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Laila Gasmi,Sehyeon Baek,Jong Cheol Kim,Mi Rong Lee,Jae Su Kim 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Beauveria comprises entomopathogenic fungi frequently isolated from insect cadavers, among which Beauveria bassiana is the most widely studied species of entomopathogenic fungal genus for its high potential as a biological pesticide. Even though it has been reported that B. bassiana is a heterogeneous assemblage of strains, little is known about the factors that might drive the genetic diversity among various isolates. In this work, we ought to study the gene diversity of 33 isolates in order to figure out the relationship between their gene diversity and biological features. First, we analyzed gene sequences that are involved in developmental and immunological processes of the fungal isolates and host. Now we are checking whether the gene diversity is related to the genes function, the isolates geographic localization, thermo-tolerance and virulence of the isolates. The obtained results so far showed no correlation between the genetic diversity and the fungal geographic localization. But interestingly, a positive correlation has been obtained between the virulence and the genetic diversity based on the internal transcribed spacers (ITS). Further analyses are aiming to confirm the obtained data and to analyze possible correlations between the genetic diversity and morphological and transcriptional data of the studied isolates. Based on the obtained results, we could much strongly focus on interesting genes which might be related to improving or screening highly virulent fungal agents as biological control agents.
Gene diversity shapes biological features of entomopathogenic Beauveria bassiana
Laila Gasmi,Sehyeon Baek,Jong Cheol Kim,Mi Rong Lee,Sihyeon Kim,So Eun Park,Dongwei Li,Tae Young Shin,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Beauveria bassiana is widely studied for its potential as biopesticide. However, little is known about the factors to influence genetic diversity among isolates. In this work, we aimed to study the gene diversity of 42 isolates to figure out the impact of sequences variability on various biological features. The accumulated data showed no correlation between sequence variability and the fungal geographic localization. Meanwhile, weak to strong correlations have been obtained between the sequence diversity of various genes and the studied biological features. Interestingly, Biotrophy-associated gene 2 gene variability was correlated with all the studied biological features, being an important marker to determine isolates of interest for biological control. Therefore, the obtained data would serve as a database to focus on interesting genes related to improving or screening highly virulent isolates as biological control agents.
Sihyeon Kim,Jong Cheol Kim,Mi Rong Lee,So Eun Park,Dongwei Li,Sehyeon Baek,Laila Gasmi,Min Sung Jo,Tae Young Shin,Junheon Kim,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Insect killing fungus Beauveria bassiana has been widely studied as a biological control agent. However, many studies have been focused on lab or field-based management. Herein this work, comparison of three B. bassiana strains was investigated under a molecular level. The whole genome sequences of ERL836, JEF-007 were analyzed by PacBio (35.5 Mb of ERL836 and 36.5 Mb of JEF-007) and ARSEF2860 referenced from GenBank (33.7 Mb). To compare the three strains, virulence, thermotolerance and chemical resistance were assayed. The transcriptomes of non-infecting B. bassiana and infecting B. bassiana against western flower thrips were analyzed using RNA-seq. This work can provide that genome features, functions, morphology and gene expression could be different under the molecular level, even if in the same species.
Insecticidal activity of entomopathogenic fungal species against silverleaf whitefly, Bemisia tabaci
Sehyeon Baek,Sihyeon Kim,Jong Cheol Kim,Mi Rong Lee,So Eun Park,Dongwei Li,Laila Gasmi,Tae Young Shin,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Silverleaf whitefly, Bemisia tabaci is a worldwide agricultural pest that cause serious damage to crops. However, since this insect developed resistance to variety of chemicals, alternative control strategy needs to be studied. In this study, we aimed to evaluate the potential of entomopathogenic fungi as biological control tools against this pest. We developed a dipping method in laboratory conditions to screen insecticidal activity of different fungal species and isolates against the nymphal stage of whitefly. The highly virulent isolates were characterized and 2 isolates were further selected based on their high conidial productivity and thermotolerance. Finally, several grain substrates were used to confirm the conidial productivity and thermotolerance of the selected isolates, revealing optimum conidial production when the fungi were cultured on millet grains. Both isolates could be used in further millet grain-based formulations to control the whitefly in fields.
dsRNA-based gene functional study in insect killing Beauveria bassiana
Tae Young Shin,Mi Rong Lee,Se Jin Lee,Jong Cheol Kim,Sihyeon Kim,So Eun Park,Dongwei Li,Sehyeon Baek,Laila Gasmi,Jae Su Kim 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
A species of Beauveria bassiana is widely used for biological pest management in many countries. Many efforts have been given to figure out the clear fungal mode of action to enhance the insecticidal activity. Homologous recombination (knock-out) or hairpin RNA (knock-down) is popularly used in fungal gene function study, but gene cloning and generation of knock-out or -down mutants takes long time or temporarily knock-downed. Here in this work, we used previously generated egfp-expressing B. bassiana strain (Bb-egfp #3) and integrated dsegfp to the Bb-egfp #3 using a protoplast integration method. This work suggests that protoplast integration with dsRNA possibly generate significantly reduced gene expression in B. bassiana and the reduction is quite stable over generations which provide easy of functional study for fungal mode of action.
( Sihyeon Kim ),( Jong Cheol Kim ),( Se Jin Lee ),( Mi Rong Lee ),( So Eun Park ),( Dongwei Li ),( Sehyeon Baek ),( Tae Young Shin ),( Laila Gasmi ),( Jae Su Kim ) 한국균학회 2020 Mycobiology Vol.48 No.2
Root-feeding Scarabaeidae, particularly white grubs are considered among the most harmful coleopteran insect pests in turfgrass. In this work, sixteen entomopathogenic fungal species were assayed against flower chafer beetle, Protaetia brevitarsis (Coleoptera: Scarabaeidae) and Metarhizium anisopliae JEF-314 showed high virulence. The control ability of the isolate JEF-314 has been in detail tested for a model insect flower chafer beetle. Further analyses showed insect stage-dependent virulence where the fungal virulence was the highest against smaller instar larvae. Additionally, we confirmed that millet-based solid cultured granule was effective against the soil-dwelling larval stage. The isolate also showed a similar ability for a representative pest (Popillia spp.) in laboratory conditions. Our results clearly suggest a high potential of M. anisopliae JEF-314 to control the flower chafer beetle, possibly resulting in controlling of root-feeding white grubs in turfgrass. Based on the insect life cycle and susceptibility to the fungus, late spring and summer time would be the optimum time to apply JEF-314 granules for an effective control. Further characterization of the efficacy of the fungus under field conditions against the Scarabaeidae beetles might provide an efficient tool to control this beetle in an environment-friendly way.