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( Bo-kyoung Jung ),( Hye-ran Kim ),( Young-hyeon Lee ),( Hyun Jang ),( Kyung-soo Chang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. Replicase (Rep) proteins are considered essential for viral replication. Capsid (Cap) protein is the primary immunogenic protein that induces protective immunity. Little is known about comparison on the immunogenicity of PCV2 Rep and Cap fusion protein and Cap protein. In the present study, recombinant baculoviruses expressing the Rep-Cap fusion protein (Bac-Rep-Cap) and the Cap protein (Bac-Cap) of PCV2 were constructed and confirmed with western blot and indirect fluorescence assay. Immunogenicities of the two recombinant proteins were tested in mice. The titers of antibodies were determined with a PCV2-specific enzyme-linked immunosorbent assay (ELISA) and a serum neutralization assay. The IFN-γ response of immunized mice was measured by ELISA. The mice immunized with the Bac-Rep-Cap and Bac-Cap successfully produced Cap-specific immunoreaction. The mice immunized with the Bac-Cap developed higher PCV2-specific neutralizing antibody titers than mice injected with the Bac-Rep-Cap. IFN-γ in the Bac-Rep-Cap group was increased compared to those in the Bac-Cap group. Vaccination of mice with the Bac-Rep-Cap showed significantly decreased protective efficacy compared to the Bac-Cap. Our findings will indubitably not only lead to a better understanding of the immunogenicity of PCV2, but also improved vaccines.
Congenital Cutaneous Candidiasis in a Healthy Term Baby: A Case of Report
( Bo Yoon Seo ),( Tae Woong Um ),( Hae Sun Park ),( Ran Lee ),( Hye Won Park ) 대한주산의학회 2021 Perinatology Vol.32 No.3
Congenital Candida infection is very rare in newborns and its findings vary from skin involvement only to systemic involvement with respiratory distress and sepsis. Our patient was an otherwise healthy, full-term infant who developed a whole-body rash within 7 hours after birth. The patient’s general condition was good, but the skin rash persisted without response to antibiotics. Candida was detected on a potassium hydroxide (KOH) smear. After the diagnosis of congenital cutaneous candidiasis, the patient was treated with intravenous fluconazole and improved rapidly. The erythematous papules and diaper rash disappeared completely by day 7. Treatment was completed without recurrence or complications after discontinuing the fluconazole. We report on the diagnosis and treatment of congenital cutaneous candidiasis in a healthy term infant.
Lee, Mi-Ni,Lee, Shi-Nai,Kim, Se-Hee,Kim, Bora,Jung, Bo-Kyung,Seo, Ji Hae,Park, Ji-Hyeon,Choi, Jae-Hoon,Yim, Sun Hee,Lee, Mi-Ran,Park, Jong-Gil,Yoo, Ji-Young,Kim, Jeong Hun,Lee, Seung-Taek,Kim, Hwan-Mo Oxford University Press 2010 Journal of the National Cancer Institute Vol.102 No.6
<P><B>Background</B></P><P>Vascular endothelial growth factor A (VEGFA), a critical mediator of tumor angiogenesis, is a well-characterized target of hypoxia-inducible factor 1 (HIF-1). Murine arrest-defective protein 1A (mARD1A<SUP>225</SUP>) acetylates HIF-1α, triggering its degradation, and thus may play a role in decreased expression of VEGFA.</P><P><B>Methods</B></P><P>We generated Apc<SUP>Min/+</SUP>/mARD1A<SUP>225</SUP> transgenic mice and quantified growth of intestinal polyps. Human gastric MKN74 and murine melanoma B16F10 cells overexpressing mARD1A<SUP>225</SUP> were injected into mice, and tumor growth and metastasis were measured. VEGFA expression and microvessel density in tumors were assessed using immunohistochemistry. To evaluate the role of mARD1A<SUP>225</SUP> acetylation of Lys532 in HIF-1α, we injected B16F10-mARD1A<SUP>225</SUP> cell lines stably expressing mutant HIF-1α/K532R into mice and measured metastasis. All statistical tests were two-sided, and <I>P</I> values less than .05 were considered statistically significant.