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Seo, Jung Seon,Jun, Se Mo,Kwon, Sang Wook,Oh, Il-Hoan,Kim, Tai-Gyu,Lee, Suk Kyeong D.A. Spandidos 2010 International journal of molecular medicine Vol.25 No.1
<P>Although Epstein-Barr virus (EBV) has been detected in 5-15% of gastric carcinoma (GC) cases, the mechanism by which EBV contributes to its tumorigenesis remains unclear. Only a subset of EBV latent proteins such as EBV nuclear antigen-1 and latent membrane protein 2A (LMP2A) are expressed in EBV-associated GC (EBVaGC) cases. In this study, to elucidate the role of LMP2A in the tumorigenesis of EBVaGC, we established permanent cell lines expressing LMP2A. The LMP2A gene was cloned from a naturally EBV-infected EBVaGC cell line, SNU-719 and transduced into an EBV-negative GC cell line, AGS, using a retroviral vector. The sequence of SNU-719 LMP2A showed several conserved variations compared to that of the prototype EBV strain B95-8 LMP2A. Four of seven established cell clones expressed LMP2A protein at detectable levels. These cell clones did not show enhanced cell growth compared to control cells in normal or low serum-containing medium. Furthermore, LMP2A expression had no effect on colony forming ability of the cell clones in soft agar. Our results suggest that LMP2A alone has little effect on tumorigenesis of EBVaGC.</P>
Seo, Dae-Hyun,Yoon, Kyeong-Wook,Lee, Sang Koo,Kim, Young-Jin The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.55 No.2
Perineurial cysts (Tarlov cysts) are lesions of the nerve root that are often observed in the sacral area. There is debate about whether symptomatic perineurial cysts should be treated surgically. We presented three patients with symptomatic perineurial cyst who were treated surgically, and introduced sacral recapping laminectomy. Patients complained of low back pain and hypesthesia on lower extremities. We performed operations with sacral recapping technique for all three. The outcome measure was baseline visual analogue score and post operative follow up magnetic resonance images. All patients were completely relieved of symptoms after operation. Although not sufficient to address controversies, this small case series introduces successful use of a particular surgical technique to treat sacral perineural cyst, with resolution of most symptoms and no sequelae.
( Seo Hwa Kim ),( Moon Seong Baek ),( Dong Sik Yoon ),( Jong Seol Park ),( Byoung Wook Yoon ),( Byoung Su Oh ),( Jin Kyeong Park ),( Hui Jung Kim ) 대한결핵 및 호흡기학회 2014 Tuberculosis and Respiratory Diseases Vol.77 No.2
Background: Low levels of serum vitamin D is associated with several lung diseases. The production and activation of matrix metalloproteinases (MMPs) may play an important role in the pathogenesis of emphysema. The aim of the current study therefore is to investigate if vitamin D modulates the expression and activation of MMP-2 and MMP-9 in human lung fibroblasts (HFL-1) cells. Methods: HFL-1 cells were cast into three-dimensional collagen gels and stimulated with or without interleukin-1β (IL-1β) in the presence or absence of 100 nM 25-hydroxyvitamin D (25(OH)D) or 1,25-dihydroxyvitamin D (1,25(OH)2D) for 48 hours. Trypsin was then added into the culture medium in order to activate MMPs. To investigate the activity of MMP-2 and MMP-9, gelatin zymography was performed. The expression of the tissue inhibitor of metalloproteinase (TIMP-1, TIMP-2) was measured by enzyme-linked immunosorbent assay. Expression of MMP-9 mRNA and TIMP-1, TIMP-2 mRNA was quantified by real time reverse transcription polymerase chain reaction. Results: IL-1β significantly stimulated MMP-9 production and mRNA expression. Trypsin converted latent MMP-2 and MMP-9 into their active forms of MMP-2 (66 kDa) and MMP-9 (82 kDa) within 24 hours. This conversion was significantly inhibited by 25(OH)D (100 nM) and 1,25(OH)2D (100 nM). The expression of MMP-9 mRNA was also significantly inhibited by 25(OH)D and 1,25(OH)2D. Conclusion: Vitamin D, 25(OH)D, and 1,25(OH)2D play a role in regulating human lung fibroblast functions in wound repair and tissue remodeling through not only inhibiting IL-1β stimulated MMP-9 production and conversion to its active β inhibition on TIMP-1 and TIMP-2 production.
