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RISS 인기검색어
- 검색키워드
- 저자 : Kwanseop Lee (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Detection of an intermediate during the unfolding process of the dimeric ketosteroid isomerase
Jang, Do Soo,Lee, Hyeong Ju,Lee, Byeongdu,Hong, Bee Hak,Cha, Hyung Jin,Yoon, Jinhwan,Lim, Kwanseop,Yoon, Ye Jeong,Kim, Jehan,Ree, Moonhor,Lee, Hee Cheon,Choi, Kwan Yong Elsevier 2006 FEBS letters Vol.580 No.17
<P><B>Abstract</B></P><P>Failure to detect the intermediate in spite of its existence often leads to the conclusion that two-state transition in the unfolding process of the protein can be justified. In contrast to the previous equilibrium unfolding experiment fitted to a two-state model by circular dichroism and fluorescence spectroscopies, an equilibrium unfolding intermediate of a dimeric ketosteroid isomerase (KSI) could be detected by small angle X-ray scattering (SAXS) and analytical ultracentrifugation. The sizes of KSI were determined to be 18.7Å in 0M urea, 17.3Å in 5.2M urea, and 25.1Å in 7M urea by SAXS. The size of KSI in 5.2M urea was significantly decreased compared with those in 0M and 7M urea, suggesting the existence of a compact intermediate. Sedimentation velocity as obtained by ultracentrifugation confirmed that KSI in 5.2M urea is distinctly different from native and fully-unfolded forms. The sizes measured by pulse field gradient nuclear magnetic resonance (NMR) spectroscopy were consistent with those obtained by SAXS. Discrepancy of equilibrium unfolding studies between size measurement methods and optical spectroscopies might be due to the failure in detecting the intermediate by optical spectroscopic methods. Further characterization of the intermediate using <SUP>1</SUP>H NMR spectroscopy and Kratky plot supported the existence of a partially-folded form of KSI which is distinct from those of native and fully-unfolded KSIs. Taken together, our results suggest that the formation of a compact intermediate should precede the association of monomers prior to the dimerization process during the folding of KSI.</P>
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분광측색계, 색차계의 색 수치 값을 이용한 타이타늄 산화막의 두께 정량화
이다영(Dayoung Lee),한아영(Ayoung Han),하동흔(Dongheun Ha),유현석(Hyeonseok Yoo),김훈식(Hunsik Kim),정나겸(Nagyeom Jung),장관섭(Kwanseop Jang),최진섭(Jinsub Choi) 한국표면공학회 2018 한국표면공학회지 Vol.51 No.3
The anodic TiO₂ layers which are prepared in various anodization conditions exhibit their specific color depending on the thickness of TiO₂. In this study, the relationship between the color of TiO₂ layer, which is grown by PEO (Plasma electrolytic oxidation), and the thickness of the TiO₂ layer is investigated. To evaluate the color change of the TiO₂ layer, the value of color (dE<SUP>*</SUP>ab) is measured and calculated by spectrophotometer and chromameter. As a result, it is found that dE<SUP>*</SUP>ab values and thickness of TiO₂ layers form a linear relationship with meaningful formular. This formula can be helpful to quantify the thickness of the TiO₂ layer by the numerical dE<SUP>*</SUP>ab values. In this process, the spectrophotometer shows more precise results than the chromameter dose. If fluoride ions (F-) are included in the electrolyte, it will affect the dE<SUP>*</SUP>ab values of the TiO₂. layer. This is against the propensity, which is analyzed by XRD (X-ray diffraction) and XPS (X-ray photoelectron spectroscopy). It is important that the formular suggested in this study provides other metals which can be also anodized with the possibility of quantifying the thickness of the TiO₂ layer by the dE<SUP>*</SUP>ab values.
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Acute pancreatitis in hand, foot and mouth disease caused by Coxsackievirus A16: case report
Park, Byungsung,Kwon, Hyuckjin,Lee, Kwanseop,Kang, Minjae The Korean Pediatric Society 2017 Clinical and Experimental Pediatrics (CEP) Vol.60 No.10
Coxsackievirus A16 (CA16), which primarily causes hand, foot, and mouth disease (HFMD), is associated with complications, such as encephalitis, acute flaccid paralysis, myocarditis, pericarditis, and shock. However, no case of pancreatitis associated with CA16 has been reported in children. We report a case of CA16-associated acute pancreatitis in a 3-year-old girl with HFMD. She was admitted because of poor oral intake and high fever for 1 day. Maculopapular rashes on both hands and feet and multiple vesicles on the soft palate were observed on physical examination. She was treated conservatively with intravenous fluids. On the fourth hospital day, she had severe abdominal pain and vomiting. The serum levels of amylase and lipase were remarkably elevated (amylase, 1,902 IU/L; reference range, 28-100 IU/L; lipase, >1,500 IU/L; reference range, 13-60 IU/L), and ultrasonography showed diffuse swelling of the pancreas with a small amount of ascites. The real-time reverse transcription polymerase chain reaction result from a stool sample was positive for CA16. CA16 can cause acute pancreatitis, and should be considered in the differential diagnosis of abdominal pain in children with HFMD.
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In-Gyu Kim,Byung Seup Kim,Jang Yong Jeon,Jae Woo Kwon,Joo Seop Kim,Doo Jin Kim,Jae Pil Jung,Seong Eun Chon,Han Joon Kim,Eui Yong Jeon,Min-Jeong Kim,Kwanseop Lee 한국간담췌외과학회 2011 한국간담췌외과학회지 Vol.15 No.3
Liver transplantation with preservation of the recipient vena cava (piggyback technique) has been performed as an alternative to the conventional method. Outflow disturbance or obstruction of the vena cava in the early period after liver transplantation is associated with high morbidity and mortality. We used side-to-side cavo-caval anastomosis (modified piggyback technique) in a deceased-donor liver transplantation (DDLT) for venous outflow reconstruction. On postoperative day 9, the patient developed abdominal discomfort, and abnormal liver function showing serum total bilirubin of 6.2 mg/dl and serum AST/ALT of 297/597 IU/L. Doppler ultrasound showed mono-phasic wave forms of the hepatic vein. Computed tomography showed focal narrowing of 9.5 mm±2 mm in diameter at the cavo-caval anastomosis site. Liver biopsy was showed that there was no evidence of acute allograft rejection. Direct venogram showed stenosis of the cavo-caval anastomosis with a pressure gradient of 12 mmHg. An interventional stent was inserted in the stenotic site of the inferior vena cava, and the pressure gradient decreased to 2 mmHg. He was discharged from hospital on postoperative day 23 without any other complications. Herein we report a case of deceased-donor liver transplantation using the modified piggyback technique, who received an inferior vena cava stent due to stricture of the reconstructed orifice of the vena cava. (Korean J Hepatobiliary Pancreat Surg 2011;15:184-188)
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