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칼슘제 수관살포가 참다래의 과실 품질과 저장에 미치는 영향
임경호,나양기,임동근,마경철,조윤섭,김월수,이상현,박용서 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-
This study were carried out to improve Kiwifruit quality and storage life. Three kinds of calcium compound were sprayed and calcium content of fruits, weight loss during fruit storage and fruit quality were investigated. Calcium contents within leaves and fruit were lower in Clef-non treatment than that of control. The calcium content in fruit pericarp of Kalk-H and CaCl2 was 0.04 to 0.05% higher than that of control. Fruit weight and soluble solids content at harvest was a little higher but acidity and fruit hardness was lowered. Fruit weight loss of Kalk-H and CaCl2 treatment was 1.39 to 1.53% lower than that of control during storage. The soluble solids of ripen fruit was 1.0 to 1.3% higher in all treatment and 0.8% higher in Kalk-H treatment in 120 of after storage. Fruit hardness of control fruit was higher at harvest but that of CaCl2 treatmented fruit was 0.39㎏/φ 5㎜ higher in 120 days of storage.
Kim, Min-Kyeong,Song, Ji-Yang,Koh, Dong-In,Kim, Jin Young,Hatano, Masahiko,Jeon, Bu-Nam,Kim, Min-Young,Cho, Su-Yeon,Kim, Kyung-Sup,Hur, Man-Wook American Society for Biochemistry and Molecular Bi 2019 The Journal of biological chemistry Vol.294 No.1
<P>Even in the face of physiological DNA damage or expression of the tumor suppressor protein p53, B cell CLL/lymphoma 6 (BCL6) increases proliferation and antagonizes apoptotic responses in B cells. BCL6 represses <I>TP53</I> transcription and also appears to inactivate p53 at the protein level, and additional findings have suggested negative mutual regulation between BCL6 and p53. Here, using <I>Bcl6</I><SUP>−/−</SUP> knockout mice, HEK293A and HCT116 <I>p53</I><SUP>−/−</SUP> cells, and site-directed mutagenesis, we found that BCL6 interacts with p53 and thereby inhibits acetylation of Lys-132 in p53 by E1A-binding protein p300 (p300), a modification that normally occurs upon DNA damage–induced cellular stress and whose abrogation by BCL6 diminished transcriptional activation of p53 target genes, including that encoding caspase-1. Conversely, we also found that BCL6 protein is degraded via p53-induced, caspase-mediated proteolytic cleavage, and the formation of a BCL6–p53–caspase-1 complex. Our results suggest that p53 may block oncogenic transformation by decreasing BCL6 stability via caspase-1 up-regulation, whereas aberrant BCL6 expression inactivates transactivation of p53 target genes, either by inhibiting p53 acetylation by p300 or repressing <I>TP53</I> gene transcription. These findings have implications for B cell development and lymphomagenesis.</P>
Kim, Byong-Kak,Choi, Eung-Chil,Chung, Kyeong-Soo,Park, Hee-Ju,Kim, Hye-Ryoung,Kim, Yang-Sup,Park, Yong-Hwan,Shim, Mi-Ja The Pharmaceutical Society of Korea 1983 Archives of Pharmacal Research Vol.6 No.2
To find anititumor metabolites in Korean basidiomycetes, the shake-cultured mycelia of eight of the higher fungi were extracted with hot water and the extracts, after being partially purified, were subjected to in vivo antitumor test. When administered i. p. at the dose of 30mg/kg/day for ten consecutive days into the female ICR mice, which had been implanted with $1{\times}10^{6}$ / cells of sarcoma 180 twenty four hours before the first injection, the extracts of Agaricus campestris, Lyophyllum decastes, Lyophyllum ulmarium, Armillaria Tabescence and Calvatia exipuliformis respectively showed inhibition ratios of 64.1%, 65.45, 60.-%, 53.0 and 49.3%. These five species were selected for further study, whereas the extracts of Phallus impudicus, Coprinus comatus and Pholiota squarrosa whih showed the inhibition ratios of 31.2%, 33.5% and 19.0% were discontinued.