</P><P><B>Results</B></P><P>Apc<SUP>Min/+</SUP>/mARD1A<SUP>225</SUP> transgenic mice (n = 25) had statistically significantly fewer intestinal polyps than Apc<SUP>Min/+</SUP> mice (n = 21) (number of intestinal polyps per mouse: Apc<SUP>Min/+</SUP> mice vs Apc<SUP>Min/+</SUP>/mARD1A<SUP>225</SUP> transgenic mice, mean = 83.4 vs 38.0 polyps, difference = 45.4 polyps, 95% confidence interval [CI] = 41.8 to 48.6; <I>P</I> < .001). The growth and metastases of transplanted tumors were also statistically significantly reduced in mice injected with mARD1A<SUP>225</SUP>-overexpressing cells than in mice injected with control cells (<I>P</I> < .01). Moreover, overexpression of mARD1A<SUP>225</SUP> decreased VEGFA expression and microvessel density in tumor xenografts (<I>P</I> < .04) and Apc<SUP>Min/+</SUP> intestinal polyps (<I>P</I> = .001). Mutation of lysine 532 of HIF-1α in B16F10-mARD1A<SUP>225</SUP> cells prevented HIF-1α degradation and inhibited the antimetastatic effect of mARD1A<SUP>225</SUP> (<I>P</I> < .001).</P><P><B>Conclusion</B></P><P>mARD1A<SUP>225</SUP> may be a novel upstream target that blocks VEGFA expression and tumor-related angiogenesis.</P>
First Observation of Ferroelectricity in ∼1 nm Ultrathin Semiconducting BaTiO<sub>3</sub> Films
Lee, Seung Ran,Baasandorj, Lkhagvasuren,Chang, Jung Won,Hwang, In Woong,Kim, Jeong Rae,Kim, Jeong-Gyu,Ko, Kyung-Tae,Shim, Seung Bo,Choi, Min Woo,You, Mujin,Yang, Chan-Ho,Kim, Jinhee,Song, Jonghyun American Chemical Society 2019 NANO LETTERS Vol.19 No.4
<P>The requirements of multifunctionality in thin-film systems have led to the discovery of unique physical properties and degrees of freedom, which exist only in film forms. With progress in growth techniques, one can decrease the film thickness to the scale of a few nanometers (∼nm), where its unique physical properties are still pronounced. Among advanced ultrathin film systems, ferroelectrics have generated tremendous interest. As a prototype ferroelectric, the electrical properties of BaTiO<SUB>3</SUB> (BTO) films have been extensively studied, and it has been theoretically predicted that ferroelectricity sustains down to ∼nm thick films. However, efforts toward determining the minimum thickness for ferroelectric films have been hindered by practical issues surrounding large leakage currents. In this study, we used ∼nm thick BTO films, exhibiting <I>semiconducting</I> characteristics, grown on a LaAlO<SUB>3</SUB>/SrTiO<SUB>3</SUB> (LAO/STO) heterostructure. In particular, we utilized two-dimensional electron gas at the LAO/STO heterointerface as the bottom electrode in these capacitor junctions. We demonstrate that the BTO film exhibits ferroelectricity at room temperature, even when it is only ∼2 unit-cells thick, and the total thickness of the capacitor junction can be reduced to less than ∼4 nm. Observation of ferroelectricity in ultrathin <I>semiconducting</I> films and the resulting shrunken capacitor thickness will expand the applicability of ferroelectrics in the next generation of functional devices.</P> [FIG OMISSION]</BR>
Ran, Weiguang,Noh, Hyeon Mi,Park, Sung Heum,Lee, Bo Ram,Kim, Jung Hwan,Jeong, Jung Hyun,Shi, Jinsheng Elsevier 2019 Materials research bulletin Vol.117 No.-
<P><B>Abstract</B></P> <P>Er<SUP>3+</SUP>-activated CdMoO<SUB>4</SUB> phosphors were prepared by a traditional high-temperature solid-state method. The crystal structure, photoluminescence (PL) characteristics and performance in non-contact thermometry of as-prepared phosphors were studied in detail. Benefiting from the 3D network structure of CdMoO<SUB>4</SUB> host, the desirable green emission of Er<SUP>3+</SUP>-based phosphors were observed. Especially, based on the thermally coupled energy levels, the obtained phosphors show enhanced emission intensity in high temperatures. When applied to the temperature sensor based on the fluorescence intensity ratio (FIR), the prepared CdMoO<SUB>4</SUB>:0.02Er<SUP>3+</SUP> phosphor show an excellent sensitivity (0.875% K<SUP>−1</SUP>) at 483 K. Therefore, it is demonstrated that the as-prepared Er<SUP>3+</SUP>-activated CdMoO<SUB>4</SUB> phosphors have a promising potential application in both solid-state lighting and non-contact thermometry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced emission intensity in high temperatures has been achieved. </LI> <LI> Er<SUP>3+</SUP> doped CdMoO<SUB>4</SUB> phosphor has a potential application in solid-state lighting. </LI> <LI> High sensitivity indicates that CdMoO<SUB>4</SUB>: 0.02Er<SUP>3+</SUP> phosphor can be used in non-contact thermometer. </LI> <LI> Excellent water stability of Er<SUP>3+</SUP> doped CdMoO<SUB>4</SUB> can be used in a complex condition. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