Choi, Seo-Hyun,Koh, Dong-In,Cho, Su-Yeon,Kim, Min-Kyeong,Kim, Kyung-Sup,Hur, Man-Wook American Society for Biochemistry and Molecular Bi 2019 The Journal of biological chemistry Vol.294 No.35
<P>Transcriptional regulator KAISO plays a critical role in cell cycle arrest and apoptosis through modulation of p53 acetylation by histone acetyltransferase p300. KAISO potently stimulates apoptosis in cells expressing WT p53, but not in p53-mutant or p53-null cells. Here, we investigated how KAISO transcription is regulated by p53, finding four potential p53-binding sites (p53-responsive DNA elements; p53REs) located in a distal 5′-upstream regulatory element, intron 1, exon 2 coding sequence, and a 3′-UTR region. Transient transcription assays of pG5-p53RE-Luc constructs with various p53REs revealed that p53 activates <I>KAISO (ZBTB33)</I> transcription by acting on p53RE1 (−4326 to −4227) of the 5′-upstream region and on p53RE3 (+2929 to +2959) of the exon 2 coding region during early DNA damage responses (DDRs). ChIP and oligonucleotide pulldown assays further disclosed that p53 binds to the p53RE1 and p53RE3 sites. Moreover, ataxia telangiectasia mutated (ATM) or ATM-Rad3–related (ATR) kinase-mediated p53 phosphorylation at Ser-15 or Ser-37 residues activated <I>KAISO</I> transcription by binding its p53RE1 or p53RE3 sites during early DDR. p53RE1 uniquely contained three p53-binding half-sites, a structural feature important for transcriptional activation by phosphorylated p53 Ser-15·Ser-37. During the later DDR phase, a KAISO-mediated acetylated p53 form (represented by a p53QRQ acetyl-mimic) robustly activated transcription by acting on p53RE1 in which this structural feature is not significant, but it provided sufficient KAISO levels to confer a p53 “apoptotic code.” These results suggest that the critical apoptosis regulator <I>KAISO</I> is a p53 target gene that is differently regulated by phosphorylated p53 or acetylated p53, depending on DDR stage.</P>
Hypoxia-induced RelA/p65 derepresses <i>SLC16A3 (MCT4)</i> by downregulating <i>ZBTB7A</i>
Choi, Seo-Hyun,Kim, Min-Young,Yoon, Young-So,Koh, Dong-In,Kim, Min-Kyeong,Cho, Su-Yeon,Kim, Kyung-Sup,Hur, Man-Wook Elsevier 2019 Biochimica et biophysica acta. Gene regulatory mec Vol.1862 No.8
<P><B>Abstract</B></P> <P>Overexpressed Solute Carrier Family 16 Member 3 (SLC16A3, also called MCT4) plays a critical role in hypoxic cancer cell growth and proliferation, by expelling glycolysis-derived lactate across the plasma membrane. However, how <I>SLC16A3</I> expression is regulated, under hypoxic conditions, is poorly understood. FBI-1, encoded by <I>ZBTB7A</I>, is a proto-oncoprotein. Interestingly, under hypoxic conditions, expression of <I>SLC16A3</I>, and hypoxia-inducible factor-1 (HIF-1), increased gradually, while FBI-1 expression decreased, suggesting a negative correlation between SLC16A3/HIF-1 and FBI-1 expression. Consequently, we hypothesized that FBI-1 might regulate SLC16A3 and/or HIF-1 expression.</P> <P>Transient transfection and transcription assays of <I>SLC16A3</I> promoter reporter fusion constructs, oligonucleotide-pulldowns, and ChIP assays, showed that HIF-1α activates <I>SLC16A3</I> by binding to a hypoxia-response element (HRE), while ectopic FBI-1 potently repressed <I>SLC16A3</I>, by binding to both FBI-1-response elements (FREs) and HREs, during hypoxia. Further evidence for this model was downregulation of <I>ZBTB7A</I>, correlated with <I>SLC16A3</I> upregulation, in hypoxic colon cancer cells.