Bioinspired Synthesis of Hierarchical Silica Nanocage Using an Engineered Capsid Protein
Kyeong Rok KIM,Chang Sup KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Silica nanoparticles have received increasing attention as an ideal candidate for biomedical application due to their excellent biocompatibility, chemical stability, easy surface modification, and high loading capacity. Nano-particles with hierarchical surfaces have tremendous advantages compared to flat surfaces as a carrier in biomedical applications, including large surface area, prolonged blood circulation time, and high binding efficiency to cancer cells, compared to flat nanoparticles. To date, chemical synthesis method has been used to make hierarchical silica nanoparticles, but this method has some limitations, such as the use of harsh conditions, the difficulty of removing a surfactant, and the complicated process. In this study, we have developed silica nanoparticles with the hierarchical surface through a combination of two bioinspired approaches, controlled silica mineralization of diatom and hierarchical structure of human papillomavirus (HPV) 16. A novel fusion protein, HPV 16 L1-R5, was prepared by genetically inserted HPV 16 capsid protein L1 with silica-forming R5 peptide from a diatom Cylindrotheca fusiformis. HPV16 L1-R5 proteins self-assembled into virus-like particles (VLPs) identical with native HPV 16. Hierarchical HPV16 L1-R5 VLP@SiNPs were developed with a size of ca. 60-80 ㎚ under an optimized condition of diatom-inspired silicification. HPV16 L1-R5 VLP@SiNPs encapsulated therapeutic compound and exhibited high cellular uptake efficiency compared to flat mesoporous SiNPs. We expect that our silica nanoparticle with a hierarchical surface could be used as an effective carrier for biomedical application.
KIM, MIN HWAN,PARK, JI AE,WOO, SANG-KEUN,LEE, KYO CHUL,AN, GWANG IL,KIM, BYOUNG SOO,KIM, KWANG IL,LEE, TAE SUP,KIM, CHAN WHA,KIM, KYEONG MIN,KANG, JOO HYUN,LEE, YONG JIN Spandidos Publications 2015 International journal of oncology Vol.46 No.3
<P>Gastrin-releasing peptide receptor (GRPR) is overexpressed by a variety of human tumors and in particular, identified to be upregulated in prostate cancers. The current study aimed to develop clinically translatable BBN analogue-based radioligands for positron emission tomography (PET) of GRPR-positive tumors. We developed radiolabeled BBN analogues and modified radiolabeled galacto-BBN analogues and then investigated their tumor-targeting efficacy in vivo. The chelator 1,4,7-triazacyclononane, 1-glutaric acid-4,7 acetic acid (NODAGA) was used to radiolabel the peptides with Cu-64. The peptides were evaluated by measuring cell-based receptor-binding affinities. Biodistribution experiments and small animal imaging using PET were performed in nude mice bearing subcutaneous PC3 human prostate cancer xenografts. The conjugates were radiolabeled with yields >99%. The stability assay showed that [Cu-64] NODAGA-BBN and [Cu-64]NODAGA-galacto-BBN remained stable in both human and mouse serum for 1 h at 37 degrees C. PET images of PC3 tumor-bearing nude mice were acquired at 1, 3, 24, 48 and 72 h after injection. [64Cu]NODAGA-galacto-BBN showed retention in tumors for 72 h, low liver uptake, and rapid renal clearance. PET imaging results were also confirmed by biodistrubution 1 and 3 h after injection. [Cu-64]NODAGA-BBN and [Cu-64]NODAGA-galacto-BBN are promising new PET probes for GRPR-positive prostate cancer.</P>
Kim, Min-Kyeong,Jeon, Bu-Nam,Koh, Dong-In,Kim, Kyung-Sup,Park, So-Yoon,Yun, Chae-Ok,Hur, Man-Wook American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.10