( Bo Ra Lee ),( Yu Mi Ko ),( Mi Hee Cho ),( Young Ran Yoon ),( Seung Hee Kye ),( Yoo Kyoung Park ) 한국임상영양학회 2016 Clinical Nutrition Research Vol.5 No.2
This study investigated the effect of switching normal diet to vegetarian diet rich in vegetables and fruits for school foodservice and home meal on the nutritional status, bowel habit improvement and stress reduction of teachers and adolescents. A total of 40 research subjects (26 students, 14 teachers) from one middle school voluntarily participated in the research. Questionnaire surveys and blood analysis were conducted before and after a 12-week vegetarian diet period. The participants were asked on their dietary habit, bowel habit and stress measurement. After 12 weeks, reduction of BMI (kg/m²) in the students (p < 0.05) and reduction of TC (mg/dL) in both teachers and students (p < 0.05) were observed. Also reduction of LDL-C (mg/dL) was observed in the teachers (p < 0.05) whereas serum calcium and Vitamin B12 was increased in the students and teachers (p < 0.005). The teacher`s stress level was reduced (p < 0.05) after the 12-week vegetarian diet. As for the changes in bowel habit, the number of the students and teachers classified as experiencing functional constipation was decreased respectively from 10 to 7, from 7 to 5. Based on the result, it is considered that the vegetarian diet rich in fruits and vegetables improved general health status of study subjects suggesting that such a dietary habit would substantially contribute to improving nutritional status and bowel habit.
Pharmacokinetic and safety evaluation of MB12066, an NQO1 substrate
Lee, Hae Won,Seong, Sook Jin,Ohk, Boram,Kang, Woo Youl,Gwon, Mi-Ri,Kim, Bo Kyung,Kim, Hyun-Ju,Yoon, Young-Ran Dove Medical Press 2017 Drug design, development and therapy Vol.11 No.-
<P><B>Objective</B></P><P>This study evaluated the pharmacokinetics (PKs) and safety of a newly developed β-lapachone (MB12066) tablet, a natural NAD(P)H:quinone oxidoreductase 1 (NQO1) substrate, in healthy male volunteers.</P><P><B>Methods</B></P><P>In a randomized, double-blind, multiple-dose, two-treatment study, 100 mg MB12066 or placebo was given twice daily for 8 days to groups of eight or three fasted healthy male subjects, respectively, followed by serial blood sampling. Plasma concentrations for β-lapachone were determined using liquid chromatography–tandem mass spectrometry. PK parameters were obtained with non-compartmental analysis. Tolerability was assessed based on physical examinations, vital signs, clinical laboratory tests, and electrocardiograms.</P><P><B>Results</B></P><P>Following a single 100 mg MB12066 oral dose, maximum plasma concentration (<I>C</I><SUB>max</SUB>) of β-lapachone was 3.56±1.55 ng/mL, and the median (range) time to reach <I>C</I><SUB>max</SUB> was 3 h (2–5 h). After the 8 days of 100 mg twice daily repeated dosing was completed, mean terminal half-life was determined to be 18.16±3.14 h, and the mean area under the plasma concentration vs time curve at steady state was 50.44±29.68 ng·h/mL. Accumulation index was 2.72±0.37. No serious adverse events (AEs) were reported, and all reported intensities of AEs were mild.</P><P><B>Conclusion</B></P><P>The results demonstrated that MB12066 was safe and well tolerated in healthy volunteers and that there were no serious AEs. Accumulation in plasma with twice-daily administration was associated with a 2.72 accumulation ratio.</P>