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RISS 인기검색어
- 검색키워드
- 저자 : Krohn Inge Kortekaas (검색결과 2 건)
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The Influence of IgE on Cultured Human Mast Cells
Pernille Munk Frandsen,Inge Jacoba Maria Kortekaas Krohn,Hans Jürgen Hoffmann,Peter Oluf Schiøtz 대한천식알레르기학회 2013 Allergy, Asthma & Immunology Research Vol.5 No.6
Purpose: The mast cell plays a pivotal role in the human immune response. Crosslinking of 2 IgE molecules bound to the high affinity IgE receptor(FcεRI) on the surface of the mast cell results in mast cell degranulation and the release of several proinflammatory mediators. Patients with type-I allergyhave increased levels of IgE in the blood compared to healthy individuals. Methods: In a 6-week culture system of stem cells to human mastcells we investigated the effect of the concentration of IgE. The mast cells were cultured with different concentrations of IgE for the last 10 days ofthe maturation period. It was observed how the IgE concentration affects the histamine release, FcεRI density on the mast cell surface and the concentrationof other mediators. Results: A clear correlation between IgE concentration in culture medium and the release of histamine upon activationwas observed. It showed a bell-shaped dose response curve, with maximal response around an IgE-concentration of 250 ng/mL. Furthermore,the sensitivity of the mast cells and surface density of FcεRI on mast cell surface was also influenced by the IgE concentration in the culture medium. Conclusions: IgE in the culture medium during the last 10 days of mast cell maturation influences the release of the preformed mediator histamineafter mast cell activation and the density of FcεRI on the mast cell surface. The release of the de novo synthetized mediator prostaglandin D2and the expression of chymase and tryptase are not influenced by IgE in culture medium.
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Huygen Lisa,Thys Pauline Marie,Wollenberg Andreas,Gutermuth Jan,Krohn Inge Kortekaas 대한피부과학회 2024 Annals of Dermatology Vol.36 No.2
Background: Skin barrier function assessment is commonly done by measuring transepider- mal water loss (TEWL). An important limitation of this method is the influence of intrinsic and extrinsic factors. Electrical impedance spectroscopy (EIS) is a lesser-established method for skin barrier function assessment. Some influential factors have been described, but no guidelines exist regarding the standardization of these measurements. Objective: To evaluate the effect size of daily routine activities on TEWL and EIS, as well as their correlation with age and anatomical differences. Methods: Healthy participants (n=31) were stratified into three age groups (18–29, 30–49, and ≥50 years). In a climate-controlled room, EIS and TEWL measurements were performed on the left and right volar forearm and abdomen. Results: Body cream application decreased TEWL and EIS values after 15 and 90 minutes. Skin washing decreased TEWL for 15 minutes and EIS values for at least 90 minutes. TEWL was in- creased 5 minutes after moderate to intense exercise. Coffee intake increased TEWL on the ab- domen after 60 minutes. TEWL and EIS values did not correlate with participants’ age and no anatomical differences were obser ved. No correlation was obser ved between TEWL and EIS. Conclusion: Body cream application and skin washing should be avoided at least 90 minutes prior to measurements of TEWL and EIS. Exercise and coffee intake should also be avoided prior to TEWL measurements. EIS may be a promising tool for skin barrier function assess- ment as it is less affected by daily routine activities than TEWL.
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