<P>The human POZ domain and Krüppel-like zinc finger (POK) family proteins play important roles in the regulation of apoptosis, cell proliferation, differentiation, development, oncogenesis, and tumor suppression. A novel POK family transcription factor, BTB/POZ and zinc finger domains factor on chromosome 1 (BOZF-1; also called ZBTB8A), contains a POZ domain and two C<SUB>2</SUB>H<SUB>2</SUB>-type Krüppel-like zinc fingers and is localized at nuclear speckles. Compared with paired normal tissues, BOZF1 expression is increased in cancer tissues of the prostate, breast, and cervix. BOZF1 repressed the transcription of <I>p21WAF</I>/<I>CDKN1A</I> by acting on the proximal promoter concentrated with Sp1-binding GC boxes. BOZF1 competed with Sp1 in binding to GC boxes 1–5/6 of the <I>CDKN1A</I> proximal promoter. In addition, BOZF1 interacted with p53 and decreased the acetylation of p53 by p300, which reduced the DNA binding activity of p53 at the far distal p53-binding element. BOZF1 blocked the two major molecular events that are important in both constitutive and inducible transcription activation of <I>CDKN1A</I>. BOZF1 is unique in that it bound to all the proximal GC boxes to repress transcription, and it inhibited p53 acetylation without affecting p53 stability. BOZF1 might be a novel proto-oncoprotein that stimulates cell proliferation.</P>
PPAR-α Activation Mediates Innate Host Defense through Induction of TFEB and Lipid Catabolism
Kim, Yi Sak,Lee, Hye-Mi,Kim, Jin Kyung,Yang, Chul-Su,Kim, Tae Sung,Jung, Mingyu,Jin, Hyo Sun,Kim, Sup,Jang, Jichan,Oh, Goo Taeg,Kim, Jin-Man,Jo, Eun-Kyeong American Association of Immunologists 2017 Journal of Immunology Vol. No.
<P>The role of peroxisome proliferator-activated receptor a (PPAR-alpha) in innate host defense is largely unknown. In this study, we show that PPAR-alpha is essential for antimycobacterial responses via activation of transcription factor EB (TFEB) transcription and inhibition of lipid body formation. PPAR-alpha deficiency resulted in an increased bacterial load and exaggerated inflammatory responses during mycobacterial infection. PPAR-alpha agonists promoted autophagy, lysosomal biogenesis, phagosomal maturation, and antimicrobial defense against Mycobacterium tuberculosis or M. bovis bacillus Calmette-Guerin. PPAR-alpha agonists regulated multiple genes involved in autophagy and lysosomal biogenesis, including Lamp2, Rab7, and Tfeb in bone marrow-derived macrophages. Silencing of TFEB reduced phagosomal maturation and antimicrobial responses, but increased macrophage inflammatory responses during mycobacterial infection. Moreover, PPAR-alpha activation promoted lipid catabolism and fatty acid beta-oxidation in macrophages during mycobacterial infection. Taken together, our data indicate that PPAR-alpha mediates antimicrobial responses to mycobacterial infection by inducing TFEB and lipid catabolism.</P>
Temperature Dependence of Magnetization of Amorphous TM70Cr5Si10B15 (TM = Fe, CO, Ni) Alloys
Kyeong-Sup Kim,Seong-Cho Yu,Woo-Young Lim,Wha-Nam Myuong 한국자기학회 1997 Journal of Magnetics Vol.2 No.4
We report the salient features of the magnetic properties of amorphous TM_(70)Cr_5Si_(10)B_(15) (TM=Fe, CO, Ni) alloys. The temperature dependence of magnetization for amorphous ribbons were measured by a SQUID and a VSM from 5 K to 700 K under an external field of 10 kOe. Except TM_(70)Cr_5Si_(10)B_(15) that shows a paramagnetic behaviour, both Fe and Co based amorphous alloys show a typical ferromagnetic thermo-magnetization curves. For these two ferromagnetic alloys, the saturation magnetization in the temperature range from 5 K to about 0.4 Tc can be descrived by the Bloch relation, Ms (T) = Ms(0) [1-BT^(3/2)-CT^(5/2)]. The spin wave stiffness constants and the range of exchange interaction were analyzed from the magnetization behaviour. The variation of the magnetic properties are discussed and compared with the composition of the alloys.