of the copolymers was analyzed by differential scanning calorimetry (DSC) and X-ray diffraction (XRD). The crystallization behavior of the H-shaped block copolymers demonstrated a PCL dominate crystallization. The self-assembly behavior of the copolymers was investigated in aqueous media. The hydrodynamic diameters of the copolymer micelles in aqueous solution were measured by dynamic light scattering (DLS). The morphology of the copolymer micelles was observed by atomic force microscopy (AFM) and transmission electron microscopy (TEM). The hydrodynamic diameters of spherical micelles declined gradually with the increase of the hydrophobic chain lengths of the copolymers. The critical micelle concentration (CMC) values were determined from fluorescence emission, and it was found that the CMCs decreased with an increase of PSt hydrophobic block lengths.

Analysis of the Relationship between MHC-DRB1 Gene Polymorphism and Hydatidosis in Kazakh Sheep

Li, Ren-Yan,Jia, Bin,Zhang, Wen-Ju,Zhao, Zong-Sheng,Shi, Guo-Qing,Shen, Hong,Peng, Qiang,Lv, Li-Min,Zhou, Qi-Wei,Du, Ying-Chun Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9

The objective of this work was to analyze the relationship between ovine major histocompatibility complex (MHC) DRB1 gene polymorphism and genetic resistance to hydatidosis in Kazakh sheep. The Ovar (ovine MHC) class II DRB1 second exon was amplified by polymerase chain reaction (PCR) from DNA samples of 702 Kazakh sheep, including 302 sheep with hydatidosis and 400 health controls. PCR products were characterized by the restriction fragment length polymorphism (RFLP) technique using five restriction enzymes, i.e., MvaI, HaeIII, SacI, SacII and Hin1I, yielding 14 alleles and 28 genotypes. Comparing the frequency of genotypes in hydatidosis sheep with the control group, it was found that the genotype frequencies of MvaIbc, Hin1Iab, SacIIab, HaeIIIde, HaeIIIdf and HaeIIIdd in control sheep were significantly (p<0.01) higher than in hydatidosis sheep, indicating that a significant correlation existed between these genotypes and resistance to hydatidosis. Genotype frequencies of MvaIbb, SacIIaa, Hin1Ibb and HaeIIIef in sheep with hydatidosis were extremely significantly (p<0.01) higher than in the control group, and the genotype frequency of HaeIIIab was significantly higher (p<0.05), indicating that a marked correlation existed between these genotypes and susceptibility to hydatidosis. By way of analyzing haplotype with these resistant genotypes, the hydatidosis resistant haplotype MvaIbc-SacIIab-Hin1Iab of Kazakh sheep was screened out, and then verified through artificial hydatid infection in sheep. The results indicated that the infection rate of sheep with the resistant haplotype of hydatidosis was significantly lower (p<0.01) than without this resistant haplotype. It showed that the genic haplotype MvaIbc-SacIIab-Hin1Iab of Ovar-DRB1 exon 2 was the resistant haplotype of hydatidosis in Kazakh sheep.

Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid

Yan Lu,Jia-Hui Sun,Li-Li Lu,Jia-Xu Chen,Peng Song,Lin Ai,Yu-Chun Cai,Lan-Hua Li,Shao-Hong Chen 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.6