</P> <P>We also investigated how FBI-1 expression is downregulated during hypoxia. The 5′-upstream regulatory region of <I>ZBTB7A</I> contains two NF-κB-binding sites and two HREs. Interestingly, hypoxia activated NF-κB (RelA/p65) and also increased its nuclear translocation. NF-κB repressed <I>ZBTB7A</I> by binding NF-κB-binding elements, and downregulated the repressor FBI-1, thereby increasing <I>SLC16A3</I> transcription. While transcriptional repression of <I>SLC16A3</I> by FBI-1 inhibited lactate efflux, repression of <I>ZBTB7A</I> and activation of lactate efflux by NF-κB, increased colon cancer cell growth and proliferation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> While FBI-1 expression was decreased under hypoxic condition, SLC16A3 and HIF-1a expression was increased gradually. </LI> <LI> HIF-1α activated <I>SLC16A3</I> while ectopic FBI-1 potently repressed <I>SLC16A3</I> during hypoxia. </LI> <LI> Hypoxia activated NF-κB (RelA/p65) repressed <I>ZBTB7A</I>, thereby increasing <I>SLC16A3</I> transcription. </LI> </UL> </P>
항측용 디지털카메라의 Direct Georeferencing을 위한 테스트사이트별 정확도 평가
서상일(Seo, Sang Il),소재경(So, Jae Kyeong),박병욱(Park, Byung-Wook),이병길(Lee, Byoungkil) 한국측량학회 2014 한국측량학회 학술대회자료집 Vol.2014 No.4
최근 항공측량분야에는 빠르고 정확한 공간정보 취득을 위한 Direct Georeferencing 기술이 보편화 되면서 Boresight Calibration이 중요하게 요구되고 있다. 하지만 Boresight Calibration은 지상기준점의 배치와 수, 정확도에 따라 결과가 다를 수 있다. 본 연구에서는 서로 다른 검정장에서 Boresight Calibration 수행하여 얻은 검정결과를 이용하여 Direct Georeferencing기법에 의한 외부표정요소 직접결정을 통해 정사영상을 제작하여 각각의 정확도를 평가하였다. Recently, Direct Georeferencing technology has been widely utilized for acquirement of fast and accurate spatial information in the field of aerial survey, thus required Boresight Calibration. But the results of Boresight Calibration have different results according to distribution and counts of GCPs. In this study, Direct Georeferencing based orthophoto generation is generated by determine of exterior orientation parameters of Boresight Calibration in different Test-sites, and assessment of geometric accuracy.
Cho, Byung-Wook,Oliveros, Maria Cynthia,Park, Kyoung-Mi,Do, Kyoung-Tag,Lee, Ki-Hwan,Seo, Kang-Seok,Choi, Jae-Gwan,Lee, Moon-Jun,Cho, In-Kyung,Choi, Byoung-Chul,Ryu, Kyeong-Seon,Hwang, In-Ho Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.6
The aim of the present study was to evaluate the variation in ultimate pH of commercial populations of pure-breed (Landrace, Duroc and Yorkshire) pig's longissimus muscles and their effect on objective meat quality traits and sensory characteristics. Fifty boars were sampled from 184 pigs, which were reared at three breeding farms and slaughtered at a commercial abattoir. The selection was determined based on ultimate pH, and animals were segregated into three groups: low pH (pH $\leq$ 5.5, n=13), medium pH (pH 5.5 to 5.6, n=18) and high pH (pH $\geq$ 5.6, n=16). The breeds had no significant effects; however, pigs with a higher ultimate pH had significantly (p<0.05) higher intramuscular fat content, lower level of polyunsaturated fatty acids, lower level of lipid oxidation and higher eating quality compared to those with lower ultimate pH. As the ultimate pH increased, the relative proportion of C14:0, C16:0 and C18:1 increased while C18:2n6 and C20:4n6 decreased. The present study demonstrates that the economic value of pigs can be characterized by the ultimate pH and/or intramuscular fat content. However, these results do not necessarily indicate that a high ultimate pH directly corresponds to high intramuscular fat content and vice versa.